Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: INDEX OF VOLUME 54 (2018) - AUTHORS INDEX - AUTHORS INSTITUTIONS INDEX Journal: Plant Protection Science Pages: I-VI Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/11235-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/11235-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:11235-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Thanh Mai Nguyen Author-Workplace-Name: Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam Author-Name: Nga Thi Thanh Le Author-Workplace-Name: Lam Dong Crop Production and Plant Protection Sub-Department, Da Lat City, Vietnam Author-Name: Jouni Havukainen Author-Workplace-Name: Laboratory of Environmental Engineering, Lappeenranta University of Technology, Lappeenranta, Finland Author-Name: David B. Hannaway Author-Workplace-Name: Department of Crop and Soil Science, Oregon State University, Corvallis, USA$2 Title: Pesticide use in vegetable production: A survey of Vietnamese farmers' knowledge Abstract: Concerns about inappropriate storage, application rates, and disposal practices of pesticides prompted this case study of Vietnamese farmers' knowledge, attitudes, and practices. 128 small-scale vegetable growers in Lam Dong Province were included in field surveys, questionnaires, and interviews. Farmers reported inappropriate mixing of pesticides and disposal methods. Many also reported ill-timed applications posing potential hazards to the human health and environment. Improved training and monitoring of pesticide residues on foodstuffs and in agricultural soils and community water supplies are needed to ensure safe farmer practices. Community-based training and education, jointly funded by local, national, and international agricultural production and food safety groups, would be a cost-effective method of minimising pesticide applications and improving food safety. Keywords: pesticide application, pesticide disposal, vegetable pesticide residues, environmental protection, agricultural production, Vietnam agriculture Journal: Plant Protection Science Pages: 203-214 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/69/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/69/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0002.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:69-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Adel M. Zakri Author-Name: Abdullah A. Al-Doss Author-Workplace-Name: Department of Plant Production, 3Department of Animal Production and 4Department of Plant Protection, College of Food & Author-Name: Markus Sack Author-Workplace-Name: Agriculture Science, King Saud University, Riyadh, Saudi Arabia Author-Name: Ahmed A. Ali Author-Workplace-Name: Department of Plant Production, 3Department of Animal Production and 4Department of Plant Protection, College of Food & Author-Name: Emad M. Samara Author-Workplace-Name: Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany Author-Name: Basem S. Ahmed Author-Workplace-Name: Department of Plant Production, 3Department of Animal Production and 4Department of Plant Protection, College of Food & Author-Name: Mahmoud. A. Amer Author-Name: Omar. A. Abdalla Author-Name: Mohammed A. Al-Saleh Title: Cloning and characterisation of nanobodies against the coat protein of Zucchini yellow mosaic virus Abstract: Zucchini yellow mosaic virus (ZYMV), in the family Potyviridae, causes an economically important disease. Antibodies are valuable reagents for diagnostic assays to rapidly detect viral infection. Here, we report the isolation of camel-derived variable domains of the heavy chain antibody (VHH, also called nanobodies) directed against the coat protein (CP) of ZYMV. Several nanobodies that specifically recognise ZYMV-CP were identified. The isolated nanobodies showed binding not only to recombinant ZYMV-CP but also to native ZYMV, indicating that these nanobodies can be used in diagnostic tools to detect viral infections. Keywords: nanobodies, VHH, naï, ve library, phage display Journal: Plant Protection Science Pages: 215-221 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/158/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/158/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0003.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:158-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Mohamad Hamed Ghodoum Parizipour Author-Name: Leila Ramazani Author-Workplace-Name: Plant Protection Department, Ramin University of Agriculture and Natural Resources of Khouzestan, Mollasani, Iran Author-Name: Babak Pakdaman Sardrood Author-Workplace-Name: Plant Protection Department, Ramin University of Agriculture and Natural Resources of Khouzestan, Mollasani, Iran Title: Temperature affected transmission, symptom development and accumulation of Wheat dwarf virus Abstract: One of the biotic agents of yellowing and stunting in wheat and barley cultivations is Wheat dwarf virus (WDV) which is naturally transmitted by the leafhopper Psammotettix alienus (Dahlbom). WDV-Wheat and WDV-Barley isolates of WDV were transmitted to wheat and barley, respectively, using the leafhoppers under four temperature regimes of constant 20, 25, 30, and 35°C. Infection rate, symptom development and virus content of the virus-inoculated plants were determined and the data was statistically analysed. The results showed that the temperature of 25°C was associated with the highest infection rate caused by the viruses. Moreover, P. alienus nymphs were found to be more efficient vectors of WDV than adults, highlighting the importance of nymphs in the epidemiology of wheat dwarf disease. WDV-infected plants incubated at 35°C showed less symptoms than those kept at 20, 25, and 30°C. ELISA results showed that these plants had comparatively low virus content. However, there was no significant difference between the infection rate, symptom development and virus content in plants infected by WDV-Wheat or WDV-Barley. Keywords: geminivirus, Psammotettix alienus, symptom development, epidemiology, cereal Journal: Plant Protection Science Pages: 222-233 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/147/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/147/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0004.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:147-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Dana Šafářová Author-Workplace-Name: Department of Cell Biology and Genetics, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Pavel Lauterer Author-Workplace-Name: Department of Entomology, The Moravian Museum, Brno, Czech Republic Author-Name: Martin Starý Author-Workplace-Name: Department of Cell Biology and Genetics, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Pavla Válová Author-Workplace-Name: Department of Cell Biology and Genetics, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Milan Navrátil Title: Insight into epidemiological importance of phytoplasma vectors in vineyards in South Moravia, Czech Republic Abstract: Bois noir (BN), caused by 'Candidatus Phytoplasma solani', is a serious disease of grapevines in Europe. During the 2010-2012 survey in Perná vineyard (South Moravia, Czech Republic) a total of 4854 insect individuals were collected and among these, 95 insect species belonging to Auchenorrhyncha (77 species), Heteroptera (12), and Psylloidea (62) were indentified. The nested polymerase chain reaction-restriction fragment length polymorphism analyses confirmed Hyalesthes obsoletus as the main BN vector with 43.8% of phytoplasma positive individuals on average. A significant role of Anaceratagallia ribauti (22.6% of phytoplasma positive specimens) should be taken into account based on its occurrence and incidence of infected individuals. Eleven insect species were identified as new carriers of 'Ca. P. solani' or suggested as potential BN vectors in this work. Keywords: 'Candidatus Phytoplasma solani', 'Candidatus Phytoplasma asteris', bois noir, vector, Hyalesthes obsoletus Journal: Plant Protection Science Pages: 234-239 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/8/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/8/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0005.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:8-2018-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Mustafa Mirik Author-Workplace-Name: Department of Plant Protection, Agriculture Faculty, Namik Kemal University, Tekirdag, Turkey Author-Name: Yesim Aysan Author-Workplace-Name: Department of Plant Protection, Agriculture Faculty, Cukurova University, Adana, Turkey Author-Name: Fulya Baysal-Gurel Title: Bacterial spot and blight diseases of ornamental plants caused by different Xanthomonas species in Turkey Abstract: Putative strains belonging to Xanthomonas spp. causing leaf spot and blight diseases on geranium (Pelargonium peltatum and P. hortorum), begonia (Begonia × tuberhybrida), anthurium (Anthurium andraeanum), Chinese hibiscus (Hibiscus rosa-sinensis), and English ivy (Hedera helix) growing in Turkey were isolated. All bacterial strains were classified as Gram-negative, oxidase negative, catalase, levan and starch hydrolysis positive, with hypersensitive reaction positive on tobacco and pathogenic to host plants. Identification of these strains was further confirmed by serological method using ELISA kits, conventional PCR, carbon utilisation, and FAME. Results of the identification showed that 28, 24, 10, 2, and 1 strains were identified as X. axonopodis pv. begoniae, X. hortorum pv. pelargonii, X. axonopodis pv. dieffenbachiae, X. hortorum pv. hederae, and Xanthomonas sp., respectively. This is the first report of X. hortorum pv. hederae on English ivy in Turkey. Keywords: bacteria, detection, ornamentals, Xanthomonas Journal: Plant Protection Science Pages: 240-247 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/10/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/10/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0006.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:10-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Amina KHEIREDDINE Author-Workplace-Name: Laboratory of Mycology, Pathology and Biomarkers, Faculty of Sciences of Tunis, Tunis El Manar University, Tunis, Tunisia Author-Name: Badiâa ESSGHAIER Author-Workplace-Name: Laboratory of Mycology, Pathology and Biomarkers, Faculty of Sciences of Tunis, Tunis El Manar University, Tunis, Tunisia Author-Name: Abdeljabar HEDI Author-Workplace-Name: Laboratory of Mycology, Pathology and Biomarkers, Faculty of Sciences of Tunis, Tunis El Manar University, Tunis, Tunisia Author-Name: Cyrine DHIEB Author-Workplace-Name: Laboratory of Mycology, Pathology and Biomarkers, Faculty of Sciences of Tunis, Tunis El Manar University, Tunis, Tunisia Author-Name: Najla SADFI-ZOUAOUI Title: New epiphytic yeasts able to reduce grey mold disease on apples Abstract: Botrytis cinerea, the causal agent of grey mould, is a predominant agent causing extensive postharvest and quality losses of apples in Tunisia and worldwide. Efforts to manage this disease have met with limited success. For this reason, the use of microorganism preparations to control fungal diseases as an alternative to fungicides became an urgent need. From a total of 60 epiphytic yeasts, 10 were assessed in vitro against B. cinerea and selected isolates showing antagonism were evaluated for their ability to suppress the grey mould in vivo. On Petri plates, the most promising strains (three strains of Aureobasidium pullulans, one Cryptococcus flavescens, and one Citeromyces matritensis) showed a zone of inhibition against the pathogen fungus not exceeding 10 mm. In vivo, these isolates showed a remarkable antifungal activity since they significantly reduced disease severity on apples from 63% to 95% compared to the control. In conclusion, the work has demonstrated that the three strains, L7 of Aureobasidium pullulans, L2 of Citeromyces matritensis, and L10 of Cryptococcus flavescens, were highly effective and can be used as potential biocontrol agents in controlling the post-harvest decay of apples caused by B. cinerea. Keywords: Botrytis cinerea, apples, antagonistic yeasts, biocontrol Journal: Plant Protection Science Pages: 248-257 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/103/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/103/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0007.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:103-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ghulam Ali Bugti Author-Workplace-Name: Provincial Key Laboratory of Microbial Control and Author-Workplace-Name: School of Plant Protection, Anhui Agricultural University, Hefei, P.R. China Author-Name: Cao Na Author-Workplace-Name: Provincial Key Laboratory of Microbial Control and Author-Name: Wang Bin Author-Workplace-Name: Provincial Key Laboratory of Microbial Control and Author-Name: Lin Hua Feng Author-Workplace-Name: School of Plant Protection, Anhui Agricultural University, Hefei, P.R. China Title: Control of plant sap-sucking insects using entomopathogenic fungi Isaria fumosorosea strain (Ifu13a) Abstract: The virulence of the Isaria fumosorosea strain (Ifu13a) against different plant sap-sucking insects such as Jacobiasca formosana Paoli (Hemiptera: Cicadellidae), Aphis gossypii Glover (Hemiptera: Aphididae), Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), and Stephanitis nashi Esaki et Takeya (Hemiptera: Tingidae) in laboratory condition at 21 ± 1°C temperature and 78 ± 5% relative humidity was determined. We found that the Ifu13a strain had excellent potential to control the target insects. The mortality of the tested insect species ranged from 81 to 100% in the concentration of 1 × 108 conidia/ml. However, the lowest mortality of 33% was observed in the concentration of 1 × 105 conidia/ml against the S. nashi population. Median lethal times (LT50) were obtained from a regression-probit value which indicated 4.1, 4.1, 4.8, and 7.3 days at a concentration of 1 × 108 conidia/ml, whereas, median lethal concentration dosages (LC50) were calculated as 3.9 × 103, 6.8 × 104, 3.0 × 104, and 6.9 × 105 conidia/ml against J. formosana, A. gossypii, B. tabaci, and S. nashi, respectively. The present study showed that the Ifu13a fungal strain is highly pathogenic to the target insects, and it can be used as a biocontrol agent against plant sap-sucking insect species under favourable weather conditions. Keywords: microbial control, entomopathogen, Jacobiasca formosana, Aphis gossypii, Bemisia tabaci, Stephanitis nashi Journal: Plant Protection Science Pages: 258-264 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/118/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/118/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0008.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:118-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Dariusz Piesik Author-Name: Didier Rochat Author-Workplace-Name: UMR N°1272 INRA/Université Paris 6/AgroParisTech, Physiologie de l'Insecte: Signalisation et Communication, INRA Centre de Versailles-Grignon, Versailles Cedex, France Author-Name: Jan Bocianowski Author-Workplace-Name: Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland Author-Name: Frédéric Marion-Poll Author-Workplace-Name: UMR N°1272 INRA/Université Paris 6/AgroParisTech, Physiologie de l'Insecte: Signalisation et Communication, INRA Centre de Versailles-Grignon, Versailles Cedex, France Author-Workplace-Name: AgroParisTech, Université Paris-Saclay, Paris, France Title: Repellent activity of plants from the genus Chenopodium to Ostrinia nubilalis larvae Abstract: The olfactory choices expressed by naïve neonate European corn borer (ECB) larvae were tested by recording their locomotor activities in response to odours coming from plants of the genus Chenopodium (producing phytoecdysteroids or not). ECB larvae were found to be repelled from phytoecdysteroid-positive species, except C. album and C. polyspermum. On the contrary, they were mildly attracted or mildly repelled by phytoecdysteroid-negative species, except C. botrys (which emits a rancid odour). These observations indicate that neonate ECB larvae clearly differentiate plant odours and suggest that well-defended plants may emit repellent odours. Keywords: European corn borer, odours, larvae, orientation, behaviour Journal: Plant Protection Science Pages: 265-271 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/143/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/143/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0009.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:143-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ján Kulfan Author-Workplace-Name: Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Slovak Republic Author-Name: Lenka Sarvašová Author-Workplace-Name: Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Slovak Republic Author-Workplace-Name: Faculty of Ecology and Environmental Sciences, Technical University in Zvolen, Zvolen, Slovak Republic Author-Name: Michal Parák Author-Workplace-Name: Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Slovak Republic Author-Name: Marek Dzurenko Author-Workplace-Name: Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Slovak Republic Author-Workplace-Name: Faculty of Ecology and Environmental Sciences, Technical University in Zvolen, Zvolen, Slovak Republic Author-Name: Peter Zach Author-Workplace-Name: Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Slovak Republic Title: Can late flushing trees avoid attack by moth larvae in temperate forests? Abstract: We investigated moth larvae (Lepidoptera) developing in temperate forests in Central Europe shortly after the tree budburst (the "brumata-viridana complex"). Larvae were collected in southern Slovakia in May 2015 and May 2016 from young and mature trees of late flushing Quercus cerris L. and early flushing Q. pubescens Willd. Although Q. cerris yielded fewer species (40 species) than Q. pubescens (47 species), the rarefied number of species and the Chao index suggested a similar number of species on mature trees of both oak species. Both the total number of moth larvae in assemblages and the abundance of dominant species (pests) were significantly lower on Q. cerris than Q. pubescens. The results suggest the release of Q. cerris with delayed budburst from heavy infestations by folivorous moth larvae. Knowledge obtained can be applied in silvicultural and horticultural practices aimed to protect and maintain forest, fruit, and ornamental trees. Keywords: Quercus cerris, Quercus pubescens, folivorous insects, Lepidoptera, phenology, budburst Journal: Plant Protection Science Pages: 272-283 Volume: 54 Issue: 4 Year: 2018 DOI: 10.17221/11/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/11/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201804-0010.txt Handle: RePEc:caa:jnlpps:v:54:y:2018:i:4:id:11-2018-PPS