Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: List of Reviewers 2018 Journal: Plant Protection Science Pages: I-II Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/11542-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/11542-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:11542-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Beata Hasiów-Jaroszewska Author-Name: Daria Budzyńska Author-Workplace-Name: Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Poznań, Poland Author-Name: Natasza Borodynko-Filas Author-Workplace-Name: Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Poznań, Poland Title: Genetic diversity of the Cucumber green mottle mosaic virus and the development of RT-LAMP assay for its detection Abstract: To analyse the genetic diversity of the Cucumber green mottle mosaic virus (CGMMV) population in Poland and to establish the phylogenetic relationships between the Polish and other isolates described to date, 91 isolates were collected from cucumber plants. The analysis, based on coat protein (CP) gene, revealed the presence of two phylogenetic groups: one consisting of the Polish isolates collected in 2017 and those originated mainly from Asia region and the second including the Polish isolates collected in 2016 and the others from European countries. The sensitive, specific, and rapid one-step loop-mediated isothermal amplification assay was developed for the early detection of genetically diverse CGMMV isolates in seeds and plant material. Keywords: CGMMV, cucumber, genetic variability, identification, LAMP method Journal: Plant Protection Science Pages: 1-7 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/92/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/92/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0002.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:92-2018-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jaroslav Polák Author-Workplace-Name: Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic Author-Name: Tereza Neubauerová Author-Name: Petr Komínek Author-Workplace-Name: Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic Author-Name: Jiban Kumar Kundu Author-Workplace-Name: Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic Title: Reaction of transgenic plum cv. HoneySweet to the Plum pox virus after a severe infection of Monilinia sp. - short communication Abstract: Resistance to Plum pox virus (PPV) in transgenic Prunus domestica L., clone C5 (cv. HoneySweet) was evaluated in a regulated field in the Czech Republic for fifteen years (2002-2016). PPV mild symptoms appeared in C5 trees only in several leaves situated close to the point of inoculum grafting up to 2010. No symptoms of PPV were observed in the years 2011-2013 and results of ELISA and RT-PCR detection tests were negative. In the twelfth year (2013), there was a severe unusual natural attack of plum trees by Monilinia sp. This Monilinia sp. attack occurred only one time - in 2013. There was no Monilinia sp. infection in 2002-2012 and in 2014-2016. Mild PPV symptoms reappeared in several leaves of transgenic plum trees in the next two years (2014-2015) and the presence of PPV was proved by DAS-ELISA and confirmed by RT-PCR. Keywords: interaction, sharka, monilia, plum, genetic modification Journal: Plant Protection Science Pages: 8-10 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/152/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/152/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0003.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:152-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Iveta Pánková Author-Name: Václav Krejzar Author-Workplace-Name: Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic Author-Name: Radka Krejzarová Author-Workplace-Name: Division of Crop Protection and Plant Health, Crop Research Institute, Prague, Czech Republic Title: Categorisation of reactions and enumeration of bacteria in potato cultivars inoculated with the causal agent of bacterial ring rot Abstract: Variability in the responses of plants propagated from in vitro tissue cultures of 52 ware and industrial potato cultivars to different Clavibacter michiganensis subsp. sepedonicus inoculum size was tested during 2015-2017. Bacterial ring rot symptoms on plants and tubers xylem vessels were recorded for 8 weeks and the susceptibility index (SI) for individual cultivars was calculated. Based on foliage symptoms, potato cultivars were placed into three symptoms groups. The symptomless group had SIs ≤ 1, for the moderate symptom group SIs ranged from 1.01 to 2.99, and the severe symptom group had SIs ≥ 3.0. The pathogen concentrations in vascular vessels of all infected potato plants increased during the experiment regardless of the foliage symptom group. Keywords: bacterial ring rot of potato, Clavibacter michiganensis subsp. sepedonicus, industrial potato cultivar, susceptibility index, tolerance/susceptibility, ware potato cultivar Journal: Plant Protection Science Pages: 11-22 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/44/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/44/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0004.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:44-2018-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Yoshinao Aoki Author-Workplace-Name: Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan Author-Name: Nguyen Van Trung Author-Workplace-Name: Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan Author-Name: Shunji Suzuki Title: Impact of Piper betle leaf extract on grape downy mildew: effects of combining 4-allylpyrocatechol with eugenol, α-pinene or β-pinene Abstract: Methanol extract of Piper betle leaves exhibited an inhibitory effect on grape downy mildew. This extract might contain more than two compounds which have different polarities that suppress grape downy mildew. Gas chromatograph-tandem mass spectrometry analysis identified 4-allylpyrocatechol, eugenol, α-pinene, and β-pinene in the methanol extract. Neither of the compounds suppressed grape downy mildew by single treatment. On the other hand, treatment with a combination of 4-allylpyrocatechol with eugenol, α-pinene or β-pinene enhanced the inhibitory effects on grape downy mildew and perfectly suppressed it. The complex extracted from P. betle leaves may be used in organic agriculture as an alternative to chemical fungicides in viticulture. Keywords: grapevine, pinene, Plasmopara viticola Journal: Plant Protection Science Pages: 23-30 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/53/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/53/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0005.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:53-2018-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Vladimíra Nožková Author-Workplace-Name: Department of Biophysics, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Barbora Mieslerová Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Lenka Luhová Author-Workplace-Name: Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Jana Piterková Author-Workplace-Name: Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Ondřej Novák Author-Workplace-Name: Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research Author-Workplace-Name: Institute of Experimental Botany CAS, Olomouc, Czech Republic Author-Name: Martina Špundová Author-Workplace-Name: Department of Biophysics, Faculty of Science, Palacký University, Olomouc, Czech Republic Author-Name: Aleš Lebeda Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University, Olomouc, Czech Republic Title: Effect of heat-shock pre-treatment on tomato plants infected by powdery mildew fungus Abstract: The effect of plant heat-shock (HS) pre-treatment (40.5°C, 2 h) on Pseudoidium neolycopersici development in the susceptible and moderately resistant Solanum spp. genotypes was studied together with biochemical responses (endogenous concentrations of salicylic (SA), jasmonic (JA), abscisic acid (ABA), and peroxidase (POX) activity). In HS pre-treated S. lycopersicum, an acceleration of pathogen, chlorosis and necrosis development, strong SA, JA accumulation, and increased POX activity were detected. In S. chmielewskii, HS pre-treatment caused a slight suppression of pathogen development, increase in JA, ABA concentrations, and POX activity. HS accelerated and strengthened the development of symptoms and biochemical responses to the infection in the susceptible genotype in contrast to moderately resistant genotype with a robust defence response to an infection per se. Keywords: abscisic acid, jasmonic acid, peroxidase, Pseudoidium neolycopersici, salicylic acid, Solanum spp Journal: Plant Protection Science Pages: 31-42 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/24/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/24/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0006.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:24-2018-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Sen-Sung Cheng Author-Workplace-Name: Experimental Forest, National Taiwan University, Chu-Shan, Nantou, Taiwan Author-Name: Chun-Ya Lin Author-Workplace-Name: School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan Author-Name: Min-Jay Chung Author-Workplace-Name: Experimental Forest, National Taiwan University, Chu-Shan, Nantou, Taiwan Author-Name: Ying-Ju Chen Author-Workplace-Name: Division of Forest Chemistry, Taiwan Forestry Research Institute, Taipei, Taiwan Author-Name: Shang-Tzen Chang Author-Workplace-Name: School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan Title: Potential source of environmentally benign antifungal agents from Cinnamomum osmophloeum leaves against Phellinus noxius Abstract: The antifungal activity of leaf oils from different provenances of Cinnamomum osmophloeum was evaluated against Phellinus noxius and their chemical polymorphism. C. osmophloeum leaf oils of 15 provenances and their relative contents were classified into eight chemotypes, namely cinnamaldehyde, cinnamaldehyde/linalool, linalool, cinnamaldehyde/cinnamyl acetate, linalool/camphor, camphor/bornyl acetate, 1,8-cineole/p-cymene, and mixed types according to GC-MS, CA, and PCA. It was found that leaf oils of both cinnamaldehyde and cinnamaldehyde/cinnamyl acetate types had excellent inhibitory effects against P. noxius, and their IC50 values were 119.5 and 154.1 µg/ml, respectively. Furthermore, trans-cinnamaldehyde possessed the strongest antifungal activity among the constituents against P. noxius, and its IC50 values were 116.0 µg/ml. Keywords: antifungal activity, genus Cinnamomum, trans-cinnamaldehyde, leaf oil, brown root rot disease Journal: Plant Protection Science Pages: 43-53 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/28/2017-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/28/2017-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0007.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:28-2017-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Hasan Al-Khshemawee Author-Workplace-Name: School of Veterinary and Life Science, Murdoch University, Murdoch, Australia Author-Workplace-Name: College of Agriculture, Wasit University, Wasit, Iraq Author-Name: Manjree Agarwal Author-Workplace-Name: School of Veterinary and Life Science, Murdoch University, Murdoch, Australia Author-Name: Yonglin Ren Author-Workplace-Name: School of Veterinary and Life Science, Murdoch University, Murdoch, Australia Title: Stable isotope labelling of Ceratitis capitata Abstract: The use of stable isotopes to label an insect species, the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephidae) (medfly) was investigated. Labelling allows mating and life history characteristics to be investigated experimentally. 13C6-glucose was incorporated into the diet of medflies at various stages of development by adding it to larval media or providing adults with sugar water. Data was collected from egg hatching until the death of adults. The results show that stable isotopes successfully labelled medflies in laboratory conditions. There were significant differences between labelled and unlabelled treatments in terms of eggs hatching rates, larval development, pupae emergence, adult survival, and mating behaviour. Labelling during larval development, and combined labelling at the larval and adult stages, resulted in detectable values. Labelling in the larval stage had no effect on mating behaviour, but that in the adult stage did. This study demonstrates that it is possible to label adult medflies and to detect the label after mating. Keywords: 13C6-glucose, sterile insect technique (SIT), medfly, mating behaviour, life history Journal: Plant Protection Science Pages: 54-60 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/13/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/13/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0008.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:13-2018-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Junjie Liu Author-Name: Peng Li Author-Name: Liuyang Lu Author-Workplace-Name: College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, P.R. China Author-Name: Lanfen Xie Author-Workplace-Name: College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, P.R. China Author-Name: Xiling Chen Author-Name: Baizhong Zhang Title: Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua Abstract: Eight commonly used candidate reference genes, 18S ribosomal RNA (rRNA) (18S), 28S rRNA (28S), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1α), ribosomal protein L7 (RPL7), Alpha-tubulin (α-TUB), and TATA box binding protein-associated factor (TBP), were evaluated under various experimental conditions to assess their suitability in different developmental stages, tissues and herbicide treatments in Avena fatua. The results indicated the most suitable reference genes for the different experimental conditions. For developmental stages, 28S and EF1α were the optimal reference genes, both EF1α and 28S were suitable for experiments of different tissues, whereas for herbicide treatments, GAPDH and ACT were suitable for normalizations of expression data. In addition, GAPDH and EF1α were the suitable reference genes. Keywords: wild oat, expression, herbicide Journal: Plant Protection Science Pages: 61-71 Volume: 55 Issue: 1 Year: 2019 DOI: 10.17221/20/2018-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/20/2018-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-201901-0009.txt Handle: RePEc:caa:jnlpps:v:55:y:2019:i:1:id:20-2018-PPS