Template-Type: ReDIF-Article 1.0 Author-Name: Katarzyna Trzmiel Author-Workplace-Name: Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Poznań, Poland Author-Name: Wiktoria Szydło Author-Workplace-Name: Center for Advanced Technology, Adam Mickiewicz University, Poznań, Poland Author-Workplace-Name: Population Ecology Lab, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland Author-Name: Beata Hasiów-Jaroszewska Author-Workplace-Name: Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Poznań, Poland Title: Biological and molecular characterisation of the two Polish Wheat streak mosaic virus isolates and their transmission by wheat curl mites Abstract: Wheat streak mosaic virus (WSMV) is a serious and widespread pathogen in the wheat-producing areas in the USA while, in Europe, it has been considered a minor threat to cereal crops. In the past, WSMV was detected in wheat, triticale and maize plants in Poland by DAS-ELISA. Here, we present the biological and molecular characterisation of WSMV-Sze and WSMV-Sosn isolates collected from western and southern Poland and report their transmissibility by the widespread wheat curl mite (WCM) lineage MT-8. The performed bioassays revealed that the analysed WSMV isolates infect wheat, barley, triticale, rye, oat and maize, but they differ in the symptoms induced on the infected plants. Moreover, they infect Bromus hordeaceus Linnaeus, which is increasingly recognised as a virus reservoir. The full-length genome sequence of both isolates was obtained and compared with the others described to date. The phylogenetic analysis revealed that the Polish isolates are clustered with the earlier described type B isolates of WSMV from Europe and Iran. The recombination analysis revealed the presence of recombinant variants in WSMV population and indicated that the WSMV-Sosn might originated from the intra-species recombination of the WSMV-Sze and WSMV-Cz isolates. Keywords: phylogenetic relationships, recombination, virus diversity, MT-8, WSMV, WCM Journal: Plant Protection Science Pages: 171-178 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/104/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/104/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0001.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:104-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Adyatma Irawan Santosa Author-Name: Filiz Ertunc Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Ankara University, Ankara, Turkey Title: Phylogenetic and diversity analyses of Garlic common latent virus based on the TGB and CP gene sequence Abstract: Garlic common latent virus (GarCLV, Carlavirus), in co-infection, often worsens the severity of other Allium viruses on the garlic (Allium sativum Linnaeus). The nucleotide (nt) and amino acid (aa) sequences of the TGB and CP genes were analysed to get the first deep insight into the genomic variations and population structure of GarCLV. Global recombinant-free isolates were clustered into three clades in both the nt-based phylogenetic trees of TGB and CP. The clade 3 isolates shared low similarity percentages among themselves, as well as to the clade 1 and 2 isolates. Most major aa changes in the CP were observed on its 5' and 3' ends. Clade 2 obtained the lowest S, η, k, and π values for both the TGB and CP, which indicated low variations among its isolates. Both TGB and CP have undergone a negative selection, with CP being under stronger negative pressure than TGB. Neutrality tests estimated the non-significant negative values to all clades for TGB and CP, except Tajima's D for clade 2 of the TGB. The results of the Ks*, Kst*, Z*, and Snn tests suggested that all three phylogroups were divergent to each other for both TGB and CP. The high Fst on all the clade comparisons for both TGB and CP showed a large gene flow among three clades. Keywords: molecular variability, population structure, selection pressure, similarity percentages Journal: Plant Protection Science Pages: 179-187 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/149/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/149/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0002.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:149-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Mustafa Usta Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Yuzuncu Yil University, Van, Turkey Author-Name: Abdullah Güller Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Bingöl University, Bingöl, Turkey Author-Name: Hikmet Murat Sipahioglu Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Malatya Turgut Özal University, Malatya, Turkey Title: First report of 'Candidatus Phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis L.) Abstract: The natural occurrence of 'Candidatus Phytoplasma trifolii' in pear trees (Pyrus communis Linnaeus) is reported here for the first time. In 2017, a total of thirty-five pear trees, two of them exhibiting leaf rolling along the midvein, reddening, bushy appearance, and upright growth symptoms were sampled in different locations in Van province, Turkey. The total deoxyribonucleic acid was extracted from symptomatic and asymptomatic plants. The purified DNA served as a template in nested polymerase chain reaction (nested-PCR) assays, performed to amplify 16S rRNA sequences using universal primer pairs (R16mF2/R16mR1 and R16F2n/R16R2). The resulting PCR products were then cloned into a pGEM T-Easy vector and sequenced bidirectionally. The phytoplasma strain, group, and subgroup identity were determined using the in silico restriction fragment length polymorphism (RFLP) analysis of the 16S ribosomal RNA-encoding gene sequences profiling with seventeen distinct restriction enzymes. Of the thirty-five pear samples, only two yielded 1 256 bp and 1 258 bp DNA fragments and were designated as Van-Pr3 (Acc. No. MH709141) and Van-Pr4 (Acc. No. MH730561), respectively. Based on the in silico virtual RFLP pattern analysis of the 16S rRNA sequences, we confirmed the presence of 'Ca. P. trifolii' belonging to the clover proliferation group and both identified phytoplasmas were identical with the similarity coefficient of 1.00 to the reference pattern of 16Sr group VI, subgroup A (Acc. No. AY390261). Here we report that the pear tree is an alternate host of the 'Ca. P. trifolii'. Keywords: clover proliferation group, detection, new host, pear, nested-PCR, virtual RFLP, Turkey Journal: Plant Protection Science Pages: 188-195 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/163/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/163/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0003.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:163-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Renata Iličić Author-Workplace-Name: Department for Environmental and Plant Protection, Faculty of Agriculture, University of Novi Sad, Sad, Serbia Author-Name: Jelica Balaž Author-Workplace-Name: Department for Environmental and Plant Protection, Faculty of Agriculture, University of Novi Sad, Sad, Serbia Author-Name: Vladislav Ognjanov Author-Workplace-Name: Department for Environmental and Plant Protection, Faculty of Agriculture, University of Novi Sad, Sad, Serbia Author-Name: Tatjana Popović Author-Workplace-Name: Institute for Plant Protection and Environment, Belgrade, Serbia Title: Epidemiology studies of Pseudomonas syringae pathovars associated with bacterial canker on the sweet cherry in Serbia Abstract: This study was conducted to examine the epidemiological characteristics of two Pseudomonas syringae pvs. - syringae (Pss) and morsprunorum race 1 (Psm) on the sweet cherry in both field and laboratory conditions. Cycled inoculations of one-year-old branches indicated that the sweet cherry became sensitive to Psm race 1 earlier in the season (October) compared to Pss (November). The most severe infections occurred in the dormancy period (November), while the necroses formed in January and March were less in their length. Inoculations of the two-/three-year-old branches performed in November resulted in necrosis, with those induced by Pss being more aggressive. The January inoculations, however, resulted in less formed necroses in length. Laboratory tests performed on excised sweet cherry branches confirmed the results yielded by the field experiments, indicating that the dormancy period poses the greatest risk for the P. syringae pvs. infection. The isolation of Pss and Psm bacteria from naturally infected sweet cherry samples (cankers) during the summer indicated that the bacteria that persists in cankers in low numbers became active during autumn. Significant Pss and Psm race 1 epiphytic bacterial populations were noted during the spring, but decreased in the summer and increased again in the autumn, indicating the presence of inoculum reservoirs. The greater understanding of the control strategies aimed at the epidemiological factors should, thus, facilitate better disease outbreak management. Keywords: bacterial dieback, field and laboratory inoculation, natural infection, sweet cherry Journal: Plant Protection Science Pages: 196-205 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/140/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/140/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0004.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:140-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Yi-Ru Lai Author-Workplace-Name: Department of Plant Medicine, Faculty of Agriculture, National Chiayi University, Chiayi, Taiwan Author-Name: Chih-Hung Lin Author-Workplace-Name: Department of Plant Medicine, Faculty of Agriculture, National Chiayi University, Chiayi, Taiwan Author-Name: Chun-Pi Chang Author-Workplace-Name: Department of Plant Medicine, Faculty of Agriculture, National Chiayi University, Chiayi, Taiwan Author-Name: Hui-Fang Ni Author-Workplace-Name: Department of Plant Protection, Chiayi Agricultural Experiment Station, Taiwan Agricultural Research Institute, Chiayi, Taiwan Author-Name: Wen-Shi Tsai Author-Workplace-Name: Department of Plant Medicine, Faculty of Agriculture, National Chiayi University, Chiayi, Taiwan Author-Name: Chien-Jui Huang Author-Workplace-Name: Department of Plant Medicine, Faculty of Agriculture, National Chiayi University, Chiayi, Taiwan Title: Distribution of copper resistance gene variants of Xanthomonas citri subsp. citri and Xanthomonas euvesicatoria pv. perforans Abstract: In Taiwan, numerous crops are threatened by Xanthomonas diseases such as citrus bacterial canker caused by X. citri subsp. citri and tomato bacterial spot mainly caused by X. euvesicatoria pv. perforans. Foliar sprays of copper-based bactericides have been frequently used for control of plant bacterial diseases. However, in Taiwan not much attention was paid on copper-resistant (CuR) Xanthomonas spp. and their impact on disease control efficacy of copper-based bactericides. In this study, CuR Xanthomonas isolates were collected from citrus and tomato in Taiwan. Compared with the pronounced effect on the copper sensitive isolate, spraying of copper hydroxide at the recommended rate of 0.5 kg/ha could not protect tomato plants against bacterial spot caused by the CuR isolate. Phylogenetic analysis of concatenated copper resistance genes, copL, copA, and copB, indicate that the Taiwanese CuR isolates belong to the worldwide clade. In addition to the three previously reported variants of the copB gene, analysis of complete copB sequences from xanthomonads associated with citrus and solanaceous hosts revealed the other three variants of copB and their global distribution. Copper-resistant Xanthomonas isolates from Taiwan have the two unreported variants of copB genes which differ from the other three previously reported types in the sizes and structures. The information provided here reveals the necessity to develop and include alternative measures rather than relying on foliar sprays of copper bactericides for sustainable control of tomato bacterial spot in Taiwan. Keywords: citrus, efficacy, polymorphism, tomato, xanthomonad Journal: Plant Protection Science Pages: 206-216 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/160/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/160/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0005.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:160-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Meral Yilmaz Author-Workplace-Name: Department of Seed Pathology and Technology, Turkish Ministry of Agriculture and Forestry, Antalya Seed Certification and Test Agency, Antalya, Turkey Author-Name: Ömür Baysal Author-Workplace-Name: Molecular Microbiology Unit of Molecular Biology and Genetics Department, Faculty of Science, Muğla Sitki Koçman University, Muğla, Turkey Author-Name: Ragip Soner Sīlme Author-Workplace-Name: Center for Research and Practice in Biotechnology and Genetic Engineering, Istanbul University, Istanbul, Turkey Title: The effect of a seed coating with Origanum vulgare essential oil on Clavibacter michiganensis subsp. michiganensis Abstract: The study aimed to evaluate the efficacy of a seed film coating with Origanum vulgare Linnaeus essential oil (EO) against Clavibacter michiganensis subsp. michiganensis (Cmm) on tomatoes. Tomato seeds (cv. Rio Grande) coated with different doses of EO derived from O. vulgare were inoculated with Cmm (1.8 × 108 CFU/mL). O. vulgare's EO showed a remarkable inhibition effect on the Cmm growth. The EO effect against Cmm was determined based on the parameters, such as the inhibition zone and bacterial population in a seed. The GC-MS analysis of EO showed that carvacrol is the major component (at 74.05%), which may inhibit the bacterial growth. Later, we have expanded our studies to determine the inhibitory effect of the EO's mode of action on the pathogenic bacteria with a molecular docking analysis based on the molecular protein-ligand interaction. The results showed that carvacrol has a strong interaction with the bacterial expansin protein (PDB 4JJO) of Cmm and the qPCR analyses confirmed the effect of the O. vulgare treatment against Cmm. This original approach has the prominent potential to prevent seed transmission of Cmm for seed quality in the world, suggesting a method for paving the way for Cmm disease management. Keywords: bacterial canker, carvacrol, molecular docking, protein-ligand interaction, real-time PCR, seed film coating Journal: Plant Protection Science Pages: 217-225 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/177/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/177/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0006.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:177-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Xiaohua Yao Author-Workplace-Name: Qinghai University, Xining, China Author-Workplace-Name: Qinghai Academy of Agricultural and Forestry Sciences, Xining, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, China Author-Name: Yue Wang Author-Workplace-Name: Qinghai University, Xining, China Author-Name: Youhua Yao Author-Workplace-Name: Qinghai University, Xining, China Author-Workplace-Name: Qinghai Academy of Agricultural and Forestry Sciences, Xining, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, China Author-Name: Likun An Author-Workplace-Name: Qinghai University, Xining, China Author-Workplace-Name: Qinghai Academy of Agricultural and Forestry Sciences, Xining, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, China Author-Name: Yixiong Bai Author-Workplace-Name: Qinghai University, Xining, China Author-Workplace-Name: Qinghai Academy of Agricultural and Forestry Sciences, Xining, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, China Author-Name: Xin Li Author-Workplace-Name: Qinghai University, Xining, China Author-Workplace-Name: Qinghai Academy of Agricultural and Forestry Sciences, Xining, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, China Author-Name: Kunlun Wu Author-Workplace-Name: Qinghai University, Xining, China Author-Workplace-Name: Qinghai Academy of Agricultural and Forestry Sciences, Xining, China Author-Workplace-Name: Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China Author-Workplace-Name: Qinghai Subcenter of National Hulless Barley Improvement, Xining, China Author-Name: Youming Qiao Author-Workplace-Name: Qinghai University, Xining, China Title: Use of gene family analysis to discover argonaut (AGO) genes for increasing the resistance of Tibetan hull-less barley to leaf stripe disease Abstract: Leaf stripe is a common, but major infectious disease of barley, severely affecting the yield and quality. However, only a few genes have been identified by conventional gene mapping. Gene family analysis has become a fast and efficient strategy for gene discovery. Studies demonstrated that Argonaute (AGO) proteins play an important role in plant disease resistance. Thus, we obtained nine HvAGO genes via mRNA sequencing before and after a Pyrenophora graminea infection of a disease-resistant variety "Kunlun 14" and a susceptible variety "Z1141". We analysed the physicochemical characteristics, gene structures, and motifs of the HvAGO gene sequences and found that these proteins were divided into four clusters by evolutionary distance. There was high consistency in the number of exons, size, and the number and type of motifs in the different clusters. Based on protein phylogenetics, they could be divided into three branches. A collinearity analysis of Tibetan hull-less barley and Arabidopsis thaliana, rice, and maize showed that four genes were collinear with respect to the other three species. The qRT-PCR showed the expression levels of HvAGO1, HvAGO2 and HvAGO4 were significantly increased after infection with Pyrenophora graminea. These three members of the AGO gene family are, thus, speculated to play an important role in barley leaf stripe resistance. The results provide reference for the application of HvAGO genes in the leaf stripe control and the exploration of disease resistance genes in other crops. Keywords: gene expression, HvAGO1, HvAGO2, HvAGO4, Pyrenophora graminea Journal: Plant Protection Science Pages: 226-239 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/180/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/180/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0007.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:180-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Teodora Toshova Author-Workplace-Name: Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: Dimitar Velchev Author-Workplace-Name: Maize Research Institute, Agricultural Academy, Knezha, Bulgaria Author-Name: Daniela Pilarska Author-Workplace-Name: Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Workplace-Name: Department of Natural Sciences, New Bulgarian University, Sofia, Bulgaria Author-Name: Ivaylo Todorov Author-Workplace-Name: Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: Slavimira Draganova Author-Workplace-Name: Institute of Soil Science, Agrotechnologies and Plant Protection, Agricultural Academy, Sofia, Bulgaria Author-Name: Jaroslav Holuša Author-Workplace-Name: Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Prague, Czech Republic Author-Name: Danail Takov Author-Workplace-Name: Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria Title: Effect of bioinsecticides on the grey maize weevil Tanymecus dilaticollis Abstract: Tanymecus dilaticollis is an important pest of maize and sunflowers in eastern Europe. In the present study, we examined the effectiveness of two commercially available bioinsecticides against T. dilaticollis adults under laboratory conditions: Naturalis® based on the Beauveria bassiana fungus and an azadirachtin-based botanical product NeemAzal T/S®. Our results suggested that adults of the pests are more susceptible to B. bassiana than to azadirachtin. Naturalis®, tested at six concentrations (2.3 × 102 - 2.3 × 107 conidia/mL), showed a high lethal effect to T. dilaticollis adults, and 100% corrected mortality was observed in the treatments over 2.3 × 105 conidia/mL and 2.3 × 106 conidia/mL for females and males, respectively. NeemAzal T/S®, tested at four concentrations (0.5, 1, 2 and 4%) with two treatments, caused generally low to moderate mortality - 6-44% within 16 days. A field experiment consisted of two sprayings of maize experimental plots with Naturalis® at a rate of 200 mL/0.1 ha and then the recording the number of dead adults of T. dilaticollis in the treated and control plots was conducted in north-western Bulgaria. Twenty days after treatment, a significantly higher mean mortality of the pest caused by the mycoinsecticide than in the control variants was registered. Keywords: maize pest, Beauveria bassiana, azadirachtin, mortality, lethal concentration, median lethal time Journal: Plant Protection Science Pages: 240-247 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/17/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/17/2021-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0008.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:17-2021-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jozef Vakula Author-Workplace-Name: National Forest Centre, Forest Research Institute Zvolen, Forest Protection Service Centre, Banská Štiavnica, Slovakia Author-Name: Milan Zúbrik Author-Workplace-Name: National Forest Centre, Forest Research Institute Zvolen, Forest Protection Service Centre, Banská Štiavnica, Slovakia Author-Name: Juraj Galko Author-Workplace-Name: National Forest Centre, Forest Research Institute Zvolen, Forest Protection Service Centre, Banská Štiavnica, Slovakia Author-Name: Andrej Gubka Author-Workplace-Name: National Forest Centre, Forest Research Institute Zvolen, Forest Protection Service Centre, Banská Štiavnica, Slovakia Author-Name: Andrej Kunca Author-Workplace-Name: National Forest Centre, Forest Research Institute Zvolen, Forest Protection Service Centre, Banská Štiavnica, Slovakia Author-Name: Christo Nikolov Author-Workplace-Name: National Forest Centre, Forest Research Institute Zvolen, Forest Protection Service Centre, Banská Štiavnica, Slovakia Author-Name: Miroslav Saniga Author-Workplace-Name: Institute of Forest Ecology, Zvolen, Slovakia Author-Name: Peter Zach Author-Workplace-Name: Institute of Forest Ecology, Zvolen, Slovakia Title: Is the double-spined bark beetle Ips duplicatus a new threat to Picea omorika in urban habitats? Abstract: The double-spined bark beetle Ips duplicatus (Coleoptera: Curculionidae, Scolytinae) is an invasive forest pest having a broad range of coniferous hosts within Europe. We found this species to be also developing in the Serbian spruce Picea omorika. Ips duplicatus infested 14 (52%) of 27 P. omorika mature trees in an arboretum garden in northern Slovakia in Central Europe during the summer of 2019. Logs from the upper part of stems of P. omorika trees placed in eclectors in the laboratory, yielded a total of 179 individuals of three scolytine species, with prevalence of I. duplicatus. Our results show that I. duplicatus also colonized less likely host such as the Serbian spruce, causing threat to this ornamental tree and contributing to its mortality in urban habitats during hot and dry summer weather. Keywords: Serbian spruce, invasive insect, tree health, urban forestry Journal: Plant Protection Science Pages: 248-251 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/7/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/7/2021-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202103-0009.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:7-2021-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Aleš Lebeda Title: Book review Journal: Plant Protection Science Pages: 252-254 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/38/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/38/2021-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:38-2021-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Vladan Ondřej Author-Name: Ian R. Curte Author-Name: Geoffrey R. Dixon Author-Name: Jeremy J. Burdon Author-Name: Ray D. Martyn Author-Name: Larry D. Knerr Author-Name: Ir. Kees Reinink Title: Professor Aleš Lebeda at Seventy Journal: Plant Protection Science Pages: 255-262 Volume: 57 Issue: 3 Year: 2021 DOI: 10.17221/53/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/53/2021-PPS.html File-Format: text/html Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:53-2021-PPS