Template-Type: ReDIF-Article 1.0 Author-Name: Engy E. Abdel Aleem Author-Name: Mona Rabie Author-Workplace-Name: Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt Author-Name: Faiza A. Fattouh Author-Workplace-Name: Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt Title: Molecular characterisation of Zucchini yellow mosaic virus infecting Cucurbita pepo in Egypt Abstract: The complete nucleotide sequence of Zucchini yellow mosaic virus isolate from Egypt (ZYMV-Egz_MT383108) was determined. The sequence comparisons suggested that the isolate belongs to Group A. The sequence analysis of the Egyptian isolate showed the highest similarity (~96-97%) with the isolates leaf1 (KJ923767.1) and PA_2006 (JQ716413.1) from the USA and the lowest similarity (84%) with an isolate (AF014811.2) from Singapore. The phylogenetic analysis revealed that ZYMV-Egz occupied a distinct clade together with the USA isolates in Group A, known to be the most widespread throughout the world. This is a first record of the complete nucleotide sequence of an Egyptian isolate of ZYMV. Keywords: cucurbits, genome sequence, phylogenetic analysis, RT-PCR, virus identification Journal: Plant Protection Science Pages: 263-270 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/191/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/191/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0001.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:191-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ping Li Author-Workplace-Name: Department of Horticulture and Landscape, Anqing Vocational and Technical College, Anqing, P. R. China Author-Name: Dong Liu Title: Genetic diversity among asexual and sexual progenies of Phytophthora capsici detected with ISSR markers Abstract: The population structure of Phytophthora capsici among asexual and sexual progenies was analyzed using ISSR. Thirty asexual progenies of one parent and three sexual populations were assayed for genetic diversity using 5 ISSR primers and DNA from 120 offspring of P. capsici was amplified. In total, 71 reproducible ISSR fragments were obtained, of which 100% were polymorphic, revealing high polymorphism among the isolates. Among them, the percentages of polymorphism of sexual and asexual progeny isolates were 100.00 and 77.46%, respectively. Genetic similarity coefficients among all the isolates ranged from 0.54 to 0.73. The sexual offspring population showed much more variability than the asexual offspring population with 76.26% variability attributed to diversity within populations as compared with 23.74% among populations. This research reveals that the sexual progeny population of P. capsici contributes more genetic diversity than that of asexual progeny population. Keywords: asexual offspring population, ISSR-PCR, pepper phytophthora blight, sexual offspring population Journal: Plant Protection Science Pages: 271-278 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/28/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/28/2021-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0002.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:28-2021-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Alba Alvarez-Morezuelas Author-Workplace-Name: NEIKER-Basque Institute for Agricultural Research and Development-BRTA, Vitoria, Spain Author-Name: Nestor Alor Author-Workplace-Name: NEIKER-Basque Institute for Agricultural Research and Development-BRTA, Vitoria, Spain Author-Name: Leire Barandalla Author-Workplace-Name: NEIKER-Basque Institute for Agricultural Research and Development-BRTA, Vitoria, Spain Author-Name: Enrique Ritter Author-Workplace-Name: NEIKER-Basque Institute for Agricultural Research and Development-BRTA, Vitoria, Spain Author-Name: Jose Ignacio Ruiz de Galarreta Title: Virulence of Phytophthora infestans isolates from potato in Spain Abstract: The oomycete Phytophthora infestans is responsible for the disease known as late blight in potato and tomato. It is the plant pathogen that has caused the greatest impact on humankind so far and, despite all the studies that have been made, it remains the most important in this crop. In Spain during the last years a greater severity of the disease has been observed in both, potato and tomato, probably due to genetic changes in pathogen populations described recently. The aim of this study was the characterization of the physiological strains of 52 isolates of P. infestans obtained in different potato-growing areas in Spain. For this purpose, inoculations on detached leaves were performed in order to determine compatibility or incompatibility reactions. A total of 17 physiological races were found. The less frequent virulence factors were Avr5 and Avr8. By studying the epidemiology of the pathogen, a specific breeding program for late blight resistance can be implemented. Keywords: physiological strains, late blight, Solanum tuberosum, virulence Journal: Plant Protection Science Pages: 279-288 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/39/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/39/2021-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0003.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:39-2021-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Caiyun Liu Author-Workplace-Name: Key Laboratory of Biochemistry and Molecular Biology in University of Shandong Province, Biological and Agricultural College, Weifang University, Weifang, China Author-Name: Jing Zhao Author-Workplace-Name: Key Laboratory of Biochemistry and Molecular Biology in University of Shandong Province, Biological and Agricultural College, Weifang University, Weifang, China Author-Name: Hui Cao Title: Study on the antifungal effect and mycolytic activity of the biocontrol agent Chaetomium subaffine LB-1 Abstract: The antifungal effect and mucolytic activity of a newly screened biocontrol strain Chaetomium subaffine LB-1 were researched in this study. The results found that LB-1 has good antifungal effects on the test plant pathogenic fungi Botrytis cinerea Pers. ex Fr., Fusarium oxysporum f. sp. cucumerinum Owen and Alternaria solani (Ellis & Martin) Sorauer in dual culture assay, with the inhibition rates of 61.39, 50.34 and 51.22%, respectively. Flocculated and dissolved hyphae of the phytopathogenic fungi were observed at the interaction zone on a dual-cultured PDA plate, but the hyphae of LB-1 were normal. The LB-1 cell-free filtrate has significant inhibitory effects on the three tested fungi in the poison plate assay; dissolved colonies, vesiculated and flocculated hyphae of the test pathogenic fungi were also found on the PDA plates supplemented with the LB-1 cell-free filtrate. Clear halo zones around the LB-1 colonies were found on the protease test plate, pectinase test plate and cellulose test plate, indicating that LB-1 could produce mucolytic enzymes of protease, pectinase and cellulase. However, the activities of chitinase and β-l,3-glucase were not detected on their test plates from LB-1. An obvious oil-displaced circle was formed in the oil spreading test, indicating that a surface-active substance might be contained in the LB-1 cell-free filtrate. These results proved that the biocontrol agent of C. subaffine LB-1 could exert its antifungal effects via living competition and mycolysis, and the latter may be obtained by production of mycolytic enzymes and surface-active substances. . Keywords: biocontrol strain LB-1, cell-free filtrate, hyphae dissolution, living competition, mycolytic enzyme, surface-active substance Journal: Plant Protection Science Pages: 289-296 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/65/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/65/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0004.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:65-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Dahou Moutassem Author-Workplace-Name: Laboratory of Characterization and Valorization of Natural Resources, Faculty of Nature and Life Sciences, Mohamed El Bachir El Ibrahimi University, Bordj Bou Arreridj, Algeria Author-Name: Yuva Bellik Author-Workplace-Name: Laboratory of Characterization and Valorization of Natural Resources, Faculty of Nature and Life Sciences, Mohamed El Bachir El Ibrahimi University, Bordj Bou Arreridj, Algeria Author-Name: Mohamed El Hadi Sannef Author-Workplace-Name: National High School of Computer Science, Algiers, Algeria Title: Toxicity and repellent activities of Thymus pallescens and Cymbopogon citratus essential oils against Sitophylus granarius Abstract: This study evaluated the toxicity and repellent activities of essential oils (EOs) against Sitophilus granarius (Linnaeus, 1758), both in vitro and in vivo conditions. The EOs obtained from Thymus pallescens Noë. (Lamiaceae) and Cymbopogon citratus Stapf. (Poaceae) were analyzed by GC-MS, and revealed that carvacrol (56.64%) and geraniol (20.8%) as the respective major components. T. pallescens EO was found to be a more effective toxicant, with LC50 and LC90 values of 9.3 and 34.6 µL/mL, respectively, in the contact test vs 8.2 and 25.3 µL/mL in the fumigation test. T. pallescens EO showed also a stronger repellent effect with values ranging from 83.4% to 100%. In the in vivo test, the survival probability decreased from 99.9 to 2.48% among insects exposed to T. pallescens EO. These data demonstrated that EOs could be considered effective alternatives to chemical insecticides, providing pest control for stored products in an ecologically sustainable manner. Keywords: contact toxicity, corrected mortality, fumigation toxicity, metabolic profile, survival probability Journal: Plant Protection Science Pages: 297-309 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/185/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/185/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0005.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:185-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Serdal Bozkurt Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, University of Van Yüzüncü Yil Üniversitesi, Van, Turkey Author-Name: Evin Polat Akköprü Title: Effects of five different melon (Cucumis melo L.) cultivars on population growth of Aphis gossypii (Glover) Abstract: In this study, the population growth parameters of Aphis gossypii Glover (Hemiptera: Aphididae) on five different melons [Cucumis melo Linnaeus (Cucurbitaceae) cultivars (Balin, Çitirex, 221 F1, Gediz, Yusufbey)], which are commonly grown in Turkey, were investigated in laboratory experiments. Life table parameters were estimated according to age-stage and two-sex life table theory. The results showed that A. gossypii developed more successfully on the Gediz cultivar due to the higher intrinsic rate of increase (0.5042 1/day), the finite rate of increase (1.6557 1/day), and the shorter mean generation time (8.161 days) than the other cultivars. Ecological pest control against aphids' use of resistance or less favorable host plant cultivars is considered one of the significant components of pest management. The findings obtained in this study can be used in the pest management program designed for the aphid. Keywords: intrinsic rate of increase, life table, melon aphid, population growth simulation, population parameters Journal: Plant Protection Science Pages: 310-317 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/55/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/55/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0006.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:55-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Mohamed Elimem Author-Workplace-Name: Research Laboratory of Agriculural Production Systems and Sustainable Development, High School of Agriculture of Mograne, University of Carthage, Zaghouane, Tunisia Author-Name: Ahlem Harbi Author-Workplace-Name: Higher Institute of Agronomy of Chott-Mériem, University of Sousse, Chott-Mériem, Tunisia Author-Name: Essia Limem-Sellemi Author-Workplace-Name: General Directorate of Agricultural Protection, Ministry of Agriculture of Water Resources and Fisheries, Tunis le Belvedere, Tunisia Author-Name: Slim Rouz Author-Workplace-Name: Research Laboratory of Agriculural Production Systems and Sustainable Development, High School of Agriculture of Mograne, University of Carthage, Zaghouane, Tunisia Author-Name: Karima Chahed Author-Workplace-Name: Research Laboratory of Agriculural Production Systems and Sustainable Development, High School of Agriculture of Mograne, University of Carthage, Zaghouane, Tunisia Author-Name: Mohamed Amine Bouchkara Author-Workplace-Name: Research Laboratory of Agriculural Production Systems and Sustainable Development, High School of Agriculture of Mograne, University of Carthage, Zaghouane, Tunisia Author-Name: Brahim Chermiti Author-Workplace-Name: Higher Institute of Agronomy of Chott-Mériem, University of Sousse, Chott-Mériem, Tunisia Author-Name: Adel Jammezi Author-Workplace-Name: General Directorate of Agricultural Protection, Ministry of Agriculture of Water Resources and Fisheries, Tunis le Belvedere, Tunisia Title: Improving Ceratitis capitata control through the mass trapping technique in an IPM programme on apricots in Tunisia Abstract: New techniques for Ceratitis capitata (Wiedemann, 1824) control are being studied and developed to replace traditional organophosphate pesticide applications. A mass trapping strategy offers promising medfly control within integrated pest management (IPM) programmes. Field assays were performed to study the efficacy of two mass trapping techniques based on PheroNorm® and Ceratrap® lures that were compared to a conventional approach to control the medfly in Tunisian apricot orchards. The results showed that both mass trapping techniques had a similar efficiency in reducing the C. capitata population. The degrees Brix in the fruits was a determinant issue since the initial apricot fruit damage was detected at 6.4%. At harvesting, the lowest fruit damage rate was recorded in the PheroNorm® (4.25%) and Ceratrap® (6.50%) treated orchards, compared with the conventional approach (10.75%). Therefore, the use of 50 PheroNorm® and Ceratrap® traps per ha density within an IPM approach may be very useful to control the C. capitata populations in apricot orchards. Keywords: conventional approach, Ceratrap®, , degrees Brix, medfly, PheroNorm®, Prunus armeniaca Journal: Plant Protection Science Pages: 318-325 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/13/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/13/2021-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0007.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:13-2021-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Dragana Šunjka Author-Workplace-Name: Department for Plant and Environmental Protection, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Sanja Lazić Author-Workplace-Name: Department for Plant and Environmental Protection, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Slavica Vuković Author-Workplace-Name: Department for Plant and Environmental Protection, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Aleksandra Alavanja Author-Workplace-Name: Department for Plant and Environmental Protection, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Đura Nađ Author-Workplace-Name: Department for Plant and Environmental Protection, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia Author-Name: Siniša Mitrić Author-Workplace-Name: Faculty of Agriculture, University of Banja Luka, Banja Luka, Bosnia and Herzegovina Title: Residue and dissipation dynamic of spinetoram insecticide in pear fruits Abstract: Cacopsylla pyri (Linnaeus, 1758) is the most significant and widespread pear pest. It attacks the pear only, causing direct and indirect damages. This study was conducted in order to evaluate the efficacy of insecticide spinetoram in the control of C. pyri. The experiment was set up according to EPPO methods, at the locality Kula (Republic of Serbia) in a pear orchard (Williams variety). Spinetoram (250 g/kg a.i., WG) was foliar applied in the amount of 0.3 kg/ha, during overlapping pest generations, when pear was on BBCH 75 scale. The efficacy was evaluated through the number of larvae aged L1-L3 and L4-L5. Spinetoram has shown satisfying efficacy, especially in the control of L1-L3 larvae (81.6%). In a dissipation study, spinetoram residues in pear fruits were determined using QuEChERS method followed by HPLC-DAD. The method was validated to fulfill SANTE/12682/2019 criteria. Three days after the application, spinetoram residues in pear fruits were below the MRL (0.2 mg/kg). The results have shown that spinetoram dissipation pattern followed the first-order kinetics (R2 = 0.979) with a half-life of 2.17 days, in pear fruits. This study suggests that spinetoram could be safely used in pear, and it could take an important place in insecticide resistance management. Keywords: spinosyne, pear psylla, DT50, pre-harvest interval, PHI Journal: Plant Protection Science Pages: 326-332 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/154/2020-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/154/2020-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0008.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:154-2020-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Hassouna Bahrouni Author-Workplace-Name: National Research Institute for Rural Engineering, Water and Forestry, University of Carthage, Ariana, Tunisia Author-Name: Hanene Chaabane Author-Workplace-Name: National Agronomic Institute of Tunisia, Tunis, Tunisia Author-Name: Nidhal Marzougui Author-Workplace-Name: National Research Institute for Rural Engineering, Water and Forestry, University of Carthage, Ariana, Tunisia Author-Name: Sana Ben Meriem Author-Workplace-Name: National Research Institute for Rural Engineering, Water and Forestry, University of Carthage, Ariana, Tunisia Author-Name: Houcine Bchini Author-Workplace-Name: National Institute for Agricultural Research of Tunisia, University of Carthage, Tunis, Tunisia Author-Name: Mohamed Ali Ben Abdallah Author-Workplace-Name: National Research Institute for Rural Engineering, Water and Forestry, University of Carthage, Ariana, Tunisia Title: Effect of sprayer parameters and wind speed on spray retention and soil deposits of pesticides: Case of artichoke cultivar Abstract: Irrational use of chemical method for crop protection, presents increasingly serious risks for human health and the environment. Droplet size and meteorological parameters are key factors to both environmental contamination and pest control efficacy. The objective of this study is to assess the impact of the nozzle use parameters, the operating pressure and the wind speed on droplet foliage deposition (retention) and soil deposition (losses), when treating artichoke. Several combinations were tested in a wind tunnel and in the field, under Mediterranean microclimatic conditions, using a fluorescent dye as a substitute for pesticide. Multiple regression models were built from tunnel data to predict foliage deposition and soil deposits, with determination coefficients of 0.96. Thus, models are able to simulate pesticide deposition on artichoke leaves and soil deposition, depending on sprayer parameters and wind speed. Foliage deposition and soil deposits rates ranged from 30 to 52% and 26 to 57% respectively for anti-drift nozzle. For conventional nozzle, rates varied from 20 to 38% and 31 to 62%. To improve retention and reduce spray losses, it is recommended to choose a medium droplet size when using an anti-drift nozzle, in conjunction with medium nozzle size, medium pressure and reduced wind speed. Keywords: droplets deposition, droplet size, meteorological factors, nozzle size, nozzle type, pressure Journal: Plant Protection Science Pages: 333-343 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/29/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/29/2021-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0009.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:29-2021-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Jana Tomašechová Author-Workplace-Name: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic Author-Workplace-Name: Biomedical Research Center of the Slovak Academy of Sciences, Institute of Virology, Bratislava, Slovak Republic Author-Name: Lukáš Predajňa Author-Workplace-Name: Biomedical Research Center of the Slovak Academy of Sciences, Institute of Virology, Bratislava, Slovak Republic Author-Name: Daniel Mihálik Author-Workplace-Name: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic Author-Name: Michaela Mrkvová Author-Workplace-Name: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic Author-Name: Pavel Cejnar Author-Workplace-Name: Department of Computing and Control Engineering, Faculty of Chemical Engineering, University of Chemistry and Technology in Prague, Prague, Czech Republic Author-Name: Katarína Šoltys Author-Workplace-Name: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic Author-Name: Sead Sabanadzovic Author-Workplace-Name: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, USA Author-Name: Miroslav Glasa Author-Workplace-Name: Department of Biology and Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius, Trnava, Slovak Republic Author-Workplace-Name: Biomedical Research Center of the Slovak Academy of Sciences, Institute of Virology, Bratislava, Slovak Republic Title: Characterization of an isolate of Lettuce big-vein associated virus (LBVaV) detected in naturally infected tomato (Solanum lycopersicum L.) in Slovakia Abstract: A tomato plant (Solanum lycopersicum Linnaeus, labelled KVE) displaying virus-like symptoms, tested negative for common tomato viruses, was subjected to high-throughput sequencing (HTS) on the Illumina MiSeq platform using ribosomal RNA-depleted total RNA as a template. The analysis has revealed the contigs mapping to Lettuce big-vein associated virus (LBVaV). The near complete LBVaV-KVE sequence of RNA1 and RNA2 revealed 95.0 and 94.9% identity with the reference sequence, the same length of translated products and a typical varicosavirus genome organisation. After initial long-term maintenance of LBVaV-KVE in the original plant, the virus could be detected by RT-PCR or nanoLC-ESI-Q-TOF in new plants generated from lateral shoot cuttings or inoculated by stem chips, although not uniformly. So far, LBVaV was reported to infect lettuce and related species. Our study expands the natural host range of the LBVaV to tomato. Keywords: genome, next generation sequencing, Solanaceae, varicosavirus Journal: Plant Protection Science Pages: 344-348 Volume: 57 Issue: 4 Year: 2021 DOI: 10.17221/56/2021-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/56/2021-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202104-0010.txt Handle: RePEc:caa:jnlpps:v:57:y:2021:i:4:id:56-2021-PPS