Template-Type: ReDIF-Article 1.0 Author-Name: Dana Šafářová Author-Workplace-Name: Department of Cell Biology and Genetic, Faculty of Science, Palacký University Olomouc, Czech Republic Author-Name: Luboš Majeský Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University Olomouc, Czech Republic Author-Name: Milan Navrátil Author-Workplace-Name: Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Czech Republic Title: Molecular study of turnip mosaic virus population in the Czech Republic Abstract: Turnip mosaic virus (TuMV) is the most important virus of brassica crops. In our study, we compare the genetic structure of two Czech TuMV populations sampled in the country's 25-year interval of virus presence. The 21 isolates, mainly infecting rutabaga and horseradish, were collected from four farms under organic production, and nearly complete genome sequences, 9 596-9 787 nt in length, were obtained using Sanger sequencing for all of them. The analysis of variability and polymorphism showed differences in genetic structure but the relative stability of both populations and moderate negative selection as a factor affecting the current TuMV population. The newly collected isolates are characterised by a relatively high frequency of intralineage recombinants; interlineage recombinants were not detected compared to the 25-year-old population. The phylogenetic analysis allowed the classification of all Czech isolates into world-B phylogroup, with the prevalence of isolates of subgroup B2. The spread of isolates belonging to the other phylogenetic groups posing higher phytopathological risk, which were present in the old population and some surrounding countries, was not found. Keywords: sanger sequencing, recombination, phylogeny, population changes, selection pressure Journal: Plant Protection Science Pages: 209-216 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/34/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/34/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0001.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:34-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Sokichi Shiro Author-Name: Ryu Makihara Author-Workplace-Name: Major in Agricultural and Life Sciences, Graduate School of Natural Science and Technology, Shimane University, Shimane, Japan Author-Name: Masahiro Yamaguchi Author-Workplace-Name: Department of Agricultural and Forest Sciences, Faculty of Life and Environmental Sciences, Shimane University, Shimane, Japan Author-Name: Masayuki Kadowaki Author-Workplace-Name: Institute of Agricultural and Life Sciences, Academic assembly, Shimane University, Shimane, Japan Author-Name: Yuichi Saeki Author-Workplace-Name: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan Title: Compatibility of adzuki bean (Vigna angularis) and Bradyrhizobium USDA strains, and geographical distribution and community structure on indigenous adzuki bean-nodulating bradyrhizobia in Japan Abstract: We investigated the compatibility between adzuki bean and Bradyrhizobium USDA strains and the relation between the genetic diversity of indigenous adzuki bean-nodulating bradyrhizobia and their geographical distribution in Japan. Six Bradyrhizobium USDA strains were used in the inoculation test, and B. elkanii USDA 94 showed higher symbiotic efficiency than other strains. Two adzuki bean cultivars were used to isolate indigenous adzuki bean-nodulating bradyrhizobia. Their genetic diversity and community structure were analyzed by restriction fragment length polymorphisms of PCR amplicons to target the 16S-23S rRNA gene internal transcribed spacer region, using 11 USDA Bradyrhizobium strains as reference strains. Furthermore, we performed diversity analysis, non-metric multidimensional scaling analysis based on the Chao index, and polar ordination analysis to explain the relation between community structure and geographical distribution of the adzuki bean-nodulating bradyrhizobia. The major clusters were Bj123 in Hokkaido and Kyoto, Bd110 in Fukushima and Shimane A, Be76 in Shimane B and Fukuoka, Bj6 in Miyazaki, and Bj6 and BeOK in Okinawa. Japan's indigenous adzuki bean-nodulating bradyrhizobial community was strongly and significantly correlated with latitude, longitude, annual mean temperature, and electrical conductivity. This result suggests that the adzuki bean-nodulating bradyrhizobial community is influenced by multiple environmental factors. Keywords: Bradyrhizobium, adzuki bean, compatibility, genetic diversity, environmental factors Journal: Plant Protection Science Pages: 217-232 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/64/2022-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/64/2022-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0002.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:64-2022-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Salma Tissaoui Author-Workplace-Name: Laboratory of Bioggressors and Integrated Pest Management in Agriculture (LR14AGR02), National Agronomic Institute of Tunisia, University of Carthage Tunis, Republic of Tunisia Author-Name: Noura Omri-Benyoussef Author-Workplace-Name: Crop Improvement Laboratory, National Institute of Agronomy of Tunisia, Ariana, Republic of Tunisia Author-Name: Hatem Chaar Author-Name: Marwa Hassine Author-Workplace-Name: Laboratory of Genetics and Cereal Breeding, National Agronomic Institute of Tunisia, University of Carthage, Tunis, Republic of Tunisia Author-Name: Jean-Stephane Venisse Author-Workplace-Name: Clermont Auvergne University, INRAE, Clermont-Ferrand, France Author-Name: Bouzid Nasraoui Author-Workplace-Name: Laboratory of Bioggressors and Integrated Pest Management in Agriculture (LR14AGR02), National Agronomic Institute of Tunisia, University of Carthage Tunis, Republic of Tunisia Author-Name: Amira Mougou-Hamdane Author-Workplace-Name: Laboratory of Bioggressors and Integrated Pest Management in Agriculture (LR14AGR02), National Agronomic Institute of Tunisia, University of Carthage Tunis, Republic of Tunisia Title: Progression of wheat Tan spot under different bioclimatic stages and agricultural practices Abstract: Tan spot, caused by Pyrenophora tritici-repentis, is a severe worldwide wheat foliar disease and has become common in Tunisia. Two hundred wheat field surveys were conducted in northern Tunisia during the 2017-2019 seasons to determine disease intensity and the correlation of the tan spot with agricultural practices and environmental factors. The disease infested 67% of the visited regions. The highest prevalence (87.5%), incidence (> 30%), and severity (> 10%) were observed in sub-humid regions, contrary to the middle semi-arid regions. Logistic regression was used to analyse the correlation of incidence and severity with various factors as independent variables. In a multiple variable model, the bioclimatic stages, variety, previous crop, humidity, and rainfall were significantly associated with tan spot incidence and severity. High incidence and severity were significantly correlated to the sub-humid regions, varieties Maâli, Razzak, Karim, and Carioca, rainfall, high level of humidity, and monoculture. The correlation between the tan spot and several environmental factors must be considered to ensure successful and sustainable disease management strategies. Keywords: correlation, fields wheat, incidence, severity, sub-humid, previous crop, variety Journal: Plant Protection Science Pages: 233-244 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/103/2022-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/103/2022-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0003.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:103-2022-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Riska Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Author-Name: Jumjunidang Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Author-Name: Tri Budiyanti Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Author-Name: Eko Darma Husada Author-Workplace-Name: Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Author-Name: Ni Luh Putu Indriyani Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Author-Name: Sri Hadiati Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Author-Name: Irwan Muas Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Author-Name: Ellina Mansyah Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia Title: Stem canker of dragon fruit (Hylocereus polyrhizus): Neocytalidium sp. is the new cause of the disease and its control using the sodium salt Abstract: This study aimed to isolate and characterise a fungal pathogen associated with stem canker on dragon fruit and to evaluate the efficacy of sodium salt as an alternative control against it. The fungal pathogen was isolated and identified by morphological and cultural methods. SMNND11 and ARPN11 isolates, which are morphologically alike Neoscytalidium sp., were selected for the present study. The colony's mycelial mass was greyish-white and turned to dark greenish-grey. The shape of the arthroconidia was ellipsoid to ovoid and hyaline to dark brown with septate arthrospores. The hyphae were brown in colour, septate, branched and constricted into spore chains before disarticulation. Based on the blast analysis using the aligned sequences of the internal transcribed spacer, the SMNND11 isolate was highly genetically identical (100%) to Neoscytalidium dimidiatum, the ARPN10 isolate was 98.6% identical to Neoscytalidium sp. and the neighbour-joining analysis revealed that both isolates were grouped into the same genus, Neoscytalidium sp. The in vitro study revealed that sodium salt at a concentration of > 3% showed high potential in suppressing the mycelial growth of the SMNND11 isolates. In a field trial, a sodium salt solution at 30 g/L applied twice a week, along with rotating chemical fungicides applied once a week, were able to reduce the disease severity of the stem canker disease on the dragon fruit. This study revealed that Neoscytalidium sp., as well as N. dimidiatum, is the positive pathogen that infects dragon fruit plants in IP2TP Sumani and Aripan West Sumatra. Thus, the culture and field studies support the potential control technique to alternating chemical fungicide on dragon fruit. Keywords: pitaya, Neoscytalidium sp., canker disease Journal: Plant Protection Science Pages: 245-255 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/112/2022-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/112/2022-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0004.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:112-2022-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Stanislav Ježek Author-Workplace-Name: Plant Breeding Station Stupice, Selgen, a.s., Stupice, Czech Republic Author-Name: Pavel Horčička Author-Workplace-Name: Plant Breeding Station Stupice, Selgen, a.s., Stupice, Czech Republic Author-Name: Eva Jozová Author-Workplace-Name: Department of Genetics and Biotechnology, Faculty of Agriculture and Technology, University of South Bohemia, České Budějovice, Czech Republic Author-Name: Vladislav Čurn Author-Workplace-Name: Department of Genetics and Biotechnology, Faculty of Agriculture and Technology, University of South Bohemia, České Budějovice, Czech Republic Title: Comparison of the effect of additives during gliding arc plasma treatment on the germination of bunt spores and growth characteristics of wheat Abstract: The gliding arc cold plasma treatment of wheat seeds is an alternative to chemical seed dressing, but this treatment is not very effective. This study was focused on testing the addition of salt, water and nitrogenous compounds during plasma treatment to increase the efficiency of the plasma seed treatment. The additives were not supplied in the gaseous state, as usual, but in an aqueous solution by spraying under a plasma nozzle during the treatment. The phytosanitary effect of the plasma treatment was evaluated based on the germination of Tilletia caries spores from artificially infected seeds on water agar. The viability of the seeds, growth and yield characteristics of the plasma-treated seeds were evaluated in the laboratory, greenhouse and even in field experiments with the primary goal of reducing spore vitality. The germination of T. caries spores was mostly limited to the variant with the addition of water during plasma treatment, where spore germination reached only 32.7% of the control variant while maintaining sufficient growth properties. The experiment demonstrated the suitability of using the addition of various substances during plasma treatment in the form of aqueous solutions and increasing the effectiveness of this treatment. Keywords: cold plasma, gliding arc, physical seed treatment, Tilletia spores, seed emergence, plant development and yield Journal: Plant Protection Science Pages: 256-263 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/51/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/51/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0005.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:51-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Luka Batistič Author-Workplace-Name: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: Tanja Bohinc Author-Workplace-Name: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: Stanislav Trdan Author-Workplace-Name: Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia Title: Seasonal dynamics and abundance of brown marmorated stink bug Halyomorpha halys (Stål) on four trap crops Abstract: The main purpose of the study was to examine the potential of crops, such as soybeans (Glycine max), sunflowers (Helianthus annuus), alfalfa (Medicago sativa), and sorghum (Sorghum bicolor), as trap crops for Halyomorpha halys. We monitored the abundance and seasonal dynamics of H. halys (egg clusters, larvae, and adults) on a selected number of various crops at 10-day intervals. The experiment was performed in two different regions of Slovenia (western and central) and in two different years (2021, 2022). The results varied depending on the location and the year. In the first experiment, data from the entire year showed that sorghum was the most attractive for H. halys adults (1.56 ± 0.12), followed by sunflowers (0.61 ± 0.05), soybeans (0.37 ± 0.03) and alfalfa (0.41 ± 0.21). In the second experiment, the abundance numbers of the pest were significantly lower compared to those of the first experiment [sorghum and soybeans (0.003 3 ± 0.002), sunflowers (0.000 3 ± 0.000 3)]. In both cases, the abundance numbers increased when all the crops entered the fruit development stage (BBCH 70+). Overall, the lower density of the H. halys population and the experimental design could also be some of the factors for such a low incidence of stink bugs in the central region of the country. More research will be needed to further develop effective control methods for this invasive pest. Keywords: alfalfa, brown marmorated stink bug, sorghum, soybean, sunflower, trap crops Journal: Plant Protection Science Pages: 264-277 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/2/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/2/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0006.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:2-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Carlos I. Cortés-Martínez Author-Workplace-Name: Tecnológico Nacional de México, Instituto Tecnológico del Valle de Etla, Oaxaca, México Author-Name: Adriana Ines Rodríguez-Hernández Author-Workplace-Name: Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma de Hidalgo, Hidalgo, México Author-Name: Ma del Rocío López-Cuellar Author-Workplace-Name: Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma de Hidalgo, Hidalgo, México Author-Name: Norberto Chavarría-Hernández Author-Workplace-Name: Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma de Hidalgo, Hidalgo, México Author-Name: Rodolfo De los Santos Romero Author-Workplace-Name: Tecnológico Nacional de México, Instituto Tecnológico del Valle de Oaxaca, Oaxaca, México Title: Culture of Steinernema glaseri on three solid media and their virulence against Galleria mellonella larvae Abstract: Steinernema glaseri is a potential biocontrol agent against white grubs of the Phyllophaga spp. complex; however, its suitability for in vitro multiplication has been scarcely investigated. In this study, the effects of the surface culture of NJ-43 strain with its symbiotic bacteria Xenorhabdus poinarii on egg-yolk agar (P2), chicken-liver agar and nutrient meat-peptone (MP) agar on infective juvenile (IJ) productivity and their virulence against Galleria mellonella larvae were investigated. The bacteria on the surface of the agar were first incubated in darkness at 30 °C for 54-102 h, and then 100 surface-sterilised IJs were added. After two harvests, the accumulated productivity was higher on chicken-liver agar (536 × 103 IJs/m2 day) and P2 agar (534 × 103 IJs/m2 day) than on nutrient MP agar (58 × 103 IJs/m2 day). The mean virulence of the in vitro produced IJs was 46-60% and showed no statistically significant difference among the three culture media. In conclusion, the maximum multiplication factor of S. glaseri NJ-43 on solid media was 385, and its original virulence was retained. Keywords: biocontrol, entomopathogenic nematodes, in-vitro production, one-on-one assay Journal: Plant Protection Science Pages: 278-283 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/1/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/1/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0007.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:1-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Milan Brankov Author-Workplace-Name: Maize Research Institute "Zemun Polje", Belgrade, Serbia Author-Name: Guilherme Sousa Alves Author-Workplace-Name: University of Nebraska-Lincoln, North Platte, Nebraska, USA Author-Name: Bruno Canella Vieira Author-Workplace-Name: University of Nebraska-Lincoln, North Platte, Nebraska, USA Author-Name: Milos Zaric Author-Workplace-Name: University of Nebraska-Lincoln, North Platte, Nebraska, USA Author-Name: Greg Robert Kruger Author-Workplace-Name: University of Nebraska-Lincoln, North Platte, Nebraska, USA Title: Particle drift potential of mesotrione and rimsulfuron plus thifensulfuron-methyl tank mixture in a low-speed wind tunnel Abstract: Particle drift happens during herbicide application when droplets travel outside the intended site. Different nozzles produce various range of droplets, so they play a very important role in coverage and drift occasions. When nozzles produce small droplets, the potential for off-target movement is very high. Another important factor determining particle drift is the distance between crops. Wind velocity gives the energy to herbicide particles to move away from the target place. Therefore, a drift simulation of herbicide (mesotrione and rimsulfuron plus thifensulfuron-methyl mixture) was done in a wind tunnel, using different nozzles Extended Range (XR) and Turbo TeeJet Induction (TTI). The wind speed was set at 4.4 m/s, representing the least favourable conditions where applications are possible. In the wind tunnel, eight crops (cantaloupe, cotton, green bean, pumpkin, soybean, sunflower, wheat, and watermelon) were positioned at 4, 6, 9, and 12 m downwind distances from the nozzle, and drift was simulated. Following treatments, plants were returned to a greenhouse for 28 days, and biomass reduction was recorded. Artificial collectors (Mylar cards) and water sensitive cards were positioned alongside plants. According to obtained results, spraying with XR nozzle influences higher injuries than TTI nozzle. Tracer deposition was higher at all distances when XR nozzle was used. Accordingly, droplet numbers, covered area, Volume Median Diameter (VMD), and deposition were higher on water sensitive cards when spraying were done using XR nozzle. As a consequence, higher biomass reduction occurred using the XR nozzle. The most sensitive crops were cantaloupe, pumpkin and sunflower, while the most tolerant were soybean and wheat. Keywords: off-target movement of application, crop injuries, deposition Journal: Plant Protection Science Pages: 284-291 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/39/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/39/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0008.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:39-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Adam Hruška Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Author-Name: Pavel Hamouz Author-Workplace-Name: Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic Title: Verification of a machine learning model for weed detection in maize (Zea mays) using infrared imaging Abstract: The potential of the framework of precision agriculture points towards the emergence of site-specific weed control. In light of the phenomena, the search for a cost-effective approach can help the discipline to accelerate the practical implementation. The paper presents a near-infrared data-driven machine learning model for real-time weed detection in wide-row cultivated maize (Zea mays) fields. The basis of the model is a dataset of 5 120 objects including 18 species of weeds significant in the context of wide-row crop production in the Czech Republic. The custom model was subsequently compared with a state-of-the-art machine learning tool You only look once (version 3). The custom model achieved 94.5 % identification accuracy while highlighting the practical limitations of the dataset. Keywords: computer vision, NIR images, machine learning, visual analysis, neural networks Journal: Plant Protection Science Pages: 292-297 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/131/2022-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/131/2022-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0009.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:131-2022-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Rini Murtiningsih Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Tonny Koestoni Moekasan Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Laksminiwati Prabaningrum Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Asma Sembiring Author-Workplace-Name: Research Center for Behavioral and Circular Economics, National Research and Innovation Agency, Gedung B.J. Habibie, Jakarta, Indonesia Author-Name: Wiwin Setiawati Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Ahsol Hasyim Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Bagus Kukuh Udiarto Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Ineu Sulastrini Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Neni Gunaeni Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Eli Korlina Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Nikardi Gunadi Author-Workplace-Name: Research Center for Horticulture and Estate Crops, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia Author-Name: Harmanto Author-Workplace-Name: Indonesian Polytechnic of Agricultural Engineering, Banten, Indonesia Author-Name: Liferdi Lukman Author-Workplace-Name: Directorate General of Horticulture, Ministry of Agriculture, Jakarta, Indonesia Title: Determining the technical and economic feasibility of combining pest control techniques in open field and netting house chili cultivation systems Abstract: As one of the most widely cultivated vegetable crops worldwide, chillies (Capsicum annuum L.) face serious pest-related problems when grown in either open or protected cultivation systems. However, studies aimed at understanding the implementation of various integrated pest management (IPM) techniques on chilli production in both cultivation systems are scarce. The study aimed to evaluate the impact of implementing an integrated approach that combines the use of a sweet corn barrier, thiamethoxam, control threshold, silver plastic mulch, pest traps, and fungicides on chilli pests and diseases in open and protected farming systems. Those techniques were applied simultaneously to overcome major chilli pests and diseases that commonly co-occur in the field. The experiment conducted in West Java, Indonesia from May to November 2021 revealed that control techniques could maintain chilli pest population below their control threshold and prevented disease outbreak in both cultivation systems. The average yields of chilli cultivated in the netting house were higher than those cultivated in the open field, i.e. 20.92 t/ha and 9.77 t/ha, respectively. Furthermore, the profitability of chilli cultivation using IPM technologies has been demonstrated in both open and protected fields, with the profit generated in the protected system being 3.9 times higher than that of the open field. Keywords: barrier crops, control threshold, mulch, R/C, traps Journal: Plant Protection Science Pages: 298-302 Volume: 59 Issue: 3 Year: 2023 DOI: 10.17221/5/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/5/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202303-0010.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:3:id:5-2023-PPS