Template-Type: ReDIF-Article 1.0 Author-Name: Adyatma Irawan Santosa Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Sleman, Indonesia Author-Name: Susamto Somowiyarjo Author-Workplace-Name: Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Sleman, Indonesia Title: Ageratum yellow vein alphasatellite and tomato leaf curl Java betasatellite association with begomoviruses infecting crops and weeds in Indonesia Abstract: Symptomatic samples were taken from five districts in Yogyakarta and Central Java provinces of Indonesia to survey the genetic diversity of Begomovirus, α and β satellites. A total of 14 samples were tested positive by PCR for tomato leaf curl New Delhi virus (ToLCNDV), pepper yellow leaf curl Indonesia virus (PepYLCIV), mungbean yellow mosaic India virus (MYMIV), and ageratum yellow vein China virus (AYVCNV) infections. Besides that, ageratum yellow vein alphasatellite (AYVA) was identified for the first time in Indonesia, and tomato leaf curl Java betasatellite (ToLCJaB) were detected for the first time in different hosts. Partial genome sequences of begomoviruses and complete sequences of the α and β satellites were recovered and registered in NCBI GenBank (OP846592-OP846615). Phylogenetic study showed that AYVA and ToLCJaB each had two separate lineages. Polymorphism and diversity analyses confirmed other reports that βC1 coding region of ToLCJaB genome is highly conserved. Comparison of phylogroups 1 and 2 of ToLCJaB produced a high FST value, indicated genetic isolation between them. This report could be a basis for future works on the less studied genetic variation of α and β satellites in Indonesia and other countries. Keywords: gene flow, molecular detection, neutrality test, phylogenetic analysis, population study Journal: Plant Protection Science Pages: 317-324 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/16/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/16/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0001.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:16-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ming Jiang Author-Workplace-Name: College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, P.R. China Author-Name: Tong Wang Author-Workplace-Name: College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, P.R. China Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Name: Jesus Simal-Gandara Author-Workplace-Name: Nutrition and Bromatology Group, Department of Analytical Chemistry and Food Science, Faculty of Science, Universidade de Vigo, Ourense, Spain Author-Name: Chandra Nayaka Siddaiah Author-Workplace-Name: Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, India Author-Name: Xiaofeng Dai Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Workplace-Name: Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji, P. R. China Author-Name: Jieyin Chen Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Workplace-Name: Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji, P. R. China Author-Name: Dan Wang Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Author-Name: Zhiqiang Kong Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Title: Overview of the control of plant fungal pathogens by natural products derived from medicinal plants Abstract: Chemical fungicides can cause drug resistance of plant pathogenic fungi, environmental pollution, and potential threats to humans and animals. Therefore, developing low-toxicity, high-efficient and environment-friendly biological control products is critical for green prevention, controlling plant fungal diseases, and maintaining ecological balance. Biocontrol research mainly includes the following aspects: antagonistic microorganisms, fungicidal proteins, RNA interference techniques and botanical fungicides. Significantly, natural products extracted from medicinal plants are valuable repertoire for inhibiting plant fungal diseases. This review systematically reviewed the research advances of using natural products from medicinal plants to inhibit plant pathogenic fungi, including the types of natural products, extraction methods, and antifungal mechanisms. The further prospects for the study and application, which provide the reference for botanical fungicide development and practical application in preventing and controlling plant fungal disease, were also discussed. Keywords: medicinal plants, natural products, fungal disease, biological control, botanical fungicide Journal: Plant Protection Science Pages: 303-316 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/17/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/17/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0002.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:17-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Nanyang Li Author-Workplace-Name: Laboratory of Plant Disease, Hebei University of Engineering, Han Dan, P. R. China Author-Name: Yinping Niu Author-Workplace-Name: Laboratory of Plant Disease, Hebei University of Engineering, Han Dan, P. R. China Author-Name: Xing Wang Author-Workplace-Name: Laboratory of Plant Disease, Hebei University of Engineering, Han Dan, P. R. China Author-Name: Suna Wang Author-Workplace-Name: Laboratory of Plant Disease, Hebei University of Engineering, Han Dan, P. R. China Author-Name: Yuhui Chen Author-Workplace-Name: Laboratory of Eggplant Disease, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, P. R. China Author-Name: Liping Wang Author-Workplace-Name: Laboratory of Plant Disease, Hebei University of Engineering, Han Dan, P. R. China Title: A novel eggplant AP2/ERF transcription factor StPti5 confers resistance to Verticillium wilt across different species Abstract: The APETALA 2/ethylene response factors (AP2/ERFs) are considered essential in plant disease resistance responses. In this study, a novel eggplant AP2/ERF transcription factor gene, StPti5, was identified and functionally described from wild eggplant (Solanum torvum Sw.). The results of bioinformatics analysis show that StPti5 protein contains a DNA-binding AP2/ERF domain and shares high degree of amino acid similarity with the other known AP2/ERF domain. Subcellular localization assay shows that StPti5 protein was localized in the nucleus. Expression of StPti5 was induced by infection with Verticillium dahliae, ethylene, and methyl jasmonate. Analysis of expression patterns suggests that the function of StPti5 in resistance to Verticillium wilt was most closely linked to ethylene signaling. Overexpression of StPti5 in Arabidopsis thaliana could improve disease resistance to V. dahliae and activate genes linked to the ethylene signaling pathway. Studies on the A. thaliana defence response revealed that the formation of reactive oxygen species (ROS) increased considerably following overexpression of StPti5. In summary, our analysis suggest that StPti5 gene is a functional gene that could improve eggplant's resistance to V. dahliae. Keywords: Solanum torvum, ERF transcription factors, Verticillium dahliae, disease resistance, ethylene signaling Journal: Plant Protection Science Pages: 325-336 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/27/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/27/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0003.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:27-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Vendula Hrubešová Author-Workplace-Name: Ivančice-Letkovice, Czech Republic Author-Name: Hana Šefrová Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic Author-Name: Zdeněk Laštůvka Author-Workplace-Name: Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic Title: The importance of local faunal research of moths for plant protection: an example from an agricultural landscape in central Europe Abstract: The study was carried out in the agricultural landscape (mosaic of an urban environment, gardens, grassland, fields, small forests and semi-natural non-forest habitats) in central Europe (southeast Czechia) in 2021. A light trap was used for trapping (207 nights). A total of 485 moth species and 5 170 individuals were captured. Eurytopic species dominated the area, 72% of species and 89% of individuals. Fifty-nine species are considered pests (12%), representing 25% of individuals. The seasonal dynamics of selected harmful species were evaluated and discussed. Species of semi-natural dry and wet habitats were represented by 14%, i.e. a proportion comparable to pests, but with only 4% of individuals. The numbers of species and individuals recorded and the Shannon-Wiener diversity and evenness indices are lower than at natural sites. Yet, overall biodiversity is relatively high, including the presence of some rare, ecologically, or zoogeographically remarkable species. Keywords: agricultural landscape, Lepidoptera, light trap, pests, natural habitat species Journal: Plant Protection Science Pages: 348-355 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/33/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/33/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0004.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:33-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ruchuon Wanna Author-Workplace-Name: Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Workplace-Name: Resource Management in Agricultural Technology Research Unit, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Name: Phirayot Khaengkhan Author-Workplace-Name: Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Workplace-Name: Resource Management in Agricultural Technology Research Unit, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Name: Darika Bunphan Author-Workplace-Name: Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Workplace-Name: Resource Management in Agricultural Technology Research Unit, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Name: Benjapon Kunlanit Author-Workplace-Name: Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Workplace-Name: Resource Management in Agricultural Technology Research Unit, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand Author-Name: Parinda Khaengkhan Author-Workplace-Name: Division of Plant Production Technology, Faculty of Agricultural Technology, Kalasin University, Kalasin, Thailand Author-Name: Hakan Bozdoğan Author-Workplace-Name: Vocational School of Technical Sciences, Department of Plant and Animal Production, Kirşehir Ahi Evran University, Kirşehir, Türkiye Title: Insecticidal activity of essential oil from Jasminum sambac (L.) Aiton against Callosobruchus maculatus (Fabricius, 1775) (Coleoptera: Bruchidae) Abstract: Mungbean seeds [Vigna radiata (L.)] are easily damaged by insects during storage, and essential oils (EOs) have proved effective for controlling insect infestation. This research study assessed the bioactivity of EO from Jasminum sambac (L.) Aiton against Callosobruchus maculatus (F.). The chemical composition of EO was analysed by a gas chromatograph-mass spectrometer (GC-MS). All experiments were conducted following a completely randomised design (CRD) with four replications of the residual contact and seed dressing bioassays. Results indicated that J. sambac EO contained 35 compounds (97.587%), with the main component 3-hexen-1-ol benzoate. Residual contact toxicity LC50 values of this EO on adult C. maculatus at 24, 48, and 72 h were 5.01, 4.00, and 3.13 µL/mL, respectively. J. sambac EO at 8 µL/L showed the highest residual contact efficacy against C. maculatus, killing up to 100% in 72 hours. The highest concentration of J. sambac EO (1 mL/kg) gave optimal protection against C. maculatus within 4 and 5 days with 100% mortality. This concentration showed 100% inhibition of adult F1 progeny emergence, with no seed damage and weight loss of V. radiata L. after 30 days of exposure, and not significantly different from 0.8 mL/kg. Results indicated that J. sambac EO effectively prevented C. maculatus infestation and it could be used as an alternative to commercial chemical insecticides. Keywords: chemical compositions, toxicity, flowers, essential oils, storage insect pests Journal: Plant Protection Science Pages: 369-378 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/69/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/69/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0005.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:69-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Marta Stankiewicz-Kosyl Author-Workplace-Name: Department of Plant Protection, Institute of Horticultural Sciences, Warsaw University of Life Sciences-SGGW, Warsaw, Poland Author-Name: Małgorzata Haliniarz Author-Workplace-Name: Department of Herbology and Plant Cultivation Techniques, University of Life Sciences in Lublin, Lublin, Poland Title: Diversified germination strategies of Centaurea cyanus populations resistant to ALS inhibitors Abstract: Centaurea cyanus is an annual weed mostly infesting winter cereals and rape. The aim of the study was to provide insights into the association between the seed germination characteristics and the herbicide sensitivity in C. cyanus in the presence of the genetic background control. Four populations of this species resistant to acetolactate synthase (ALS) inhibitors were tested. A germination study was conducted in a growth cabinet. Plants were sprayed at the 2- to 3-leaf stage with a field dose of florasulam (5 g/ha). There were four herbicide treatments dates, which included plants that germinated up to the seventh day, between eight and 12, 13 and 15, 16 and 20 days of the germination study. The germination dynamics of the four tested populations of C. cyanus resistant to florasulam was diversified. Three of them reached their maximum germination on the fourth day after sowing, however, the germination of the fourth population was spread over time with the highest number of germinated seeds found seven and twelve days after sowing. The germination time of the plants belonging to the resistant C. cyanus populations differentiated their reaction to florasulam. The conducted study indicated that the germination biology of ALS inhibitor-resistant populations of C. cyanus is diverse, which makes it difficult to introduce universal management strategies of this species into agricultural practice. Integration of control methods is recommended, including delaying the crop sowing date. Keywords: cornflower, florasulam, germination dynamics, herbicide resistance management Journal: Plant Protection Science Pages: 379-388 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/62/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/62/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0006.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:62-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Emese Mergenthaler Author-Workplace-Name: Plant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, Hungary Author-Name: József Fodor Author-Workplace-Name: Plant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, Hungary Author-Name: János Ágoston Author-Workplace-Name: ELKH-SZE PhatoPlant-Lab, Széchenyi István University, Mosonmagyaróvár, Hungary Author-Name: Orsolya Viczián Author-Workplace-Name: Plant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, Hungary Title: Detection and molecular identification of 'Candidatus Phytoplasma asteris' associated with muscari virescence of three grape hyacinth species Abstract: Grape hyacinths are popular perennial, flowering bulbous plants. In 2018, 2019, and 2020, some plants of three Muscari species showed symptoms similar to those associated with phytoplasma infection in commercial ornamental gardens in Hungary. Symptoms included virescence of flowers and yellowing of leaves. Symptomatic and asymptomatic Muscari plants were sampled at two locations to detect and identify the pathogens involved. Total DNA was extracted from the plants and used as a template in polymerase chain reaction assays to amplify 16S rRNA gene sequences and housekeeping genes (tuf, secY) with phytoplasma-specific primers. The resulting PCR products from symptomatic plants were cloned and sequenced bidirectionally. Homology searching of the obtained sequences against the GenBank database indicated the presence of  'Candidatus Phytoplasma asteris' in the three Muscari species. This is the first report worldwide of C. P. asteris phytoplasma infecting M. botryoides and M. comosum, and its first identification in M. armeniacum in Hungary. Keywords: grape hyacinths, phytoplasma, virescence, aster yellows Journal: Plant Protection Science Pages: 389-393 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/70/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/70/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0007.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:70-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Xuemei Xian Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Kunmeng Li Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Hui Liu Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Zhenhui Wang Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Kun Yuan Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Yiyu Hu Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Chengtian Feng Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Meng Wang Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Chunhua Lin Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Qiguang He Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Author-Name: Yu Zhang Author-Workplace-Name: Sanya Nanfan Research Institute of Hainan University, College of Plant Protection, Hainan University, Sanya and Haikou, China Title: Identification and functional analysis of glyoxal oxidase gene from rubber tree anthracnose Abstract: Glyoxal oxidase (GLOX, EC 1.2.3.15) is a class of hydrogen peroxide synthases involved in lignin degradation. Several GLOX genes of biotrophic and necrotrophic phytopathogenic fungi have been confirmed to contribute to fungal growth, development, and pathogenicity. However, the composition, sequence characteristics and functions of genes in the GLOX family of hemi-biotrophic phytopathogenic fungi remain poorly understood. This study identified a GLOX-encoding gene CsGLOX in the hemi-biotrophic phytopathogenic fungus Colletotrichum siamense by bioinformatics analysis and PCR cloning. The encoded protein contains a signal peptide and five cell wall stress-responsive component (WSC) domains at the N terminus, followed by a catalytic structural domain (AA5_1). CsGLOX-deficient mutants were constructed by homologous replacement, which significantly increased the formation of conidia and reduced the pathogenicity and the high-osmotic stress tolerance of the fungus. However, the deletions did not influence fungal filamentous growth. This study proposes that the CsGLOX gene is involved in pathogen conidial formation, pathogenicity, and osmotic stress response, which deepens our understanding of the pathogenic mechanism of hemi-biotrophic phytopathogenic fungus C. siamense. Keywords: glyoxal oxidase, Colletotrichum siamense, pathogenicity, stress responses, pathogenic mechanism Journal: Plant Protection Science Pages: 337-347 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/52/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/52/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0008.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:52-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Mohamed Elimem Author-Name: Rym Jaouadi Author-Workplace-Name: Laboratory of Agriculture Production Systems and Sustainable Development (LR03AGR02), Department of Agricultural Production, Higher School of Agriculture of Mograne, University of Carthage, Mograne-Zaghouan, Tunisia Author-Name: Thameur Bouslema Author-Workplace-Name: LR21AGR03-Production and Protection for a Sustainable Horticulture, University of Sousse, 10 Regional Research Centre on Horticulture and Organic Agriculture, Chott-11 Mariem, Tunisia Author-Name: Maha Kalboussi Author-Workplace-Name: Laboratory of Agriculture Production Systems and Sustainable Development (LR03AGR02), Department of Agricultural Production, Higher School of Agriculture of Mograne, University of Carthage, Mograne-Zaghouan, Tunisia Author-Name: Chaima Lahfef Author-Workplace-Name: Laboratory of Agriculture Production Systems and Sustainable Development (LR03AGR02), Department of Agricultural Production, Higher School of Agriculture of Mograne, University of Carthage, Mograne-Zaghouan, Tunisia Author-Workplace-Name: National Institute of Agronomy of Tunis, University of Carthage, Tunis, Tunisia Author-Name: Slim Rouz Author-Workplace-Name: Laboratory of Agriculture Production Systems and Sustainable Development (LR03AGR02), Department of Agricultural Production, Higher School of Agriculture of Mograne, University of Carthage, Mograne-Zaghouan, Tunisia Author-Name: Hassan Kharroubi Author-Workplace-Name: Higher School of Engineer of Medjez El Beb, University of Jendouba, Medjez ElBeb, Béja, Tunisia Author-Name: Abdennacer Boulila Author-Workplace-Name: Laboratory of Natural Substances, National Institute of Research and Physico-chemical Analyses, Biotechpole of Sidi Thabet, Ariana, Tunisia Author-Name: Sofien Kouki Author-Workplace-Name: Higher School of Engineer of Medjez El Beb, University of Jendouba, Medjez ElBeb, Béja, Tunisia Author-Name: Giuliano Ragnoni Author-Workplace-Name: Basalti Orvieto srl -Loc Cornale, Castek Viscardo, Italy Author-Name: Gianluca Pizzuti Author-Workplace-Name: Basalti Orvieto srl -Loc Cornale, Castek Viscardo, Italy Author-Name: Fabio Primavera Author-Workplace-Name: Basalti Orvieto srl -Loc Cornale, Castek Viscardo, Italy Author-Name: Alessandro Riccini Author-Workplace-Name: Basalti Orvieto srl -Loc Cornale, Castek Viscardo, Italy Title: Assessing the insecticidal effect of Citrus aurantium and Nerium oleander extracts and basalt "Farina di Basalto®" as biological alternatives to control Aphis punicae and Planococcus citri in an organic pomegranate orchard Abstract: This study aims to evaluate the efficiency of basalt with two botanical aqueous extracts of Citrus aurantium and Nerium oleander (50g/L) against Aphis punicae and Planococcus citri in an organic pomegranate orchard. Basalt was applied as a foliar spray with two doses (1.5% and 3%), in the ground as a fertiliser (1.5%), and as a combined application (1.5%). Phenolic compounds of both plants were identified by HPLC-PDA/ESI-MS analysis. Seven and six flavonoids were detected in C. aurantium and N. oleander extracts, respectively. Hesperidin was more abundant in C. aurantium extracts; however, N. oleander extracts contained more quercetin rutinoside. A. punicae populations were significantly higher in control trees compared to the treated ones. A. punicae mortality rates reached high values above 90% for basalt and plants extracts and efficacy rates exceeded 80%. P. citri mortality rates reached 88% and 77%, and efficacy rates exceeded 75% and 66% for basalt and plant extracts, respectively. These findings may help to plan Integrated pest management strategies in organic orchards to avoid toxic chemical pesticides. Keywords: basalt, biological control, extracts, efficacy rate, HPLC, mortality rate Journal: Plant Protection Science Pages: 356-368 Volume: 59 Issue: 4 Year: 2023 DOI: 10.17221/111/2022-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/111/2022-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202304-0009.txt Handle: RePEc:caa:jnlpps:v:59:y:2023:i:4:id:111-2022-PPS