Template-Type: ReDIF-Article 1.0 Author-Name: Vaddi Saitheja Author-Workplace-Name: Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: Kanthan Thirukumaran Author-Workplace-Name: Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: Vaithiyanathan Sendhilvel Author-Workplace-Name: Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: Ramasamy Karthikeyan Author-Workplace-Name: Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: M. Karuppasami Kalarani Author-Workplace-Name: Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: Sampathrajan Vellaikumar Author-Workplace-Name: Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: Panneerselvam Parasuraman Author-Workplace-Name: Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: SP Sangeetha Author-Workplace-Name: Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Author-Name: Thangaraj Abhinaya Author-Workplace-Name: Department of Agronomy, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India Title: Scope and potential of herbicidal values of the fungal pathogens and its secondary metabolites for sustainable weed management Abstract: Weeds are the major menace to agriculture, which greatly impact crop growth and development, resulting in economic yield loss or crop failures. Therefore, it is indispensable to take up appropriate weed management practices to prevent the effects of weeds on crops. Chemical herbicides have immense potential for effective control of weeds, but, in the long run, the persistent nature of herbicides adversely affects the soil microbes and also that terrestrial and aquatic ecosystems. Bioherbicides are products derived from plant extracts, allelochemicals or microbes and their secondary metabolites with weed-suppressing abilities. Most microbial bioherbicides are based on fungi and its active ingredients, which successfully control weeds with different mode of actions. Moreover, the toxins or secondary metabolites the fungi produce also possess herbicidal properties. So, exploring the fungal pathogens and their toxins for managing weeds seems to be a feasible and eco-friendly way for the management of weeds. There is a wider scope for utilizing fungi and their secondary metabolites as mycoherbicides, which have the potential to replace hazardous chemical herbicides in the near future. This review article mainly emphasizes the scope of mycoherbicides and explores the fungal secondary metabolites for eco-friendly weed control. Keywords: biological control, bioformulations, fungi, mycotoxins, weeds Journal: Plant Protection Science Pages: 109-126 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/31/2024-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/31/2024-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0001.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:31-2024-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Hakan Fidan Author-Workplace-Name: Plant Protection Department, Faculty of Agriculture, Akdeniz University, The Campus, Konyaalti, Antalya, Türkiye Author-Name: Ailar Gonbad Author-Workplace-Name: Plant Protection Department, Faculty of Agriculture, Akdeniz University, The Campus, Konyaalti, Antalya, Türkiye Author-Name: Yasin Emre Kitis Author-Workplace-Name: Plant Protection Department, Faculty of Agriculture, Akdeniz University, The Campus, Konyaalti, Antalya, Türkiye Title: Exploring the weed host range of resistance-breaking variants of tomato spotted wilt orthotospovirus (TSWV) across life cycles in Türkiye Abstract: Tomato spotted wilt orthotospovirus (TSWV) is destroying tomato and pepper resistance all over the world, including Antalya (Türkiye). Two greenhouses that show infection of TSWV in the Serik (coastal) and Elmali (highland) areas were chosen for research between 2019 and 2021 to better understand the disease's life cycle. During the surveys, we focused on weed hosts to better understand TSWV disease's cycle. TSWV infection was determined in 58 peppers, 34 tomatoes, 270 weeds, and 20 other vegetable samples. Weed samples revealed essentially no symptoms, however, grown plants showed classic TSWV symptoms. The Asteraceae family had the highest infection rate among infected weeds, followed by weed species from the Poaceae and Solanaceae families. In addition, to determine the viral strain in the infected plant samples, qRT-PCR and Melt-curve analysis were done using a specially designed primer pair for the study. This primer identifies the point mutation on the NSm-movement protein in the viral genome's medium segment. The non-resistance breaking isolate of TSWV was included in the optimisation studies to evaluate differences between the two isolates at two thermal melting values established by this comparison. These findings demonstrated that the kits, procedures, and primers employed in this investigation can serve as a quick and reliable diagnostic tool for identifying TSWV isolates and that weeds are a key intermediate source for new TSWV infection, as confirmed by sequence data. Keywords: resistance breaking isolate, TSWV, diagnostic kit, qRT-PCR, weed Journal: Plant Protection Science Pages: 127-138 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/118/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/118/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0002.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:118-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Markéta Michutová Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic Author-Name: Barbora Mieslerova Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic Author-Name: Ivana Šafránková Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic Author-Name: Barbora Jilkova Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic Author-Name: Maria Neoralova Author-Workplace-Name: Department of Crop Science, Breeding and Plant Medicine, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic Author-Name: Ales Lebeda Author-Workplace-Name: Department of Botany, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic Title: Powdery mildews (Erysiphales) species spectrum on plants of family Lamiaceae in the Czech Republic Abstract: This article focuses on the occurrence of powdery mildews in the Lamiaceae family in the Czech Republic. A comprehensive analysis of morphological characteristics supported by molecular analysis of partial ITS sequences and analysis of host species gained important insights regarding the occurrence and diversity of powdery mildews. Thirty-four plant samples of the Lamiaceae family infected by powdery mildew collected in the Czech Republic between 2015-2022 were analysed. In these samples, the occurrence of all newly described species within the former Golovinomyces biocellatus complex was confirmed (G. biocellatus, G. salviae, G. neosalviae and G. monardae), and Neoërysiphe galeopsidis was also verified. Moreover, our study expands the already reported host species list of G. biocellatus complex within the Lamiaceae family. Keywords: Erysiphaceae, anamorph state, host specificity, internal transcribed spacer region Journal: Plant Protection Science Pages: 139-150 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/21/2024-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/21/2024-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0003.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:21-2024-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Ting Chen Author-Name: Jiling Dang Author-Workplace-Name: Shuifa Haohai (Jiuquan) Agricultural Development Co., Ltd, Jiuquan, Gansu, P. R. China Author-Name: Peng Zhang Author-Workplace-Name: Shuifa Haohai (Jiuquan) Agricultural Development Co., Ltd, Jiuquan, Gansu, P. R. China Author-Name: Jinju Shi Author-Workplace-Name: Shuifa Haohai (Jiuquan) Agricultural Development Co., Ltd, Jiuquan, Gansu, P. R. China Author-Name: Jia Feng Author-Workplace-Name: School of Life Science, Shanxi University, Taiyuan, Shanxi, P. R. China Title: Preliminary study on horizontal transfer and loss of the AM toxin gene of Alternaria Abstract: The genus Alternaria has a global distribution and consists of a diverse group of pathogens. Plant-pathogenic Alternaria spp. can reduce the crop yield and pose serious threats to agricultural production. The pathogen A. mali is recognised as the key the pathogenic mechanism in the early defoliation of apples, which produces the host specific toxin (HST) that was named as an apple specific toxin (a specialised toxin of A. alternata pv. mali, AM toxin). The phenomenon of horizontal transfer of the AM toxin gene from different strains of A. alternata was found, and the relationship between the AM toxin and pathogenicity was confirmed. The representative strain A. tuberculata with the AM toxin gene was co-cultured with sixteen Alternaria strains without the AM toxin gene. As a result, four strains from different Alternaria species obtained the AM toxin gene, which indicated that the AM toxin gene can transfer among different Alternaria species. The AM toxin gene is easy to be lost after subculture, and high temperature and low nutrition can promote this loss. The symptoms of the Alternaria pathogen with or without the AM toxin gene are obviously different on the host. When infected by a pathogen with the AM toxin gene, green spots formed on the apple leaves, and rotten disease spots appeared in the fruit carpels. Contrary to this, when infected by a pathogen without this gene, only small epidermal spots without chlorosis formed on the apple leaves, and mildew-heart spots appeared in the fruit carpels. Keywords: Alternaria spp., AM toxin, horizontal transfer, pathogenicity Journal: Plant Protection Science Pages: 151-160 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/106/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/106/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0004.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:106-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: He Zhu Author-Workplace-Name: National Cotton Industry Technology System Liaohe Comprehensive Experimental Station, The Cotton Research Center of Liaoning Academy of Agricultural Sciences, Liaoning Provincial Institute of Economic Crops, Liaoyang, P. R. China Author-Name: Yue Li Author-Workplace-Name: National Cotton Industry Technology System Liaohe Comprehensive Experimental Station, The Cotton Research Center of Liaoning Academy of Agricultural Sciences, Liaoning Provincial Institute of Economic Crops, Liaoyang, P. R. China Author-Name: Xiao-Bin Ji Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China Author-Name: Dan-Dan Zhang Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China Author-Workplace-Name: Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji, P. R. China Author-Name: Jie-Yin Chen Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China Author-Workplace-Name: Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji, P. R. China Author-Name: Xiao-Feng Dai Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China Author-Workplace-Name: Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji, P. R. China Author-Name: Zi-Sheng Wang Author-Workplace-Name: National Cotton Industry Technology System Liaohe Comprehensive Experimental Station, The Cotton Research Center of Liaoning Academy of Agricultural Sciences, Liaoning Provincial Institute of Economic Crops, Liaoyang, P. R. China Author-Name: Dan Wang Author-Workplace-Name: The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China Author-Workplace-Name: State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou, P. R. China Title: The disease quantification analysis of cotton Verticillium wilt using the two methods of disease index and fungal biomass present high consistency Abstract: Verticillium dahliae is a broad host-range pathogen that causes vascular wilt in plants. The Verticillium wilt disease severity assay on plants caused by V. dahliae mainly includes two methods, one is a plant disease grade classification based on disease severity statistics [namely the disease index (DI)], and the other is the V. dahliae biomass quantification in plants (namely the fungal biomass). In this study, the relationships of pathogenicity with the DI, pathogenicity with the biomass, and the correlation analysis of the DI and relative fungal biomass were analysed. The results showed that pathogenicity assessment of V. dahliae strains using the DI method was able to give an intuitive reflection of the pathogenic ability for defoliating and non-defoliating strains; moreover, the method of quantitative PCR for fungal biomass also had high repeatability and stability. As a whole, the correlation coefficient between the DI and fungal biomass values of 28 strains was 0.728, indicating that the two data sets were highly correlated; however, the correlation coefficients of the defoliating strains and non-defoliating strains were only 0.5384 and 0.4547, respectively. In conclusion, the correlation coefficient between the DI and the fungal biomass presented high consistency, which could provide some meaningful exploration for the more accurate pathogenicity identification of V. dahliae. Keywords: Verticillium dahliae, pathogenicity, disease grade classification, qPCR, correlation coefficient Journal: Plant Protection Science Pages: 161-171 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/109/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/109/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0005.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:109-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Lei Yang Author-Workplace-Name: Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei, P. R. China Author-Name: Xue Ma Author-Workplace-Name: Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei, P. R. China Author-Name: Lijun Wang Author-Workplace-Name: College of Life Sciences, Yantai University, Yantai, P. R. China Author-Name: Guogen Yang Author-Workplace-Name: Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei, P. R. China Author-Name: Lijun Zhou Author-Workplace-Name: Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei, P. R. China Author-Name: Zhilin Zhang Author-Workplace-Name: Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, College of Life Science and Technology, Hubei Engineering University, Xiaogan, P. R. China Author-Name: Xiaomeng Li Author-Workplace-Name: Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei, P. R. China Title: In vitro antifungal activity and mechanism of action of carvacrol against Sclerotinia sclerotiorum (Lib.) de Bary Abstract: This study aimed to examine the antifungal effects of nine monoterpenes on Sclerotinia sclerotiorum via the mycelial growth rate method. The effects of carvacrol on the morphological structures of hyphae were investigated by scanning electron microscopy (SEM). The oxalic acid (OA), total protein contents, and the activity of cell wall-degrading enzymes, including chitinase, cellulase and β-1,3-glucanase, were assessed. The results showed that the antifungal rates of carvacrol, thymol and eugenol reached 100% at a concentration of 400 mg/L, and the EC50 values of carvacrol, thymol, and eugenol were 43.40, 56.22, and 86.63 mg/L, respectively. The treatment of S. sclerotiorum with carvacrol had no significant effect on sclerotia formation, but the mycelial surface was shrivelled, uneven and broken, with cytoplasm flowing out. The OA content of S. sclerotiorum was significantly reduced to 133.78 µg/mL after treatment with carvacrol. Additionally, the total protein content of S. sclerotiorum mycelia in the carvacrol treatment group was significantly reduced to 15.67 µg/mL compared with that of the control group, and the activity of cellulase in the carvacrol treatment group was significantly higher than that in the control group. Keywords: monoterpenes, morphological structures of hyphae, oxalic acid, total protein content, cellulase Journal: Plant Protection Science Pages: 172-180 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/121/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/121/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0006.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:121-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: P.S. Shanmugam Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: T. Srinivasan Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: V. Baskaran Author-Workplace-Name: Agricultural College & Research Institute, TNAU, Tiruvannamalai, Tamil Nadu, India Author-Name: A. Suganthi Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: B. Vinothkumar Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: G. Arulkumar Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: S. Backiyaraj Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: S. Chinnadurai Author-Workplace-Name: Agricultural College & Research Institute, TNAU, Tiruvannamalai, Tamil Nadu, India Author-Name: V. Somasundaram Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: N. Sathiah Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: N. Muthukrishnan Author-Workplace-Name: Agricultural College & Research Institute, TNAU, Tiruvannamalai, Tamil Nadu, India Author-Name: S.V. Krishnamoorthy Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: K. Prabakar Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: S. Douresamy Author-Workplace-Name: Agricultural College & Research Institute, TNAU, Tiruvannamalai, Tamil Nadu, India Author-Name: Y.S. Johnson Edward Thangaraj Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: S. Pazhanivelan Author-Workplace-Name: Water Technology Centre, TNAU, Coimbatore, Tamil Nadu, India Author-Name: K.P. Ragunath Author-Workplace-Name: Water Technology Centre, TNAU, Coimbatore, Tamil Nadu, India Author-Name: R. Kumaraperumal Author-Workplace-Name: Department of Remote Sensing & GIS, TNAU, Tamil Nadu, India Author-Name: S. Jeyarani Author-Workplace-Name: Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore, Tamil Nadu, India Author-Name: R. Kavitha Author-Workplace-Name: Department of Farm Machinery and Power Engineering, TNAU, Coimbatore, Tamil Nadu, India Author-Name: A.P. Mohankumar Author-Workplace-Name: Department of Farm Machinery and Power Engineering, TNAU, Coimbatore, Tamil Nadu, India Title: Comparative analysis of unmanned aerial vehicle and conventional spray systems for the maize fall armyworm Spodoptera frugiperda (J.E. Smith) (Lepidoptera; Noctuidae) management Abstract: Insecticidal interventions at critical stages of maize are an important strategy for managing invasive insect pest fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith). Conventional spraying systems cannot be used over larger areas, and the insecticide application using unmanned aerial vehicles is becoming popular among peasants. As the FAW resides inside the maize whorls, targeted insecticide application is necessary for effective management. The efficacy of (UAV) spray with different types of nozzles was compared with the conventional spray system, namely high-volume spray and Control droplet applicator. The other spray systems' droplet density, efficacy, and residues of insecticides in plants, soil and water were studied. The UAV droplet density up to 5 m swath recorded no significant variation for both nozzles. A UAV with an atomizer nozzle was as effective as a high-volume spray in reducing the FAW infestation. The residue analysis of leaf samples from the study area revealed more residues in the control droplet applicator and UAV atomizer nozzle. The per cent reduction of initial deposits in the top, middle and bottom maize leaves was least in the UAV atomizer nozzle. The insecticide residues in the study sample area were also below the detectable limit. UAV usage in maize saves time and reduces FAW damage as that of high-volume sprayers. Keywords: atomizer nozzle, flat fan nozzle, high volume spray, control droplet applicator Journal: Plant Protection Science Pages: 181-192 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/96/2023-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/96/2023-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0007.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:96-2023-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Renata Dobosz Author-Workplace-Name: Institute of Plant Protection-National Research Institute, Poznan, Poland Author-Name: Roman Krawczyk Author-Workplace-Name: Institute of Plant Protection-National Research Institute, Poznan, Poland Title: Effect of legume (Fabaceae Lindl.) seeds on selected life activities in J2 stage of Meloidogyne hapla Abstract: Meloidogyne hapla is a serious pest of many cultivated plants. In response to the economic significance of the species, efforts are being made to develop a new method to reduce its harmful effects on crops. The aim of the study was to determine the effect of diffusates from seeds of selected species of legumes on the motility of second-stage juveniles and to evaluate the effect of meal from seeds of selected species of legume on the capacity to infect the roots of tomato plants by the J2 stage. The experiment examined the effect of diffusates on the motility of the J2 stage performed in Petri dishes, at temperatures of 10 °C, 17 °C and 21 °C. The evaluation of the J2 stage infectivity was estimated in a pot experiment performed under controlled conditions of 20 ± 1 °C. The pots were filled with sterile substrate mixed with meal from the seeds of selected plants at 1%, 5% and 10% of the substrate weight. The studies carried out in the Petri dishes showed varying effects of the seed diffusates from selected legume plants on the motility of the J2 stage of Meloidogyne hapla. J2 were found to lose their motility within 24 h after immersion in water containing seed diffusates from Melilotus albus, Trifolium pratense T. repens, in the temperature ranges investigated (10 °C, 17 °C and 21 °C). However, in a mixture of seed diffusates and soil filtrate from the root zone of tomato plants, the absence of motility in the second-stage juveniles was observed after 24 h at 17 °C and 21 °C, with seed diffusates from Lotus corniculatus, Medicago sativa, Medicago × varia, Melilotus officinalis, as well as Onobrychis viciifolia, Ornithopus sativus, Vicia sativa, used in the mixture. Galega officinalis Risa (GoR) seed diffusates were found to have an inhibiting effect on the motility of the J2 stage of M. hapla 24 h following the immersion of the J2 stage in the solution of the soil filtrate containing tomato root diffusates, at 21 °C. The J2 stage were not rendered immotile in all the experiment set-ups involving the seeds of V. faba, Lupinus spp., likewise in the control set-ups. In the pots studied, a significant effect of the addition of legume seed meal on the development of M. hapla nematodes and tomato plants was found. The introduction of Lotus corniculatus, Onobrychis viciifolia and Vicia sativa seed meal into the substrate in the proportion of 1%, 5% and 10% resulted in the inhibition of the J2 stage penetration into the roots of tomato plants at temperatures of 17 °C and 21 °C. With the admixture of the M. sativa and T. repens seed meal, within the temperature range investigated, no nematode infection was observed in the roots, regardless of the seed meal content in the substrate. As regards to the fresh weight, tomato plants grown in a substrate containing 1% and 5% of the V. sativa cv. Jaga seed meal were characterised by significantly higher plant weight values as compared to those grown in the control set-up. The obtained results imply that is advisable to expand the scope of research to include other economically important crops damaged by the northern root-knot nematode. Keywords: Meloidogyne hapla, motility, infectivity, legumes, seed diffusates, seed meal Journal: Plant Protection Science Pages: 193-206 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/17/2024-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/17/2024-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0008.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:17-2024-PPS Template-Type: ReDIF-Article 1.0 Author-Name: Peter Tóth Author-Workplace-Name: International Network of Eco-Regions (IN.N.E.R.), Salerno, Italy Author-Name: Monika Tóthová Author-Workplace-Name: Institute of Agronomic Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Slovak Republic Author-Name: Nikola Andjelković Author-Workplace-Name: Department of Entomology and Agricultural Zoology, Faculty of Agriculture, Belgrade-Zemun, University of Belgrade, Serbia Author-Name: Slavica Marinković Author-Workplace-Name: Institute for Plant Protection and Environment, Belgrade, Serbia Author-Name: Tatjana Cvrković Author-Workplace-Name: Institute for Plant Protection and Environment, Belgrade, Serbia Author-Name: Biljana Vidović Author-Workplace-Name: Department of Entomology and Agricultural Zoology, Faculty of Agriculture, Belgrade-Zemun, University of Belgrade, Serbia Title: Aceria artemisiifoliae Vidović & Petanović (Acari: Eriophyoidea) on common ragweed - the second record in the world Abstract: Common ragweed - Ambrosia artemisiifolia L. (Asteraceae) is an invasive plant species in Europe native to North America. Most of the records of known eriophyid mites on different ragweed species are from their native range. Our field experiments in Slovakia, 2016-2023, aimed to identify specific species feeding on common ragweed. We searched for symptomatic plants and collected growing tips, which were then preserved in 70% ethanol for further study. A recently described species of eriophyid mite, Aceria artemisiifoliae Vidović & Petanović (Acari: Eriophyoidea), was found in western and eastern Slovakia. This is the first record of the species in Slovakia and the second record in the world. It remains unclear whether this species is invasive like Ambrosia, and whether it could be used as a potential biological control agent. Keywords: eriophyid mite, new record, invasive species, Ambrosia artemisiifolia, leafy rosette Journal: Plant Protection Science Pages: 207-211 Volume: 60 Issue: 2 Year: 2024 DOI: 10.17221/13/2024-PPS File-URL: http://pps.agriculturejournals.cz/doi/10.17221/13/2024-PPS.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pps-202402-0009.txt Handle: RePEc:caa:jnlpps:v:60:y:2024:i:2:id:13-2024-PPS