Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: Index of Volume 50 Journal: Plant, Soil and Environment Pages: I-VI Volume: 50 Issue: 12 Year: 2004 DOI: 10.17221/4073-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/4073-PSE.html File-Format: text/html Handle: RePEc:caa:jnlpse:v:50:y:2004:i:12:id:4073-PSE Template-Type: ReDIF-Article 1.0 Author-Name: D. Pavlíková Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: T. Macek Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: M. Macková Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: M. Surá Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Száková Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: P. Tlustoš Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Title: The evaluation of cadmium, zinc and nickel accumulation ability of transgenic tobacco bearing different transgenes Abstract: Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control (WSC), and four genetically modified lines of the same variety, were tested for Cd, Zn and Ni accumulation. Genetically modified lines of the same variety, bearing the transgene CUP1 (gene coding a yeast metallothionein), GUS (reporter gene for ß-glucuronidase), HisCUP (CUP combined with a polyhistidine tail), and HisGUS (reporter gene for ß-glucuronidase, combined with a polyhistidine tail) under a constitutive promoter, enabling it to follow the heavy metal tolerance and uptake changes as a function of the transgene present. Control and transgenic lines were tested for accumulation of risk elements on sand nutrient medium with the addition of cadmium, zinc and nickel. The results showed high Cd accumulation ability of HisCUP line. The Cd content in aboveground biomass was increased by 90% compared to the non-transformed control and Cd content in roots was decreased by 49%. Determination of Zn content in aboveground biomass did not confirm higher uptake by transgenic plants significant for phytoremediation. The Ni content was significantly increased in aboveground biomass of HisGUS construct. GUS construct introduced the ability to accumulate all investigated metals; the others accumulated only one in extended amount. Keywords: transgenic tobacco, metallothionein, polyhistidine, ß-glucuronidase, cadmium, zinc, nickel, accumulation Journal: Plant, Soil and Environment Pages: 513-517 Volume: 50 Issue: 12 Year: 2004 DOI: 10.17221/4067-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/4067-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-200412-0002.txt Handle: RePEc:caa:jnlpse:v:50:y:2004:i:12:id:4067-PSE Template-Type: ReDIF-Article 1.0 Author-Name: H.W. Scherer Author-Workplace-Name: Agricultural Faculty of the University of Bonn, Germany Title: Influence of compost application on growth and phosphorus exploitation of ryegrass (Lolium perenne L.) Abstract: Information on the availability of P following compost addition to soil may help to a better management of P fertilization of the crop in respect to plant growth and the environment. Therefore a greenhouse experiment was conducted to investigate the effect of compost within the official classes of stability III and V on growth and P exploitation of ryegrass (Lolium perenne L. cv. Turilo). The following result was obtained: As compared to a control without P application, compost application resulted in a significant yield increase. Application of composts of the official class of stability V resulted in higher yields as compared with the application of compost of the official class of stability III. P uptake of ryegrass was higher in the treatments in the class of stability V. Based on CAL-extractable compost P exploitation of P ranged between 8.5 and 104.0% in the first year of compost application and in total (sum of two years) 37.8 and 204.5%. P exploitation on the basis of total compost P ranged between 3.6 and 22.1%. Keywords: compost application, phosphorus, availability, exploitation, ryegrass, yield formation Journal: Plant, Soil and Environment Pages: 518-524 Volume: 50 Issue: 12 Year: 2004 DOI: 10.17221/4068-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/4068-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-200412-0003.txt Handle: RePEc:caa:jnlpse:v:50:y:2004:i:12:id:4068-PSE Template-Type: ReDIF-Article 1.0 Author-Name: K. Šichorová Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: P. Tlustoš Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Száková Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: K. Kořínek Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Balík Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Title: Horizontal and vertical variability of heavy metals in the soil of a polluted area Abstract: The Příbram region belongs to the most polluted areas in the Czech Republic. Atmospheric deposition of potentially toxic elements from lead smelter represents the main source of contamination in this region. In this study, the most polluted location intensively used for agriculture situated northeast from the source of contamination was investigated. The individual sampling points were positioned by GPS and representative samples were taken from 0-60 cm split into three20 cmlayers. In the soil samples total Pb and Cd contents, plant available contents of these toxic elements and soil pH were determined. The total Cd content at the investigated site reached up to 13 mg/kg and Pb up to 2500 mg/kgin the top layer. The concentration of both elements decreases with depth and in the 40-60 cm layer the maximum content of only 6 mg Cd/kg and 400 mg Pb/kg was found. The plant available the content of Pb and Cd in the soil is related to the pH value. The correlation coefficient of exponential regression for Cd is -0.799 and for Pb is -0.787. The obtained data was processed by Surfer 7 software resulting in digital maps of horizontal and vertical contamination of this location. This observation confirmed the airborne source of soil contamination. However, some of the individual sampling points suggested local geogenic contamination of the soil where more detailed further research is required. Keywords: potentially toxic elements, cadmium, lead, spatial variability, soil, polluted area Příbram Journal: Plant, Soil and Environment Pages: 525-534 Volume: 50 Issue: 12 Year: 2004 DOI: 10.17221/4069-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/4069-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-200412-0004.txt Handle: RePEc:caa:jnlpse:v:50:y:2004:i:12:id:4069-PSE Template-Type: ReDIF-Article 1.0 Author-Name: V. Kovačević Author-Workplace-Name: Faculty of Agriculture, University of J.J. Strossmayer in Osijek, Croatia Author-Name: I. Brkić Author-Workplace-Name: Faculty of Agriculture, University of J.J. Strossmayer in Osijek, Croatia Author-Name: D. Šimić Author-Workplace-Name: Faculty of Agriculture, University of J.J. Strossmayer in Osijek, Croatia Author-Name: G. Bukvić Author-Workplace-Name: Faculty of Agriculture, University of J.J. Strossmayer in Osijek, Croatia Author-Name: M. Rastija Author-Workplace-Name: Faculty of Agriculture, University of J.J. Strossmayer in Osijek, Croatia Title: The role of genotypes on phosphorus, zinc, manganese and iron status and their relations in leaves of maize on hydromorphic soil Abstract: Hydromorphy is a major soil fertility constraint in Europe and North America causing specific nutritional imbalance in soils and crops. The objectives of this study were to examine the differences among maize hybrids in phosphorus (P), zinc (Zn), manganese (Mn) and iron (Fe) status in leaves, and to investigate the interrelationship among these micronutrients as well as the interrelationship between Zn and P on hydromorphic soil in Eastern Croatia. Twenty maize (Zea mays L.) hybrids were grown under field conditions of Eastern Croatia in a period of two years. Considerable differences in mean ear-leaf Zn, Mn and Fe contents at silking stage were found ranging from 16.3 to 30.0 mg/kg for Zn, from 28.5 to 62.2 mg/kg for Mn and from 137 to 222 mg/kg for Fe content. Mean ratios among nutrients were at acceptable levels: 146 (P:Zn), 7.6 (Fe:Zn) and 3.72 (Fe:Mn). Differences among the hybrids ranged from 95.1 to 210.4 (P:Zn), from 5.3 to 10.4 (Fe:Zn) and from 2.4 to 5.79 (Fe:Mn). Maize hybrids which have higher P:Zn is less acceptable for growing on soils which are either rich in P or moderate in available Zn. Highly significant correlation coefficients between years for mineral content (0.696 for Zn, 0.586 for Mn and 0.525 for Fe) indicated high repeatability estimates within genotypes across the seasons and importance of heredity for nutritional status. Keywords: maize hybrids, phosphorus, zinc, manganese, iron, hydromorphic soil Journal: Plant, Soil and Environment Pages: 535-539 Volume: 50 Issue: 12 Year: 2004 DOI: 10.17221/4070-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/4070-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-200412-0005.txt Handle: RePEc:caa:jnlpse:v:50:y:2004:i:12:id:4070-PSE Template-Type: ReDIF-Article 1.0 Author-Name: P. Tlustoš Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: W. Gössler Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Száková Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: D. Pavlíková Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Balík Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Title: Arsenic compounds in the leaves and roots of radish grown in three soils treated by dimethylarsinic acid Abstract: The effect of dimethylarsinic acid (DMA) on the growth of radish and the content of As compounds in roots and leaves of the radish were investigated. Radish was grown in pots in three soils (Fluvisols, Chernozems and Luvisols) amended with 20 mg As/kg of soil in form of DMA. Behavior and transformations of DMA in the soils differed depending on the individual soil type. In the first season, plants grew up at Luvisols only. In the second season the plants were able to grow at Luvisols and Chernozems, too. The roots and leaves of radish from Luvisols had DMA as the dominant arsenic compound present (~ 90% in the extract) in the first season. In the roots of the subsequently growing radish DMA accounted for 20% and arsenite for most of the total arsenic in the extract. In the leaves of the second-crop of radish DMA remained the dominant arsenic compound (~ 60% in the extract) with arsenate and arsenite for the remaining 40%. Roots and leaves of radish grown in Chernozems and Luvisols had a similar pattern of arsenic compounds. Soil properties significantly affected transformation of As species in the soils. At Fluvisols was the lowest As immobilization and about 98% was found as DMA after two years of experiments in the other two soils was higher As immobilization and DMA was recovered to inorganic As (V) - 31% in Luvisols and 78% in Chernozems. Keywords: arsenate, arsenite, dimethylarsinic acid, HPLC-ICP-MS, plant-availability, radish, soil properties, extraction Journal: Plant, Soil and Environment Pages: 540-546 Volume: 50 Issue: 12 Year: 2004 DOI: 10.17221/4071-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/4071-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-200412-0006.txt Handle: RePEc:caa:jnlpse:v:50:y:2004:i:12:id:4071-PSE Template-Type: ReDIF-Article 1.0 Author-Name: K. Štípek Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: V. Vaněk Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Száková Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Černý Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Author-Name: J. Šilha Author-Workplace-Name: Czech University of Agriculture in Prague, Czech Republic Title: Temporal variability of available phosphorus, potassium and magnesium in arable soil Abstract: The investigation is focused on the illustration of the temporal changes in spatial variability of the Mehlich III available phosphorus, potassium and magnesium in the 10.5 hapart of the whole 54 hafield, located near Český Brod. Soil is characterized as Orthic Luvisol. To estimate temporal variability of available nutrients, soil samples were taken in 2001-2003 four times per year (twice in spring and twice in the autumn periods). For the description of field variability of selected soil parameters coefficient of variation (CV), experimental variograms with fitted models and relative nugget effect parameters have been used. Three year results shows that the lowest temporal variability, characterized by relative nugget effect had available Mg (4-23%) and P (13-29%) and K (15-49%). Keywords: temporal variability, spatial variability, phosphorus, potassium, magnesium, Mehlich III Journal: Plant, Soil and Environment Pages: 547-551 Volume: 50 Issue: 12 Year: 2004 DOI: 10.17221/4072-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/4072-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-200412-0007.txt Handle: RePEc:caa:jnlpse:v:50:y:2004:i:12:id:4072-PSE