Template-Type: ReDIF-Article 1.0 Author-Name: R. Duffková Author-Workplace-Name: Research Institute for Soil and Water Conservation, Prague, Czech Republic Author-Name: H. Libichová Author-Workplace-Name: Research Institute for Soil and Water Conservation, Prague, Czech Republic Title: Effects of cattle slurry application on plant species composition of moderately moist Arrhenatherion grassland Abstract: Cattle slurry is frequently used fertilizer on grasslands, but little is known about its effect on plant species composition. The aim of this study was therefore to assess effect of different application rates of cattle slurry (S0 - 0, S1 - 60, S2 - 120, S3 - 180, S4 - 240 kg N/ha/year) on the plant species composition of three-cut grassland. The study was performed over 6 years on moderately moist upland Arrhenatherion grassland in the Czech Republic dominated by Alopecurus pratensis, Trisetum flavescens, and Poa spp. Species composition recorded in treatments with application of cattle slurry in rate up to 120 kg N/ha/year was similar to the unfertilized control. During first three years, species richness was similar in all treatments and then decreased the most in S4 followed by S3 treatment. Cover of short forbs increased in S0 and decreased with an increase in slurry application rate which supported tall grasses. Application of cattle slurry up to 120 kg N/ha/year can be considered as suitable compromise between maintenance of species rich grasslands and requirements of farmers for sufficient forage production. Keywords: species richness, water and nutrient availability, functional groups Journal: Plant, Soil and Environment Pages: 485-491 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/62/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/62/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0001.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:62-2013-PSE Template-Type: ReDIF-Article 1.0 Author-Name: Y. Xu Author-Workplace-Name: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China Author-Name: W. Yu Author-Workplace-Name: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China Author-Name: Q. Ma Author-Workplace-Name: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China Author-Name: H. Zhou Author-Workplace-Name: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China Title: Accumulation of copper and zinc in soil and plant within ten-year application of different pig manure rates Abstract: Fertilization of crops with pig manure is a common practice throughout the world. Nevertheless, due to the relatively high copper (Cu) and zinc (Zn) contents in pig manure, continuous application of pig manure could have negative effects on soil and plant. The study aimed at the impacts of long-term applying different pig manure rates (equivalently 0, 100, 250 and 500 kg total N/ha/year from 2002 to 2008 and 0, 10, 25 and 50 t fresh weight/ha/year from 2009 to 2011, respectively) on Cu and Zn accumulation in soil and plant. During the 10 years of the experiment, a total of 2.04 to 10.20 kg/ha/year for Cu, 3.15 to 15.73 kg/ha/year for Zn were applied to the soil. Results from this study showed that long-term pig manure application resulted in serious accumulation of Cu and Zn in soil, total Cu and Zn concentrations increased by 204% and 107% at high application rates, respectively. Although topsoil Cu and Zn concentrations were below concentrations considered phytotoxic to crops, according to current Chinese legislation, it would take only less time than 16 and 27 years of high application rates to reach the allowable limits. Our result also suggested that Cu and Zn leaching occurred in the tested soil. The Cu and Zn concentrations in stalks and grains were not affected by the application of pig manure, and these values were lower than the threshold values for animal and human ingestion. Keywords: long-term field experiments, maize, soybean, DTPA, leaching Journal: Plant, Soil and Environment Pages: 492-499 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/121/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/121/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0002.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:121-2013-PSE Template-Type: ReDIF-Article 1.0 Author-Name: J. Wyszkowska Author-Workplace-Name: Department of Microbiology, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Borowik Author-Workplace-Name: Department of Microbiology, University of Warmia and Mazury, Olsztyn, Poland Author-Name: J. Kucharski Author-Workplace-Name: Department of Microbiology, University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Baćmaga Author-Workplace-Name: Department of Microbiology, University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Tomkiel Author-Workplace-Name: Department of Microbiology, University of Warmia and Mazury, Olsztyn, Poland Author-Name: E. Boros-Lajszner Author-Workplace-Name: Department of Microbiology, University of Warmia and Mazury, Olsztyn, Poland Title: The effect of organic fertilizers on the biochemical properties of soil contaminated with zinc Abstract: This study evaluates the effectiveness of organic fertilizers in restoring the homeostasis of soils contaminated with zinc. The activity of selected enzymes participating in the transformation of carbon, nitrogen, phosphorus and sulfur and the sensitivity of white mustard plants to zinc were analyzed. A greenhouse pot experiment was carried out. Uncontaminated soil served as control. Six organic substances which potentially neutralize the adverse effects of zinc were used: tree bark, finely ground barley straw, pine sawdust, cattle manure, compost and cellulose. It was found that in less contaminated soil (300 mg Zn2+/kg), all of the analyzed organic substances minimized zinc adverse effects on the biochemical properties of soil, including the activity of dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, β-glucosidase and arylsulfatase. In more contaminated soil (600 mg Zn2+/kg), the negative consequences of zinc pollution were effectively mitigated only by cellulose, barley straw and manure. Cellulose had the highest soil restoration potential, as demonstrated by resistance indicator values for different enzymes. Cellulose, compost, manure and straw increased the resistance of white mustard plants to zinc, but only in treatments contaminated with 300 mg Zn2+/kg. Bark and sawdust potentiated zinc toxic effects on mustard plants. Keywords: soil pollution, enzyme activity, resistance index, organic fertilization Journal: Plant, Soil and Environment Pages: 500-504 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/537/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/537/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0003.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:537-2013-PSE Template-Type: ReDIF-Article 1.0 Author-Name: M. Abid Author-Workplace-Name: Department of Soil Science, Bahauddin Zakariya University, Multan, Pakistan Author-Name: N. Ahmed Author-Workplace-Name: Department of Soil Science, Bahauddin Zakariya University, Multan, Pakistan Author-Name: M.F. Qayyum Author-Workplace-Name: Department of Soil Science, Bahauddin Zakariya University, Multan, Pakistan Author-Name: M. Shaaban Author-Workplace-Name: College of Resources and Environment, Huazhong Agricultural University, Wuhan, P.R. China Author-Name: A. Rashid Author-Workplace-Name: Biosciences Division, Pakistan Atomic Energy Commission, Islamabad, Pakistan Title: Residual and cumulative effect of fertilizer zinc applied in wheat-cotton production system in an irrigated aridisol Abstract: The objectives of present study were to determine the residual and cumulative effects of zinc (Zn) fertilizer on cotton (Gossypium hirsutum L.) and wheat (Triticum aestivum L.) in a silt loam Typic Haplocambid soil (< 0.05 mg/kg diethylenetriaminepentaacetic acid (DTPA)-Zn). The study comprised of two years field experiments where first cotton crop received zinc sulphate (ZnSO4∙H2O) at five rates (0, 5, 7.5, 10, 12.5 kg Zn/ha) in a randomized complete block design with four replications. After harvest, each plot was divided into two sub-plots. To study the residual effect, one sub-plot of all plots did not receive Zn fertilizer for the subsequent crops; however, the other sub-plot received all Zn rates for 2005-06 wheat, 2006 cotton, and 2006-07 wheat. Fresh applied, residual as well as cumulative Zn application significantly (P ≤ 0.05) increased crops production for both experimental years. Residual effect of 5.0 kg Zn/ha optimized the 2006 cotton yield; however, wheat productivity was optimized with residual effect of 7.5 kg Zn/ha in 2005-06 and of 10.0 kg Zn/ha in 2006-07. Optimum yield of both crops was attained with a lesser fresh-applied and residual Zn rate than cumulative Zn rate. Total Zn uptake by wheat (134.9-289.6 g/ha) was much greater than by cotton (92.3-192.5 g/ha). It is concluded that one application of 7.5 kg Zn/ha proved adequate for optimizing two cycles of the cotton-wheat production system. Two-year repeated use of 5.0-7.5 kg Zn/ha did not depress crop yields. Keywords: calcareous soil, crops production, micronutrient Journal: Plant, Soil and Environment Pages: 505-510 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/313/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/313/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0004.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:313-2013-PSE Template-Type: ReDIF-Article 1.0 Author-Name: J.L. Hu Author-Workplace-Name: State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hong Kong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China Author-Name: A.N. Zhu Author-Workplace-Name: State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hong Kong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China Author-Name: J.H. Wang Author-Workplace-Name: State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hong Kong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China Author-Name: J. Dai Author-Workplace-Name: State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hong Kong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China Author-Name: J.T. Wang Author-Workplace-Name: State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hong Kong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China Author-Name: R.R. Chen Author-Workplace-Name: State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hong Kong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China Author-Name: X.G. Lin Author-Workplace-Name: State Key Laboratory of Soil and Sustainable Agriculture, Joint Open Laboratory of Soil and the Environment, Hong Kong Baptist University and Institute of Soil Science, Chinese Academy of Sciences, Nanjing, P.R. China Title: Soil microbial metabolism and invertase activity under crop rotation and no-tillage in North China Abstract: Soil samples were collected at both jointing and maturing stages of maize and wheat to compare the effects of 4-year no-tillage (NT) and conventional tillage (CT) on seasonal variations of microbial biomass carbon (C), metabolic quotient, and invertase activity in a sandy loam soil in North China. Soil invertase activity significantly increased (P < 0.05) from summer to spring of the next year and then significantly decreased (P < 0.05) from spring to summer. With a delay of about 3 months, soil microbial biomass C and basal respiration altered in a similar pattern, while microbial metabolic quotient changed on the contrary. Compared with CT, the NT practice significantly increased (P < 0.05) soil organic C content, and tended to result in higher soil microbial biomass C and invertase activity, as well as lower soil microbial metabolic quotient, especially at the jointing stage of maize. Our results indicated that NT might play an important role in the improvement of soil microbial efficiency, especially at the maize seedling season. Keywords: basal respiration, metabolic quotient, microbial biomass, seasonal variation, soil organic carbon Journal: Plant, Soil and Environment Pages: 511-516 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/446/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/446/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0005.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:446-2013-PSE Template-Type: ReDIF-Article 1.0 Author-Name: L. Vitale Author-Workplace-Name: National Research Council - Institute for Mediterranean Agriculture and Forest Systems (CNR-ISAFoM), Ercolano, Italy Author-Name: L. Ottaiano Author-Workplace-Name: Department of Agronomy, Naples University Federico II, Portici, Italy Author-Name: F. Polimeno Author-Workplace-Name: National Research Council - Institute for Animal Production System in Mediterranean Environment (CNR-ISPAAM), Naples, Italy Author-Name: G. Maglione Author-Workplace-Name: National Research Council - Institute for Animal Production System in Mediterranean Environment (CNR-ISPAAM), Naples, Italy Author-Name: U. Amato Author-Workplace-Name: National Research Council ? Institute for Applied Mathematics ?Mauro Picone?, Naples, Italy Author-Name: C. Arena Author-Workplace-Name: Deparment of Biology, Naples University Federico II, Naples, Italy Author-Name: P. Di Tommasi Author-Workplace-Name: National Research Council - Institute for Mediterranean Agriculture and Forest Systems (CNR-ISAFoM), Ercolano, Italy Author-Name: M. Mori Author-Workplace-Name: Department of Agronomy, Naples University Federico II, Portici, Italy Author-Name: V. Magliulo Author-Workplace-Name: National Research Council - Institute for Mediterranean Agriculture and Forest Systems (CNR-ISAFoM), Ercolano, Italy Title: Effects of 3,4-dimethylphyrazole phosphate-added nitrogen fertilizers on crop growth and N2O emissions in Southern Italy Abstract: The effect of the nitrification inhibitor 3,4-dimethylphyrazole phosphate (DMPP) on N-fertilized crop growth and soil N2O emissions were studied at two experimental sites in Southern Italy, characterised by a Mediterranean climate and different soil texture. The experiments were a randomized block design of two treatments: crop fertilized with NH4NO3 (considered the control treatment) or amended with DMPP plus NH4NO3 (considered the DMPP treatment). ANOVA was performed to assess differences between treatments and fertilization periods whereas simple and multiple linear regressions were performed in order to assess the effect of the soil-related in-dependent variables on soil gases emissions. Growth of potato plants fertilized with DMPP-added nitrogen was enhanced compared to control plants, whereas no benefit on maize plants grown during summer was observed. N2O emissions measured from soil to potato after the first fertilization with DMPP-added nitrogen was reduced during winter, but was higher than control after the second fertilizer application in spring, leading to comparable N2O emission factors (EF1) between treatments. In maize N2O emissions and EF1 were lower for DMPP compared to control treatment. The effectiveness of reduction in soil N2O emission was influenced by soil temperature and water-filled pore space (WFPS) in both experimental sites. However, the overall effect of WFPS was contrasting as N2O emissions were decreased in potato and enhanced in maize. Keywords: greenhouse gases, plant growth, nitrous oxide, DMPP, Mediterranean climate Journal: Plant, Soil and Environment Pages: 517-523 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/362/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/362/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0006.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:362-2013-PSE Template-Type: ReDIF-Article 1.0 Author-Name: Z. Guo Author-Workplace-Name: Soiland Fertilizer Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui, P.R. China Author-Workplace-Name: Key Laboratory of Nutrient Cycling and Resources Environment of AnHui Province, Author-Name: X. Guo Author-Workplace-Name: Soiland Fertilizer Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui, P.R. China Author-Workplace-Name: Key Laboratory of Nutrient Cycling and Resources Environment of AnHui Province, Author-Name: J. Wang Author-Workplace-Name: Soiland Fertilizer Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui, P.R. China Author-Workplace-Name: Key Laboratory of Nutrient Cycling and Resources Environment of AnHui Province, Author-Name: D. Wang Author-Workplace-Name: Soiland Fertilizer Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui, P.R. China Author-Workplace-Name: Key Laboratory of Nutrient Cycling and Resources Environment of AnHui Province, Title: Occlusive effect of soil aggregates on increased soil DTPA-extractable zinc under low soil pH causedby long-term fertilization Abstract: To investigate the effect of low soil pH caused by fertilization on soil available zinc in calcareous soil, this study was conducted based on a long-term experiment consisting of: (a) no fertilization (CT); (b) mineral fertilizer application coupled with 7500 kg/ha of wheat straw (WS-NPK); (c) mineral fertilizer application coupled with 3750 kg/ha of wheat straw (1/2WS-NPK); (d) mineral fertilizer application alone (NPK). Long-term fertilization results in a significant increase in soil DTPA-extractable zinc. However, the increased soil DTPA-extractable zinc is unavailable to crops and mainly confined to 0.25 mm > and 0.25 mm to 1 mm aggregates. Compared to CT, soil DTPA-extractable zinc under fertilization is more than 9.67% and 122.36% higher in 0.25 mm > and 0.25 mm to 1 mm aggregates, respectively. Furthermore, plant-available zinc in the 0-15 cm soil layer and wheat grain zinc are both significantly positive related to soil DTPA-extractable zinc in > 2 mm aggregates. Therefore, plant-available zinc in the 0-15 cm layer is closely associated with DTPA-extractable zinc in > 2 mm aggregates, and the low soil pH caused by long-term fertilization could not enhance plant-available zinc in the surface soil layer nor elevate wheat grain zinc concentration because of the occlusive effect of soil aggregates. Keywords: soil total zinc, soil organic carbon, wheat straw, grain zinc conce Journal: Plant, Soil and Environment Pages: 524-529 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/489/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/489/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0007.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:489-2013-PSE Template-Type: ReDIF-Article 1.0 Author-Name: S. Wang Author-Workplace-Name: Institute of Soil and Fertilizer and Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, P.R. China/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture, P.R. China/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, P.R. China Author-Name: X. Liang Author-Workplace-Name: Institute of Environmental Science and Technology, College of Environmental and Resources Sciences, Zhejiang University, Hangzhou, P.R. China Author-Name: G. Liu Author-Workplace-Name: Institute of Soil and Fertilizer and Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, P.R. China/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture, P.R. China/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, P.R. China Author-Name: H. Li Author-Workplace-Name: Zhejiang Academy of Agricultural Sciences, Hangzhou, P.R. China Author-Name: X. Liu Author-Workplace-Name: Institute of Soil and Fertilizer and Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, P.R. China/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture, P.R. China/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, P.R. China Author-Name: F. Fan Author-Workplace-Name: Institute of Soil and Fertilizer and Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, P.R. China/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture, P.R. China/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, P.R. China Author-Name: W. Xia Author-Workplace-Name: Institute of Soil and Fertilizer and Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, P.R. China/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture, P.R. China/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, P.R. China Author-Name: P. Wang Author-Workplace-Name: Institute of Soil and Fertilizer and Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, P.R. China/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture, P.R. China/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, P.R. China Author-Name: Y. Ye Author-Workplace-Name: Institute of Environmental Science and Technology, College of Environmental and Resources Sciences, Zhejiang University, Hangzhou, P.R. China Author-Name: L. Li Author-Workplace-Name: Institute of Environmental Science and Technology, College of Environmental and Resources Sciences, Zhejiang University, Hangzhou, P.R. China Author-Name: Z. Liu Author-Workplace-Name: Institute of Soil and Fertilizer and Resources and Environment, Jiangxi Academy of Agricultural Sciences/Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, P.R. China/Key Laboratory of Crop Ecophysiology and Farming System for the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture, P.R. China/National Engineering and Technology Research Center for Red Soil Improvement, Nanchang, P.R. China Author-Name: J. Zhu Author-Workplace-Name: Renewable Energy and Environmental Engineering, Department of Bioproducts and Biosystems Engineering, Southern Research and Outreach Center, University of Minnesota, Waseca, USA Title: Phosphorus loss potential and phosphatase activities in paddy soils Abstract: The effects of phosphorus (P) fertilizer on P loss potential, soil Olsen-P and neutral phosphatase activities in paddy soils fertilized with superphosphate or pig manure (PM) were evaluated in this paper. Data were collected from a field experiment in the Tai Lake Basin, China. Superphosphate rates were 0, 17.5, 26.7, and 35.0 kg P/ha, and PM rates were 0, 1.4, 2.1, and 2.8 t/ha for each crop, respectively. Soil Olsen-P in the plow layer increased to a greater extent with PM than with superphosphate. Pig manure increased neutral phosphatase activities in the plow layer compared with PM-free treatment. In contrast, superphosphate inhibited neutral phosphatase activities compared with superphosphate-free treatment. Spring application of P fertilizer markedly increased the total P of surface water in November (< 0.01 vs. 0.10 mg/L) compared with P-free treatment. The total P of shallow groundwater at a 75 cm depth was ~0.01 mg/L. Phosphorus fertilizer did not influence Olsen-P or neutral phosphatase activities under the plow layer. Downward movement of P did not occur. Appropriate rate of P application of 26.2 kg P/ha for each crop in this soil reduced the risk of P loss in the paddy wetland ecosystem. Keywords: soil Olsen-P, neutral phosphatase activity, water total phosphorus, loss potential, paddy wetland ecosystem Journal: Plant, Soil and Environment Pages: 530-536 Volume: 59 Issue: 11 Year: 2013 DOI: 10.17221/626/2013-PSE File-URL: http://pse.agriculturejournals.cz/doi/10.17221/626/2013-PSE.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/pse-201311-0008.txt Handle: RePEc:caa:jnlpse:v:59:y:2013:i:11:id:626-2013-PSE