Template-Type: ReDIF-Article 1.0 Author-Name: I. Herzig Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: B. Pisarikova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Kursa Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Bendova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Effects of humine compounds on iodine utilisation and retention and on the function of the thyroid gland Abstract: Effects of sodium humate (HuNa), containing 61.9% of humic acids in dry matter, on utilisation and retention of dietary iodine were investigated in balance experiments carried out in pigs. A control group was fed a commercial diet containing 0.1 mg of iodine per kg. The daily intake of iodine was 129.2 µg per animal or 4.08 µg per kg live weight. The recommended intake of dietary iodine is 0.25 to 0.30 mg per kg at 88% dry matter. The experimental group (HuNa) was fed the same diet supplemented with 3% of sodium humate which contained 0.5 mg of iodine per kg at 90.04% dry matter. The supplementation increased the daily intake to 141.4 µg per animal, or 4.45 µg per kg live weight. The supplementation of HuNa increased significantly the amount of iodine excreted in faeces (53.2 ± 11.0 vs. 43.0 ± 6.47 µg per animal per day; P < 0.05) and nonsignificantly the urinary iodine excretion (31.8 ± 8.93 vs. 29.0 ± 11.3 µg per animal per day). Differences between the HuNa and the control groups in blood serum iodine concentrations (18.8 vs. 18.1 µg per litre) and urinary iodine concentrations (32.5 vs. 34.4 µg per litre) were nonsignificant. It is evident from the concentrations that the pigs suffered from a serious iodine deficiency. Compared with controls, the HuNa group showed significantly higher triiodothyronine concentration (P < 0.05) and nonsignificantly lower thyroxine and iodine concentrations in blood serum. Dietary iodine utilisation was lower in the HuNa than in the control group (62.3 vs. 66.6%). The results show that utilisation of dietary iodine was limited and that sodium humate (humic acids) reduced iodine utilisation and retention and affected also other parameters indicating their goitrogenic effects. Keywords: swine, humine acids, triiodothyronine, thyroxine, urine, faeces Journal: Veterinární medicína Pages: 61-64 Volume: 46 Issue: 3 Year: 2001 DOI: 10.17221/7855-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7855-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200103-0001.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:3:id:7855-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Vajner Author-Workplace-Name: Institute of Histology and Embryology, Author-Name: V. Konrádová Author-Workplace-Name: Institute of Histology and Embryology, Author-Name: J. Uhlík Author-Workplace-Name: Institute of Histology and Embryology, Author-Name: J. Zocová Author-Workplace-Name: Institute of Histology and Embryology, Title: The effect of oral administration of salbutamol on the glycoconjugate composition in goblet cells of the tracheal epithelium in rabbits Abstract: We verified the influence of salbutamol on changes of glycoconjugates contained in tracheal goblet cells in rabbits by the oral administration of Ventolin™ syrup in the dose of 5 ml. Material for both conventional and lectin histochemistry was collected 15 and 30 minutes post exposure. Gradual decrease of percentage of goblet cells containing acid sialylated glycoconjugates was observed 15 minutes after administration. Thirty minutes after administration, neutral glycoconjugates-containing goblet cells were absent. The proportion of goblet cells containing acid sialylated glycoconjugates reached 50% of the value in control animals. Compared with controls, the changes of the character of the glycoconjugate content in the tracheal goblet cells due to the oral administration of Ventolin™ syrup were statistically significant ((α ≤ 0.01). Keywords: tracheal epithelium, goblet cells, sialylated glycoconjugates, lectin histochemistry, rabbit, Ventolin syrup Journal: Veterinární medicína Pages: 65-69 Volume: 46 Issue: 3 Year: 2001 DOI: 10.17221/7854-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7854-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200103-0002.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:3:id:7854-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Vajner Author-Workplace-Name: Institute of Histology and Embryology, 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: V. Vortel Author-Workplace-Name: Institute of Histology and Embryology, 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: A. Brejcha Author-Workplace-Name: Institute of Histology and Embryology, 2nd Medical Faculty, Charles University, Prague, Czech Republic Title: The expression of sialylated molecules in parafollicular-cell hyperplasia of the canine thyroid gland Abstract: : During the 18-year period (1974-1991), the lymphocytic thyroiditis with the finding of serum autoantibodies against thyroglobulin was diagnosed in 180 Beagle dogs (92 males and 88 females). In 107 of them (56 males and 51 females), hyperplasia of parafollicular cells was concurrently encountered. In further 11 cases (3 males and 8 females), solid cellular structures were found in the thyroid parenchyma, in 4 females combined with unilocular or multilocular lymphoepithelial cysts. Grimelius stain revealed the presence of parafollicular cells even at the periphery of cellular nests. Using the lectin histochemistry with Maackia amurensis agglutinin (MAA),Sambucus nigra agglutinin (SNA) and Tritrichomonas mobilensis lectin (TML), the presence of sialylated molecules was demonstrated in the cell membranes and perinuclear cytoplasmic regions of parafollicular cells that formed hyperplastic nodules or were interspersed in "solid cell nests". Keywords: Beagle dog, C-cell hyperplasia, solid cell nests, thyroid, sialylation, lectin histochemistry Journal: Veterinární medicína Pages: 70-74 Volume: 46 Issue: 3 Year: 2001 DOI: 10.17221/7856-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7856-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200103-0003.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:3:id:7856-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Fortýn Author-Workplace-Name: Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libìchov, Czech Republic Author-Name: V. Hruban Author-Workplace-Name: Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libìchov, Czech Republic Author-Name: V. Horák Author-Workplace-Name: Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libìchov, Czech Republic Author-Name: J. Tichý Author-Workplace-Name: Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libìchov, Czech Republic Title: Artificial perforations of the stomach in pigs and rats and their covering by the abdominal wall Abstract: The stitching of artificially created opening in the stomach to the parietal peritoneum has been experimentally examined so that the missing resected part was covered with the abdominal wall. Stomach contents were in direct contact with the peritoneal surface. In contradiction to common opinions (the rise of peritonitis and subsequent death) all experimental animals (six pigs and ten rats) survived the operation without any apparent complications. Within eight weeks following the laparotomy ("a second look"), the artificial perforation was narrowed down partly with outgrown gastric mucosa and partly with new generated fibrous tissue. The results of this strange experiment turn down common convictions about development of generalised peritonitis. The experiments are not an end in itself but offer possible use in a clinical experiment in humans. A casuistic of one patient with the perforated ulcer-carcinoma is discussed in this report. Keywords: pig, rat, stomach, artificial perforation, surgical intervention Journal: Veterinární medicína Pages: 75-77 Volume: 46 Issue: 3 Year: 2001 DOI: 10.17221/7857-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7857-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200103-0004.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:3:id:7857-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Rajchard Author-Workplace-Name: University of South Bohemia, Faculty of Agriculture, Èeské Budìjovice, Czech Republic Author-Name: V. Rachač Author-Workplace-Name: University of South Bohemia, Faculty of Agriculture, Èeské Budìjovice, Czech Republic Title: Find of bird-pox (variola avium) in blackcap (Sylvia atricapilla) Abstract: In August 1999 there were netted and ringed passerines in the Naděje fishpond system in Třeboň area. 14 individuals of blackcap (Sylvia atricapilla) of both sexes and various age were caught. All individuals of this species had expressive pathological changes on feet. Histological investigations proved bird-pox (variola avium). No symptoms of this disease were found in other caught 11 species. Keywords: blackcap (Sylvia atricapilla), bird-pox, skin form Journal: Veterinární medicína Pages: 78-79 Volume: 46 Issue: 3 Year: 2001 DOI: 10.17221/7858-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7858-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200103-0005.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:3:id:7858-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Hruška Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Czech research in veterinary medicine Abstract: The task of applied research is not only to acquire new knowledge, through which it contributes to the development of economy, to the consumer protection or to the training of qualified experts. For state-supported veterinary research, this means striving to protect people's health against diseases transmitted from animals, food-born diseases, contamination of feed and foodstuffs by toxic xenobiotics, a reduction in food quality through the use of low-grade raw materials and an adverse effect of environmental pollution. The territory of the state must be protected against the infectious diseases and animals against the negative effects of environment and technologies which often strive irresponsibly for the highest profits without regard for the consequences. The contributions of the Veterinary Research Institute, Brno, and other Czech research facilities to increasing knowledge on cattle health and reproduction are documented by the list of 105 references of papers published in peer reviewed journals since 1992. This article is available on http://www.vri.cz/vetmed.asp and the reprints of cited references can be requested by e-mail to <vri@vri.cz> Keywords: research, veterinary, biotechnology, epidemiology, CENTAUR network, cattle, paratuberculosis, toxicology, environment, pollution Journal: Veterinární medicína Pages: 80-87 Volume: 46 Issue: 3 Year: 2001 DOI: 10.17221/7859-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7859-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200103-0006.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:3:id:7859-VETMED