Template-Type: ReDIF-Article 1.0 Author-Name: S. Reschová Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: D. Pokorová Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Nevoránková Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Franz Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Monoclonal antibodies to bovine coronavirus and their use in enzymoimmunoanalysis and immunochromatography Abstract: Two monoclonal antibodies (MAb) to the outer structural protein E2 (spike peplomeric protein) and two MAb to the inner capsid protein N of bovine coronavirus (BCV) were prepared and identified by Western blotting to be used for increasing the specificity and sensitivity of BCV detection. The MAb were checked by the haemagglutination inhibition test and immunoperoxidase tests and no cross reactivity with rotavirus was demonstrated by the immunoperoxidase test and ELISA. A mixture of all the four MAb at predetermined optimum concentrations was first used in sandwich ELISA and then, in combination with an anti‑coronavirus polyclonal antibody, for the development of a simple and rapid immunochromatographic test (ICT). The results of which can be read visually within 10 min. The inclusion of MAb into ELISA and ICT allows the detection of both intact and incomplete BCV virions. ELISA and ICT were used in the examination of a set of 74 faecal samples collected from calves suffering from diarrhoea. ELISA, used as the golden standard verified by electron microscopy, detected BCV in 15 samples (20.3%) and ICT in 16 samples. Three of the ICT‑positive samples were negative by ELISA. On the other hand, two of the 58 ICT‑negative samples were positive by ELISA. Sensitivity and specificity of ICT were 94.9% and 86.7%, respectively Keywords: Immunochromatographic test (ICT), ELISA, bovine rotavirus, bovine coronavirus (BCV) Journal: Veterinární medicína Pages: 125-131 Volume: 46 Issue: 5 Year: 2001 DOI: 10.17221/7869-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7869-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200105-0001.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:5:id:7869-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Ulrich Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Raszyk Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A. Nápravník Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Variations in contamination by mercury, cadmium and lead on swine farms in the district of Hodonin in 1994 to 1999 Abstract: Environmental samples (n = 254) were collected at regular intervals from 1994 to 1999 on three swine farms in the area of the district town Hodonín, Czech Republic. The samples of feed mixtures (n = 85), barn dust sediments (n = 44), and muscular (n = 42), hepatic (n = 41), and renal (n = 42) tissues of feeder pigs were analysed for the concentrations of mercury, cadmium and lead. The concentrations of mercury showed decreasing tendencies in animal tissue samples (1994-1996). Compared with the mean for the Czech Republic, the concentration of mercury was markedly higher in liver and kidney samples (0.064 mg/kg and 0.114 mg/kg, respectively) collected in 1998, the concentration of cadmium was moderately higher in feed, muscle, liver, and kidney samples collected in 1996-1998. Statutory limits were exceeded in two liver and two kidney samples and one feed sample only. The concentration of lead in muscle, kidney, and feed samples (1994-1996) and barn dust samples (1996-1998) showed also a decreasing tendency. However, the limit was exceeded in 16 muscle samples (0.11 to 0.23 mg/kg) collected in 1994-1999 and two kidney samples collected in 1996 (0.74 mg/kg) and 1999 (0.77 mg/kg). Heavy metals present in dust sediments apparently did not contribute significantly to their content in animal tissue. While the health risk resulting from the contents of mercury and cadmium can be classified as moderate, the contents of lead must be regarded as markedly more dangerous. Keywords: swine farms, heavy metals, mercury, cadmium, lead, feed mixtures, dust sediments, pork, liver, kidney, health risk Journal: Veterinární medicína Pages: 132-139 Volume: 46 Issue: 5 Year: 2001 DOI: 10.17221/7872-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7872-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200105-0002.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:5:id:7872-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Sahin Author-Workplace-Name: Department of Animal Nutrition, Veterinary Faculty, University of Firat, Elazig, Turkey Author-Name: N. Sahin Author-Workplace-Name: Department of Animal Nutrition, Veterinary Faculty, University of Firat, Elazig, Turkey Author-Name: M. Onderci Author-Workplace-Name: Department of Animal Nutrition, Veterinary Faculty, University of Firat, Elazig, Turkey Author-Name: S. Yaralioglu Author-Workplace-Name: Department of Animal Nutrition, Veterinary Faculty, University of Firat, Elazig, Turkey Author-Name: O. Kucuk Author-Workplace-Name: Department of Animal Nutrition, Veterinary Faculty, University of Firat, Elazig, Turkey Title: Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress Abstract: An experiment utilizing Cobb-500 male broilers was conducted to evaluate the effects of vitamin E supplementation at various concentrations on malonyldialdehyde (MDA) as an indicator of lipid peroxidation, serum and liver concentrations of antioxidant vitamins and some minerals of broilers reared under heat stress (32°C). One day-old 150 male broilers were randomly assigned to 5 treatment groups, 3 replicates of 10 birds each. The birds received either a basal diet or basal diet supplemented with vitamin E (dl-a-tocopherol acetate) at 62.5, 125, 250, or 500 mg/kg of diet. Increased supplemental vitamin E linearly increased serum vitamin E and A, but decreased (P = 0.001) MDA concentrations. Increasing dietary vitamin E supplementation also resulted in linear increases in liver vitamin E and A concentrations, but linear decreases in MDA concentrations (P = 0.01). Increasing dietary vitamin E caused a linear increase in serum concentrations of Fe and Zn (P= 0.001), but a decrease in serum concentration of Cu (P = 0.001). Results of the present study conclude that in broiler chicks reared under heat stress a 250 mg of vitamin E supplementation can be considered as a protective management practice in a broiler diet, reducing the negative effects of heat stress. Keywords: malonyldialdehyde, vitamin A, vitamin E, Fe, Zn, Cu Journal: Veterinární medicína Pages: 140-144 Volume: 46 Issue: 5 Year: 2001 DOI: 10.17221/7870-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7870-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200105-0003.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:5:id:7870-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Mitak Author-Workplace-Name: Croatian Veterinary Institute, Department of Immunology, Zagreb, Croatia Author-Name: T. Gojmerac Author-Workplace-Name: Croatian Veterinary Institute, Department of Immunology, Zagreb, Croatia Author-Name: B. Mandić Author-Workplace-Name: Croatian Veterinary Institute, Department of Immunology, Zagreb, Croatia Author-Name: Ž. Cvetnić Author-Workplace-Name: Croatian Veterinary Institute, Department of Immunology, Zagreb, Croatia Title: Changes in serum concentration of 17 beta-estradiol in female rats during estrous cycle after treatment with atrazine and zearalenone Abstract: A daily dose of 14 mg atrazine and 2.5 mg zearalenone, given p.o. during 5 days of estrous cycle to female rats, changed their estrous cycle in comparison with control animals. On day -1 of expected estrus, a significantly lower (p < 0.05) concentration of 17b-estradiol compared with the control group was recorded in all experimental groups of animals. In the group of animals administered zearalenone, the concentration of 17b-estradiol on the day of expected estrus was significantly higher (p < 0.05). In the group administered a combination of atrazine and zearalenone, the concentration of 17b-estradiol on the day after expected estrus was significantly higher (p < 0.05) than in the control group. In the group of animals receiving atrazine, complete absence of the onset of estrous cycle was recorded, whereas in the group given zearalenone the onset of estrous cycle was delayed by 24 hours. The combination of atrazine and zearalenone induced similar effects as atrazine, however, with the onset of estrous cycle being delayed by 48 hours. Neither of these two groups of animals reached the level of 17b-estradiol recorded in the control group. Keywords: atrazine, zearalenone, estrous cycle, 17b-estradiol, serum, rat Journal: Veterinární medicína Pages: 145-148 Volume: 46 Issue: 5 Year: 2001 DOI: 10.17221/7868-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7868-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200105-0004.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:5:id:7868-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Stosik Author-Workplace-Name: Higher Pedagogical School, Zielona Góra, Poland Author-Name: W. Deptuła Author-Workplace-Name: Higher Pedagogical School, Zielona Góra, Poland Author-Name: K. Wiktorowicz Author-Workplace-Name: Higher Pedagogical School, Zielona Góra, Poland Author-Name: M. Trávniček Author-Workplace-Name: Higher Pedagogical School, Zielona Góra, Poland Author-Name: K. Baldy-Chudzik Author-Workplace-Name: Higher Pedagogical School, Zielona Góra, Poland Title: Qualitative and quantitative cytometric analysis of peripheral blood leukocytes in carps (Cyprinus carpio) Abstract: The studies were performed involving qualitative and quantitative cytometric analysis of peripheral blood leukocytes in healthy carps at various stages of their ontogeny, i.e., in 3- to 29-month old carps. Three populations of leukocyte line cells were distinguished, which differed in forward scatter (FSC) and side scatter (90o, SSC) of laser light. The most abundant leukocyte pool was present in the eldest (23- to 29-month old) fish. Lower numbers of the cells were observed in the youngest (3- to 9-month old) carps while the lowest levels of the cells were detected in carps of the moderate age (11- to 21-month old). The leukocyte populations, distinguished on grounds of FSC/SSC characteristics, were suggested to correspond to lymphocytes/thrombocytes (low FSC, low SSC), granulocytes (high FSC, high SSC) and monocytes (high FSC, low SSC). Keywords: carp, leukocytes, cytometry Journal: Veterinární medicína Pages: 149-152 Volume: 46 Issue: 5 Year: 2001 DOI: 10.17221/7871-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7871-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200105-0005.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:5:id:7871-VETMED