Template-Type: ReDIF-Article 1.0 Author-Name: I. Herzig Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic. Author-Name: B. Písaříková Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic. Author-Name: I. Diblíková Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic. Author-Name: P. Suchý Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic. Title: Iodine concentrations in porcine blood, urine, and tissues after a single dose of iodised oil Abstract: Experimental groups of pigs were treated orally with 120 mg (Group O 120), or 480 mg (Group O 480) of iodine per animal, or intramuscularly with 240 mg (Group I 240) of iodine per animal. Iodine was administered in the form of iodised fatty acid esters (IFAE). The treatment resulted in significantly increased iodine concentrations in tissues and a single dose was sufficient to meet the requirement for the whole fattening period (180 days). Urinary iodine concentrations in all the experimental groups were higher than in the control group C receiving iodine only from conventional feed. Urinary excretion of iodine between days 2 and 5 was more distinctive in orally treated than in intramuscularly treated animals (Figure 1). Iodine concentrations at the end of the fattening period (day 180) were higher in the treated than in the control groups. The treatment effect was more marked in Groups O 480 and I 240 than in Group O 120. The dynamics of blood serum iodine concentrations was similar to urinary concentrations (Figure 2). Mean thyroid gland weights in the groups O 120, O 480, I 240, and C were 9.19, 8.51, 7.10, and 12.01 g, respectively. An opposite tendency was observed for iodine concentrations in thyroid gland dry matter (Figure 3). No effects of any of the treatments on total protein, albumin, total lipids, or cholesterol concentrations in blood serum were observed. Group C showed lower tissue iodine concentrations than any of the experimental groups. The only exception was hepatic tissue in which approximately the same iodine concentrations were found in all the groups. Data obtained in Groups O 120, O 480, and I 240 indicate that decisive for tissue concentrations was rather the dose of iodine than the route of administration. Iodine is stored above all in the thyroid gland and adipose tissue. As can be seen in Figure 4, its concentration was higher in muscles with a higher proportion of fat (neck) than in lean muscles (ham). Keywords: Lipiodol® UF, muscular and lipid tissues, thyroid gland, triiodothyronine, thyroxine, total protein, albumin, total lipids, cholestero Journal: Veterinární medicína Pages: 153-159 Volume: 46 Issue: 6 Year: 2001 DOI: 10.17221/7875-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7875-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200106-0001.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:6:id:7875-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Faldíková Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Diblíková Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Čanderle Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Zralý Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Věžník Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A. Šulcová Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Effects of nutrition, social factors and chronic stress on the mouse Leydig cell testosterone production Abstract: The testosterone production by Leydig cells of mice adversely affected with nutritional or social factors and exposed to chronic stress was studied in vitro. Both basal and gonadotrophin stimulated testosterone production were highly significantly decreased (P < 0.01) as in groups given the hypothyreotics or potassium nitrate alone as in combination with stress. Stimulated in vitro testosterone production was not significantly changed in groups fed with synthetic diets, however, in mice given modified diets and exposed to stress decreased responsivity of Leydig cells stimulated by gonadotrophin was found. Basal and stimulated testosterone production in vitro in most of gonadotrophin concentrations was non-significantly lower in mice affected only by stress when compared with undisturbed controls. In isolated male mice the basal testosterone production was significantly (P < 0.05) decreased and gonadotrophin stimulated production highly significantly (P < 0.01) decreased when compared with group caged males. The testosterone production was most severely suppressed in aggressive individuals. Serum testosterone levels were detected in all animals, corticosterone, T3and T4 in selected groups of mice. We can conclude that the testosterone production was adversely affected by nutritional factors, and the impact was more profound when exerted together with chronic stress. The adverse effect of individual caging of male mice was also proved. Keywords: mice, chronic stress, hypothryeosis, potassium nitrate, fat-free diet, individual caging, aggressive and timid behaviour, Leydig cells in vitro, gonadotrophin, testosterone Journal: Veterinární medicína Pages: 160-168 Volume: 46 Issue: 6 Year: 2001 DOI: 10.17221/7874-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7874-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200106-0002.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:6:id:7874-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Sedmíková Author-Workplace-Name: Department of Veterinary Science, Czech University of Agriculture, Prague, Czech Republic Author-Name: H. Reisnerová Author-Workplace-Name: Department of Veterinary Science, Czech University of Agriculture, Prague, Czech Republic Author-Name: Z. Dufková Author-Workplace-Name: Department of Veterinary Science, Czech University of Agriculture, Prague, Czech Republic Author-Name: I. Bárta Author-Workplace-Name: Department of Veterinary Science, Czech University of Agriculture, Prague, Czech Republic Author-Name: F. Jílek Author-Workplace-Name: Department of Veterinary Science, Czech University of Agriculture, Prague, Czech Republic Title: Potential hazard of simultaneous occurrence of aflatoxin B-1 and ochratoxin A Abstract: Contents of aflatoxin B1 (AFB1) and ochratoxin A (OA) in the samples of wheat and barley were detected by RIA method. Average contents of AFB1 in the samples of wheat and barley were 2.4 µg/kg and 2.2 µg/kg, respectively. Average contents of OA in the samples of wheat and barley were 3.1 µg/kg and 2.4 µg/kg, respectively. The contents of mycotoxins corresponded to the allowed limits in food. During the studies of mutagenic activity of mycotoxins and their combinations by means of Ames test it was found that OA could increase the mutagenicity of AFB1 in the case of their simultaneous occurrence in the same substrate. Keywords: mycotoxins, residua, cereals, Ames test, mutagenicity Journal: Veterinární medicína Pages: 169-171 Volume: 46 Issue: 6 Year: 2001 DOI: 10.17221/7876-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7876-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200106-0003.txt Handle: RePEc:caa:jnlvet:v:46:y:2001:i:6:id:7876-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V.V. Bajt Author-Workplace-Name: Zagreb University, Veterinary Faculty, Department of Humanities, Chair of Veterinary Medicine History, Zagreb, Croatia Author-Name: G.G. Gračner Author-Workplace-Name: Zagreb University, Veterinary Faculty, Department of Humanities, Chair of Veterinary Medicine History, Zagreb, Croatia Author-Name: A. Škrobonja Author-Workplace-Name: Zagreb University, Veterinary Faculty, Department of Humanities, Chair of Veterinary Medicine History, Zagreb, Croatia Title: Professor Josip Ubl's contribution to the development of veterinary medicine in Croatia Abstract: Numerous data point to the fact that Czech people played an important role in the cultural development of Croatia. Professor Josip Ubl was one of the many outstanding Czech veterinarians who greatly contributed to the Croatian veterinary medicine. Prof. Josip Ubl was born on the 4th April 1844 in Chudenice, in the Plzeň district. He descended from a respectable family, which highly influenced his schooling. He finished his primary and secondary education at his birthplace. He graduated from the k. u. k. Militär-Thierartznei-Institute in Vienna in 1867. Prof. Josip Ubl first worked as an assistant lecturer and as a professor of animal husbandry and veterinary medicine at the School of Farming and Farming Crafts in Doubravice near Loštice and Mohelnice in Moravia. Later on he was appointed a teacher of veterinary medicine, animal husbandry, anatomy and zoology at the Kraljevsko gospodarsko-šumarsko učilište i ratarnica (Royal School of Farming and Forestry) in Križevci in Croatia. He was an exceptionally prolific writer and wrote seven veterinary manuals. Besides being an outstanding teacher he was also engaged in social work and was awarded for his contribution to this field of work several times. As the author of the first veterinary works in Croatian language and the creator of the Croatian veterinary terminology he has gained a prominent place in the history of Croatian veterinary medicine. Keywords: Josip Ubl, Croatia, history of veterinary medicine Journal: Veterinární medicína Pages: 175-180 Volume: 46 Issue: 6 Year: 2001 DOI: 10.17221/7873-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/7873-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:46:y:2001:i:6:id:7873-VETMED