Template-Type: ReDIF-Article 1.0 Author-Name: V. Rada Author-Workplace-Name: Department of Microbiology and Biotechnology, Czech University of Agriculture, Prague, Czech Republic Author-Name: J. Petr Author-Workplace-Name: Department of Microbiology and Biotechnology, Czech University of Agriculture, Prague, Czech Republic Title: Enumeration of bifidobacteria in animal intestina samples Abstract: TPY agar supplemented with mupirocin (100 mg/liter) and glacial acetic acid (1 ml/liter) was effective in the enumeration of bifidobacteria in animal (11 species) fecal or intestinal samples. On the other hand, less than 50% of isolates from pig feces were identified as Bifidobacterium sp. using this medium. Subsequently TPY agar modified by the addition of mupirocin (100 mg/liter), glacial acetic acid (1 ml/liter), and colistin (25 mg/liter) was developed for the enumeration of bifidobacteria in pig feces. The results suggest that the bifidobacteria selective media should be chosen in respect of the animal species origin of the samples tested. Keywords: bifidobacteria, selective media, intestinal tract, reces Journal: Veterinární medicína Pages: 1-4 Volume: 47 Issue: 1 Year: 2002 DOI: 10.17221/5794-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5794-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200201-0001.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5794-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Božič Author-Workplace-Name: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: L. Švec Author-Workplace-Name: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: I. Valpotič Author-Workplace-Name: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Zagreb, Croatia Title: CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis Abstract: Since no effective vaccine is available for its immunoprophylaxis, porcine post-weaning diarrhoea (PWD) induced by enterotoxigenic Escherichia coli (ETEC) strains remains an important cause of morbidity. Live attenuated oral vaccines have been suggested to be relatively effective in preventing ETEC-induced PWD in the pigs, but the mechanisms responsible for protection have not been elucidated. In the present study we have investigated the likely impact of oral vaccination of weaned pigs with non-ETEC strain expressing F4ac antigen on CD45RA and CD45RC isoforms expression on the surface of the mesenteric lymph node (MLN) cells and on spleen cells by using one-colour flow cytometry. Additionally, to assess the activation state of T cells in the MLN and spleen of the pigs, surface expression of the lymphocyte activation marker CD25 was analysed. Sham-vaccinated weaned pigs served as controls. Our results of the quantitative phenotypic analysis of isolated lymphocytes showed that the vaccinal E. coli strain induced elevation of both CD25+ and CD45RC+ cells in the spleen, but not MLN, of the vaccinated weaned pigs. This was accompanied by decreased CD45RA expression on spleen cells, suggesting that CD45RA+ spleen cells could develop CD45RC phenotype, probably as a consequence of activation in the vaccinated challenge-infected weaned pigs. Keywords: weaned pigs, vaccination, colibacillosis, CD45 isoforms, CD25, flow cytom Journal: Veterinární medicína Pages: 5-11 Volume: 47 Issue: 1 Year: 2002 DOI: 10.17221/5795-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5795-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200201-0002.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5795-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Mitak Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Author-Name: T. Gojmerac Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Author-Name: D. Cvrtila Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Author-Name: Ž. Cvetnič Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Title: Effect of atrazine and zearalenone on the numer of receptor binding sites for 3H-estradiol in the rat uterus cytosol Abstract: The effect of a daily dose of 14 mg atrazine and/or 2.5 mg zearalenone that were administered to rats during 5 days of estrous cycle on the number of receptor binding sites for 3H-estradiol in uterine cytosol was investigated. Comparison with a control group of animals showed no effect of atrazine influence on the number of receptor binding sites for 3H-estradiol in the rat uterine cytosol until day 6 of the experiment, when a significantly lower value was recorded (P < 0.05). In the group of animals administered zearalenone, a decline in the number of receptor binding sites for 3H-estradiol in uterine cytosol was observed. On day 4 of the experiment, the number of binding sites was significantly greater (P < 0.05) in this group than in the control group of animals, whereafter it gradually decreased to a significantly lower value (P < 0.05) as compared to the control group on day 6. In the group of animals administered a mixture of atrazine and zearalenone, the number of receptor binding sites for 3H-estradiol in uterine cytosol did not decrease but showed a similar pattern as in the control group of animals, however, at a statistically significantly (P < 0.05) higher level. Keywords: atrazine, zearalenone, 3H-estradiol receptor, ovary, uterus, rat Journal: Veterinární medicína Pages: 12-16 Volume: 47 Issue: 1 Year: 2002 DOI: 10.17221/5796-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5796-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200201-0003.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5796-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Stosik Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Author-Name: W. Deptula Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Author-Name: K. Wiktorowicz Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Author-Name: M. Trávníček Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Author-Name: K. Baldy-Chudzik Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Title: Respiratory burst in neutrophilic granulocytes of carps (Cyprinus carpio): cytometric studies Abstract: Present studies aimed at flow cytometric analysis of respiratory burst in neutrophilic granulocytes in carps, at various stages of their ontogeny. Cytometric evaluation of the repiratory burst in PMN cells of carps demonstrated augmented values of the mean fluorescence channel of PMA-stimulated PMN cells, as compared to the value of the fluorescence channel noted for PMN cells which were not activated or did not respond to activation in response to the applied stimulator. Intensity of the fluorescence, compared to the mean fluorescence channel of the cells which were not stimulated by PMA, was most pronounced in 11- to 21-month old carps, average in the youngest carps aging 3 to 9 months, and the lowest in the eldest, 23- to 29-month old carps. In cases of analysis of the mean fluorescence channel in the selected fraction of PMA-stimulated PMN cells, as compared to the mean fluorescence channel of granulocytes which did not respond by the stimulation reaction, the most intense fluorescence was noted to develop in the eldest, i.e. 23- to 29-month old fish. In the remaining carps, on the other hand, the difference in the mean fluorescence channel was significantly lower. In parallel, the highest fraction of PMA-stimulated granulocytes was found in 11- to 21-month old carps, a lower fraction in the youngest carps aging 3 to 9 months and the lowest fraction of such granulocytes in the eldest carps, 23 to 29 months of age. Keywords: carp, PMN cells, respiratory burst, citometry Journal: Veterinární medicína Pages: 17-20 Volume: 47 Issue: 1 Year: 2002 DOI: 10.17221/5797-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5797-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200201-0004.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5797-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Stosik Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Author-Name: W. Deptuła Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Author-Name: M. Trávniček Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Author-Name: K. Baldy-Chudzik Author-Workplace-Name: University of Zielona Góra, Zielona Góra, Poland Title: Phagocytic and bactericidal activity of blood thrombocytes in carps (Cyprinus carpio) Abstract: The studies aimed at monitoring development of phagocytic and bactericidal activity in carps in the course of their ontogenetic development. The studies were performed using the techniques described by Mantur et al. (1986a, b), adapted to fish. Results were expressed in thrombocyte phagocytic index (Ipt), percentage of phagocyting thrombocytes (%tp), percentage of ingested bacteria (%bp) and in the index of intracellular killing by thrombocytes (Ibt). Number of thrombocytes was also examined using the technique of Dessi. Results of testing thrombocyte capacity to ingest the standard Staphylococcus aureus 209P strain showed that Ipt and %tp increased gradually in carps between the age of 3 and 17 months. In older carps, 19 to 29 months of age, as well as in spawners the growth in activity paralelled the ontogenetic development within Ipt values while %tp values remained at a similar level. Phagocytic activity of thrombocytes expressed in Ipt was reflected also by %bp values, which was particularly evident in fish aging 23 months to 5 years. In the case of Ibt, values of the index were not related in any way to stage of ontogeny or sex of the fish. Number of thrombocytes in carps aged 3 months to 5 years was increasing in parallel to their ontogenetic development. Keywords: carp, thrombocytes, phagocytic ability, bactericidal aktivity Journal: Veterinární medicína Pages: 21-25 Volume: 47 Issue: 1 Year: 2002 DOI: 10.17221/5798-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5798-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200201-0005.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5798-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Kozák Author-Workplace-Name: Veterinary Administration of the City of Prague, Czech Republic Author-Name: V. Večerek Author-Workplace-Name: Veterinary Administration of the City of Prague, Czech Republic Author-Name: I. Steinhauserová Author-Workplace-Name: Veterinary Administration of the City of Prague, Czech Republic Author-Name: P. Chloupek Author-Workplace-Name: Veterinary Administration of the City of Prague, Czech Republic Author-Name: V. Pištěková Author-Workplace-Name: Veterinary Administration of the City of Prague, Czech Republic Title: Results of slaughterhouse carcass classification (capable for human consumption, capable for processing and condemned) in selected species of food animals Abstract: Numbers of food animal carcasses classified as capable for human consumption (edible), capable for processing (conditionally edible) and condemned belong among important parameters of health status of animals both on farm level and at slaughterhouses. The figures relating to these parameters were monitored at slaughterhouses during the periods of 1989 to 1994 and 1995 to 2000. Relative numbers of food animal carcasses classified as edible increased during the period of 1995 to 2000 in comparison to the period of 1989 to 1994 in different species as follows: in cows from 77.14 to 79.48%, in heifers from 83.06 to 92.49%, in bulls from 89.62 to 95.52%, in calves from 49.20 to 63.76%, in pigs from 95.13 to 97.27%, in sheep from 93.08 to 98.08%, in goats from 68.40 to 93.08%, in horses from 64.67 to 84.41%, in chickens from 97.06 to 98.48%, in hens from 94.41 to 95.16%, in turkeys from 97.41 to 98.00%, in ducks and geese from 98.70 to 99.13% and in rabbits from 83.95 to 93.64%. The increase was proven to be statistically significant in all species, production and age groups of food animals. It can be concluded that health status of food animals at slaughter has significantly improved during the recent period in all species, production and age groups included in the study. Best health status was currently found in chickens, ducks and geese, followed by turkeys, hens, sheep, pigs, bulls, rabbits, heifers and goats. On the other hand, poor health status was found in horses, cows and especially in calves. Keywords: food animals, capable for human consumption, capable for processing, condemned, health status, slaughterhouse Journal: Veterinární medicína Pages: 26-31 Volume: 47 Issue: 1 Year: 2002 DOI: 10.17221/5799-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5799-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200201-0006.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:1:id:5799-VETMED