Template-Type: ReDIF-Article 1.0 Author-Name: S. Čech Author-Workplace-Name: Department of Reproduction, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: V. Havlíček Author-Workplace-Name: Department of Reproduction, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Lopatářová Author-Workplace-Name: Department of Reproduction, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Vyskočil Author-Workplace-Name: Department of Reproduction, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Doležel Author-Workplace-Name: Department of Reproduction, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Effects of superstimulation with fsh on follicular population and recovery rate of oocytes in the growing phase of the first and second follicular wave Abstract: Effectiveness of in vitro production of embryos (IVP) is limited among other factors by the recovery rate of oocytes. Gonadotropin superstimulation can improve the recovery rate of oocytes. The effect of FSH treatment on follicular population and recovery rate of oocytes at ovum pick-up (OPU) in the growing phase of the 1st as well as the 2nd follicular wave after superstimulation was the object of our experiment. Twelve unpregnant milking cows (15-20 kg milk per day) housed on a dairy farm were used in the experiment. The cows bearing corpus luteum were synchronized by PGF2 (day 0) and they were treated by FSH (Folicotropin inj. sicc. ad us vet., Spofa Prague, Czech Republic, single doses 80, 80, 80, 80, 40 and 40 UI) in 12 h intervals on days 12, 13 and 14. Transvaginal ultrasonographic puncture of oocytes in cows bearing a new corpus luteum was performed on day 7 (OPU 1, various phase of the follicular wave, removal of the dominant follicle) and it was repeated on days 10 (OPU 2, growing phase of the follicular wave - control), 16 (OPU 3, growing phase of the first follicular wave after superstimulation) and 20 (OPU 4, growing phase of the second follicular wave after superstimulation). All follicles > 2 mm were punctured. The ovarian follicles (ultrasonographically) and numbers and qualities of obtained oocytes (microscopically) were evaluated during and immediately after OPU. Follicular population was divided to small (FS, 2-5 mm), medium (FM, 5-9 mm) and large (FL, > 9 mm) follicles. Oocytes were classified as 1st (intact cumulus, > 3 layers of cumulus cells), 2nd (complete 1-3 layers of cumulus cells), 3rd (incomplete layers of cumulus cells, expanded cumulus mass) and 4th (absence of corona cells, degenerated oocytes) classes. Although we found the least of FS (x = 1.0) during OPU 3, significantly more FM (x = 24.7) and FL (x = 3.1) follicles were found at this procedure in comparison with others. Likewise a significantly higher number of oocytes (x = 8.1) was obtained at OPU 3 in comparison with OPU 1 and OPU 2. Significantly higher number of FM (x = 6.1) was found and non-significantly higher number of oocytes was obtained at OPU 4 in comparison with OPU 1 and 2. The results show that administration of FSH increases the number of follicles and the number of collected oocytes in the growing phase of the 1st follicular wave after superstimulation, nevertheless a higher number of follicles and a higher recovery rate of oocytes can be expected in the growing phase of the 2nd follicular wave after superstimulation as well. Keywords: ovum pick-up, superstimulation, cattle Journal: Veterinární medicína Pages: 33-37 Volume: 47 Issue: 2-3 Year: 2002 DOI: 10.17221/5800-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5800-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200202-0001.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:2-3:id:5800-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Holovská Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Science, Košice, Slovak Republic Author-Name: V. Lenártová Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Science, Košice, Slovak Republic Author-Name: K. Holovská Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Science, Košice, Slovak Republic Author-Name: P. Javorský Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Science, Košice, Slovak Republic Title: Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis Abstract: Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium by Chelex 100, the addition of manganese increased enzymatic activity, while addition of iron inhibited SOD activity. Changes in Mn-SOD and glutathione peroxidase (GSHPx) activities evoked by paraquat and increased values of TBARS indicated that these enzymes were not able to sufficiently prevent oxidative stress at given paraquat concentrations. Keywords: Streptococcus bovis, paraquat, superoxide dismutase, glutathione peroxidase Journal: Veterinární medicína Pages: 38-44 Volume: 47 Issue: 2-3 Year: 2002 DOI: 10.17221/5801-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5801-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200202-0002.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:2-3:id:5801-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: W. Yayo Ayele Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: I. Parmova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: I. Melicharek Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: M. Hanzlikova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: B. Körmendy Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: G. Nagy Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: Z. Cvetnic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: M. Ocepek Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: N. Fejzic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: M. Lipiec Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Title: Incidence of bovine tuberculosis in cattle in seven Central European countries during the years 1990-1999 Abstract: The post-eradication incidence of bovine tuberculosis in seven Central European Countries (Bosnia and Herzegovina, Croatia, Czech Republic, Hungary, Poland, Slovakia and Slovenia) was studied between 1990 and 1999. The majority of cattle to the age of 24 months were screened by tuberculin skin test on annual basis. Tuberculous lesions observed during meat inspection at abattoirs were further laboratory examined by direct microscopy, cultivation and histology for the presence of mycobacteria. Data describing the incidence of the disease in animals for the whole period were obtained from all countries except Bosnia and Herzegovina, where data were obtained after the year 1995. Between the years 1990 and 1999, bovine tuberculosis was diagnosed in a total of 1 084 cattle herds. Nine hundred and seventy five (89.9%) outbreaks of the disease were reported in small herds (10 cows) and 109 (10.1%) outbreaks in large cattle herds (>10 cows). The last outbreak of bovine tuberculosis in cattle was diagnosed in Slovakia, Slovenia, the Czech Republic, Croatia and Bosnia and Herzegovina in 1993, 1993, 1995, 1999 and 1999, respectively. Bovine tuberculosis was diagnosed under proper quarantine in Slovenia in 37 fattening bulls imported from two European countries before animals were introduced to the targeting farms. Keywords: Mycobacterium bovis, veterinary epidemiology Journal: Veterinární medicína Pages: 45-51 Volume: 47 Issue: 2-3 Year: 2002 DOI: 10.17221/5802-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5802-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200202-0003.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:2-3:id:5802-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Kolář Author-Workplace-Name: Department of Microbiology, Medical Faculty, Palacký University, Olomouc, Czech Republic Author-Name: R. Pantůček Author-Workplace-Name: Department of Microbiology, Medical Faculty, Palacký University, Olomouc, Czech Republic Author-Name: J. Bardoň Author-Workplace-Name: Department of Microbiology, Medical Faculty, Palacký University, Olomouc, Czech Republic Author-Name: I. Vágnerová Author-Workplace-Name: Department of Microbiology, Medical Faculty, Palacký University, Olomouc, Czech Republic Author-Name: H. Typovská Author-Workplace-Name: Department of Microbiology, Medical Faculty, Palacký University, Olomouc, Czech Republic Author-Name: I. Válka Author-Workplace-Name: Department of Microbiology, Medical Faculty, Palacký University, Olomouc, Czech Republic Author-Name: J. Doškář Author-Workplace-Name: Department of Microbiology, Medical Faculty, Palacký University, Olomouc, Czech Republic Title: Occurrence of antibiotic-resistant bacterial strains isolated in poultry Abstract: The main goal of the study was to analyse the occurrence of antibiotic-resistant bacterial strains in poultry in the Czech Republic in 1999-2000. The resistance was determined in 128 selected Escherichia coli, 88 Staphylococcus sp. and 228 Enterococcus sp. strains. The bacterial species were selected to represent gramnegatives and grampositives, the common part of intestinal microflora and also opportunistic pathogens. In Escherichia coli, 97% of strains were found to be resistant to tetracycline, 51% were resistant to ampicillin, and 31% were resistant to piperacillin. Increased frequencies of resistance to ofloxacin and ciprofloxacin (in 10% of the strains) were also found. In staphylococci, increased numbers of strains resistant to erythromycin (39%), clindamycin (19%), tetracycline (14%) and ofloxacin (13%) were observed. In enterococci, 80%, 59% and 34% of the strains were resistant to tetracycline, erythromycin or nitrofurantoin, respectively. A high-level resistance to streptomycin was proved in 22% of the strains. Eleven Enterococcus sp. strains were found to be resistant to vancomycin (vancomycin-resistant enterococci - VRE). Being of the clinical importance, the VRE strains were analysed in detail. Six VRE were identified as Enterococcus faecium VanA, three strains belonged to Enterococcus sp. group III VanB. The remaining two strains were classified as Enterococcus faecium VanB and Enterococcus faecalis VanB, respectively. Based on SmaI macrorestriction analysis, regardless of their resistance type, vancomycin-resistant Enterococcus faecium strains formed a cluster distinct from the control group of vancomycin sensitive strains. Furthermore, within the cluster of vancomycin resistant Enterococcus faecium strains, two clonal lines could be distinguished while the sensitive strains were more heterogeneous. Keywords: poultry, antibiotics, resistance, vancomycin-resistant enterococcus Journal: Veterinární medicína Pages: 52-59 Volume: 47 Issue: 2-3 Year: 2002 DOI: 10.17221/5803-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5803-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200202-0004.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:2-3:id:5803-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Hubálek Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: F. Treml Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: Z. Juicová Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: M. Huady Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: J. Halouzka Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: V. Janík Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: D. Bill Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic Title: Serological survey of the wild boar (Sus scrofa) for tularaemia and brucellosis in South Moravia, Czech Republic Abstract: Sera of 204 wild boars (Sus scrofa), shot by hunters in the South-Moravian district of Beclav during 1993-2001, were tested by microagglutination reaction using safranin-stained antigens of Francisella tularensis and Brucella abortus: 10.8% and 8.7% seroreactors, respectively, were detected. The highest (17%) prevalence of tularaemia antibodies was found in wild boars during 1993-1994 at the beginning of a widespread outbreak of tularaemia in South Moravia that started in 1994, a nonsignificantly lower (13%) seroprevalence in 1995-1996 during the continuing epizootic, whereas it decreased markedly to 3% in the years 1997-2001 during the disappearance of the epizootic. Brucella sp. antibodies were significantly most frequent (15%) in wild boars in the years 1995-1996. This Brucella seroreactivity has been attributed to B. suis biotype 2 (B. melitensis biovar Suis biotype 2 according to new nomenclature) infection, because B. abortus in both cattle and humans (Bang's disease) was eradicated in the former Czechoslovakia in 1964. The hare brucellosis (B. suis biotype 2) has occurred in the Beclav district in a number of natural foci revealing an increased activity since 1994. Keywords: Francisella tularensis, Brucella melitensis biovar Suis, Brucella suis, Sus scrofa, wild boar, wild swine, wildlife, game animals, serosurvey, microagglutination test, complement fixation test, zoonoses, natural foci of diseases Journal: Veterinární medicína Pages: 60-66 Volume: 47 Issue: 2-3 Year: 2002 DOI: 10.17221/5805-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5805-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200202-0005.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:2-3:id:5805-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Šlosárková Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: V. Híbalová Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: I. Literák Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: I. Herzig Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: E. Bártová Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Kodym Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Malý Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Experimental toxoplasmosis in hypoiodemic mice Abstract: The hypothesis, that hypoiodemia of goats causes such a compromise of the immune system, which during subsequent Toxoplasma gondii infections results in clinically more pronounced signs of toxoplasmosis, was verifying in laboratory mouse. Hypoiodemic mice (fed by wheat and supplied by water), normoiodemic mice (fed by wheat and supplied by water containing 1.25 mg KI/l) and the majority of standard mice (fed by commercial grain mixture containing 0.83 mg I/kg) were experimentally infected with T. gondii oocysts or tachyzoites. The susceptibility to acute T. gondii infection was evaluated according to mortality rate. As a criterion of cell-mediated immune function has been chosen the spleen-lymphocyte transformation test (LTT). We observed no difference in LTT between hypoiodemic and normoiodemic mice infected with T. gondii oocysts or tachyzoites and no difference in mortality of both infected groups. Four days after the exposure to 100 tachyzoites of T. gondii (K24 strain), all experimentally infected groups of mice showed statistically significant decrease (P = 0.004) in spleen cells responsiveness to stimulation by all mitogens used - as compared to non-infected standard mice group. Reduced responsiveness of cells was probably caused by T. gondii infection itself - the relation to iodine deficiency has not been found. Keywords: iodine, cell-mediated immune function, Toxoplasma gondii, laboratory mice Journal: Veterinární medicína Pages: 67-74 Volume: 47 Issue: 2-3 Year: 2002 DOI: 10.17221/5806-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5806-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200202-0006.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:2-3:id:5806-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Kostecká Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: J. Blahovec Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Title: Animal insulin-like growth factor binding proteins and their biological functions Abstract: Insulin-like growth factor (IGF-I, IGF-II) action is influenced by until today known eight forms of insulin-like growth factor binding proteins (IGFBPs). They have been obtained not only from some human and animal tissues and body fluids but also from conditioned medium of cell cultures. An important biological property of the IGFBPs is their ability to increase the circulating half-life of the IGFs. They are able to act as potentiators of cell proliferation. As IGFBPs bind to cell surfaces, they may act either to deliver the IGFs to those surfaces for activation of specific receptors or to activate cell responses independently of receptor activation. Phosphorylation, glycosylation and proteolysis of IGFBPs influence their affinity to IGFs. The IGFBPs in the role of inhibitors may block the activity of the IGFs and be used for antimitogenic therapy. In the last time measuring of IGFBPs levels can be used for diagnosis determination of some endocrine diseases or in differential diagnostics. Keywords: insulin-like growth factor, insulin-like growth factor binding protein Journal: Veterinární medicína Pages: 75-84 Volume: 47 Issue: 2-3 Year: 2002 DOI: 10.17221/5807-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5807-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200202-0007.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:2-3:id:5807-VETMED