Template-Type: ReDIF-Article 1.0 Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L. Dvorska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Bartos Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Parmova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Melicharek Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A. Jesenska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Havelkova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Slosarek Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Putova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: G. Martin Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: W. Erler Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Kremer Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: D. Van Soolingen Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Molecular epidemiology of bovine tuberculosis in the Czech Republic and Slovakia in the period 1965-2001 studied by spoligotyping Abstract: Spoligotyping was used to examine IS6110-positive DNA of 26 Mycobacterium bovis, M. bovis BCG and M. bovis subsp. caprae non-viable isolates stored up to 10 years. All of these isolates were previously identified by biochemical tests and all 17/17 tested isolates were earlier found virulent for guinea pigs. In total seven spoligotypes, designated S1-S7, were detected and compared with the spoligotypes of 3 176 isolates in the database of the National Institute of Public Health and the Environment (RIVM) in Bilthoven, the Netherlands. A Neotype M. bovis strain, isolated in 1965 in the USA and thereafter stored in The Czechoslovak National Collection of Type Cultures (My 310/87) since 1987 was of an identical spoligotype S4 with the original reference M. bovis strain from the USA. The M. bovis isolates from capybara's (Hydrochoerus hydrochaeris) imported from Germany to the Czech Republic in 1989, as well as cattle isolates from 1966, 1991 and 1994, were of the most common type S1. Also a human isolate from 1981, a M. bovis BCG vaccine strain and clinical M. bovis BCG isolates from three children with post-vaccinal complications were of this most predominant spoligotype. The four unique spoligotypes S2, S3, S5 and S6 were identified in M. bovis isolates from cattle in the years 1965, 1996 and 1967 in the CzechRepublic, respectively, but also in isolates from farmed red deer (Cervus elaphus) from 1991 and in cattle isolates from Slovakia from the year 1992. The scarcely occurring spoligotype S7, which is typical for M. b. caprae was detected in the Czech Republic from farmed red deer (1999), cattle isolates (1966, 1991, 1995) and in a strain isolated from an 80-year-old man (1999). Several strains isolated in each of three outbreaks in cattle herds were examined. Identical spoligotypes were detected in two outbreaks and different causal agents (M. bovis of spoligotype S1 and M. b. caprae of spoligotype S7) were identified in two cows from the third outbreak. The results confirm an effective control of bovine tuberculosis in the CzechRepublic and Slovakia during 1959-1968, because previously circulating spoligotypes were successfully eradicated. The data also suggest other reservoirs of bovine tuberculosis may exist among free-living wild animals. Keywords: IS6110, Mycobacterium bovis subsp. caprae, Mycobacterium bovis BCG, cattle, capybara, red deer, post-vaccinal complications in children Journal: Veterinární medicína Pages: 181-194 Volume: 47 Issue: 7 Year: 2002 DOI: 10.17221/5822-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5822-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200207-0001.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:7:id:5822-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Trávníček Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: D. Kováčová Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: M.R. Bhide Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: P. Zubrický Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: L. Čisláková Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Title: Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams Abstract: Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%, whereas, in case of rams seroprevalence was 0% by using complement fixation test. Using iELISA in the same groups the seroprevalences observed were, 24.24% in goats, 11.30% in sheep and 5.30% in rams. Indirect ELISA was found to be comparatively more sensitive than CF test in all three groups of animals for detection of IgG antibodies against Chlamydophila abortus. The iELISA used in this study can be used for screening at herd level like CF test, as there is moderate agreement (Kappa - 0.426) between these two tests. Keywords: Chlamydophila abortus, CF test, iELISA, goats, sheep, rams Journal: Veterinární medicína Pages: 195-198 Volume: 47 Issue: 7 Year: 2002 DOI: 10.17221/5823-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5823-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200207-0002.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:7:id:5823-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: GY Huszebucza Author-Workplace-Name: Faculty of Veterinary Science, Szent István University, Budapest, Hungary Author-Name: M. Kulcszr Author-Workplace-Name: Faculty of Veterinary Science, Szent István University, Budapest, Hungary Author-Name: P. Rudas Author-Workplace-Name: Faculty of Veterinary Science, Szent István University, Budapest, Hungary Title: Clinical endocrinology of thyroid gland function in ruminants Abstract: After briefly introducing the basec steps in production and metabolism of thyroid hormones, the author gives an overview of nutritional, metabolic and disease related factors and endocrine interactions influencing thyroid gland function in ruminants, particularly in the postpartum dairy cow. Involvement of thyroid hormonal regulation of seasonal patterns of reproduction as well as ovarian endocrine function are discussed. Keywords: thyroxin, triiodothyronine, ovary, metabolism, ruminants Journal: Veterinární medicína Pages: 199-210 Volume: 47 Issue: 7 Year: 2002 DOI: 10.17221/5824-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5824-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200207-0003.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:7:id:5824-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Pavlik Author-Workplace-Name: Kluwer Academic Publishers, Dordrecht/Boston/London, 2000, 72 pp. ISBN 041 284-1509 Author-Name: J. Kazda Author-Workplace-Name: Kluwer Academic Publishers, Dordrecht/Boston/London, 2000, 72 pp. ISBN 041 284-1509 Title: The Ecology of Mycobacteria Journal: Veterinární medicína Pages: 211-212 Volume: 47 Issue: 7 Year: 2002 DOI: 10.17221/5825-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5825-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:47:y:2002:i:7:id:5825-VETMED