Template-Type: ReDIF-Article 1.0 Author-Name: B. Habrun Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Author-Name: V. Bilič Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Author-Name: Ž. Cvetnič Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Author-Name: A. Humski Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Author-Name: M. Benič Author-Workplace-Name: Croatian Veterinary Institute, Zagreb, Croatia Title: Porcine pleuropneumonia: the first evaluation of field efficacy of a subunit vaccine in Croatia Abstract: A vaccine for porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae was studied in Croatia on a farm infected by agent serotypes 2 and 9. Vaccination with a commercial subunit vaccine was initiated in the second half of 1998 due to the immense economic damage caused on the farm by this disease. All prefattening and fattening pigs kept on the farm during the first three months of 1999 were allocated in two groups: vaccinated and control. In the control and vaccinated group, 226 and 35 animals (5.78% and 0.96% of the average number of prefattening and fattening pigs in control and vaccinated group), respectively, died from pleuropneumonia. The vaccine efficacy was 83.5%. Examination of the randomly selected lungs on the slaughter line revealed significant reduction in the lesions specific for the chronic form of pleuropneumonia in the vaccinated group (vaccine efficacy 78.6%). The tested vaccine significantly decreased the death rate and pulmonary lesions due to A. pleuropneumoniae. Keywords: porcine pleuropneumonia, immunoprophylaxis, vaccine efficacy Journal: Veterinární medicína Pages: 213-217 Volume: 47 Issue: 8 Year: 2002 DOI: 10.17221/5826-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5826-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200208-0001.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:8:id:5826-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Ondrejková Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: J. Suli Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: R. Ondrejka Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: Z. Beníšek Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: R. Franka Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: Š. Švrček Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: M. Maďar Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: A. Bugarský Author-Workplace-Name: Department of Infectious and Parasitic Diseases, University of Veterinary Medicine, Košice, Slovak Republic Title: Comparison of the detection and quantification of rabies antibodies in canine sera Abstract: Rabies antibodies in canine sera were detected and quantified by methods: virus neutralisation test on mice (VNT), rapid fluorescence focus inhibition test (RFFIT), and fluorescent antibody virus neutralisation (FAVN). The results of rabies antibodies levels in non-vaccinated dogs obtained by all three methods were in correlation. The comparison of rabies antibody titres determined in vaccinated dogs using VNT and FAVN methods showed 86.6% correspondence, while those obtained by RFFIT and FAVN methods corresponded in almost 95% of cases. Keywords: rabies antibodies, detection, RFFIT, FAVN test Journal: Veterinární medicína Pages: 218-221 Volume: 47 Issue: 8 Year: 2002 DOI: 10.17221/5827-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5827-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200208-0002.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:8:id:5827-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. R Čož-Rakovac Author-Workplace-Name: Rudjer Boskovic Institute, Center for Marine and Environmental Research, Zagreb, Croatia Author-Name: I. Strunjak-Perović Author-Workplace-Name: Rudjer Boskovic Institute, Center for Marine and Environmental Research, Zagreb, Croatia Author-Name: N. Topić Popović Author-Workplace-Name: Rudjer Boskovic Institute, Center for Marine and Environmental Research, Zagreb, Croatia Author-Name: M. Hacmanjek Author-Workplace-Name: Rudjer Boskovic Institute, Center for Marine and Environmental Research, Zagreb, Croatia Author-Name: B. Šimpraga Author-Workplace-Name: Rudjer Boskovic Institute, Center for Marine and Environmental Research, Zagreb, Croatia Author-Name: E. Teskeredžić Author-Workplace-Name: Rudjer Boskovic Institute, Center for Marine and Environmental Research, Zagreb, Croatia Title: Health status of wild and cultured sea bass in the northern Adriatic Sea Abstract: A complex survey has been conducted in the northern Adriatic Sea over a period of one year that included comparative parasitological, bacteriological, virological, histological and biochemical studies of the cultured and wild sea bass (Dicentrarchus labrax L.). The results show that parasite infestations were due mainly to ectoparasitic monogenea Diplectanum sp. in both cultured and wild sea bass. Philometra sp. and Lernaea sp. were detected in wild sea bass while Triaenophorus sp. and Eimeria sp. were found in reared sea bass. Bacterial pathogens isolated from both reared and wild sea bass belong to Pseudomonadaceae (Pseudomonas sp., P. fluorescens) and unknown Gram-negative bacteria. Moraxellaceae (Acinetobacter sp.), Vibrionaceae (Shewanella putrefaciens), Enterobacteriaceae (Pantoea agglomerans) and Flavobacterium sp. were isolated from reared fish only. Virological examinations were negative. Histological analysis revealed "fatty liver" (fatty infiltration and degeneration) in the cultured fish. Triglyceride, cholesterol and glucose levels were higher in cultured sea bass (2.55 ± 1.77 mmol/l, 3.68 ± 1.43 mmol/l and 9.97 ± 3.33 mmol/l, respectively) than in wild fish (0.80 ± 0.57 mmol/l, 2.95 ± 0.77 mmol/l and 4.79 ± 3.29 mmol/l, respectively). The present paper contributes to establishing a relationship between disease and pathophysiological conditions in wild and cultured fish. Keywords: Adriatic Sea, sea bass, bacteria, parasite, triglyceride, cholesterol, glukose Journal: Veterinární medicína Pages: 222-226 Volume: 47 Issue: 8 Year: 2002 DOI: 10.17221/5828-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5828-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200208-0003.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:8:id:5828-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Cempírková Author-Workplace-Name: University of South Bohemia, Faculty of Agriculture, České Budějovice, Czech Republic Title: Psychrotrophic vs. total bacterial counts in bulk milk samples Abstract: The objective of the study was to determine psychrotrophic bacteria counts (PBC) and total bacterial counts (TBC) in bulk milk samples collected during a longer period (1999 to 2000). Two sets of samples were analysed. Set 1, including samples collected in three herds, was analysed by the Central Laboratory at České Budějovice. TBC was determined using the apparatus Bactoscan, and PBC by culture according to IDF standards. Relative index pi expressing the PBC/TBC ratio was calculated for each sample. The value of pi for Set 1 was 0.09, ranging in herds included in this set from 0.05 to 0.20. The correlation between PBC and TBC was highly significant (r = 0.69; P < 0.01). Correlation coefficients for the individual herds ranged from 0.32 to 0.81. The correlation was highly significant (P < 0.01) in two herds, and significant (P < 0.05) in one herd. Microbiological milk quality is markedly influenced by housing and milking technologies. Another objective of this study was therefore to compare the microbiological quality of milk produced by cows housed in loose boxes and milked in parlours with that produced by cows housed in stanchion barns and milked with pipeline milking machines (Set 2). Bulk milk samples were collected and transported by standard methods and TBC and PBC were determined by culture on GTK agar supplemented with dried inhibitor-free milk according to the IDF standards. The results have confirmed favourable effects of milking in parlours on milk quality expressed in terms of counts of both technologically relevant groups of bacteria. The difference in TBC and PBC between the two milking technologies was significant (P < 0.05). Relative index for the milk obtained in milking parlours was pi= 0.18 and that for the milk from pipeline milking machines pi = 0.23. Correlation coefficients for PBC and TBC were almost identical (r = 0.92; r = 0.93; both P < 0.01). Keywords: milk, total bacterial counts, psychrotrophic bacteria count, relative index, correlation coefficient Journal: Veterinární medicína Pages: 227-233 Volume: 47 Issue: 8 Year: 2002 DOI: 10.17221/5829-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5829-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200208-0004.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:8:id:5829-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Benkirane Author-Workplace-Name: Département de Microbiologie et Maladies Infectieuses, Institut Agronomique et Vétérinaire Hassan II, Rabat, Morocco Author-Name: M.C.L. de Alwis Author-Workplace-Name: Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka Title: Haemorrhagic septicaemia, its significance, prevention and control in Asia Abstract: Haemorrhagic septicaemia (HS) is an endemic disease in most countries of Asia and sub-Saharan Africa. Within the Asian Region, countries can be classified into three categories, on the basis of incidence and distribution of the disease; these are respectively countries where the disease is endemic or sporadic, clinically suspected but not confirmed, or free. Economic losses due to HS are not only confined to losses to the animal industry, but also rice production on account of its high prevalence among draught animals used in rice fields. Only a few attempts have been made to estimate economic losses, the methodologies used in different countries have varied, and many are not based on active surveillance, and a consideration of all components of direct and indirect losses. Most Asian countries rank HS as the most important contagious disease or the most important bacterial disease in cattle and buffaloes. Resource allocation for prevention and control of HS nationally or internationally will evidently depend on a correct estimate of its economic impact. The key factors in prevention and control would be timely and correct reporting, accurate and rapid diagnosis, strategic use of vaccines with the attainment of a high coverage where necessary with a high quality vaccine. National level activities geared towards attainment of these objectives may be with advantage supported and strengthened by international organisations involved in animal health. The present paper attempts to review aspects related to the epidemiology, control and containment of HS in Asia and, proposes some key issues on which a regional programme for HS control in this continent should be centred. Keywords: haemorrhagic septicaemia, prevention, control, vaccine, Asia Journal: Veterinární medicína Pages: 234-240 Volume: 47 Issue: 8 Year: 2002 DOI: 10.17221/5830-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5830-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200208-0005.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:8:id:5830-VETMED