Template-Type: ReDIF-Article 1.0 Author-Name: M. Levkut Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: J. Pistl Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: V. Revajová Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: J. Chroma Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: M. Levkutová Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: V. Dávid Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Title: Comparison of immune parameters in cows with normal and prolonged involution time of uterus Abstract: Indices of cellular immunity in postpartum Holsteincows with the normal (n = 9) and prolonged (n = 4) uterine involution time were evaluated. Peripheral white blood cells were isolated by lysis from postpartum animals. An indirect immunofluorescence method for staining and flow cytometric analysis was employed to determine the cell subpopulation of lymphocytes. The function assays were also used to examine the activity of lymphocytes and phagocytes. A significant decrease in the lymphocyte absolute number, and subpopulation of T (CD2+, CD4+, CD8+), and B (IgM+) cells in dams with postparturient complications and prolonged uterine involution time was observed. The quantitative changes of immune cells were accompanied by a significant decline of phagocyte functional activity in an iodo-nitro-tetrazolium reductase test and polyclonal lymphocyte activation to phytohemagglutinin in a leukocyte migration-inhibition assay. In conclusion, a significant decrease in the lymphocyte absolute number and subpopulation of T (CD2+, CD4+, CD8+), and B (IgM+) cells was observed and the host defense role of phagocytes and lymphocytes was impaired in cows with prolonged uterine involution, which can increase their susceptibility to infections. Keywords: dairy cows, periparturient period, phagocytes, lymphocytes, cell-mediated immune functions, immu-nosuppression, flow cytometry Journal: Veterinární medicína Pages: 277-282 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5835-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5835-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0001.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5835-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Çiftci Author-Workplace-Name: Department of Chemistry, Arts and Science Faculty, Atatürk University, Erzurum, Turkey Author-Name: V. Turkoglu Author-Workplace-Name: Department of Chemistry, Arts and Science Faculty, Atatürk University, Erzurum, Turkey Author-Name: S. Aldemir Author-Workplace-Name: Department of Chemistry, Arts and Science Faculty, Atatürk University, Erzurum, Turkey Title: Effects of some antibiotics on glucose 6-phosphate dehydrogenase in sheep liver Abstract: In vitro effects of penicillin, sulbactam, cefazolin, and amikacine on the activity of the enzyme glucose-6-phosphate dehydrogenase in sheep liver were investigated. Glucose 6-phosphate dehydrogenase was purified from sheep liver, using a simple and rapid method. The purification consisted of two steps, preparation of homogenate and 2', 5'-ADP Sepharose 4B affinity chromatography. As a result of the two consecutive procedures, the enzyme, having the specific activity of 11.76 EU/mg proteins, was purified with a yield of 35.72% and 1.913 fold. In order to control the enzyme purification SDS polyacrylamide gel electrophoresis (SDS-PAGE) was done. SDS-PAGE showed a single band for the enzyme. In addition, I50 values of the antibiotics were determined by plotting activity % vs. antibiotic concentrations. I50 values were 17.71 mM for penicillin, 27.38 mM for sulbactam, 28.88 mM for cefazolin, and 30.59 mM for amikacine. Keywords: glucose 6-phosphate dehydrogenase, antibiotics, sheep, liver Journal: Veterinární medicína Pages: 283-288 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5836-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5836-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0002.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5836-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Trebichavský Author-Workplace-Name: Institute of Microbiology of the Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic Author-Name: H. Kozáková Author-Workplace-Name: Institute of Microbiology of the Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic Author-Name: I. Šplíchal Author-Workplace-Name: Institute of Microbiology of the Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic Title: Plasma lipopolysaccharide level and enterocyte brush border enzymes in gnotobiotic piglets infected with Salmonella typhimurium Abstract: Gnotobiotic piglets were orally infected either with the virulent LT2 strain or the non-pathogenic SF1591 rough mutant of Salmonella enterica serotype Typhimurium. They were sacrificed 6 or 24 h after the infection. All piglets infected for 24 h developed systemic infection with an increase of plasma lipopolysaccharide. Infection with the virulent strain caused a significant decrease (P < 0.001) of gamma-glutamyl transpeptidase (GGT) activity in the enterocyte brush border of both the jejunum and ileum, infection with the rough mutant caused a decrease of GGT activity in the ileum only. The activities of other brush border enzymes (lactase, sucrase, glucoamylase, alkaline phosphatase and dipeptidylpeptidase IV) did not change significantly after infection. Keywords: swine, gnotobiotic piglet, Salmonella typhimurium Journal: Veterinární medicína Pages: 289-294 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5837-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5837-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0003.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5837-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Indik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L. Valíček Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Differentiation of porcine reproductive and respiratory syndrome virus European vaccine strains from Czech field isolates by restriction fragment length polymorphism analysis of ORF5 gene Abstract: Restriction fragment length polymorphism (RFLP) analysis of open reading frame 5 was developed for typing of Czech strains of porcine reproductive and respiratory syndrome virus (PRRSV). The set of restriction enzymes Acc I, Hae II and SnaB I allowed the differentiation of heterogeneous Czech strains of PRRSV clustered separately in the phylogenetic tree. The high-passage strain V-502 (164) was also differentiated from its parent strain V-502. The same restriction enzymes could distinguish the European-type vaccine strains Porcilis PRRS and Pyrsvac-183, registered inCzechRepublic, from the Czech field isolates. The published ORF5 nucleotide sequences allowed us to presume that it will also be possible to distinguish most of European field strains from vaccine strains. PCR-based RFLP analysis can become a valuable tool in epidemiological studies of PRRSV inEurope. Keywords: RFLP, PRRSV, porcilis, pyrsvac, ORF5 Journal: Veterinární medicína Pages: 295-301 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5838-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5838-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0004.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5838-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B. Gradinski-Vrbanac Author-Workplace-Name: Department of Physiology and Radiobiology, Author-Name: Z. Stojevič Author-Workplace-Name: Department of Physiology and Radiobiology, Author-Name: S. Milinkovič-Tur Author-Workplace-Name: Department of Physiology and Radiobiology, Author-Name: T. Balenovič Author-Workplace-Name: Department of Physiology and Radiobiology, Author-Name: J. Piršljin Author-Workplace-Name: Department of Physiology and Radiobiology, Author-Name: M. Zdeler-Tuk Author-Workplace-Name: Department of Physiology and Radiobiology, Title: In vitro susceptibility of duck, chicken, and pig erythrocyte lipids to peroxidation Abstract: The susceptibility of erythrocyte lipid to in vitro peroxidation as measured by TBARS (thiobarbituric acid-reactive substances) and concentration of reduced glutathione (GSH) in whole blood and erythrocyte suspension before and after incubation with hydrogen peroxide was assessed in ducks, chickens and pigs. A high susceptibility of erythrocytes to peroxidation in vitro was observed in all animals tested, but this susceptibility was of different intensity. Pig erythrocytes exhibited the lowest resistance to oxidative stress in vitro as compared with that in ducks (P < 0.01) and chickens (P < 0.02). A high level of GSH in the erythrocytes of ducks and chickens offers higher resistance to oxidative stress in comparison with that in the pig erythrocytes. Keywords: duck, chicken, pig, erythrocytes, thiobarbituric acid-reactive substances, glutathione Journal: Veterinární medicína Pages: 303-308 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5839-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5839-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0005.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5839-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Treml Author-Workplace-Name: Department of Infectious Diseases and Epizootiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Pejčoch Author-Workplace-Name: Department of Infectious Diseases and Epizootiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: Z. Holešovská Author-Workplace-Name: Department of Infectious Diseases and Epizootiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Small mammals - natural reservoir of pathogenic leptospires Abstract: A total of 429 specimens belonging to 9 species of small mammals, trapped in various areas of the CzechRepublicduring the year 2001, were examined for antibodies against leptospires. Antibodies were found in all localities under study in 50 specimens, i.e., 11.6%, belonging to 5 species. Antibodies were most often , i.e., in 42% of positives, found in Microtus arvalis. In Apodemus spp. and Clethrionomys glareolus it was in 34.0% and 14.0% of positives, respectively. In other specimens belonging to Mus musculus and Microtus subterraneus antibodies were found only on occasion. Antibodies against L. grippotyphosa serotype were only found. Titres varied from 100 to 12 800 and most reactions in positive individuals were at lower dilutions, i.e., up to the titre of 800 in 40 cases (80%). The results proved the already known fact of dominant persistence of leptospiral foci of the grippotyphosa type in the Czech Republic. Keywords: free-living mammals, leptospiroses, L. grippotyphosa, serological examinations, antibodies Journal: Veterinární medicína Pages: 309-314 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5840-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5840-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0006.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5840-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Šefčíková Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic Author-Name: Š. Mozeš Author-Workplace-Name: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic Title: Effect of early nutritional experience on the feeding behaviour of adult female rats Abstract: In the present paper the effect of postnatal undernutrition on feeding behaviour has been evaluated in mature female Wistar rats subjected to food restriction a) during the suckling period, i. e. up to day 15 (SR), b) during the weaning period from day 15 to 30 (WR), c) throughout 30 days post partum (SWR) and d) during adulthood, i.e. from day 60-75 of life (AR). In comparison with the ad libitum fed controls (C), the lactating mothers and the pups of the food-restricted groups were allowed free access to food for only two hours daily. In experimental groups this restriction was then changed to an ad libitum regime. On day 90 food intake was evalu-ated daily in five 20 min lasting sessions after 23 h of fasting in group-housed and single-housed rats exposed to their home as well as to their experimental environment. The significantly decreased body weight found on day 30 and 90 in females coming from litters of restrictedly fed mothers, as compared with those fed ad libitum, showed that these animals were considerably undernourished. Appetitive motivation increased in those groups of females that had been exposed to a restricted feeding regime during their infant period but remained unaffected in females restrictedly fed during their adult age. In comparison with the ad libitum fed controls and AR groups, significantly increased mean food intake was observed in all postnatally underfed groups (P < 0.001). The greatest inclination to hyperphagia and qualitative changes in feeding behaviour were observed in SWR females whose feeding regime was restricted for the longest time, i. e. during the suckling and the weaning period, as compared to groups with shorter food restriction. These animals showed hyperphagia even in the new environment regardless whether the whole group or only one female was tested. Our results reveal a) a determinant influence of the feeding regime of the animals at early age (41%) while environmental and social factors represent only about 17% and 4%, respectively and that b) changes in feeding habits in adult female rats may significantly depend on the length of postnatal undernutrition. Keywords: postnatal undernutrition, food intake, environmental and social effects, female rats Journal: Veterinární medicína Pages: 315-322 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5841-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5841-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0007.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5841-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Srebočan Author-Workplace-Name: Department of Pharmacology and Toxicology Author-Name: J. Pompe-Gotal Author-Workplace-Name: Department of Pharmacology and Toxicology Author-Name: I. Kiš Author-Workplace-Name: Clinic for Internal Diseases Author-Name: B. Pirkič Author-Workplace-Name: Clinic for Surgery, Orthopedics and Ophthalmology Author-Name: B. Artukovič Author-Workplace-Name: Department of General Pathology and Patholohical Morphology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Z. Špacir-Prskalo Author-Workplace-Name: Department of Pharmacology and Toxicology Title: Lead intoxication in a puppy - a case report Abstract: Lead intoxication in a puppy is described. The puppy was submitted to surgical procedure for suspicion of acute abdominal pain. Diagnosis was established on the basis of the presence of eroded lead weights in stomach, biochemical and hematological blood tests as well as by elevated tissue lead concentrations. Keywords: lead poisoning, dog Journal: Veterinární medicína Pages: 323-324 Volume: 47 Issue: 10-11 Year: 2002 DOI: 10.17221/5842-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5842-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200210-0008.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:10-11:id:5842-VETMED