Template-Type: ReDIF-Article 1.0 Author-Name: Z. Sládek Author-Workplace-Name: Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: D. Vašíčková Author-Workplace-Name: Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: D. Ryšánek Author-Workplace-Name: Mendel University of Agriculture and Forestry, Brno, Czech Republic Title: The dynamics of morphological changes during in vitro aging of bovine virgin mammary gland neutrophils Abstract: The present study was an in vitro analysis of the dynamics of bovine mammary gland neutrophil apop­tosis based on the detection of morphological changes. The neutrophils were isolated from mammary glands of five virgin heifers. The mammary glands were lavaged, the suspensions were then bacteriologically examined, and total and differential cell counts were made. The cells were cultivated in vitro for 4 hours. After 2, 3 and 4 hours of cultivation, they were panoptically stained, and the proportions of apoptotic neutrophils and trypan blue positive neutrophils were determined. Neutrophil apoptosis and impaired cytoplasmic membrane integrity of neutrophils were already observed in the mammary gland lavages (11.9% and 0.8%, respectively). During the cultivation, a progressive increase in the number of apoptotic neutrophils in various stages of apoptosis - karyopyknosis, zeiosis and apoptotic bodies - was observed. Karyopyknotic neutrophils represented a dominant part of the apoptotic neutrophil population in the course of the whole cultivation. The most intensive increase was observed in zeiosis, whereas the levels of apoptotic bodies remained the same. After 4 hours of cultivation, 31.7% apoptotic neutrophils and 9.8% trypan blue positive neutrophils (i.e. Secondary necrotic cells) were found. The results of this work show that spontaneous apoptosis and secondary neutrophil necrosis must be taken into account during in vitro cultivations of bovine mammary gland neutrophils. Keywords: neutrophil, apoptosis, aging, bovine virgin mammary gland Journal: Veterinární medicína Pages: 325-332 Volume: 47 Issue: 12 Year: 2002 DOI: 10.17221/5843-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5843-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200212-0001.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:12:id:5843-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N. Vijtiuk Author-Workplace-Name: Department of Biology and 3Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: K. Trutin-Ostovič Author-Workplace-Name: Department of Biology and 3Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: T. Balenovič Author-Workplace-Name: Department of Biology and 3Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M. Popovič Author-Workplace-Name: Department of Biology and 3Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: I. Valpotič Author-Workplace-Name: Department of Biology and 3Department of Animal Husbandry, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Functional and phenotypic analyses of porcine gut immune cells immunized by oral administration of F4ac+nonenterotoxigenic Escherichia coli strains Abstract: The aim of this study was to determine the priming effect of experimentally inoculated non-ETEC strains (2407, 1466) on gastrointestinal mucosal lymphocytes. Five 4-week-old pigs per group were orally inoculated with either F4ac+(1466 or 2407) or F4- (1467) non-ETEC strains. The control pigs were given broth containing 1.2% sodium bicarbonate. At postinoculation Day 6 the pigs were killed, their gut lymphocytes were isolated, and their responsiveness was tested in vitro with F4 antigen, peptidoglycan monomer (PGM), pokeweed mitogen (PWM), phytohemagglutinin (PHA) and lipopolysaccharide (LPS). Additionally, the patterns of cluster of differentiation (CD) antigen expression on T and B cells in the single cell suspensions from JLP, IPP, and MLN were determined by flow cytometry using anti-swine CD-specific monoclonal antibodies. F4ac+ non-ETEC strain 2407 and, to a lesser extent 1466, activated lymphocytes from PP and MLN to respond better to common mitogens (PHA, PWM, LPS), purified fimbrial (F4ac) antigen or immunologic response modifier (PGM). An increase of CD2a+and CD8a+T cells in JLP, and species-specific SWC1+T cells in MLN (P < 0.05) was detected in 2407-treated pigs. In conclusion, inoculation with non-ETEC strain 2407 exhibited stimulatory properties to porcine gut immune cells, and thus, could be used in the vaccination programs to control the postweaning colibacillosis in pigs. Keywords: postweaning colibacillosis, lymphocyte, pigs Journal: Veterinární medicína Pages: 333-341 Volume: 47 Issue: 12 Year: 2002 DOI: 10.17221/5844-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5844-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200212-0002.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:12:id:5844-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Paulíková Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: G. Kováč Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: J. Bíreš Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: Š. Paulík Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: H. Seidel Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Author-Name: O. Nagy Author-Workplace-Name: University of Veterinary Medicine, Košice, Slovak Republic Title: Iodine toxicity in ruminants Abstract: The inadequate intake of iodine is still a topical problem, because it contributes to a reduction in performance through the disturbed health of adult animals and their offspring. The necessity of iodine is frequently described in association with hypothyroidal state. In our work we want to assign the hidden risk of prolonged iodine feeding in ruminants. We discuss the possible sources of iodine intoxication, the clinical signs of the intoxication, its effects on production, reproduction, and thyroid functions; animal susceptibility to intoxication; laboratory and necropsy findings; diagnostic, and therapeutic possibilities. Keywords: iodine, intoxication, cattle, sheep, goats Journal: Veterinární medicína Pages: 343-350 Volume: 47 Issue: 12 Year: 2002 DOI: 10.17221/5845-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5845-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200212-0003.txt Handle: RePEc:caa:jnlvet:v:47:y:2002:i:12:id:5845-VETMED