Template-Type: ReDIF-Article 1.0 Author-Name: I. Pavlik Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: W. Yayo Ayele Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: I. Parmova Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: I. Melicharek Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: M. Hanzlikova Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: M. Svejnochova Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: B. Körmendy Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: G. Nagy Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: Z. Cvetnic Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: V. Katalinic-Jankovic Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: M. Ocepek Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: M. Zolnir-Dovc Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: M. Lipiec Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Author-Name: M. Havelkova Author-Workplace-Name: , W. Y A 1, I. P 2, I. M 3, M. H 3, M. S Title: Mycobacterium tuberculosis in animal and human populations in six Central European countries during 1990-1999 Abstract: Results of Mycobacterium tuberculosis detection in animals from six Central European countries (Croatia, the Czech Republic, Hungary, Poland, Slovakia and Slovenia) spreading over 610 402 km2 with a population of 11.8 million heads of cattle were analysed. In the monitoring period, 1990 to 1999, M. tuberculosis from animals was isolated only in two countries (Poland and Slovak Republic) from 16 animals with tuberculous lesions. These comprise nine heads of cattle (Bos taurus), four domestic pigs (Sus scrofa f. domestica) and three wild animals, an African elephant (Loxodonta africana), agouti (Dasyprocta aguti) and terrestrial tapir (Tapirus terrestris), originated form a zoological garden Gdansk in Poland. Steady decrease in the incidence of tuberculosis in humans was recorded during the monitoring period in all countries. The human population of the study countries was 68.03 million. In the period monitored, infection caused by M. tuberculosis was identified in a total of 241 040 patients with a decreasing incidence of tuberculosis found in all countries. The lowest relative bacteriologically confirmed disease was found in theCzechRepublic,SlovakRepublic andSlovenia. Given the low number of infected domestic and wild animals, the epidemiological and epizootiological situation may be considered auspicious. Keywords: Mycobacterium bovis, human tuberculosis, risk assessment Journal: Veterinární medicína Pages: 83-89 Volume: 48 Issue: 4 Year: 2003 DOI: 10.17221/5754-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5754-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200304-0001.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:4:id:5754-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Pavlik Author-Workplace-Name: , W. Y A 1, M. H 2, M. S 3, V. K -J 4, M. Z -D 5 1Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: W. Yayo Ayele Author-Workplace-Name: , W. Y A 1, M. H 2, M. S 3, V. K -J 4, M. Z -D 5 1Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: M. Havelkova Author-Workplace-Name: , W. Y A 1, M. H 2, M. S 3, V. K -J 4, M. Z -D 5 1Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: M. Svejnochova Author-Workplace-Name: , W. Y A 1, M. H 2, M. S 3, V. K -J 4, M. Z -D 5 1Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: V. Katalinic-Jankovic Author-Workplace-Name: , W. Y A 1, M. H 2, M. S 3, V. K -J 4, M. Z -D 5 1Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Author-Name: M. Zolnir-Dovc Author-Workplace-Name: , W. Y A 1, M. H 2, M. S 3, V. K -J 4, M. Z -D 5 1Veterinary Research Institute, Brno, Czech Republic, e-mail: pavlik@vri.cz Title: Mycobacterium bovis in human population in four Central European countries during 1990-1999 Abstract: A survey on Mycobacterium bovis and M. tuberculosis in humans has been performed in four Central European countries (Croatia, the Czech Republic, Slovak Republic and Slovenia) during the years 1990 to 1999. These countries cover an area of 204 688 km2 with 22 135 million population. During the period, new cases of tuberculosis were bacteriologically diagnosed in 47 516 patients. M. tuberculosis was detected in 47 461 (99.88%) cases, whereas M. bovis was found only in 55 (0.12%) patients. The rate of infection due to M. bovis in humans did not exceed 0.29% in the study countries. The annual incidence of bacteriological confirmed M. bovis cases did not exceed 0.1 per 100 000 inhabitants. In the Czech Republic out of 44 tuberculosis patients due to M. bovis, 32 (72.7%) were older than 61 years and originated from rural areas, where they lived during childhood and worked in agricultural occupations. These patients may have suffered a reactivation of persistent (long-standing) M. bovis infection as they got older. Bovine tuberculosis in cattle was eliminated from these countries during the second half of the 1960s (Croatia in 1966, Czech Republic and Slovak Republic - former Czechoslovakia in 1968, Slovenia in 1973) and the incidence of outbreaks of bovine tuberculosis in cattle were very low, thus the disease in humans was unexpected. Keywords: human tuberculosis, animal tuberculosis, risk assessment Journal: Veterinární medicína Pages: 90-98 Volume: 48 Issue: 4 Year: 2003 DOI: 10.17221/5755-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5755-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200304-0002.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:4:id:5755-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Podlasz Author-Workplace-Name: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo II, Poland Author-Name: K. Wąsowicz Author-Workplace-Name: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo II, Poland Author-Name: J. Kaleczyc Author-Workplace-Name: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo II, Poland Author-Name: M. Łakomy Author-Workplace-Name: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo II, Poland Author-Name: R. Bukowski Author-Workplace-Name: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo II, Poland Title: Localization of immunoreactivities for neuropeptides and neurotransmitter-synthesizing enzymes in the pterygopalatine ganglion of the pig Abstract: Study on the presence of the selected biologically active substances in nerve structures of the porcine pterygopalatine ganglion was performed with the use of immunofluorescence and RT-PCR. All neurons in the ganglion were ChAT-, VAChT-, NOS- and VIP- positive. However, some neurons displayed strong immunoreactivity, while in other neurons, immunoreactivity was moderate, or weak. Somatostatin (SOM) was present in approx. 11% of neurons. Tyrosine hydroxylase-positive (TH-positive) neurons were not detected, although in single nerve cell bodies, TH antibody revealed very weak staining which could be attributed to some residual TH immunoreactivity. Immunoreactivity to NPY was found in 25% of all neuronal perikarya while PACAP was present only in 2-3% of them. More numerous neurons (6%) contained immunoreactivity to GAL. No neurons stained for SP or CGRP. Numerous ChAT-, VAChT-, NOS-, VIP-, and PACAP-positive, scarce SP and CGRP-positive, single SOM-, NPY- and GAL-positive nerve fibers were observed throughout the ganglion. No TH immunoreactivity was found in the nerve fibres. RT-PCR detected strong signal of the transcripts of ChAT, SOM, NOS, VIP, NPY, PACAP, and GAL. Only very weak signal was observed in case of TH, SP and CGRP. No RT-PCR was performed for VAChT message. Keywords: pterygopalatine ganglion, pig, immunohistochemistry, RT-PCR Journal: Veterinární medicína Pages: 99-107 Volume: 48 Issue: 4 Year: 2003 DOI: 10.17221/5756-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5756-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200304-0003.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:4:id:5756-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Tomanová Author-Workplace-Name: , J. K 3, J. S 1, R. H 2 1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 2Small Animal Clinic, Faculty of Veterinary Medicine, 3Institute of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: J. Klimeš Author-Workplace-Name: , J. K 3, J. S 1, R. H 2 1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 2Small Animal Clinic, Faculty of Veterinary Medicine, 3Institute of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: J. Smola Author-Workplace-Name: , J. K 3, J. S 1, R. H 2 1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 2Small Animal Clinic, Faculty of Veterinary Medicine, 3Institute of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Husník Author-Workplace-Name: , J. K 3, J. S 1, R. H 2 1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 2Small Animal Clinic, Faculty of Veterinary Medicine, 3Institute of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology Abstract: A nested polymerase chain reaction (PCR) assay and serological examinations were used to detect the presence of Lawsonia intracellularis in a two and a half years old German smooth-coated cocker spaniel with clinical symptoms of chronic diarrhoea and histologically proven inflammatory bowel disease. Fourteen rectal swabs taken over a period of two weeks and eight biopsy specimens taken over a period of six months were used for laboratory examinations. Using the nested PCR, the DNA of L. intracellularis was found in a total of 2 cases, i.e. one rectal swab and one biopsy specimen of the duodenum six months later. The species specificity of the nested PCR product was confirmed by sequencing. The presence of specific IgG antibodies against L. intracellularis was demonstrated by the IFAT in five samples of blood serum taken over a period of seven months. Keywords: Lawsonia intracellularis, nested PCR, indirect immunofluorescence, dog, chronic diarrhoea Journal: Veterinární medicína Pages: 108-112 Volume: 48 Issue: 4 Year: 2003 DOI: 10.17221/5757-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5757-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200304-0004.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:4:id:5757-VETMED