Template-Type: ReDIF-Article 1.0 Author-Name: I. Pavlik Author-Workplace-Name: District Veterinary Administration, Znojmo, Czech Republic Author-Name: L. Matlova Author-Workplace-Name: District Veterinary Administration, Znojmo, Czech Republic Author-Name: L. Dvorska Author-Workplace-Name: District Veterinary Administration, Znojmo, Czech Republic Author-Name: J. Bartl Author-Workplace-Name: District Veterinary Administration, Znojmo, Czech Republic Author-Name: L. Oktabcova Author-Workplace-Name: District Veterinary Administration, Znojmo, Czech Republic Author-Name: J. Docekal Author-Workplace-Name: District Veterinary Administration, Znojmo, Czech Republic Author-Name: I. Parmova Author-Workplace-Name: District Veterinary Administration, Znojmo, Czech Republic Title: Tuberculous lesions in pigs in the Czech Republic in the years 1990-1999: occurrence, causal factors and economic losses Abstract: In the decade monitored a total of 45 873 318 pigs were slaughtered and examined according to veterinary hygiene standards. Apart from 1991, when results of tuberculous findings were not obtained, tuberculous lesions were found in 134 088 (0.32%) of the 41 458 565 pigs examined in the remaining nine years. During a detailed analysis of the pathological anatomical examination of 190 940 pigs slaughtered in one district, tuberculous lesions in lymph nodes were found in 4 107 (2.2%) pigs: mesenteric (65.3% pigs), submandibular (18.6% pigs), inguinal (0.1% pigs) and simultaneously intestinal and head lymph nodes (15.9% pigs). Miliary tuberculosis was found only in the parenchymatous organs of four (0.1%) pigs. The following financial losses resulted: 6% for confiscating the head, intestines and stomach, and from 22 to 24% for assessing meat as conditionally edible after processing, i.e. intended only for heat-processed products. Mycobacteria were isolated from 7 246 (41.8%) pigs through the cultivation of tissue samples from 17 326 pigs. Mycobacterium bovis was detected in only five (0.07%) animals which originated from the last outbreak of bovine tuberculosis in cattle in the Czech Republic in 1995. M. avium complex (MAC) isolates came from 6 870 (94.8%) animals: 55.7% M. a. avium isolates were mainly of serotypes 2 and 3 and genotype IS901+ and IS1245+ and 39.2% M. a. hominissuis isolates were mainly of serotypes 4, 8 and 9 and genotype IS901- and IS1245+. Conditionally pathogenic mycobacteria (M. chelonae, M. terrae, M. phlei and M. fortuitum) were isolated from 371 (5.1%) pigs. In the whole period monitored, two marked increases in the findings of tuberculous lesions were recorded: In the mid-1990s as a result of using deep bedding with wood shavings and at the end of the 1990s as a result of supplementing the pigs' feed with peat. The predominant occurrence of M. a. avium isolates of genotype IS901+ and IS1245+ in the first half of the 1990s was replaced above all by M. a. hominissuis isolates of genotype IS901- and IS1245+. The reason for this was probably a change in the sources of infection for pigs. While at the beginning of the 1990s the most frequent source of infection were wild and domestic birds, various parts of the external environment became the source of the infection for pigs from the mid-1990s. In the years 1996 to 1999, Rhodococcus equi was isolated from 203 (11.6%) of the 1 745 animals examined. It was solely isolated from 154 (8.8%) animals and from 49 (2.8%) animals together with mycobacteria. Keywords: mycobacteriosis, PCR, risk assessment Journal: Veterinární medicína Pages: 113-125 Volume: 48 Issue: 5 Year: 2003 DOI: 10.17221/5758-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5758-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200305-0001.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:5:id:5758-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Gulmez Author-Workplace-Name: Food Hygiene and Technology Department, Faculty of Veterinary, University of Kafkas, Kars, Turkey Author-Name: A. Guven Author-Workplace-Name: Food Hygiene and Technology Department, Faculty of Veterinary, University of Kafkas, Kars, Turkey Title: Survival of Escherichia coli O157:H7, Listeria monocytogenes 4b and Yersinia enterocolitica O3 in ayran and modified kefir as pre- and postfermentation contaminant Abstract: The survival of Escherichia coli O157:H7, Listeria monocytogenes 4b and Yersinia enterocolitica O3 in traditional yogurt and kefir during fermentation, in ayran (a dairy beverage in Turkey), pasteurised (long-life) ayran, modified kefir (salted and diluted kefir) and pasteurised modified kefir during cold storage were investigated. Pasteurised samples were used to monitor the antibacterial effect of natural flora of yogurt and kefir during cold storage. Populations of all the strains were increased during fermentation, and thus pre-fermentation contamination appeared more rhisky than postfermentation contamination. Pasteurisation appeared not to be disaadventageous an application on the microbiological safety of the samples, neverthelessbiological benefits which may come from live microorganisms is lost. While E. coli O157:H7 and L. monocytogenes 4b survived for up to 21 days in all samples, Y. enterocolitica O3 survived only for 14 days in modified kefir. Yogurt microflora appeared to be more suppressive on the pathogens than that of kefir. Keywords: ayran, modified kefir, E. coli O157:H7, L. monocytogenes 4b, Y. enterocolitica O3 Journal: Veterinární medicína Pages: 126-132 Volume: 48 Issue: 5 Year: 2003 DOI: 10.17221/5759-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5759-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200305-0002.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:5:id:5759-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Özsoy Author-Workplace-Name: 1Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey Author-Name: K. Altunatmaz Author-Workplace-Name: 1Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey Title: Treatment of extremity fractures in dogs using external fixators with closed reduction and limited open approach Abstract: Humerus, tibia and antebrachium fractures determined in 30 dogs of different breed, age, weight and gender were treated using Type I and II external fixators. Meynard and handcuff clamps were used in the external fixators. Limited open approach was applied in 6 of the cases and closed reduction techniques in 24. In cases where closed reduction and stabilisation was done, the patients were seen to use their leg within 3-10 days post-operatively and that walking was reasonably good after 20 days. In cases to which a limited open approach had been applied, use of leg was achieved in a period close to the closed method. Keywords: dog, fracture, external skeletal fixation, closed reduction, limited open approach Journal: Veterinární medicína Pages: 133-140 Volume: 48 Issue: 5 Year: 2003 DOI: 10.17221/5760-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5760-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200305-0003.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:5:id:5760-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Husník Author-Workplace-Name: , J. K 5, K. T 2, J. S 2, R. H 3, F. T 4 , J. B 6 1Small Animal Clinic, Faculty of Veterinary Medicine, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 3Department of Pathologic Morphology, Faculty of Veterinary Medicine, 4Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 5Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic Author-Name: J. Klimeš Author-Workplace-Name: , J. K 5, K. T 2, J. S 2, R. H 3, F. T 4 , J. B 6 1Small Animal Clinic, Faculty of Veterinary Medicine, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 3Department of Pathologic Morphology, Faculty of Veterinary Medicine, 4Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 5Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic Author-Name: K. Tomanová Author-Workplace-Name: , J. K 5, K. T 2, J. S 2, R. H 3, F. T 4 , J. B 6 1Small Animal Clinic, Faculty of Veterinary Medicine, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 3Department of Pathologic Morphology, Faculty of Veterinary Medicine, 4Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 5Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic Author-Name: J. Smola Author-Workplace-Name: , J. K 5, K. T 2, J. S 2, R. H 3, F. T 4 , J. B 6 1Small Animal Clinic, Faculty of Veterinary Medicine, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 3Department of Pathologic Morphology, Faculty of Veterinary Medicine, 4Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 5Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic Author-Name: R. Halouzka Author-Workplace-Name: , J. K 5, K. T 2, J. S 2, R. H 3, F. T 4 , J. B 6 1Small Animal Clinic, Faculty of Veterinary Medicine, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 3Department of Pathologic Morphology, Faculty of Veterinary Medicine, 4Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 5Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic Author-Name: F. Tichý Author-Workplace-Name: , J. K 5, K. T 2, J. S 2, R. H 3, F. T 4 , J. B 6 1Small Animal Clinic, Faculty of Veterinary Medicine, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 3Department of Pathologic Morphology, Faculty of Veterinary Medicine, 4Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 5Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic Author-Name: J. Brázdil Author-Workplace-Name: , J. K 5, K. T 2, J. S 2, R. H 3, F. T 4 , J. B 6 1Small Animal Clinic, Faculty of Veterinary Medicine, 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, 3Department of Pathologic Morphology, Faculty of Veterinary Medicine, 4Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, 5Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic 6Institute of Pathologic Anatomy, Faculty Hospital Bohunice, Brno, Czech Republic Title: Lawsonia intracellularis in a dog with inflammatory bowel disease Abstract: A two-year-old male German short-haired pointer was presented with a 1.5-year history of intermittent small-bowel diarrhoea. Inflammatory bowel disease (chronic lymphocytic-plasmacytic gastritis, enteritis and colitis) was diagnosed on the basis of histological examination of biopsies obtained on repeated endoscopy and by exclusion of other possible causes. Warthin-Starry silver staining of stomach mucosa revealed the presence of gastric spiral organisms. The evidence of L. intracellularis was provided by a positive nested polymerase chain reaction in one biopsy of duodenal mucosa and in one rectal smear. In 5 blood sera collected over a period of 8 months the IgG antibodies to L. intracellularis were found by an indirect fluorescent antibody test. Treatment with oral prednisone led only to a temporary improvement. Keywords: Lawsonia intracellularis, dog, diarrhoea, chronic gastritis, enteritis, colitis, inflammatory bowel disease, endoscopy, nested polymerase chain reaction Journal: Veterinární medicína Pages: 141-145 Volume: 48 Issue: 5 Year: 2003 DOI: 10.17221/5761-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5761-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200305-0004.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:5:id:5761-VETMED