Template-Type: ReDIF-Article 1.0 Author-Name: M. Toman Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Faldyna Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Immunological characteristics of cattle with Mycobacterium avium subsp. paratuberculosis infection Abstract: Several years lasting clinical and microbiological monitoring of a cattle herd infected with paratuberculosis allowed to identify three groups of animals showing different courses of the infection. Group N (non-shedders) included animals negative by faecal culture throughout the monitoring period. Animals of Group L (low shedders) shed sporadically small quantities of mycobacteria (1 to 9 CFU), but remained clinically healthy throughout the monitoring period. Group H (high shedders) included animals shedding repeatedly large quantities of Mycobacterium avium subsp. paratuberculosis (10 CFU) with a progressive deterioration of the state of health in most of them. Animals with specific antibodies detected by agar gel immunodiffusion and complement fixation test were found in all groups, but the percentage of serologically positive animals was higher in animals of Group L and significantly higher (p < 0.01) in animals of Group H than in animals of Group N. Specific cell-mediated immunity was demonstrated especially in the group of low shedders (Group L), the differences in the percentages of interferon gamma assay positive animals in this group and other groups was highly significant (p < 0.01). Only insignificant differences in the counts and activity of peripheral leukocytes were found among groups of clinically healthy animals differing in intensity of M. paratuberculosis shedding. However, the progressive development of clinical signs of paratuberculosis in Group H was associated with a significant (p < 0.01) decrease of lymphocytes and monocytes, a non-significant decrease of neutrophils, a significant (p < 0.05) increase in the percentage of CD4+ cells and a insignificant decrease in the percentage of CD8+ cells; consequently the CD4/CD8 ratio increased. Keywords: Johne's disease, cattle, resistance, cell-mediated immunity, interferon-gamma Journal: Veterinární medicína Pages: 147-154 Volume: 48 Issue: 6 Year: 2003 DOI: 10.17221/5762-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5762-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200306-0001.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:6:id:5762-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Herzig Author-Workplace-Name: , J. P 2, B. P 1, E. G 1 1Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Poul Author-Workplace-Name: , J. P 2, B. P 1, E. G 1 1Veterinary Research Institute, Brno, Czech Republic Author-Name: B. Písaíková Author-Workplace-Name: , J. P 2, B. P 1, E. G 1 1Veterinary Research Institute, Brno, Czech Republic Author-Name: E. Göpfert Author-Workplace-Name: , J. P 2, B. P 1, E. G 1 1Veterinary Research Institute, Brno, Czech Republic Title: Milk iodine concentration in cows treated orally or intramuscularly with a single dose of iodinated fatty acid esters Abstract: The effect of a single oral dose of iodinated fatty acid esters (IFAE) on iodine levels in colostrum and milk of goats was tested. In experimental goats that received a single oral dose of IFAE before delivery, significantly higher iodine levels in milk were recorded 60 days after the delivery. In the following period since day 75 after the delivery iodine levels decreased, however, remained higher compared to the control, e.g. on day 152 the levels were twice as high as in the controls. Based on these results, the effect of a single oral and parenteral application of IFAE was tested on dairy cows. The results of the experiment showed that a single oral application of IFAE increases milk iodine levels for a shorter period. Intramuscular application resulted in a significantly higher milk iodine levels during the whole experimental period compared to both untreated controls and cows with oral application of IFAE. Keywords: goat, dairy cows, Lipiodol UF, lactation, iodine in milk, performance Journal: Veterinární medicína Pages: 155-162 Volume: 48 Issue: 6 Year: 2003 DOI: 10.17221/5763-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5763-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200306-0002.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:6:id:5763-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Sakulwira Author-Workplace-Name: , P. V 2, A. T 2, K. O 3, Y. P Author-Name: P. Vanapongtipagorn Author-Workplace-Name: , P. V 2, A. T 2, K. O 3, Y. P Author-Name: A. Theamboonlers Author-Workplace-Name: , P. V 2, A. T 2, K. O 3, Y. P Author-Name: K. Oraveerakul Author-Workplace-Name: , P. V 2, A. T 2, K. O 3, Y. P Author-Name: Y. Poovorawan Author-Workplace-Name: , P. V 2, A. T 2, K. O 3, Y. P Title: Prevalence of canine coronavirus and parvovirus infections in dogs with gastroenteritis in Thailand Abstract: Canine coronavirus (CCV) and canine parvovirus type 2 (CPV-2) are the causative agents of gastroenteritis in dogs. Seventy fecal samples from dogs with signs of gastroenteritis (vomiting and diarrhea), twenty-five fecal samples from healthy dogs and one CPV-2 vaccine strain were amplified by semi-nested polymerase chain reaction (PCR) and semi-nested reverse transcriptase polymerase chain reaction (RT-PCR), aimed at specifically studying the gene encoding the most abundant capsid protein VP2 of CPV-2 and spike protein of CCV. The specificity of the CCV RT-PCR product was evaluated by sequencing. Positive specimens comprised 44 samples (62.8%) and 9 samples (12.8%) for CPV-2 and CCV, respectively. In nine CCV positive samples, seven displayed co-infection between CCV and CPV-2. Our CCV sequence (AF482001) showed a 94.9% nucleotide identity to CCV reported in GenBank accession number D13096. High prevalence of CCV and CPV-2 infections was found in 1-2 month- and 3-6 month-old dogs, respectively. Molecular biology of these viruses is important primarily for epidemic control and preventive measures. Keywords: canine coronavirus, canine parvovirus type 2, gastroenteritis, PCR Journal: Veterinární medicína Pages: 163-168 Volume: 48 Issue: 6 Year: 2003 DOI: 10.17221/5764-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5764-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200306-0003.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:6:id:5764-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Literák Author-Workplace-Name: , B. Š 2, L. V 2 1Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: B. Šmíd Author-Workplace-Name: , B. Š 2, L. V 2 1Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Valíček Author-Workplace-Name: , B. Š 2, L. V 2 1Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Papillomatosis in chaffinches (Fringilla coelebs) in the Czech Republic and Germany Abstract: Analyses of ornithological field records, direct observations of typical lesions, and examination by electron microscopy revealed leg papillomatosis in six chaffinches (Fringilla coelebs) in theCzech Republic and one chaffinch inGermany. Papillomavirus was identified by electron microscopy using the negative staining technique in skin lesions of one chaffinch caught in theCzechRepublic. This is the first report of papillomatosis in chaffinches recorded in theCzechRepublic. Keywords: papilloma, virus, bird, cutaneous lesions, disease Journal: Veterinární medicína Pages: 169-174 Volume: 48 Issue: 6 Year: 2003 DOI: 10.17221/5765-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5765-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200306-0004.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:6:id:5765-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Srebo Author-Workplace-Name: , G. H 3, Ž. G 2, J. P -G 1, R. S Author-Name: G. Hrlec Author-Workplace-Name: , G. H 3, Ž. G 2, J. P -G 1, R. S Author-Name: Ž. Grabarevi Author-Workplace-Name: , G. H 3, Ž. G 2, J. P -G 1, R. S Author-Name: J. Pompe-Gotal Author-Workplace-Name: , G. H 3, Ž. G 2, J. P -G 1, R. S Author-Name: R. Sabo Author-Workplace-Name: , G. H 3, Ž. G 2, J. P -G 1, R. S Title: Poisoning with acetylcholinesterase inhibitors in dogs: two case reports Abstract: Poisoning with acetylcholinesterase inhibitors in dogs is described. Diagnosis is confirmed by presence of carbofuran, chlorpyrifos and their metabolites in livers of poisoned dogs Keywords: acetylcholinesterase inhibitors (carbofuran, chlorpyrifos), dog Journal: Veterinární medicína Pages: 175-176 Volume: 48 Issue: 6 Year: 2003 DOI: 10.17221/5766-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5766-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200306-0005.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:6:id:5766-VETMED