Template-Type: ReDIF-Article 1.0 Author-Name: J. Brichta Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: H. Vesela Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Franek Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Production of scFv recombinant fragments against 2,4-dichlorophenoxyacetic acid hapten using naďve phage library Abstract: Three single chain variable fragment (scFv) antibodies against 2,4-dichlophenoxyacetic acid (2,4-D) herbicide were produced by the Griffin1.library. The selection of the scFv from the phage library was carried out by 2,4-D-protein coated tubes with different levels of hapten substitution in the conjugate. The scFv phage clones were isolated within the five round library panning and the antibodies were expressed in Escherichia coli HB2151. The recombinant products were purified by metal affinity chromatography yielding 200 g of pure scFv per 1 liter of bacterial culture. The antibody fragments provided steep curves in conventional indirect ELISA having the IC50 values from 10.2 to 14.5 ng/ml established for 2,4-D standard. Interestingly enough, the recombinant ScFv E1 antibody exhibited 68% cross-reactivity with 2,4-dichlorphenol (2,4-D = 100%), and 38.0% with methylchlorophenoxyacetic acid (MCPA) whereas reaction with other phenoxyacetic compounds was low. Similar characteristics were obtained for other two recombinant products. Low stability for the isolated scFv antibodies was found in storage buffer even in the presence of stabilizers and protease inhibitors. Factors influencing stability of the recombinant antibodies are discussed. Keywords: naďve phage library, selection, phage antibody, 2, 4-D hapten, antibody stability Journal: Veterinární medicína Pages: 237-247 Volume: 48 Issue: 9 Year: 2003 DOI: 10.17221/5776-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5776-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200309-0001.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:9:id:5776-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Y. Cevger Author-Workplace-Name: Department of Livestock Economics, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: S. Sariozkan Author-Workplace-Name: Department of Livestock Economics, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: H. Guler Author-Workplace-Name: Department of Livestock Economics, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Title: impact of manual and mechanical cut-up of broiler carcasses on the enterprise income Abstract: This research was carried out in the Meat and Fish Products Co. Sincan Plant (Ankara,Turkey) between March 2002-May 2002. Total 666 broiler carcasses, 461 of which was cut-up with hand using a knife and 205 of which was cut up with cutting saw, formed the research material. Cut-up carcasses were classified in 6 groups according to their weights. The carcasses were weighted as a whole in both methods and after their weights were recorded, they were cut-up into 4 parts as breast, legs, wings and neck. It was determined that process of cutting up with knife lasted for 60 s and for 17 s with cutting saw. As a result of cutting up, net income increase was determined as 13.82 percent in cutting up with knife and as 15.61 in cutting up with cutting saw with summer prices and as 3.73 percent in cutting up with knife and as 5.6 percent in cutting up with cutting saw with winter prices. Keywords: optimum cut-up weight, marketing style, seasonal differences, shrinkage Journal: Veterinární medicína Pages: 248-253 Volume: 48 Issue: 9 Year: 2003 DOI: 10.17221/5777-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5777-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200309-0002.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:9:id:5777-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Valentova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein Abstract: Bovine respiratory syncytial virus (BRSV) and related human respiratory syncytial virus (HRSV) are major respiratory tract pathogens in calves and infants, respectively. Great attention is now paid to prevention of the disease caused by these agents. Glycoprotein G is the most variable viral protein and antigenic grouping of RSV isolates is based on distinct antigenic reactivity patterns determined with a set of G protein specific mAbs. Genetic variability of the G protein is used during epidemiology and epizootiology studies of HRSV and BRSV diseases, respectively. The constant genetic drift can be observed within G protein sequences. Both cell-mediated and antibody-mediated immune responses contribute to efficient protection against RSV infection. The neutralizing antibodies are induced by F and G proteins. The G protein fails to induce cytotoxic lymphocytes response and may causes aberrant Th2 response leading to enhancement of clinical symptoms in subsequently infected vaccines. The G as the most variable viral protein associated with immunopathologic effect is a critical factor in vaccine development. Keywords: bovine respiratory syncytial virus, glycoprotein G, antigenic variability, genetic variability, immune response Journal: Veterinární medicína Pages: 254-266 Volume: 48 Issue: 9 Year: 2003 DOI: 10.17221/5778-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5778-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200309-0003.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:9:id:5778-VETMED