Template-Type: ReDIF-Article 1.0 Author-Name: M. Erat Author-Workplace-Name: Biotechnology Application and Research Center, Author-Name: H. Sakiroglu Author-Workplace-Name: Biotechnology Application and Research Center, Author-Name: M. Ciftci Author-Workplace-Name: Biotechnology Application and Research Center, Title: Effects of some antibiotics on glutathione reductase from bovine erythrocytes Abstract: This paper describes effects of some antibiotics on glutathione reductase in vitro. Glutathione reductase was purified from bovine erythrocytes. Purification procedure consisted of four steps; preparation of hemolysate, ammonium sulphate precipitation, 2',5'-ADP Sepharose 4B affinity chromatography and Sephadex G-200 gel filtra­tion chromatography. As a result of these four consecutive procedures, the enzyme having the specific activity of 62.5 EU/mg proteins was purified 31 250-fold with a yield of 11.39%. The purified enzyme showed a single band on sodium dodecyl sulfate polyacrilamide gel electrophoresis (SDS-PAGE). The effects of eight different antibiot­ics-streptomycin sulphate, gentamicin sulphate, netilmicin, teicoplanine, thiamphenicol, ampicillin, cefotaxime, and cefodizime- were investigated on the purified enzyme. Six of these antibiotics (streptomycin sulphate, gentamicin sulphate, netilmicin, teicoplanine, thiamphenicol, and ampicillin) increased the activity of glutathione reductase with increase in their concentrations while the two (cefotaxime and cefodizime) inhibited the enzyme activity. I50 values were 7.713 mM and 1.954 mM, and Ki constants were 11.011 mM and 8.956 mM for cefotaxime and cefodizime, respectively. Their inhibition types were competitive. Keywords: glutathione reductase, bovine erythrocyte, antibiotic Journal: Veterinární medicína Pages: 305-312 Volume: 48 Issue: 11 Year: 2003 DOI: 10.17221/5784-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5784-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200311-0001.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:11:id:5784-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Koonradova Author-Workplace-Name: , J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: J. Uhlik Author-Workplace-Name: , J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: L. Vajner Author-Workplace-Name: , J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: J. Herget Author-Workplace-Name: , J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic Author-Name: J. Adaskova Author-Workplace-Name: , J. U 1, L. V 1, J. H 2, J. A 3 1Institute of Histology and Embryology, 2Institute of Physiology and Centre for Experimental Cardiovascular Research, 2nd Medical Faculty, Charles University, Prague, Czech Republic Title: Mild hyperoxia induces moderate pathological alteration in airway epithelium (ultrastructural study) Abstract: The ultrastructure of the tracheal epithelium in rabbits exposed for 96 hours to 35-37% O2 was studied in our experiments. Due to the influence of mild normobaric hyperoxia, massive differentiation of new secretory elements was initiated and resulted in apparent changes in goblet cells distribution. 60 ± 4% of goblet cells took part in the formation of voluminous intraepithelial mucous glands. Ciliated cells were less damaged than the goblet ones. Tiny signs of pathological alteration of deeper portions of their cytoplasm and apical blebbing accompanied with destruction of some kinocilia were encountered. The ciliary border was slightly impaired. Mild, but significant decrease in the mean number of kinocilia/μm2 went along with significant increase in percentage of altered cilia. Among the altered kinocilia, the slightly altered pathological cilia with local swellings of the ciliary membranes or with tiny vacuoles situated in their shafts were the most numerous. Hyperoxia did not influence the process of ciliogenesis in the ciliated cells. As morphological signs of impairment of the vital self-cleaning ability of the airway epithelium, layers of inspissated mucus were encountered in the area of the ciliary border. From morphological point of view, mild hyperoxia caused moderate damage to the airway epithelium. Keywords: trachea, oxygen, ultrastructure, rabbit Journal: Veterinární medicína Pages: 313-320 Volume: 48 Issue: 11 Year: 2003 DOI: 10.17221/5785-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5785-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200311-0002.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:11:id:5785-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Cvetnic Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: J. Margaletic Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: J. Toncic Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: N. Tturk Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: Z. Milas Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: S. Spicic Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: M. Lojkic Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: S. Terzic Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: L. Jemersic Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: A. Humski Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: M. Mitak Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: B. Habrun Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Author-Name: B. Krt Author-Workplace-Name: , J. M 2, J. T 3, N. T 4, Z. M 4, S. S 1, M. L 1, S. T 1, L. J 1, A. H 1, M. M 1, B. H 1, B. K 5 1Croatian Veterinary Institute Zagreb, Croatia Title: A serological survey and isolation of leptospires from small rodents and wild boars in the Republic of Croatia Abstract: In total the samples of blood and kidneys of 379 small rodents and 154 wild swine were analysed. The antibodies to different serovars of leptospires were determined in 12.7% of small rodents, most often in the species Mus musculus (34.4%), A. agrestis (14.8%), A. flavicolis (10.8%), C. glareolus (9.4%) and A. sylvaticus (6.5%). Most frequent were the findings of antibodies to sv. pomona (27.1%), sv. sejroe (20.8%), and sv. australis (14.6%), and the antibodies to sv. hardjo, sv. saxkoebing, sv. tarassovi, sv. grippotyphosa, sv. bataviae and sv. icterohaemorrhagiae were also established. Seventeen (4.5%) isolates were identified, belonging to the serogroups sejroe (10 isolates), pomona (4 isolates) and australis (1 isolate) and one isolate was not identified. In wild swine positive reactions were estab­lished in 26% of the blood sera analysed. Most frequently the antibodies to sv. pomona (47.5%), sv. australis (40%), sv. grippotyphosa (10%) and sv. icterohaemorhagiae (2.5%) were established. Thirteen (8.4%) isolates belonging to the serogroups pomona (10 isolates), australis (2 isolates) and icterohaemorhagiae (1 isolate) were identified. Keywords: leptospirosis, small rodents, wild boars, prevalence, Croatia Journal: Veterinární medicína Pages: 321-329 Volume: 48 Issue: 11 Year: 2003 DOI: 10.17221/5786-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5786-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200311-0003.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:11:id:5786-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Schlegelova Author-Workplace-Name: , J. B 2, E. K 2,, E. N 3, V. B 1 1Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Brychta Author-Workplace-Name: , J. B 2, E. K 2,, E. N 3, V. B 1 1Veterinary Research Institute, Brno, Czech Republic Author-Name: E. Klimova Author-Workplace-Name: , J. B 2, E. K 2,, E. N 3, V. B 1 1Veterinary Research Institute, Brno, Czech Republic Author-Name: E. Napravnikova Author-Workplace-Name: , J. B 2, E. K 2,, E. N 3, V. B 1 1Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Babak Author-Workplace-Name: , J. B 2, E. K 2,, E. N 3, V. B 1 1Veterinary Research Institute, Brno, Czech Republic Title: The prevalence of and resistance to antimicrobial agents of Bacillus cereus isolates from foodstuffs Abstract: The study was aimed at the assessment whether foodstuffs contaminated with Bacillus cereus (B. cereus) may concurrently be vectors of spreading resistance. The contamination of foodstuffs with B. cereus strains was found in 31% of dairy and in 28% of meat products tested. Only one product from skimmed milk was contaminated. High-fat milk products that were heat-treated during the technological process (87 samples), as well as heat-treated meat products (65 samples), were contaminated significantly frequently (63% and 48% of the samples respectively) (P < 0.01). Almost all B. cereus isolates displayed low susceptibility to ampicillin, cephalothin, and to oxacillin. Except for streptomycin (STR) resistance, resistance to other 8 antimicrobial agents occurred sporadically. The STR resistant isolates came particularly from spreading buffer (8 samples) (P < 0.05). It was established that the same samples were contaminated with two subpopulations of B. cereus with different STR resistances. The frequent occurrence of B. cereus in foodstuffs with either fat content and/or subject to heat treatment in processing makes these products risky, however, our study did not confirm that foodstuffs contaminated with B. cereus are concurrently vectors of transmissible resistance genes. Keywords: pathogenic microorganisms, food safety, acquired resistance Journal: Veterinární medicína Pages: 331-338 Volume: 48 Issue: 11 Year: 2003 DOI: 10.17221/5787-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5787-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200311-0004.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:11:id:5787-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Mutlu Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey Author-Name: S.E. Acar Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey Author-Name: C. Perk Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Istanbul, Turkey Title: Treatment of a case of tracheal stenosis in a dog with tracheal resection and anastomosis Abstract: A case of tracheal stenosis in the cervical portion of the trachea was encountered in a 5.5-month-old St. Bernard-Ro⣸84;weiler cross dog. Breathing difficulty was seen in the clinical examination and presence of an obvious narrowing between the 3rd-5th cervical tracheal rings was determined in the radiological examination. Under general anesthesia the portion with stenosis was resected and the healthy trachea ends were anastomosed using the split cartilage technique. In the postoperative period the breathing difficulty disappeared and there was no development of a new stenosis in the anastomosis region. In the late period check-up the patient was seen to lead a healthy life. Keywords: tracheal, stenosis, resection, anastomose, dog Journal: Veterinární medicína Pages: 339-342 Volume: 48 Issue: 11 Year: 2003 DOI: 10.17221/5788-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5788-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200311-0005.txt Handle: RePEc:caa:jnlvet:v:48:y:2003:i:11:id:5788-VETMED