Template-Type: ReDIF-Article 1.0 Author-Name: T. Amemori Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L. Matlova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: O. A Fischer Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: W. Y Ayele Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Machackova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: E. Gopfert Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Distribution of Mycobacterium avium subsp. paratuberculosis in the gastrointestinal tract of shedding cows and its application to laparoscopic biopsy Abstract: The gastrointestinal tract (GIT) is a major target for Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) in cattle. Culture examination was achieved in tissue samples obtained from 10 different regions of the GIT (proximal and distal parts of the duodenum, proximal, middle and distal parts of the jejunum, proximal and distal parts of the ileum, the ileocecal valve, the caecum and the rectum) and their adjacent lymph nodes. The culture results were statistically analysed to elucidate the distribution of M. a. paratuberculosis in the GIT. A total of 63 cows older than 24 months were diagnosed with paratuberculosis by faecal and tissue cultures. The better detection rate of M. a. paratuberculosis was found in the mucosae from the jejunum to the ileocecal valve and in the lymph nodes from the jejunum to the caecum. The mean number of colony forming units (CFU) in the mucosae and the lymph nodes of the distal jejunum and the proximal ileum was significantly higher than that in the mucosae of the duodenum, the caecum and the rectum, and in the lymph nodes of the duodenum and the rectum, respectively (p < 0.05). Laparoscopic biopsy attempted out on 4 animals to test its potential use for sample collection from the statistically optimal mesenteric lymph nodes; but resulted in an abortive attempt because these targets were encircled by the intestines, the pressure of which complicated the laparoscopic approach. Keywords: Johne's disease, diagnostic, IS900, PCR Journal: Veterinární medicína Pages: 225-236 Volume: 49 Issue: 7 Year: 2004 DOI: 10.17221/5699-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5699-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200407-0001.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5699-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Kajerova Author-Workplace-Name: , V. B 2, I. L 1 1Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: V. Barus Author-Workplace-Name: , V. B 2, I. L 1 1Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: I. Literak Author-Workplace-Name: , V. B 2, I. L 1 1Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Title: New records of Ascaridia platyceri (Nematoda) in parrots (Psittaciformes) Abstract: The aim of the study was to determine the range of species of ascarids in parrots in the CzechRepublic. Ascarids were found during post-mortem parasitological examination of 38 psittaciform birds belonging to 15 different species. All ascarids found were determined as Ascaridia platyceri. Nine bird species were determined as new hosts of this parasite. A. platyceri is a typical ascarid for parrots of Australian origin. The fact that this parasite was found in bird species of African origin demonstrated a possibility of spread of A. platyceri to hosts of different zoogeographical origin. A. platyceri was described in detail from the host Melopsittacus undulatus and differentiated from other ascarids on the basis of morphological and quantitative traits. The most important differentiating traits included the presence of interlabia in both sexes. In males, the traits important for species identification included the number and location of caudal papillae (a total of 9 to 10 pairs), relatively short spicula and absence of cuticular alae on the spicula, while females featured a conical shape of the tail. Keywords: ascarids, morphology, nematodes, CzechRepublic, birds Journal: Veterinární medicína Pages: 237-242 Volume: 49 Issue: 7 Year: 2004 DOI: 10.17221/5700-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5700-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200407-0002.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5700-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Navratilova Author-Workplace-Name: , J. S 2, A. S 2, E. N 1, J. L 1, E. K Author-Name: J. Schlegelova Author-Workplace-Name: , J. S 2, A. S 2, E. N 1, J. L 1, E. K Author-Name: A. Sustackova Author-Workplace-Name: , J. S 2, A. S 2, E. N 1, J. L 1, E. K Author-Name: E. Napravnikova Author-Workplace-Name: , J. S 2, A. S 2, E. N 1, J. L 1, E. K Author-Name: J. Lukasova Author-Workplace-Name: , J. S 2, A. S 2, E. N 1, J. L 1, E. K Author-Name: E. Klimova Author-Workplace-Name: , J. S 2, A. S 2, E. N 1, J. L 1, E. K Title: Prevalence of Listeria monocytogenes in milk, meat and foodstuff of animal origin and the phenotype of antibiotic resistance of isolated strains Abstract: In 2000-2002 samples of raw materials (milk and beef, 518 samples), ready-to-cook foods and final products from milk (30 and 200 samples) and from meat (105 and 110 samples) and swabs from surfaces in two meat-processing plants (41 samples) were examined for the presence of Listeria monocytogenes (L. monocytogenes). 70 isolates were tested using the standard microdilution method for the susceptibility to 12 antimicrobial drugs, minimum inhibitory concentration (MIC) characteristics (MICm, MIC50, MIC90) were determined. L. monocytogenes was isolated from raw milk samples (15 samples). It was not isolated from any of the semi-finished and final milk products except for one sample of pasteurized milk. Furthermore L. monocytogenes was isolated from samples of raw beef, meat emulsion, fermented dry meat products and from swabs from production equipment. In vitro testing of susceptibility showed the considerable effectiveness of examined antimicrobial substances with streptomycin and norfloxacin being least effective (MICm = 4 μg/ml). Apart from the intermediate resistance (MIC = 1-2 μg/ml) and resistance (MIC = 4 μg/ml) to clindamycin (37 and 5 strains, respectively), all strains were susceptible to the tested substances. While the presence of L. monocytogenes in foodstuffs, in particular, are serious, current tests of susceptibility of L. monocytogenes isolates indicate low probability untreatable infections as a result of resistant strains from foods or transfer of resistance to other microorganisms in the CzechRepublic. Keywords: pathogenic bacteria, acquired resistance, food safety Journal: Veterinární medicína Pages: 243-252 Volume: 49 Issue: 7 Year: 2004 DOI: 10.17221/5701-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5701-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200407-0003.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5701-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. S. Rahman Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, South Korea Title: Polymerase chain reaction assay for the diagnosis of experimentally infected pregnant Sprague-Dawley rats with Brucella abortus biotype 1 Abstract: In order to diagnose the experimentally infected pregnant Sprague-Dawley (SD) rats with Brucella abortus biotype 1 using polymerase chain reaction (PCR) assay, the SD rats were injected subcutaneously at the dose of 1.0 × 109 colony forming units (cfu) at different stages of gestation period. The maximum rectal temperature was recorded as 38°C in the infected group within 3 days, whereas in the control group the temperature remained normal (36°C). There were no stillbirths, abortions or premature birth and relapsing fever in the infected SD rats. The pathological findings of infected SD rats were splenomegaly, metritis, swelling of lymph nodes, placentitis associated with lymphocytic and macrophage infiltration. Four hundred ninety-eight base pair DNA was detected in infected tissues through AMOS (Brucella abortus, Brucella melitensis, Brucella ovis, Brucella suis) PCR assay. The AMOS PCR assay was shown to be a valuable tool for diagnosis of infected pregnant Sprague-Dawley rats with B. abortus biotype 1. Keywords: Brucella abortus biotype 1, Sprague-Dawley rats, polymerase chain reaction, South Korea Journal: Veterinární medicína Pages: 253-258 Volume: 49 Issue: 7 Year: 2004 DOI: 10.17221/5702-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5702-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200407-0004.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5702-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Franka Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: S. Svrcek Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: M. Madar Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: M. Kolesarova Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: A. Ondrejkova Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: R. Ondrejka Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: Z. Benisek Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: J. Suli Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Author-Name: S. Vilcek Author-Workplace-Name: , S. S 1, M. M 1, M. K 2, A. O 3, R. O 3, Z. B Title: Quantification of the effectiveness of laboratory diagnostics of rabies using classical and molecular-genetic methods Abstract: In comparative experiments the diagnostic effectiveness of four methods of laboratory diagnostics of rabies - the mouse intracerebral inoculation test (MICIT, MIT), the rabies tissue culture infection test (RTCIT), the rapid rabies enzyme immune diagnosis test (RREID) and a molecular-genetic method, the nested reverse transcription polymerase chain reaction (nRT-PCR) - was quantified by the titration of serial dilutions of brain viral suspensions. The threshold value of the tests, i.e., the highest dilution of a specimen, which the method used is able to detect as a positive one, was determined. Further advantages and disadvantages of the tested methods were compared as well. Experimental optimization of procedures for RNA extraction was carried out and the optimum primer for RNA transcription to cDNA was selected. The RREID method was carried out in two variants: detection of the rabies antigen in a clarified (centrifugated) as well as in a non-clarified (noncentrifugated) brain suspension. In the experiments three autochthonous street isolates of rabies virus (in the form of primary isolates) were used; they had been isolated from naturally infected red foxes (Vulpes vulpes) and a lynx (Lynx lynx). The results of comparative experiments revealed a relative correlation of the diagnostic effectiveness of standard methods (MICIT and RTCIT), with standard MICIT being the more sensitive one, RTCIT however having several other advantages (among others the speed of performance) and thus being preferred. For quantitative comparison of diagnostic effectiveness two other methods (RREID and nRT-PCR) were examined in that street isolates of rabies virus, which revealed the highest titer after titration by MICIT and RTCIT. The sensitivity of the RREID method proved to be rather low. If used with noncentrifugated brain suspensions this method may yield nonspecific reactions. If compared particularly with RREID the nRT-PCR is characterized by a considerably higher diagnostic effectiveness. The sensitivity of nRT-PCR is not affected by preliminary clarification of the brain suspension. The reverse primer N12 seems to be more suitable for transcription of the extracted RNA to cDNA than random hexamers. Keywords: rabies, rabies virus (RABV), MICIT - mouse intracerebral inoculation test, MIT - mouse inoculation test, RTCIT - rabies tissue culture infection test, RREID - rapid rabies enzyme immune diagnostic test, nRT-PCR - nested reverse transcription polymerase chain reaction Journal: Veterinární medicína Pages: 259-267 Volume: 49 Issue: 7 Year: 2004 DOI: 10.17221/5703-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5703-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200407-0005.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5703-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Yaman Author-Workplace-Name: Department of Pathology, Author-Name: T. Durgun Author-Workplace-Name: Department of Pathology, Author-Name: E. Karabulut Author-Workplace-Name: Department of Pathology, Title: Case report of a myxoma in a gamecock Abstract: A mass was detected on the right side of the crop of a 17-month-old gamecock. Macroscopically, the excised mass, 10 × 7.0 × 4.0 cm in size, was ovoid-shaped with a smooth surface and a few lobules. Microscopically, the individual neoplasm cell was stellate or fusiform in shape and the cell nucleus was round, ovoid or elongated, with multiple nucleoli. No hypercellular or pleomorphic areas were identified and no mitoses were observed. The mass was myxoma according to the macroscopic and microscopic results of this study. Keywords: myxoma, gamecock Journal: Veterinární medicína Pages: 268-270 Volume: 49 Issue: 7 Year: 2004 DOI: 10.17221/5704-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5704-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200407-0006.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:7:id:5704-VETMED