Template-Type: ReDIF-Article 1.0 Author-Name: K. Hruska Author-Workplace-Name: Veterinary Research Institute2, Brno, Czech Republic Title: Research on paratuberculosis: Analysis of publications 1994-2004 Abstract: The Web of Knowledge Results Analysis of papers published on paratuberculosis in 1994-2004 demonstrated the increasing interest in Mycobacterium avium subsp. paratuberculosis (MAP). In the analyzed period 1 032 papers published by 2 519 authors affiliated with 738 institutions were indexed in the Web of Science database. The papers were published in 238 journals, 25 of which contained more than 55% of articles. The Top 50 authors, Top 50 institutions and Top 50 most frequently cited papers are listed in this review. The contribution of OIE Reference Laboratory for Paratuberculosis established in the Veterinary Research Institute, Brno, is assessed according to the number of publications (29), number of authors participating (79), number of institutions collaborating (41 from 17 countries) and their positions in the Top 50 lists. It is evident that the number of papers on Mycobacterium avium subsp. paratuberculosis, a species causing paratuberculosis in ruminants and possibly having a role in the development of Crohn's disease in at least some humans is significantly increasing. Keywords: Web of Knowledge, Web of Science, Crohn's disease, paratuberculosis Journal: Veterinární medicína Pages: 271-282 Volume: 49 Issue: 8 Year: 2004 DOI: 10.17221/5705-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5705-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200408-0001.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:8:id:5705-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. M Bruckmaier Author-Workplace-Name: ,2, C. E. O 1, J. W. B 1 1Division of Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, Vetsuisse Faculty, University of Berne, Berne, Switzerland Author-Name: C. E Ontsouka Author-Workplace-Name: ,2, C. E. O 1, J. W. B 1 1Division of Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, Vetsuisse Faculty, University of Berne, Berne, Switzerland Author-Name: J. W Blum Author-Workplace-Name: ,2, C. E. O 1, J. W. B 1 1Division of Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, Vetsuisse Faculty, University of Berne, Berne, Switzerland Title: Fractionized milk composition in dairy cows with subclinical mastitis Abstract: Mastitis is the inflammatory reaction of the udder to invading pathogens. One of the most apparent reactions is the increased influx of immunoreactive cells from blood into milk inducing a dramatic increase of milk somatic cell counts (SCC). We have investigated (i) the relationship between log SCC/ml in infected quarters being >6 (n = 8, group I) or varying between 5.4 and 6 (n = 8, group II) and concentration of dry matter (DM), fat, protein, lactose, insulin-like growth factor (IGF)-1, insulin, prolactin, tumor necrosis factor (TNF)-, sodium, potassium, chloride, electrical conductivity and osmolarity as compared with the contralateral (healthy) quarter (log SCC/ml <5.2); and (ii) composition of fractionized milk [cisternal milk, quartiles of alveolar milk and residual milk (after i.v. injection of 10 u.i. oxytocin)] during machine milking of infected and healthy quarters. SCC were higher (P < 0.05) in infected than in healthy quarters. Concentrations of fat, sodium, chloride, and IGF-1 were higher (P < 0.05), while that of lactose was lower (P < 0.05) in infected than in healthy quarters (group I). Concentrations of fat and chloride in both groups, of DM (in group II), and electrical conductivity and sodium (in group I) increased from the cisternal to alveolar (100%) fractions in infected quarters, while fat and DM concentrations similarly increased in healthy quarters. In conclusion, several but not all milk traits changed in a different manner during the course of milking in infected and non-infected quarters. Keywords: cows, machine milking, mastitis, somatic cell counts Journal: Veterinární medicína Pages: 283-290 Volume: 49 Issue: 8 Year: 2004 DOI: 10.17221/5706-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5706-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200408-0002.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:8:id:5706-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Piccione Author-Workplace-Name: Department of Morphology, Biochemistry, Physiology and Animal Production, Section of Physiology, Faculty of Veterinary Medicine, University of Messina, Italy Author-Name: A. Assenza Author-Workplace-Name: Department of Morphology, Biochemistry, Physiology and Animal Production, Section of Physiology, Faculty of Veterinary Medicine, University of Messina, Italy Author-Name: F. Fazio Author-Workplace-Name: Department of Morphology, Biochemistry, Physiology and Animal Production, Section of Physiology, Faculty of Veterinary Medicine, University of Messina, Italy Author-Name: M. Percipalle Author-Workplace-Name: Department of Morphology, Biochemistry, Physiology and Animal Production, Section of Physiology, Faculty of Veterinary Medicine, University of Messina, Italy Author-Name: G. Caola Author-Workplace-Name: Department of Morphology, Biochemistry, Physiology and Animal Production, Section of Physiology, Faculty of Veterinary Medicine, University of Messina, Italy Title: Assessment of anaerobic threshold in the galloper using a standardised exercise field test Abstract: In this study an incremental load triangular exercise test has been performed on 20 Gallopers, divided into two groups, A and B, and the test has been customised to the two groups according to age and sex. The subjects being tested, after a 10 minutes warm-up, have undergone an incremental three workloads exercise test. All steps lasted for three minutes and were separated by 1 minute intervals (incremental loads triangular exercise test). The workload for each phase of the test has been set as follows: group A, speed of 400, 500 and 600 m/min over a distance of 1 200, 1 500 and 1 800 m; group B, speed of 500, 600 and 700 m/min over a distance of 1 500, 1 800 and 2 100 m. Each horse had a heart rate monitor for the assessment of the heart rate mean value at each phase. At rest, after warm up, and at the end of each phase, 30 and 45 minutes after the end of the test, all subjects underwent a blood test by means of an external jugular venipuncture for the immediate assessment of lactate on whole blood with a portable blood lactate analyser. For each horse the following physical attitude parameters have been calculated: VLa2, VLa4, V200, HR2, HR4. The highly significant correlation between heart rate and speed during the test has shown a linear increment for group A (r = 0.94; p < 0.01) and for group B (r = 0.87; p < 0.01), while the incremental trend of blood lactate as related to speed is exponentially correlated for group A (r= 0.84; p < 0. 01) and for group B (r = 0.85; p < 0.01). The following differences are of statistical significance: V200 of group A compared to V200 of group B (p < 0.01); La1 of group A compared to La1 of group B (p < 0.01); LaR30 of group A compared to LaR30 of group B (p < 0.05). V200 represents the horse's cardiac power, thus it is possible that adult subjects, as 4 years (and older) horses, have perfectly developed the efficiency of the cardiac pump. Furthermore the galloper in its effort draws from the anaerobic metabolism which starts timely and to a significantly higher extent in older subjects (group B). This implies a greater efficiency of this metabolic pathway during exercise in these subjects. This could be indicative of the type of training done by the galloper. The results are discussed on the base of a possible use of a triangular exercise test on track for the functional assessment of the galloper. Keywords: exercise, anaerobic threshold, standardised test, blood lactate, heart rate, galloper Journal: Veterinární medicína Pages: 291-297 Volume: 49 Issue: 8 Year: 2004 DOI: 10.17221/5707-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5707-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200408-0003.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:8:id:5707-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Ozen Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: R. Ozturk Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: A. Yasar Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: A. Armutak Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: T. Basagac Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: A. Ozgur Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: I. Seker Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: H. Yerlikaya Author-Workplace-Name: 1Department of Veterinary History, Deontology and Ethics, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Title: An attitude of veterinary practitioners towards animal rights in Turkey Abstract: The authors examined the attitudes of veterinary practitioners in Turkey towards animals' right to life. For this purpose, a telephone questionnaire was applied on a total of 303 practitioners located in four provinces in Turkey. The overall response rate was 82%. The respect for right to life was valued slightly over neutral. According to the 5-point Likert scale, the average value scored by the participants for the items of views about animals' right to life was found as 3.25. Type of practice (pet clinics: 3.41), gender (females: 3.63), perceived responsibility (to animal: 3.48), keeping a pet (yes: 3.34) and membership in a society (yes: 3.67) had a statistically significant influence on attitudes towards animals' right to life. Independent variables explained 87% of the variance in attitudes, with most of the variance accounted for by perceived responsibility. Keywords: animal rights, animals' right to live, veterinarian, veterinary practitioners Journal: Veterinární medicína Pages: 298-304 Volume: 49 Issue: 8 Year: 2004 DOI: 10.17221/5708-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5708-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200408-0004.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:8:id:5708-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Ozbey Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Science, Author-Name: H. Ongor Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Science, Author-Name: D. T Balik Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Science, Author-Name: V. Celik Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Science, Author-Name: A. Kilic Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Science, Author-Name: A. Muz Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Science, Title: Investigations on ornithobacterium rhinotracheale in broiler flocks in elazig province located in the east of turkey Abstract: In the present study, lung, trachea and serum samples from broiler flocks slaughtered at an abattoir in Elazig province located in the East of Turkey were examined for the presence of Ornithobacterium rhinotracheale using culture and enzyme-linked immunosorbent assay (ELISA). The identity was latter proved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), western blot analysis, and polymerase chain reaction (PCR) assays. A total of 324 serum and 250 lung and trachea samples were collected from 10 commercially reared chicken flocks showing respiratory manifestations. The samples were obtained from different flocks. The causative agent (ORT) was isolated from trachea (1.5%) of five chickens and from both lung and trachea (0.4%) of only one chicken in the bacteriological examination of tissues. The presence of antibodies against ORT was detected in 33 (10.2%) of the 324 sera by ELISA. A 784 bp fragment of the 16S rRNA gene was amplified using specific primers in the PCR. All ORT isolates that were positive by culture were also detected to be positive by the PCR. SDS-PAGE protein profiles of whole cell extracts showed a high similarity for all the isolates with a major band of the molecular weight of 33 kDa (kiloDalton). Results of Western blot analysis indicate four antigenic fractions predominantly with molecular weights of 33, 42, 52 and 66 kDa. Keywords: Ornithobacterium rhinotracheale, ELISA, SDS-PAGE, western blot analysis, PCR, chicken Journal: Veterinární medicína Pages: 305-311 Volume: 49 Issue: 8 Year: 2004 DOI: 10.17221/5709-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5709-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200408-0005.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:8:id:5709-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Revajova Author-Workplace-Name: University of Veterinary Medicine, Kosice, Slovak Republic Author-Name: M. Levkut Author-Workplace-Name: University of Veterinary Medicine, Kosice, Slovak Republic Author-Name: M. Kozak Author-Workplace-Name: University of Veterinary Medicine, Kosice, Slovak Republic Author-Name: J. Bilek Author-Workplace-Name: University of Veterinary Medicine, Kosice, Slovak Republic Author-Name: D. Magic Author-Workplace-Name: University of Veterinary Medicine, Kosice, Slovak Republic Author-Name: R. Herich Author-Workplace-Name: University of Veterinary Medicine, Kosice, Slovak Republic Title: Canine histiocytic syndrome manifested as ulcerative gastroenterocolitis, skin lesions and lymphadenopathy - a case report Abstract: Histiocytic syndrome was diagnosed in a 7-year-old boxer bitch using histological and immunohistochemical methods. Necropsy confirmed the presence of enlarged superficial lymph nodes, two large ulcerated oval cutaneous masses one on the left lateral thoracic wall and one near to the vulva. In the gastrointestinal tract there were multiple ulcers apparently overlying nodules located in the submucosa of stomach, ulcers in the ileocaecal valve, and enlargement of lymphoid nodules in the small intestine manifested ulcerative gastroenteritis. Histologically, the thoracic wall mass showed infiltration of the tissue by macrophages with cytoplasmic vacuoles. The vacuoles contained PAS-positive polysaccharides. The macrophages were positive for alpha-1-antitrypsin and lysozyme by immunostaining. Lysozyme is a marker for phagocytic macrophages/histiocytes and may be used to confirm cells of this lineage in cases when there is any doubt. Keywords: dog, histiocytic disorder, skin, intestine, lymph nodes Journal: Veterinární medicína Pages: 312-316 Volume: 49 Issue: 8 Year: 2004 DOI: 10.17221/5710-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5710-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200408-0006.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:8:id:5710-VETMED