Template-Type: ReDIF-Article 1.0 Author-Name: J. Osek Author-Workplace-Name: Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Pulawy, Poland Title: Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs Abstract: A total of 90 Escherichia coli O157 isolates recovered from humans, cattle, and pigs, were examined for the presence of the H7 antigen, ability to produce Shiga toxins and enterohemolysin as well as for antimicrobial resistance and biochemical properties. Fourteen of the human strains (n = 23) and 21 of the bovine isolates (n = 29) were of the O157:H7 serotype as determined by agglutination and PCR methods. All E. coli O157 of porcine origin (n = 38) were H-negative. Based on the ability to produce Shiga toxins (Stxs), all human and cattle isolates and 11 strains recovered from swine were identified as Shiga toxin-producing E. coli (STEC). Among STEC, most human strains (18 isolates) were Stx1- and Stx2-positive whereas cattle strains were mostly Stx2-positive. Eleven porcine STEC produced either Stx1 (7 isolates) or Stx2 (4 strains) toxins; an additional 20 isolates recovered from these animals had the Stx2e toxin gene as previously determined by PCR. All human and cattle E. coli O157 produced enterohemolysin whereas only 4 strains recovered from pigs were ehly-positive. Moreover, the PCR identification of the lpfO113 gene performed earlier revealed that this putative virulence marker was present in all porcine isolates, only in 5 strains of bovine origin but in none of E. coli O157 recovered from humans. All 90 E. coli O157 strains tested displayed 10 biochemical profiles that were different at least in one of the reaction tested. The most common atypical reaction observed among porcine O157 isolates was ability to ferment sorbitol (all strains) and production of β-glucuronidase (25 isolates). Moreover, none of the sorbitol-positive strains was able to produce indol. Four antimicrobial resistance profiles among 90 E. coli O157 strains tested were observed. Most of the isolates recovered from humans and all strains from cattle were resistant only to rifampicin whereas the porcine strains showed resistance to either 3 antimicrobials (4 isolates) or to 4 drugs tested (34 isolates). The phenotypic data shown in the present study, together with the previously published genotypic analyses of these strains, confirm earlier suggestions that the porcine E. coli O157 strains are mostly different from those of bovine and human O157 isolates and could therefore play less important role in human STEC O157 infections. Keywords: E. coli O157, phenotypic properties, virulence marker genes, antibiotic resistence Journal: Veterinární medicína Pages: 317-326 Volume: 49 Issue: 9 Year: 2004 DOI: 10.17221/5711-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5711-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200409-0001.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:9:id:5711-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Ciftci Author-Workplace-Name: , A. C 2, O. E 2 1Ataturk University, Arts and Science Faculty, Biotechnology Application and Research Centre, Erzurum, Turkey Author-Name: A. Ciltas Author-Workplace-Name: , A. C 2, O. E 2 1Ataturk University, Arts and Science Faculty, Biotechnology Application and Research Centre, Erzurum, Turkey Author-Name: O. Erdogan Author-Workplace-Name: , A. C 2, O. E 2 1Ataturk University, Arts and Science Faculty, Biotechnology Application and Research Centre, Erzurum, Turkey Title: Purification and characterization of glucose 6-phosphate dehydrogenase from rainbow trout (oncorhynchus mykiss) erythrocytes Abstract: Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) from rainbow trout (Oncorhynchus mykiss) erythrocytes was purified, using a simple and rapid method, and some characteristics of the enzyme were investigated. The purification procedure consisted of three steps: haemolysate preparation, ammonium sulphate precipitation, and 2',5'-ADP Sepharose 4B affinity gel chromatography, which took one working day. Thanks to the three consecutive procedures, the enzyme, having the specific activity of 14.51 EU/mg proteins, was purified with a yield of 70.40% and 1 271.19-fold. In order to control the purification of the enzyme SDS polyacrylamide gel electrophoresis was carried out. SDS polyacrylamide gel electrophoresis showed a single band for the enzyme. Optimal pH, stable pH, optimal temperature, molecular weight, and KM and Vmax values for NADP+ and glucose 6- phosphate (G6-P) were also determined for the enzyme. In addition, the effect of NADPH on the enzyme was investigated and Ki value and the type of inhibition were determined by means of Lineweaver-Burk graph obtained for NADPH. Keywords: Keywords: purification, characterization, glucose 6-phosphate dehydrogenase, Oncorhynchus mykiss, erytrocyte Journal: Veterinární medicína Pages: 327-333 Volume: 49 Issue: 9 Year: 2004 DOI: 10.17221/5712-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5712-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200409-0002.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:9:id:5712-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Timurkaan Author-Workplace-Name: Department of Histology-Embryology, Faculty of Veterinary Science, University of Firat, Elazig, Turkey Author-Name: B. G Tarakci Author-Workplace-Name: Department of Histology-Embryology, Faculty of Veterinary Science, University of Firat, Elazig, Turkey Title: Immunohistochemical determination of calbindin-d28k in the kidney of postnatal rats Abstract: The localization of calbindin-D28k was examined in rat kidney at different stages of development. Calbindin was found specifically in the distal convoluted tubules (DCT). In the 10 day old rat kidney many cells apparently belonging to the collecting tubules were positive. In the neonatal nephrons there was also localization of calbindin-D28k to the limb of Henle, suggesting a difference in the regulation of intracellular calcium during maturation. Proximal tubule, renal corpuscle and macula densa gave no specific localization of calbindin-D28k. Keywords: immunohistochemistry, calbindin-D28k, kidney, rat Journal: Veterinární medicína Pages: 334-338 Volume: 49 Issue: 9 Year: 2004 DOI: 10.17221/5713-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5713-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200409-0003.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:9:id:5713-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Saroglu Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: K. Altunatmaz Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: Y. Devecioglu Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Title: A case of anterior iris cyst in an akbash dog Abstract: In this case presentation, an uncomplicated anterior iris cyst encountered in an 8-year-old female Akbash dog has been discussed. While these structures may be congenital, they may also develop secondarily due to trauma or chronic inflammation. Following systemic examination, the iris cyst was diagnosed to be present in the 5-6 o'clock position and that it was attached to the pupillary margin on the anterior surface of the iris. There were no abnormalities in any of the intraocular structures. No intervention of treatment was made, since the anterior iris cyst in the patient did not cause either corneal opacity or an increase in intraocular pressure. Keywords: iris cyst, uveal cyst, Anatolian Sheepdog, Akbash Journal: Veterinární medicína Pages: 339-342 Volume: 49 Issue: 9 Year: 2004 DOI: 10.17221/5714-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5714-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200409-0004.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:9:id:5714-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Novotny Author-Workplace-Name: , L. D 2, 3, A. L 2, V. B 2, I. P 2 1Institute of Pathological Morphology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: L. Dvorska Author-Workplace-Name: , L. D 2, 3, A. L 2, V. B 2, I. P 2 1Institute of Pathological Morphology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: A. Lorencova Author-Workplace-Name: , L. D 2, 3, A. L 2, V. B 2, I. P 2 1Institute of Pathological Morphology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: V. Beran Author-Workplace-Name: , L. D 2, 3, A. L 2, V. B 2, I. P 2 1Institute of Pathological Morphology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: , L. D 2, 3, A. L 2, V. B 2, I. P 2 1Institute of Pathological Morphology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Title: Fish: a potential source of bacterial pathogens for human beings Abstract: Human infections caused by pathogens transmitted from fish or the aquatic environment are quite common and depend on the season, patients' contact with fish and related environment, dietary habits and the immune system status of the exposed individual. They are often bacterial species facultatively pathogenic for both fish and human beings and may be isolated from fish without apparent symptoms of the disease. The infection source may be fish kept for both for food and as a hobby. Human infections and intoxications with the following bacteria have been recorded: Mycobacterium spp., Streptococcus iniae, Photobacterium damselae, Vibrio alginolyticus, V. vulnificus, V. parahaemolyticus, V. cholerae, Erysipelothrix rhusiopathiae, Escherichia coli, Aeromonas spp., Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, Clostridium botulinum, C. perfringens, Campylobacter jejuni, Delftia acidovorans, Edwardsiella tarda, Legionella pneumophila, and Plesiomonas shigelloides. Fish tissue histamine intoxications of people have frequently been described. The purpose of the present paper was to elaborate an overview of significant bacterial causative agents of human diseases transmitted from fish used as food or by handling them. Keywords: risk assessment, food safety, the CzechRepublic, mycobacteriosis, Mycobacterium avium complex Journal: Veterinární medicína Pages: 343-358 Volume: 49 Issue: 9 Year: 2004 DOI: 10.17221/5715-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5715-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200409-0005.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:9:id:5715-VETMED