Template-Type: ReDIF-Article 1.0 Author-Name: P. Alexa Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Hamrik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Stouracova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L. Konstantinova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: E. Salajka Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Passive immunoprophylaxis of edema disease in weaned piglets Abstract: Hyperimmune sera against Shiga toxin Stx2e with antibody titres 8 000-32 000, prepared by immunization of slaughter pigs, were administered subcutaneously to piglets (n = 73) 3 days after weaning. The titres of Stx2e neutralizing antibodies in sera of piglets ranged from 16 to 512 following application of the immune serum. In most of the piglets, passively acquired antibodies disappeared during 4 to 8 weeks after application. The recorded serum titres were in all of the piglets 8 or higher fourteen days following administration. In another experiment, an immune porcine serum with antibody titre against Stx2e of 130 000 was applied subcutaneously into a knee fold to 30 piglets. The serum was applied at the dose 3 ml, 1.5 ml, 0.75 ml, 0.4 ml, 0.2 ml, 0.1 ml, and 0.05 ml based on the group of piglets. Antibody-free serum was applied to pigs of the control group (n = 5). The following day, supernatant of STEC culture with Stx2e titre of 150 000 was applied to piglets of both experimental and control groups into the opposite knee fold that the above serum. Piglets that received 0.2 to 3 ml of serum remained unaffected throughout the experiment following application of supernatant containing Stx2e. Piglets that received 0.05 ml of serum and those of the control group showed severe clinical manifestations of edema disease following Stx2e application and were sacrificed ante finem. Marked signs of the disease could already be observed 18 hrs following Stx2e application. In a group of 3 piglets that received 0.1 ml of serum, mild paralysis was observed in 1 piglet 18 hrs following Stx2e application. Keywords: coliinfections, Escherichia coli, Shiga toxin, Stx2e, verotoxin, VT2e Journal: Veterinární medicína Pages: 447-452 Volume: 49 Issue: 12 Year: 2004 DOI: 10.17221/5738-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5738-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200412-0001.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:12:id:5738-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Hruban Author-Name: V. Horak Author-Name: K. Fortyn Author-Name: J. Hradecky Author-Name: J. Klaudy Author-Name: D. M Smith Author-Name: H. Reisnerova Author-Name: I. Majzlik Title: Inheritance of malignant melanoma in the melim strain of miniature pigs Abstract: Selective breeding of miniature pigs bearing cutaneous tumours resulted in the establishment of the MeLiM strain with hereditary malignant melanoma. The inheritance of melanoma was tested in a two-generation kindred comprising 456 progeny from 78 litters. Melanomas were recognisable visually as black-pigmented lesions on the skin. Their size, shape, number, progression and metastatic spreading varied widely. All possible melanoma forms known in human melanoma, i.e. pigmented naevi, dysplastic naevi, superficial spreading melanoma and nodular malignant melanoma, were found in the MeLiM pigs. Because the occurrence of nodular malignant melanoma segregated in all recorded litters we have included only this form in the genetic analysis. The tumours were nodular with exophytic growth over the skin surface. All showed similar histopathological features, vertical growth to muscle fascia and high metastatic activity. We hypothesize, on the basis of segregation ratios obtained from various mating types, that the mode of inheritance of nodular melanoma in the MeLiM strain is probably controlled by three genes. Keywords: heredity of porcine melanoblastoma, animal model, cutaneous tumour Journal: Veterinární medicína Pages: 453-459 Volume: 49 Issue: 12 Year: 2004 DOI: 10.17221/5739-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5739-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200412-0002.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:12:id:5739-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. M Al-Qudah Author-Workplace-Name: Department of Veterinary Clinical Sciences, Author-Name: L. A Sharif Author-Workplace-Name: Department of Veterinary Clinical Sciences, Author-Name: R. Y Raouf Author-Workplace-Name: Department of Veterinary Clinical Sciences, Author-Name: N. Q Hailat Author-Workplace-Name: Department of Veterinary Clinical Sciences, Author-Name: F. M Al-Domy Author-Workplace-Name: Department of Veterinary Clinical Sciences, Title: Seroprevalence of antibodies to chlamydophila abortus shown in awassi sheep and local goats in jordan Abstract: A cold complement fixation test (CFT) was used to identify C. abortus infection in ewes and does in northern Jordan. Sera from 36 flocks of sheep and 20 flocks of goats were collected randomly. The results showed that 433 (21.8%) out of 1 984 ovine sera, and 82 (11.4%) out of 721 caprine sera, were seropositive for C. abortus infection, as indicated by a titre ≥ 1:40. However, all the tested sheep flocks and goat flocks (100%) revealed at least one seropositive animal. There was a strong association (P < 0.05) between the rate of C. abortus infection and the size of the sheep flock, when larger flocks had higher infection rates. However, in goats, the flock size had no relationship with seropositivity. Age had no significant (P > 0.05) impact on C. abortus seropositivity. In sheep, there was a significant difference (P < 0.05) between the rates of the chlamydial infection in the four studied areas of northern Jordan. The highest infection rate in sheep (31.2%) was recorded in Mafraq area, while the rates in Irbid, Ajloun and Jerash were 18.5%, 11.2% and 13.9%, respectively. In goats, there was no significant difference between the rates of the chlamydial infection in the two areas studied. The rates of goat infections were 10.8% and 11.8% in Ajloun and Jerash areas, respectively. Keywords: seroprevalence, Chlamydophila abortus, sheep, goats Journal: Veterinární medicína Pages: 460-466 Volume: 49 Issue: 12 Year: 2004 DOI: 10.17221/5740-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5740-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200412-0003.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:12:id:5740-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Hamackova Author-Workplace-Name: , A. L 1, P. K 1, Z. S 2, J. K 1, P. L 1 1University of South Bohemia Ceské Budejovice, Research Institute of Fish Culture and Hydrobiology at Vodňany, Czech Republic Author-Name: A. Lepicova Author-Workplace-Name: , A. L 1, P. K 1, Z. S 2, J. K 1, P. L 1 1University of South Bohemia Ceské Budejovice, Research Institute of Fish Culture and Hydrobiology at Vodňany, Czech Republic Author-Name: P. Kozak Author-Workplace-Name: , A. L 1, P. K 1, Z. S 2, J. K 1, P. L 1 1University of South Bohemia Ceské Budejovice, Research Institute of Fish Culture and Hydrobiology at Vodňany, Czech Republic Author-Name: Z. Stupka Author-Workplace-Name: , A. L 1, P. K 1, Z. S 2, J. K 1, P. L 1 1University of South Bohemia Ceské Budejovice, Research Institute of Fish Culture and Hydrobiology at Vodňany, Czech Republic Author-Name: J. Kouril Author-Workplace-Name: , A. L 1, P. K 1, Z. S 2, J. K 1, P. L 1 1University of South Bohemia Ceské Budejovice, Research Institute of Fish Culture and Hydrobiology at Vodňany, Czech Republic Author-Name: P. Lepic Author-Workplace-Name: , A. L 1, P. K 1, Z. S 2, J. K 1, P. L 1 1University of South Bohemia Ceské Budejovice, Research Institute of Fish Culture and Hydrobiology at Vodňany, Czech Republic Title: The efficacy of various anaesthetics in tench (tinca tinca l.) related to water temperature Abstract: The effect of three different anaesthetics, 2-phenoxyethanol (0.6 ml/l), Propiscin (0.75 ml/l)and clove oil (0.033 ml/l), on adult tench (Tinca tinca L.) of mean body weight 260 g (66-583 g), of both sexes was tested at four different water temperatures (17.9; 20.4; 22.6 and 25.1°C). The time periods necessary for the induction of particular characteristic phases of anaesthetisation and recovery were evaluated. At all temperatures, the statistically longest induction of anaesthesia (p < 0.05) and longest recovery (p < 0.01) were registered with Propiscin. With clove oil and 2-phenoxyethanol, the time period necessary for induction of phase II b anaesthesia statistically declined (p < 0.05) with rising temperature, however, this phenomenon was not seen with Propiscin. Keywords: phase anaesthesia, recovery, 2-phenoxyethanol, Propiscin, clove oil Journal: Veterinární medicína Pages: 467-472 Volume: 49 Issue: 12 Year: 2004 DOI: 10.17221/5741-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5741-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200412-0004.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:12:id:5741-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Cetin Author-Workplace-Name: Department of Obstetrics and Gynaecology, Author-Name: S. Y Gurgoze Author-Workplace-Name: Department of Obstetrics and Gynaecology, Title: Uterine prolapse in a purebred Arabian mare: a case report Abstract: A 17-year-old purebred Arabian mare was presented with a history of uterine prolapse of 3-hour duration. The case occurred just after expulsion of foetal membranes in a mare aborting during the 7th month of pregnancy. During treatment the uterus was replaced to its normal position following epidural anaesthesia. No vulval sutures were inserted. There was no vaginal discharge during the postoperative period. Rectal examination conducted 5 days later revealed that rapid involution of the uterus occurred. Keywords: uterine, prolapse, mare Journal: Veterinární medicína Pages: 473-475 Volume: 49 Issue: 12 Year: 2004 DOI: 10.17221/5742-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5742-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200412-0005.txt Handle: RePEc:caa:jnlvet:v:49:y:2004:i:12:id:5742-VETMED