Template-Type: ReDIF-Article 1.0 Author-Name: K. Nechvatalova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Knotigova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Krejci Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Faldyna Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: E. Gopfert Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: P. Satran Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Toman Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Significance of different types and levels of antigen-specific immunity to Actinobacillus pleuropneumoniaeinfection in piglets Abstract: The aim of the study was to verify under experimental conditions the hypothesis that a reliable protection of piglets against Actinobacillus pleuropneumoniae (App) infection can be obtained by colostrum-derived antibodies and/or a low-dose infection from sows naturally infected with App. Twenty-eight piglets were allocated into four groups. Piglets of groups 1A and 1B were the progeny of App-seronegative sows, piglets of groups 2A and 2B originated from App-seropositive sows. At the age of 4 weeks, piglets in groups 1A and 2A were exposed to a low infection dose with the pathogenic strain of App serotype 9 (4 × 104 CFU/ml). At the age of 8 weeks, all four groups of piglets were exposed to the App serotype 9 challenge (2.5 × 107 CFU/ml). We compared the differences in clinical, radiological, pathological changes and cytological findings in blood and bronchoalveolar lavage samples between groups with different levels of antigen-specific immunity after challenge. Piglets that obtained colostrum-derived antibodies and experienced a low-dose App infection were best protected against App challenge. More apparent clinical and pathological changes were observed in groups protected with either only colostrum-derived antibodies or active immunity induced by a preceding low-dose infection. However, none of the types of protection prevented developing of the disease and characteristic changes in the lungs. Cell counts changes in blood and lavage gave evidence of only bacterial infection in progress; however, between-group differences were not marked. Antibodies (IgG, IgA) present in the respiratory tract at the time of challenge played a major role in protection against App infection. Keywords: bacterial infection, bronchoalveolar lavage, mucosal immunity, pneumonia, porcine, protection, respiratory tract Journal: Veterinární medicína Pages: 47-60 Volume: 50 Issue: 2 Year: 2005 DOI: 10.17221/5596-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5596-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200502-0001.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:2:id:5596-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Houserova Author-Workplace-Name: Department of Chemistry and Biochemistry, Author-Name: J. Hedbavny Author-Workplace-Name: Department of Chemistry and Biochemistry, Author-Name: D. Matejicek Author-Workplace-Name: Department of Chemistry and Biochemistry, Author-Name: S. Kracmar Author-Workplace-Name: Department of Animal Nutrition, Mendel University of Agriculture and Forestry, Czech Republic Author-Name: J. Sitko Author-Workplace-Name: Commenius Museum, Moravian Ornithological Station, Prerov, Czech Republic Author-Name: V. Kuban Author-Workplace-Name: Department of Chemistry and Biochemistry, Title: Determination of total mercury in muscle, intestines, liver and kidney tissues of cormorant (Phalacrocorax carbo), great crested grebe (Podiceps cristatus) and Eurasian buzzard (Buteo buteo) Abstract: The total mercury concentrations in four tissues (muscle, intestines, liver and kidney) of aquatic birds (cormorant - Phalacrocorax carbo and great crested grebe - Podiceps cristatus) and Eurasian buzzard (Buteo buteo) were determined by cold vapour atomic absorption spectrometry (CV-AAS) using an Advanced Mercury Analyser AMA 254. The results of the direct CV-AAS analyses of homogenised solid samples were in very good agreement with those obtained by CV-AFS and CV-AAS analyses after acid digestion. Mercury concentrations in the tested tissues of adult populations of great crested grebe and cormorant were nearly twice as high as in the Eurasian buzzard. Significantly higher mercury concentrations were found in the liver and kidney of the cormorant (7-times and 2-times, respectively) compared to great crested grebe. The highest mercury concentration (39.2 mg/kg DM) was found in liver of adult population of cormorant while the content of mercury in younger cormorants was approx. 6-times lower (5.8 mg/kg DM). The total mercury concentration in liver was 6-times higher (2-3-times in muscle and kidney) but 13-times lower then those of the cormorant population living in Japan (Tokyo, Lake Biwa) and in the United States (Nevada, Carson River), respectively. Keywords: total mercury, atomic spectrometry, bird tissues, muscle, intestines, kidney, liver, cormorant (Phalacro-corax carbo), great crested grebe (Podiceps cristatus), Eurasian buzzard (Buteo buteo) Journal: Veterinární medicína Pages: 61-68 Volume: 50 Issue: 2 Year: 2005 DOI: 10.17221/5597-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5597-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200502-0002.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:2:id:5597-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Erisir Author-Workplace-Name: Department of Biochemistry, Veterinary Faculty of Firat University, Elazig, Turkey Author-Name: E. Ercel Author-Workplace-Name: Province Veterinary Control Laboratory, Elazig, Turkey Author-Name: S. Yilmaz Author-Workplace-Name: Department of Biochemistry, Veterinary Faculty of Firat University, Elazig, Turkey Author-Name: S. Ozan Author-Workplace-Name: Department of Biochemistry, Veterinary Faculty of Firat University, Elazig, Turkey Title: Evaluation of optimal conditions for arginase activity in streptozotocin induced diabetic rats Abstract: The assay conditions needed to achieve maximal activity of liver and kidney arginase in diabetic and non-diabetic rats were investigated and compared. The physicochemical and kinetic properties of liver arginase in diabetic and control rats were very similar, those of kidney arginase were significantly different. It was found that preincubation temperature (68°C), preincubation period (20 min), optimum pH (10.1) of liver arginase and Km (3.2) for its substrate, L-arginine, did not change in diabetic and non-diabetic rats. As a consequence of diabetes, the optimum Mn2+ concentration for liver arginase only changed from 1 to 2 mM. Although the preincubation temperature and period for activation of kidney arginase in control rats was unnecessary, they were found to be 56ºC and 12 min in diabetic rats. The pH profile of arginase in kidney of diabetic rats was different from that of control rats. The Km value (6.7) of arginase for L-arginine in kidney is unchanged in diabetes whereas a marked decrease in Vmax was found. Optimum Mn2+ concentration (2 mM) for kidney arginase was unchanged in diabetes. The activity of arginase in liver of diabetic animals was higher 1.5 to 1.7 times than that of controls. Diabetes caused an about 53% decrease of arginase activity in kidney of female rats, 26% in that of males. These findings may suggest an idea that encoded arginases by separate gene loci may be affected differently by the pathological and hormonal status. Keywords: arginase, diabetes, physicochemical and kinetic properties Journal: Veterinární medicína Pages: 69-76 Volume: 50 Issue: 2 Year: 2005 DOI: 10.17221/5598-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5598-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200502-0003.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:2:id:5598-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Ozudogru Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Science, University of Ataturk, Erzurum, Turkey Author-Name: Z. Soyguder Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Science, University of Yuzuncu Yil, Van, Turkey Author-Name: G. Aksoy Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Science, University of Kafkas, Kars, Turkey Author-Name: H. Karadag Author-Workplace-Name: Department of Anatomy, Faculty of Veterinary Science, University of Yuzuncu Yil, Van, Turkey Title: A macroscopical investigation of the portal veins of the Van cat Abstract: In this study veins that constituted the portal vein were investigated in eight adult Van cats. The portal vein of the Van cat was composed of five peripheral branches which supplied the abdominal organs and two intrahepatic branches at the hepatic porta. The peripheral branches were cranial mesenteric, splenic, gastroduodenal, right gastric and cystic veins. The cranial mesenteric vein was the largest vessel that joined to the portal vein, and was constituted by the caudal pancreaticoduodenal, ileal, ileocolic and jejunal veins. The splenic vein was formed by the left gastric, left gastroepiploic, pancreatic and short gastric veins. The gastroduodenal vein was formed by the cranial pancreaticoduodenal and right gastroepiploic veins. The right gastric vein separately joined to the portal vein. The caudal mesenteric vein joined to the portal vein either alone or by a common trunk receiving either the caudal pancreaticoduodenal vein or ileocolic vein. The caudal mesenteric vein also opened rarely into the splenic vein. Intrahepatic branches were the right branch which gave off the ramus caudatus and ramus dexter lateralis, and the left branch which gave off the ramus dexter medialis, ramus quadratus, ramus sinister lateralis and ramus sinister medialis. Keywords: portal vein, cat, anatomy Journal: Veterinární medicína Pages: 77-84 Volume: 50 Issue: 2 Year: 2005 DOI: 10.17221/5599-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5599-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200502-0004.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:2:id:5599-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Hajurka Author-Workplace-Name: Clinic of Animal Obstetrics, Gynaecology and Andrology, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: V. Macak Author-Workplace-Name: Clinic of Animal Obstetrics, Gynaecology and Andrology, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: V. Hura Author-Workplace-Name: Clinic of Animal Obstetrics, Gynaecology and Andrology, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: L. Stavova Author-Workplace-Name: Clinic of Animal Obstetrics, Gynaecology and Andrology, University of Veterinary Medicine, Košice, Slovak Republic Author-Name: R. Hajurka Author-Workplace-Name: Clinic of Animal Obstetrics, Gynaecology and Andrology, University of Veterinary Medicine, Košice, Slovak Republic Title: Spontaneous rupture of uterus in the bitch at parturition with evisceration of puppy intestine - a case report Abstract: A rupture of one uterine horn at the region of small curvature and placentation was observed in a pregnant 8-year old Schnauzer bitch carrying a single foetus. It occurred at its second parturition. The first whelping at the age of 3 years had been without complications and the bitch reared eleven puppies. The uterine rupture occurred at the puppy's umbilical cord. An urgent obstetrical examination revealed one dead foetus in anterior presentation and ventral position. Caesarean delivery was indicated. Evisceration of puppy's intestine through its ruptured abdominal wall at the umbilical cord could be observed through the uterine wall rupture of 1.5 cm in diameter. The bitch underwent hysterectomy and recovered without complications. Keywords: bitch, rupture of uterus, parturition, single pup syndrome Journal: Veterinární medicína Pages: 85-88 Volume: 50 Issue: 2 Year: 2005 DOI: 10.17221/5600-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5600-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200502-0005.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:2:id:5600-VETMED