Template-Type: ReDIF-Article 1.0 Author-Name: K. Nedbalcova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: P. Satran Author-Workplace-Name: State Veterinary Administration of the Czech Republic, Department of Animal Health and Welfare Protection, Prague, Czech Republic Author-Name: Z. Jaglic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: R. Ondriasova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Kucerova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Monitoring of antibiotic resistance in isolates of Actinobacillus pleuropneumoniaein the Czech Republic between 2001 and 2003 Abstract: During the period of 2001 and 2003 a total of 238 isolates of Actinobacillus pleuropneumoniae obtained from 26 pig herds in the Czech Republic (2001 - 73 isolates, 2002 - 110 isolates, 2003 - 55 isolates) were examined for antibiotic resistance by disc diffusion method. Resistance to erythromycin (95.5% to 98.6%), streptomycin (76.4% to 82.7%) and tiamulin (72.7%) was detected most frequently over the whole monitored period. High increase in percentage of resistant isolates to tetracycline (2001 - 11.0%, 2002 - 36.4%, 2003 - 81.8%), doxycycline (2001 - 9.6%, 2002 - 34.6%, 2003 - 61.8%), nalidixic acid (2001 - 2.7%, 2002 - 14.5%, 2003 - 45.5%), and norfloxacin (2001 - 0%, 2002 - 7.3%, 2003 - 34.6%) was found, while differences in resistance to cephalotine (2001 - 1.4%, 2002 - 7.3%, 2003 - 9.1%), cotrimoxazol (2001 - 4.1%, 2002 - 10.0%, 2003 - 7.3%), amoxicillin (2001 - 12.3%, 2002 - 12.7%, 2003 - 10.9%) and amoxicillin-clavulanic acid (2001 - 9.6%, 2002 - 9.1%, 2003 - 10.9%) were small. Prevalence of isolates resistant to sulfasomidine was detected decreasing (2001 - 43.8%, 2002 - 47.3% and 2003 - 29.1%). Keywords: Actinobacillus pleuropneumoniae, porcine pleuropneumonia, serotypes, antibiotics, resistance, pig, Czech Republic Journal: Veterinární medicína Pages: 181-185 Volume: 50 Issue: 5 Year: 2005 DOI: 10.17221/5613-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5613-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200505-0001.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:5:id:5613-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Pavlata Author-Workplace-Name: Clinic of Diseases of Ruminants, Author-Name: S. Slosarkova Author-Workplace-Name: Department of Physiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Fleischer Author-Workplace-Name: Clinic of Diseases of Ruminants, Author-Name: A. Pechova Author-Workplace-Name: Clinic of Diseases of Ruminants, Title: Effects of increased iodine supply on the selenium status of kids Abstract: The aim of the study was to monitor the effect of increased iodine supplementation of the clinically healthy kid organism on the selenium status. The study included 7 clinically healthy 14-day-old kids from mothers with high iodine supplementation (group E) and 7 clinically healthy kids from mothers with hypoiodaemia (group C). Kids in group E were administered potassium iodide orally from 14 to 90 days of age. During the experimental period, the group E kids had a total daily iodine intake (from the feeding ration and from the per os potassium iodide administration) of 440-590 µg per head and day in comparison with 140-190 µg per head and day in the group C kids (only from the feeding ration; no potassium iodide administration). In kids of both groups, selenium concentration (Se), glutathione peroxidase activity (GSH-Px), concentration of the thyroid gland hormones (T3 and T4) and animal weights were monitored. In the group E kids, lower Se concentration (88.1 ± 10.9 µg/l; p < 0.01) and lower activity of GSH-Px (484.0 ± 125.4 µkat/l; p < 0.05) were proved at the end of the experiment (at 105 days of age of the kids) in comparison with the group C kids (131.8 ± 23.2 µg/l and 713.3 ± 153.3 µkat/l, respectively). No significant differences were found out in the T3 or T4 concentrations or in the weights of animals of both groups. The results indicate that increased iodine supplementation may have a negative effect on selenium metabolism and/or selenium status in kids. Keywords: goat, trace elements, interaction, glutathione peroxidase, thyroid hormones Journal: Veterinární medicína Pages: 186-194 Volume: 50 Issue: 5 Year: 2005 DOI: 10.17221/5614-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5614-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200505-0002.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:5:id:5614-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Prinosilova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Veznik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A. Zajicova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: D. Svecova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Using the Sperm Quality Analyzer (SQA IIc) to evaluate dog ejaculates Abstract: 38 fresh ejaculates were examined, of which 23 were cryopreserved in three diluents differing in concentration of glycerol and examined after thawing. For each ejaculate we established the concentration, motility, speed of sperm, percentage of live sperm and the morphological image of the ejaculate using routine laboratory diagnosis and SQA parameters at the initial value and after a 120-minute survival test. The values of the parameters found through routine diagnosis and using the SQA device were statistically confronted. From the results it emerges that the device functions on the basis of the turbulence effect of the semen, and therefore on the intensity and character of the moving mass of sperm. From a research perspective, the SQA device is less usable, since the values it provides are not absolutely comparable with the values found through routine laboratory diagnosis. For the practitioner, however, the device may be useful, since the value of the sperm motility index (SMI) indicates the quality of the semen through the close connection of two parameters, concentration and motility. For a more precise assessment of the ejaculate, however, it is necessary to also analyse the remaining parameters of routine laboratory sperm analysis. To be useful in clinical practice for the evaluation of semen, it would be necessary to establish SMI limit values for ejaculates of varying quality. Keywords: dog, ejaculate, sperm analysis, Sperm Quality Analyzer (SQA) Journal: Veterinární medicína Pages: 195-204 Volume: 50 Issue: 5 Year: 2005 DOI: 10.17221/5615-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5615-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200505-0003.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:5:id:5615-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: W.G. Nowak Author-Workplace-Name: BOKU Wien - University of Natural Resources and Applied Life Sciences, Vienna, Austria Title: A flexible and precise model for the dynamics of animal diseases with single outbreaks Abstract: This article provides a general and accurate mathematical model for the epidemics dynamics of a large class of animal diseases. The issue is to offer to the scientist interested in applications a quite concise and practicable explanation how to validitate the model parameters by means of the software tool Microsoft Excel®. Furthermore, predictions concerning hypothetical scenarii can easily be obtained on the basis of this concept. As specific examples, calculations are presented for the BSE epidemics in the British UK and for an outbreak of foot-and-mouth disease in a district of Lower Austria. Keywords: epidemics, animal diseases, mathematical model, BSE, foot-and-mouth disease Journal: Veterinární medicína Pages: 205-212 Volume: 50 Issue: 5 Year: 2005 DOI: 10.17221/5616-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5616-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200505-0004.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:5:id:5616-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Sychra Author-Workplace-Name: Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Chewing lice (Phthiraptera: Amblycera, Ischnocera) from chukars (Alectoris chukar) from a pheasant farm in Jinacovice (Czech Republic) Abstract: One hundred and twenty captive chukars (Alectoris chukar) and other hosts from a pheasant farm in Jinacovice (Czech Republic) were searched for chewing lice from July 2003 to April 2004. Seven species of chewing lice were found on chukars: Amyrsidea perdicis, Menacanthus pallidulus, Menopon gallinae (Amblycera); Goniodes colchici, Lipeurus maculosus, Goniocotes microthorax and Cuclotogaster heterographus (Ischnocera). Except for the last two mentioned species, the other five louse species are recorded for the first time on chukars. The prevalence, mean intensity of infestation and mean abundance of the lice from chukars were recorded. The species diversity and distribution of chewing lice from another eight species of gallinaceous birds kept in the pheasant farm were also recorded. Keywords: chewing lice, Phthiraptera, chukar, Alectoris chukar, pheasant farm Journal: Veterinární medicína Pages: 213-218 Volume: 50 Issue: 5 Year: 2005 DOI: 10.17221/5617-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5617-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200505-0005.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:5:id:5617-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Neronov Author-Workplace-Name: Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: P. Giurov Author-Workplace-Name: Institute of Experimental Pathology and Parasitology, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: M. Cholakova Author-Workplace-Name: Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: M. Dimitrova Author-Workplace-Name: Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, Bulgaria Author-Name: E. Nikolova Author-Workplace-Name: Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, Bulgaria Title: Cryoprotection of porcine cornea: a scanning electron microscopy study Abstract: Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5-10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures. Keywords: porcine cornea, Me2SO, glycerol, 1, 2-propanediol, PEG-400 Journal: Veterinární medicína Pages: 219-224 Volume: 50 Issue: 5 Year: 2005 DOI: 10.17221/5618-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5618-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200505-0006.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:5:id:5618-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Erdost Author-Workplace-Name: Department of Histology - Embryology, Faculty of Veterinary Medicine, University of Uludag, Gorukle, Bursa, Turkey Title: Immunohistochemical distribution of prolactin containing cells in the pituitary of the chickens Abstract: The objective of the current study was to determine the immunohistochemical distribution and density of prolactin containing cells in the pituitary of the female and male chickens during postnatal developing period after hatching for five months. The modified labelled avidin-biotin method with monoclonal mouse antihuman prolactin as a primary antibody was used to detect prolactin positive cells. At the end of each month, prolactin containing cells were identified in the pars distalis of the pituitary glands of chickens. The number of the prolactin positive cells were found to be increased depending on the monthly development period, while the reaction density was found to be decreased in all groups. Additionally, males had less prolactin positive cells than that of females. Keywords: pituitary, pars distalis, prolactin containing cells, chicken Journal: Veterinární medicína Pages: 225-229 Volume: 50 Issue: 5 Year: 2005 DOI: 10.17221/5619-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5619-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200505-0007.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:5:id:5619-VETMED