Template-Type: ReDIF-Article 1.0 Author-Name: K. Hruska Author-Workplace-Name: Veterinary Research Institute2, Brno, Czech Republic Author-Name: M. Bartos Author-Workplace-Name: Veterinary Research Institute2, Brno, Czech Republic Author-Name: P. Kralik Author-Workplace-Name: Veterinary Research Institute2, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute2, Brno, Czech Republic Title: Mycobacterium avium subsp. paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved Abstract: Fifty one products of dried milk baby food purchased from 10 producers from seven countries available on the Czech market have been tested. IS900, the specific fragments for Mycobacterium avium subsp. paratuberculosis (MAP) have been detected using PCR in 25 samples (49.0 %) and fragment f57 by real time PCR in 18 samples (35.3%). These results correspond to the epidemiological situation in Europe and are not unexpected. Paratuberculosis in cattle was almost unknown in the Czech Republic until 1990. An increase in the number of cows with paratuberculosis found in slaughterhouses and the incidence of Crohn's disease in the last decade is evident. The possible risk of MAP dead cells or bacterial structures in food is discussed in respect to autoimmune Crohn's disease. The national programmes of paratuberculosis control and certification of paratuberculosis-free herds should be strongly supported to decrease the risk for children and other people under higher risk. Producers should use MAP free milk for baby food production on a voluntary basis. Keywords: Johne's disease, paratuberculosis control, Crohn's disease, IS900, f57 Journal: Veterinární medicína Pages: 327-335 Volume: 50 Issue: 8 Year: 2005 DOI: 10.17221/5631-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5631-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200508-0001.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5631-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D.Y. Zhang Author-Workplace-Name: College of Life Science, Author-Workplace-Name: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Author-Name: J.Y. Zhou Author-Workplace-Name: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Author-Name: J. Fang Author-Workplace-Name: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Author-Name: J.Q. HU Author-Workplace-Name: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Author-Name: J.X. WU Author-Workplace-Name: Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Author-Workplace-Name: Institute of Biotechnology, Zhejiang University, Hangzhou, China Author-Name: A.X. MU Author-Workplace-Name: General Station of Veterinary Medicine, Hangzhou Bureau of Agriculture, Hangzhou, China Title: An ELISA for antibodies to infectious bronchitis virus based on nucleocapsid protein produced in Escherichia coli Abstract: The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in Chinawas expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency value was calculated in the N-ELISA to evaluate the antibody level of chicken serum. In an experiment to test field samples for antibody detection, the N-ELISA assay shared 95.7% identity of total positive ratio with the commercial ELISA kit. It indicated that the N-ELISA assay, which was safer and easier to prepare than traditional methods, was a good candidate for evaluation of IB vaccine efficiency and virus exposure. Keywords: infectious bronchitis virus, nucleocapsid protein, in vitro expression, ELISA Journal: Veterinární medicína Pages: 336-344 Volume: 50 Issue: 8 Year: 2005 DOI: 10.17221/5632-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5632-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200508-0002.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5632-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Jaglic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Kucerova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Nedbalcova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: P. Alexa Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Bartos Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Characterisation and comparison of Pasteurella multocida isolated from different species in the Czech Republic: capsular PCR typing, ribotyping and dermonecrotoxin production Abstract: The aim of this study was to characterise and compare Pasteurella multocida isolates originating from pigs (n = 43), calves (n = 31), rabbits (n = 27), and to a lesser extent from other hosts (n = 6). A total of 107 P. multocida isolates were obtained from various locations in the Czech Republic. They were analysed by capsular PCR typing and ribotyping, and tested for the production of dermonecrotoxin. Most frequently, serogroup A isolates (n = 74) were found, followed by serogroup D (n = 25) and serogroup F (n = 8) isolates. From a total of fifteen different ribotypes (1-15) generated by restriction endonuclease MspI, four ribotypes (1, 3, 4, and 7) were predominant. The prevalence of predominant ribotypes was different in isolates originating from different hosts. Ribotype 1 was characteristic for rabbit isolates, ribotype 3 was primarily found in pig isolates, and ribotype 7 dominated among calf isolates. Sixteen (mainly porcine) isolates produced dermonecrotoxin but significant correlation among ribotypes and dermonecrotoxin production was not observed. Keywords: pasteurellosis, serotyping, RFLP, pasteurella multocida toxin Journal: Veterinární medicína Pages: 345-354 Volume: 50 Issue: 8 Year: 2005 DOI: 10.17221/5633-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5633-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200508-0003.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5633-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Kucerova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Jaglic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: R. Ondriasova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Nedbalcova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Serotype distribution of Actinobacillus pleuropneumoniae isolated from porcine pleuropneumonia in the Czech Republicduring period 2003-2004 Abstract: A total of 245 isolates of Actinobacillus pleuropneumoniae included in this study were isolated from lung tissues of pigs which died of porcine pleuropneumonia. The isolates were obtained from 52 swine herds in the Czech Republic over the period 2003-2004. All the serotypes were serotyped by the coagglutination test. All isolates with cross-reactivity between serotypes 9 and 11 or 1, 9, and 11 in the coagglutination test were further serotyped by the quantitative coagglutination test. The results of serotyping showed that serotype 9 was dominant (46.5%) followed by serotypes 2 (18.5%), and 11 (14.2%). Two-way cross-reaction between serotypes 9 and 11 was found for 7.5% of the isolates. The serotypes 4, 5, 7, and 12 were found infrequently (2.4%). The isolates that could not be serotyped (11%) were classified by PCR typing system based on the apx and omlA genes into the serotype groups 1, 9, 11 (7.5%); 2, 8 (2.3%), and 7, 13 (1.1%). Keywords: pig, epidemiology, serotyping, quantitative coagglutination, polymerase chain reaction Journal: Veterinární medicína Pages: 355-360 Volume: 50 Issue: 8 Year: 2005 DOI: 10.17221/5634-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5634-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200508-0004.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5634-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Trckova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L. Matlova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: H. Hudcova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Faldyna Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Zraly Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: L. Dvorska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Beran Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Peat as a feed supplement for animals: a literature review Abstract: Peat is an easily available natural material and a source of biologically active substances widely used, not only in agriculture but in human and animal medicine as well. In recent years, interest in the use of peat as a feed supplement has increased, particularly due to its capability to prevent enteric diseases and to stimulate growth in piglets and pigs. The purpose of this review was to compare the advantages and risks associated with the use of peat for animal nutrition based on the literature available. Beneficial effects of various peat preparations on digestion, growth and the immune systems of animals as well as the absorbent and detoxifying capabilities are associated with the high content of favourable humic substances. One disadvantage of using peat preparations is the considerable diversity of the various types of peat caused by different biological, chemical and geological conditions during formation. Biological activity of various peat preparations is associated not only with fluctuations in the chemical compositions, but also with different application techniques. Based on the existing studies, it is unclear which application technique is most effective for respective animal species. Further studies should be conducted to elucidate the problem, with the inclusion of farm animals. One potential risk of peat feeding is the possibility of primary or secondary mycobacterial contamination. As long as feed rations are supplemented with peat preparations, it is essential to minimise the potential contamination risk during mining, processing and storage. Keywords: humate, humic substances, humic acid, fulvic acid, moor, mycobacteria, tuberculosis, Mycobacterium avium komplex Journal: Veterinární medicína Pages: 361-377 Volume: 50 Issue: 8 Year: 2005 DOI: 10.17221/5635-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5635-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200508-0005.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5635-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Yuksel Author-Workplace-Name: Department of Pathology, 3Department of Surgery, Faculty of Veterinary Medicine, Yuzuncu Yil University, Van, Turkey Author-Name: M.Y. Gulbahar Author-Workplace-Name: Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey Author-Name: L. Aslan Title: Congenital synchronous adenomas of meibomian and moll glands of the eyelid in a calf Abstract: This report describes meibomian adenoma (MA) and moll gland adenoma in a female, 1.5-months-old, crossbreed calf. Macroscopically, the mass was 3 × 1.5 × 0.5 cm in size and 5.35 g in weight; its shape was gray-reddish and it was localized on the right lower eyelid. Microscopically, the mass was composed of lobules of various sizes separated with a septum, which included well-differentiated sebaceous cells and apocrine secretory epithelium, with no an association of both gland types. Keywords: eyelid, meibomian gland, moll gland, adenoma, calf Journal: Veterinární medicína Pages: 379-383 Volume: 50 Issue: 8 Year: 2005 DOI: 10.17221/5636-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5636-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200508-0006.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:8:id:5636-VETMED