Template-Type: ReDIF-Article 1.0 Author-Name: P.D. Katsoulos Author-Workplace-Name: Scholar of the Greek State Scholarships Foundation, Author-Name: N. Roubies Author-Workplace-Name: Laboratory of Clinical Diagnosis and Clinical Pathology, Author-Name: N. Panousis Author-Name: E. Christaki Author-Name: P. Karatzanos Author-Name: H. Karatzias Title: Effects of long term feeding dairy cows on a diet supplemented with clinoptilolite on certain haematological parameters Abstract: The effect of the dietary inclusion of clinoptilolite on the haematological parameters of dairy cows has not been studied yet; however in mice, prolonged zeolite administration causes disturbances in the adaptation of erythropoiesis in periods of increased demands. The aim of the present study was to investigate whether the long term supplementation of two levels (1.25 and 2.5%) of clinoptilolite in the concentrate feed of dairy cows has any effect on their haematological parameters. Fifty-two clinically healthy Holsteincows were randomly assigned to one of three groups according to their age and parity. The first group (group A, n = 17) was offered a concentrate feed supplemented with 1.25% clinoptilolite. The second group (group B, n = 17) was offered a concentrate feed supplemented with 2.5% clinoptilolite. The third group (group C, n = 18), which served as control, was offered the same concentrate feed without clinoptilolite supplementation. The experiment started 30 days before the expected parturition and lasted up to the end of lactation. Blood samples from individual animals were collected just before the start of experiment, at the day of calving and, thereafter, at monthly intervals. All samples were tested for packed cell volume (PCV), hemoglobin (Hb) and leukocyte count (WBC) values. The results showed that the 1.25 and 2.5% supplementation of clinoptilolite had no adverse effect on the haematological parameters tested. Keywords: clinoptilolite, PCV, Hb, WBC, cows Journal: Veterinární medicína Pages: 427-431 Volume: 50 Issue: 10 Year: 2005 DOI: 10.17221/5644-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5644-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200510-0001.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:10:id:5644-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Uhlik Author-Workplace-Name: Department of Histology and Embryology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic Author-Name: V. Konradova Author-Workplace-Name: Department of Histology and Embryology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic Author-Name: L. Vajner Author-Workplace-Name: Department of Histology and Embryology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic Author-Name: J. Adaskova Author-Workplace-Name: Department of Applied Mathematics and Computer Science, Faculty of Science, Charles University, Prague, Czech Republic Title: Normobaric hypoxia induces mild damage to epithelium of terminal bronchioles in rabbits (ultrastructural study) Abstract: We studied the ultrastructure of the epithelium of terminal bronchioles in rabbits exposed for 96 hours to hypoxia (10% O2) in isobaric hypoxic chamber. Rabbits of the first control group (treated controls) were exposed for the same time in the same hypoxic chamber with atmosphere regulated at 21% O2. In both groups, the temperature in the chamber was 23°C and humidity 100% during the whole experiment. The second control group (untreated controls) was kept under standard conditions. The target cells for the effect of high temperature, humidity and normobaric hypoxia were the secretory elements. Both in hypoxic animals and treated controls, isolated Clara cells revealed signs of pathological alteration. More degenerative changes of Clara cells and marks of their compensatory proliferation were ascertained after exposure to hypoxia. Electron dense secretory granules were observed in most Clara cells. This finding reflected the initial stage of their secretory product formation. The secretory granules were usually stored in cytoplasm; morphological signs of their evacuation were found only exceptionally. The ciliated cells were less damaged than the secretory ones. On their apical surfaces, formation of cytoplasmic protrusions, which sometimes led to degeneration of free cilia, was ascertained. The damage of the epithelium and mild secretory stimulation of its secretory elements could play a role in the lung injury caused by the subacute normobaric hypoxia. Keywords: airways, Clara cells, ciliated cells, electron microscopy Journal: Veterinární medicína Pages: 432-438 Volume: 50 Issue: 10 Year: 2005 DOI: 10.17221/5645-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5645-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200510-0002.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:10:id:5645-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Kalender Author-Workplace-Name: Veterinary Control and Research Institute, Elazig, Turkey Author-Name: H.B. Ertas Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: B. Cetinkaya Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: A. Muz Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: N. Arslan Author-Workplace-Name: Veterinary Control and Research Institute, Elazig, Turkey Author-Name: A. Kilic Author-Workplace-Name: Veterinary Control and Research Institute, Elazig, Turkey Title: Typing of isolates of Clostridium perfringensfrom healthy and diseased sheep by multiplex PCR Abstract: In this study, C. perfringens strains isolated from healthy and diseased sheep were analysed by multiplex PCR in order to to detect the presence of the alpha, beta, epsilon, iota and enterotoxin genes. C. perfringens was isolated from 52 of 104 sheep with enterotoxemia signs and from 61 of 194 clinically healthy sheep. Genotyping of 52 strains from diseased sheep indicated that 33 (64%) were type A, 11 (21%) type D and 8 (15%) type C. Of 61 strains from healthy sheep, 58 (95%) were type A and 3 (5%) type D. The other types of C. perfringens were not detected, and none of the isolates contained the enterotoxin gene. This result indicates that the enterotoxin of C. perfringens does not play the important role in the occurence of enterotoxemia in sheep. Keywords: C. perfringens, sheep, toxin genes, PCR Journal: Veterinární medicína Pages: 439-442 Volume: 50 Issue: 10 Year: 2005 DOI: 10.17221/5646-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5646-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200510-0003.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:10:id:5646-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Hubalek Author-Workplace-Name: Medical Zoology Laboratory, Institute of Vertebrate Biology, Academy of Science of the Czech Republic, Brno, Valtice Workplace, Czech Republic Author-Name: V. Skorpikova Author-Workplace-Name: South-Moravian Regional Administrative Office, Brno, Czech Republic Author-Name: D. Horal Author-Workplace-Name: Agency for Nature Conservation and Landscape Protection of the Czech Republic, Brno, Czech Republic Title: Avian botulism at a sugar beet processing plant inSouth Moravia (Czech Republic) Abstract: An episode of mortality in waterbirds occurred on a sedimentation reservoir with effluents from the sugar beet processing plant at Hrusovany n.J. (South Moravia, Czech Republic) in summer 2003: tens of black-headed gulls (Larus ridibundus), several lapwings (Vanellus vanellus), one little ringed plover (Charadrius dubius), one ruff (Philomachus pugnax), one wood sandpiper (Tringa glareola), and two avocets (Recurvirostra avosetta) died. One of the two avocets and the plover, local breeders, were examined and found positive for Clostridium botulinum type C toxin by use of the toxin-neutralization test. Avocet is classified as a rare bird species according to Red Book data and is listed as critically endangered species in theCzechRepublic, and is therefore protected. Avian botulism can occasionally cause deleterious effects to waterbird fauna and its protection. Keywords: Clostridium botulinum, free-living birds, Charadriiformes, avocet, Recurvirostra avosetta, Charadrius dubius, Larus ridibundus Journal: Veterinární medicína Pages: 443-445 Volume: 50 Issue: 10 Year: 2005 DOI: 10.17221/5647-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5647-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200510-0004.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:10:id:5647-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Kul Author-Workplace-Name: Department of Pathology, 5Department of Basic Sciences, Faculty of Veterinary Medicine, Kirikkale University, Yahsihan, Kirikkale, Turkey Author-Name: R. Tunca Author-Workplace-Name: Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey Author-Name: R. Haziroglu Author-Workplace-Name: Department of Pathology, Author-Name: K.S. Diker Author-Workplace-Name: Department of Microbiology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: S. Karahan Title: An outbreak of avian tuberculosis in peafowl (Pavo cristatus) and pheasants (Phasianus colchicus) in a zoological aviary inTurkey Abstract: Avian tuberculosis was diagnosed histopathologically and microbiologically in two pheasants (Phasianus colchicus) and two peafowl (Pavo cristatus) kept in the same aviary. The incidence of avian tuberculosis in the aviary was 6%. Non-mineralized caseogranulomas were present in the liver (3 cases), spleen (3 cases), intestine (2 cases), lung (2 cases), and cloaca (1 case). Granulomas in the lung were present only in peafowl. The presence of granulomas in the lung of both infected peafowl suggests that peafowl were exposed to the agent via the respiratory route rather than the alimentary route. Histopathologic findings were typical of avian tuberculosis, including acid fast bacilli and centrally located caseo-necrosis surrounded by epitheloid macrophages, lymphocytes, and multinucleated giant cells. Mycobacterium avium subsp. avium was isolated from tissue samples of all infected birds. Keywords: avian tuberculosis, pathology, peafowl, pheasant, zoonosis Journal: Veterinární medicína Pages: 446-450 Volume: 50 Issue: 10 Year: 2005 DOI: 10.17221/5648-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5648-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200510-0005.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:10:id:5648-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B. Senturk Author-Workplace-Name: Department of Animal Health Research, General Directorate of Agricultural Researches, Ministry of Agriculture, Yenimahalle, Ankara, Turkey Author-Name: C. Yalcin Author-Workplace-Name: Department of Animal Health Economics and Management, Faculty of Veterinary Medicine, Ankara University, Diskapi, Ankara, Turkey Title: Financial impact of foot-and-mouth disease inTurkey: acquisition of required data viaDelphiexpert opinion survey Abstract: The main obstacle in assessing the financial impact of foot-and-mouth disease inTurkeyis unavailability of reliable data. Considering this issue, this study aimed at using a Delphi Expert Opinion Survey (DEOS) method to obtain data required for economic analysis of FMD inTurkey. This study concluded that although there were problems in obtaining some information from the experts, in general theDelphitechnique is a promising way of obtaining animal health data, which is otherwise missing and/or not regularly recorded in developing countries. Keywords: foot-and-mouth disease, Delphi, economic analysis, Turkey Journal: Veterinární medicína Pages: 451-460 Volume: 50 Issue: 10 Year: 2005 DOI: 10.17221/5649-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5649-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200510-0006.txt Handle: RePEc:caa:jnlvet:v:50:y:2005:i:10:id:5649-VETMED