Template-Type: ReDIF-Article 1.0 Author-Name: E. Skrivanova Author-Workplace-Name: Research Institute of Animal Production, Prague, Czech Republic Author-Name: M. Marounek Author-Workplace-Name: Research Institute of Animal Production, Prague, Czech Republic Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic Author-Name: V. Benda Author-Workplace-Name: Department of Biochemistry and Microbiology, Institute of Chemical Technology in Prague, Prague, Czech Republic Author-Name: P. Brezina Author-Workplace-Name: Department of Food Technology and Chemistry, Thomas Bata University in Zlin, Zlin, Czech Republic Title: Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringensto organic acids and monolaurin Abstract: The antimicrobial activity of fatty acids, monolaurin, citric, succinic, fumaric, malic and lactic acid was determined in cultures of two strains of Escherichia coli, three strains of Salmonella sp. and two strains of Clostridium perfringens. Antimicrobial activity was expressed as minimum inhibitory concentration (MIC) that prevented growth and glucose utilization in treated cultures. Caprylic acid was the only acid inhibiting glucose utilization in all cultures. Its MIC varied from 1 to 3 mg/ml. Strains CCM 3954 and CCM 4225 of E. coli were inhibited also by capric acid at 5 mg/ml. Strains CCM 4435T and CNCTC 5459 of Cl. perfringens were inhibited by medium-chain fatty acids (C8 to C14), oleic acid and one strain also by linoleic acid. The minimum MICs were those of lauric and myristic acid (between 0.1 and 0.2 mg/ml). Growth of Cl. perfringens, but not other bacteria, was inhibited also by monoglyceride of lauric acid (MIC = 3 mg/ml), and by citric acid (MIC = 4 mg/ml). Inhibitory effects of other acids were not observed at 5 mg/ml. Caprylic and lauric acid did not influence the K+ permeability of the cytoplasmic membrane in cells of E. coli CCM 4225 and Cl. perfringens CCM 4435T, respectively. In cultures of both strains of E. coli treated with caprylic acid at 5 mg/ml, and in those of Cl. perfringens CCM 4435T treated with lauric acid at 1 mg/ml, or with its monoglyceride at 5 mg/ml, the transmission electron microscopy revealed damage of cytoplasmatic structures. In cells of Cl. perfringens the separation of inner and outer membranes was apparent, the integrity of the outer membrane, however, was maintained. It can be concluded that medium-chain fatty acids are more efficient antimicrobials than other, more polar organic acids tested. Keywords: fatty acids, monolaurin, enteropathogenic bacteria, inhibition Journal: Veterinární medicína Pages: 81-88 Volume: 51 Issue: 3 Year: 2006 DOI: 10.17221/5524-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5524-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200603-0001.txt Handle: RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5524-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D. Horky Author-Workplace-Name: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: I. Lauschova Author-Workplace-Name: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic Author-Name: M. Klabusay Author-Workplace-Name: Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic Author-Name: M. Doubek Author-Workplace-Name: Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic Author-Name: P. Sheer Author-Workplace-Name: Department of Physiology and Pathophysiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: S. Palsa Author-Workplace-Name: Department of Internal Medicine - Haematooncology, Masaryk University Hospital, Brno, Czech Republic Author-Name: J. Doubek Author-Workplace-Name: Department of Physiology and Pathophysiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Title: Appearance of iron-labeled blood mononuclear cells in electron microscopy Abstract: Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes. Keywords: ultrastructure, iron nanoparticles, cultivation, animal model Journal: Veterinární medicína Pages: 89-92 Volume: 51 Issue: 3 Year: 2006 DOI: 10.17221/5525-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5525-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200603-0002.txt Handle: RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5525-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Travnicek Author-Workplace-Name: Department of Anatomy and Physiology of Farm Animals, Faculty of Agriculture, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Author-Name: V. Kroupova Author-Workplace-Name: Department of Anatomy and Physiology of Farm Animals, Faculty of Agriculture, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Author-Name: I. Herzig Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Kursa Author-Workplace-Name: Department of Anatomy and Physiology of Farm Animals, Faculty of Agriculture, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic Title: Iodine content in consumer hen eggs Abstract: This paper presents the latest information about the supply of iodine to meet its requirements in hens of laying type, on the basis of iodine content in egg yolk and about the importance of eggs as an iodine source in human nutrition. The Sandell-Kolthoff method was applied to determine iodine content in the yolk of eggs from 9 large flocks (54 eggs) and 16 small flocks (96 eggs) in 2004, and from 10 large flocks (135 eggs) and 15 small flocks (114 eggs) in 2005. Iodine content was also determined in the albumen of 70 eggs. In 2004, the iodine content in the yolk of eggs from large flocks was 1 014.1 ± 356.6 while in 2005 it amounted to 1 663.8 ± 1 179.7 µg/kg fresh matter (P < 0.01); the respective values for the yolk of eggs from small flocks in 2004 and 2005 were 307.1 ± 255.7 and 519.5 ± 508.2 µg/kg fresh matter (P < 0.01). Compared to 1996, in 2005, the iodine content in yolk increased by 123.7% in large flocks, and by 19.2% in small flocks. The iodine content in albumen was 16.2 ± 9.7 µg/kg fresh matter, and it accounted for 2.6-5.0% of the total iodine content in 1 egg. The correlation coefficient between iodine contents in yolk and albumen was r = 0.67. In the Czech Republic, 1 egg from large flocks contains on average 31.2 µg iodine while 1 egg from small flocks contains 10.0 µg. Eggs from large flocks cover 7-14% and from small flocks 2.2-4.4% of the daily iodine requirement in adults. Iodine concentrations exceeding 2 500 µg/kg yolk fresh matter in large flocks were measured between week 32 and 60 of the laying cycle and with daily intake of 0.116-0.132 mg iodine per hen. Keywords: iodine supplementation, yolk, albumen, iodine need Journal: Veterinární medicína Pages: 93-100 Volume: 51 Issue: 3 Year: 2006 DOI: 10.17221/5526-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5526-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200603-0003.txt Handle: RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5526-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Houserova Author-Workplace-Name: Department of Chemistry and Biochemistry, Author-Name: V. Kuban Author-Workplace-Name: Department of Chemistry and Biochemistry, Author-Name: P. Spurny Author-Workplace-Name: Department of Fishery and Hydrobiology, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: P. Habarta Author-Workplace-Name: Department of Chemistry and Biochemistry, Title: Determination of total mercury and mercury species in fish and aquatic ecosystems of Moravian rivers Abstract: Contents of total mercury and mercury species (methylmercury - MeHg, inorganic mercury - Hg2+) were determined in four Moravian rivers - Jihlava, Becva, Loucka and Dyje (Czech Republic). Five tissues (muscle, gills, liver, kidney and skin) of chub (Leuciscus cephalus), zoobenthos, sediments and water samples were analyzed. Time stability of samples was also tested. The highest levels of total mercury were determined in muscle tissues of all tested fish. Relative contents of MeHg in muscle tissues of fish ranged from 83.6% to 92.0% of the total mercury contents. The relative contents of MeHg in sediments and in zoobenthos samples correlate very closely (correlation coefficient -0.83). A considerably lower content of MeHg (1.3-11.4%) was found in river sediments compared with lakes. A comparison of observed sampling sites (Vladislav, Hrubsice) proved the adverse effect of industrial contamination on the water ecosystem ofJihlavaRiver and incomplete removal of mercury species in a sewage station Keywords: speciation, total mercury, methylmercury, inorganic mercury, chub (Leuciscus cephalus), Jihlava, Becva, Loucka, Dyje, muscle, gills, liver, kidney, skin, zoobenthos, sediment, water, sample stability Journal: Veterinární medicína Pages: 101-110 Volume: 51 Issue: 3 Year: 2006 DOI: 10.17221/5527-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5527-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200603-0004.txt Handle: RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5527-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D. Vnuk Author-Workplace-Name: Clinic of Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: D. Maticic Author-Workplace-Name: Clinic of Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M. Kreszinger Author-Workplace-Name: Clinic of Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: B. Radisic Author-Workplace-Name: Clinic of Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J. Kos Author-Workplace-Name: Clinic of Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M. Lipar Author-Workplace-Name: Clinic of Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: T. Babic Author-Workplace-Name: Clinic of Surgery, Orthopaedics and Ophthalmology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: A modified salvage technique in surgical repair of perineal hernia in dogs using polypropylene mesh Abstract: In 16 male dogs who suffered from perineal hernia, polypropylene mesh was used to close a defect in the pelvic diaphragm. Pelvic bone was drilled on the pelvic floor and mesh was sutured through holes by polypropylene suture. Strong pelvic diaphragm, good long-term results and time-sparing by this technique was achieved. Suture sinuses were developed in two dogs one month postoperatively. Objectives of this study were to describe a new alternative technique of perineal herniorraphy and postoperative possible complications. Weakness of internal obturator muscle flap is complication which can be observed during transposition of internal obturator muscle flap. This technique can be used when internal obturator muscle flap is weak like the operation of the first choice. Keywords: perineal hernia, dog, polypropilene mesh Journal: Veterinární medicína Pages: 111-117 Volume: 51 Issue: 3 Year: 2006 DOI: 10.17221/5528-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5528-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200603-0005.txt Handle: RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5528-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.R. Alam Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: S.Y. Heo Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: H.B. Lee Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: J.H. Kim Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: Y.J. Park Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: K.C. Lee Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: I.H. Choi Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: N.S. Kim Author-Workplace-Name: Center for Healthcare Technology Development, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Title: Preaxial longitudinal intercalary radial hemimelia in a dog: a case report Abstract: A 9-month-old 10 kg intact male crossbred dog was admitted to The Animal Medical Centre,ChonbukNationalUniversitywith congenitally malformed right forelimb. Physical exanimation of the limb showed varus deviation of the forepaw and flexion contracture of the interphalangeal joints. The survey radiographs revealed complete absence of the radius, poor congruency of the humeroulnar joint and 90° varus deviation of the ulnocarpal joint. A preaxial longitudinal intercalary radial hemimelia was diagnosed and the dog underwent 3 surgeries for correction of the deformity. During the first surgery, olecranon osteotomy and arthrodesis of the elbow were performed. Three months later, a second surgery was done to perform carpal panarthrodesis using bone plate and screws which was unsuccessful. Two weeks after, with a third surgery carpal arthrodesis was performed using acrylic frame, the distal portion of limb was straightened and the residual deviation of the forepaw was corrected. The surgical corrections resulted in satisfactory use of the limb and a better quality of life. Keywords: radius, hemimelia, arthrodesis, dog Journal: Veterinární medicína Pages: 118-123 Volume: 51 Issue: 3 Year: 2006 DOI: 10.17221/5529-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5529-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200603-0006.txt Handle: RePEc:caa:jnlvet:v:51:y:2006:i:3:id:5529-VETMED