Template-Type: ReDIF-Article 1.0 Author-Name: M. Svoboda Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: K. Nechvatalova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Krejci Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Drabek Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Ficek Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Faldyna Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: The absence of iron deficiency effect on the humoral immune response of piglets to tetanus toxoid Abstract: The aim of this study was to investigate an antibody response to tetanus toxoid in iron-deficient piglets. Ten piglets were not given any iron preparation until the age of 21 days. Ten piglets were given 200 mg Fe3+ dextran intramuscularly at the age of 3 days. All piglets were immunized with tetanus toxoid 14 days after birth. Reimmunization was performed 21 days after the first immunization. IgM and IgG titres were measured in weekly intervals after immunization. Haematological indices and iron concentration in the blood plasma of iron-deficient piglets characterized iron deficiency. The dynamic changes in IgM and IgG response to tetanus toxoid were similar in iron-deficient and iron-supplemented piglets and no significant differences in titres between the two groups were found in any period of the trial. Keywords: antibodies, immunity, titre Journal: Veterinární medicína Pages: 179-185 Volume: 52 Issue: 5 Year: 2007 DOI: 10.17221/2003-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2003-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200705-0001.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:5:id:2003-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Valocky Author-Workplace-Name: Department of Gynaecology, Obstetrics and Andrology, Author-Name: J. Legath Author-Workplace-Name: Department of Toxicology, Author-Name: L. Lenhardt Author-Workplace-Name: Department of Pathological Anatomy, University of Veterinary Medicine, Kosice, Slovak Republic Author-Name: G. Lazar Author-Workplace-Name: Department of Gynaecology, Obstetrics and Andrology, Author-Name: F. Novotny Author-Workplace-Name: Department of Gynaecology, Obstetrics and Andrology, Title: Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls Abstract: The objective of this study was to examine the alkaline, acidic phosphatase and nonspecific esterase activity in the epithelial cells of oviducts after exposure to polychlorinated biphenyls (PCBs) at the time of puerperium. PCBs were administered in the last days of pregnancy and during early puerperium. Animals in the experimental group were exposed to Delor 105 at a dose of 100 μg/kg/day and were euthanised on Day 17 postpartum (n = 4), i.e. 5 days after the termination of 30-day PCB administration; on Day 25 postpartum (n = 5), i.e. 17 days from the last PCB administration and on Day 34 postpartum (n = 5), which corresponded to Day 28 from the completion of PCB administration. Ewes in the control group were euthanised on Day 17 (n = 3), Day 25 (n = 4) and Day 34 (n = 4) postpartum. The authors demonstrated the inhibitory effect of PCB on the enzymatic system of the oviduct during the puerperal period. The alkaline phosphatase, acidic phosphatase and nonspecific esterase activity in the oviductal epithelial cells during a 34-day observation period exhibited a rising trend (P < 0.001 vs. P < 0.001 vs. P < 0.01) in the control group of animals. Experimental animals exposed to the 30-day PCB administration (Delor 105) showed a stagnant tendency (P > 0.05) in alkaline phosphatase while acidic phosphatase and nonspecific esterase activity (P > 0.05) dropped even below the level of their activity values in the control group. It is essential to continue to monitor the effect of pollutants in exposed industrial areas on reparative and regenerative processes in puerperium and their possible impact on reproductive performance. Keywords: oviduct, puerperium, ewe, alkaline phosphatase, acidic phosphatase, nonspecific esterase, polychlorinated Journal: Veterinární medicína Pages: 186-192 Volume: 52 Issue: 5 Year: 2007 DOI: 10.17221/2004-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2004-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200705-0002.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:5:id:2004-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Tomasek Author-Workplace-Name: Avian and Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: O. Kubicek Author-Workplace-Name: National Authority for Nuclear, Biological and Chemical Protection, Brno, Czech Republic Author-Name: V. Tukac Author-Workplace-Name: Avian and Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction Abstract: High mortality of nestling cockatiels (Nymphicus hollandicus) was observed in one breeding flock in Slovakia. The nestling mortality affected 50% of all breeding pairs. In general, all the nestlings in affected nests died. Death occurred suddenly in 4- to 6-day-old birds, most of which had full crops. No feather disorders were diagnosed in this flock. Two dead nestlings were tested by nested PCR for the presence of avian polyomavirus (APV) and Chlamydophila psittaci and by single-round PCR for the presence of beak and feather disease virus (BFDV). After the breeding season ended, a breeding pair of cockatiels together with their young one and a fledgling budgerigar (Melopsittacus undulatus) were examined. No clinical alterations were observed in these birds. Haemorrhages in the proventriculus and irregular foci of yellow liver discoloration were found during necropsy in the young cockatiel and the fledgling budgerigar. Microscopy revealed liver necroses and acute haemolysis in the young cockatiel and confluent liver necroses and heart and kidney haemorrhages in the budgerigar. Two dead cockatiel nestlings, the young cockatiel and the fledgling budgerigar were tested positive for APV, while the cockatiel adults were negative. The presence of BFDV or Chlamydophila psittaci DNA was detected in none of the birds. The specificity of PCR was confirmed by the sequencing of PCR products amplified from the samples from the young cockatiel and the fledgling budgerigar. The sequences showed 99.6-100% homology with the previously reported sequences. To our knowledge, this is the first report of APV infection which caused a fatal disease in parent-raised cockatiel nestlings and merely subclinical infection in budgerigar nestlings. Keywords: budgerigar fledgling disease, Melopsittacus undulatus, parrots, viruses, PCR Journal: Veterinární medicína Pages: 193-201 Volume: 52 Issue: 5 Year: 2007 DOI: 10.17221/2002-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2002-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200705-0003.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:5:id:2002-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.R. Alam Author-Workplace-Name: Department of Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: J.I. Lee Author-Workplace-Name: Department of Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: H.B. Lee Author-Workplace-Name: Department of Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: J.J. KO Author-Workplace-Name: Department of Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: K.C. Lee Author-Workplace-Name: Department of Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: N.S. Kim Author-Workplace-Name: Department of Surgery, College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Title: Supernumerary ectopic limbs in Korean indigenous cattle: four case reports Abstract: Four head of Korean indigenous cattle were presented to the Chonbuk Animal Medical Centre, Chonbuk National University, with the complaint of the presence of extra limb(s) since birth. The accessory limb(s) were underdeveloped in all the cases and attached to the lateral side of the withers craniodorsal to the right scapula in Case 1 and 2; in Case 3, right dorsally to the 4th cervical vertebra and in Case 4 it was ventrolateral to the thorax caudally to the right forelimb. In all the cases, the congenital anomaly was diagnosed as the presence of supernumerary ectopic limb(s) (SEL), which were removed by surgical excisions under general anaesthesia. In all the cases, the surgical corrections resulted in normal locomotion and better quality of life. No postoperative complications were observed during a 1-year follow up period. The SEL can be successfully removed by surgical excisions, which can result in normal locomotion and better aesthesis of the animal. Keywords: supernumerary limbs, polydactyly, congenital anomaly, cattle Journal: Veterinární medicína Pages: 202-206 Volume: 52 Issue: 5 Year: 2007 DOI: 10.17221/2005-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2005-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200705-0004.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:5:id:2005-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Melter Author-Workplace-Name: Labvet, Clinical Veterinary Laboratory, Prague, Czech Republic Author-Workplace-Name: National Institute of Public Health, Prague, Czech Republic Author-Name: I. Stehlik Author-Workplace-Name: Veterinary Clinic, Prague, Czech Republic Author-Name: H. Kinska Author-Workplace-Name: Labvet, Clinical Veterinary Laboratory, Prague, Czech Republic Author-Name: I. Volfova Author-Workplace-Name: Labvet, Clinical Veterinary Laboratory, Prague, Czech Republic Author-Name: V. Ticha Author-Workplace-Name: Labvet, Clinical Veterinary Laboratory, Prague, Czech Republic Author-Name: D. Hulinska Author-Workplace-Name: National Institute of Public Health, Prague, Czech Republic Title: Infection with Anaplasma phagocytophilum in a young dog: a case report Abstract: An 11-months-old male Golden Retriever occasionally found to have Ixodes ricinus ticks attached to the skin developed the acute onset of fever, lameness and inappetence followed by rapidly progressive depression, ataxia and reluctance to move. Inclusions (morulae) were observed in granulocytes. The blood analysis revealed severe thrombocytopoenia, lymphopoenia, eosinopoenia, elevation of alkaline phosphatase and hypercholesterolaemia, mostly suggestive of an Anaplasma phagocytophilum infection. The amplification of a DNA sequence specific for Anaplasma phagocytophilum and detection of specific antibodies supported the diagnosis. Borrelia burgdorferi, another tick-borne pathogen, or specific antiborrelial IgG antibodies were not detected. The dog was treated with oral doxycycline for 14 days: clinical symptoms resolved within six days. Keywords: anaplasmosis, infection, dog Journal: Veterinární medicína Pages: 207-212 Volume: 52 Issue: 5 Year: 2007 DOI: 10.17221/2001-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2001-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200705-0005.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:5:id:2001-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Saroglu Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: D. O. Erdikmen Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: O. Guzel Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Author-Name: D. Aydin Author-Workplace-Name: Surgery Department, Faculty of Veterinary Medicine, Istanbul University, Avcilar, Istanbul, Turkey Title: Luxatio lentis in dogs: a case report Abstract: The material of the present study was composed of 30 eyes with luxatio lentis occurring in 20 dogs. Unilateral lens luxation was determined in a half of the animals while bilateral luxation was determined in the other half. Lenses in 19 of the 30 eyes were luxated in the anterior direction (63.3%), three were luxated in the posterior direction (10%) and eight were subluxated (26.6%). The distribution of patient dogs based on the breeds showed that the incidence of lens luxation was higher in Terrier, Cocker Spaniel and crossbreed dogs compared to the other breeds. The results of etiological classification of the diseases which are generally seen in older dogs (on average 7.2 years old) were as follows: congenital in two patients, primary luxation in four patients, and secondary luxation in 14 patients. Secondary luxations diagnosed in 14 animals were determined to have developed as a result of trauma in two dogs, glaucoma in one dog, uveitis in one dog, and senile degeneration and/or cataract in 10 dogs. Bilateral (two dogs) and unilateral (five dogs) intracapsullar lens extraction (ICLE) was applied to these animals. The primary disease was to be kept under control by treating those with secondary lens luxation for uveitis or glaucoma. A severe progressive intraocular inflammation developed in one patient. Enucleation was conducted on this patient due to buphthalmus developing in a short time. These results may be helpful to small animal veterinarians dealing with this disease which results in blindness unless early diagnosis and surgical treatment are conducted. Keywords: lens displacement, luxatio lentis, dog Journal: Veterinární medicína Pages: 213-222 Volume: 52 Issue: 5 Year: 2007 DOI: 10.17221/2006-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2006-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200705-0006.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:5:id:2006-VETMED