Template-Type: ReDIF-Article 1.0 Author-Name: Z. Veznik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: D. Svecova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: A. Zajicova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Reckova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Rubes Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: The interrelationship between quality parameters of sperm before and after separation by gradient centrifugation Abstract: Significance of a short-time survival test for the prediction of quality parameters of sperm obtained by gradient centrifugation was evaluated from an aspect of their functional resistance. Frozen semen samples from 27 bulls before and after separation were assessed by a 120-min survival test. The evaluation of morphological examination of ejaculates was carried out using the SASMO computer program. Statistically significant interrelationship between sperm quality parameters in the initial insemination doses, the amounts of obtained sperm after separation and their functional and vital resistance in the survival test was documented. Keywords: bull, sperm, qualitative separation Journal: Veterinární medicína Pages: 423-429 Volume: 52 Issue: 10 Year: 2007 DOI: 10.17221/2051-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2051-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200710-0001.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2051-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Moravkova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Bartos Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Workplace-Name: Veterinary and Pharmaceutical University, Brno, Czech Republic Author-Name: L. Dvorska-Bartosova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Beran Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Parmova Author-Workplace-Name: State Veterinary Institute, Prague, Czech Republic Author-Name: M. Ocepek Author-Workplace-Name: Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: M. Pate Author-Workplace-Name: Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Genetic variability of Mycobacterium avium subsp. avium of pig isolates Abstract: The genetic diversity of 132 pig isolates of Mycobacterium avium subsp. avium (MAA) from the Czech Republic, the Slovak Republic and Slovenia was examined by IS901 restriction fragment length polymorphism (RFLP) analysis with restriction endonuclease PvuII. A total of 18 RFLP types were detected. The occurrence frequency of respective RFLP types varied between respective pig farms, with the exception of one RFLP type F found in 21 (34.4%) of 61 farms and in 10 (55.6%) of 18 farms in the Czech Republic and Slovak Republic, respectively. Two different RFLP types were detected in 5 (33.3%) of the 15 studied farms, from which more than one isolate were examined. These results show the low variability of the MAA isolates among the pig farms and the possibility of various sources of infection for pigs from infected farms. Keywords: avian tuberculosis, food safety, molecular epidemiology, mycobacteriosis, zoonosis Journal: Veterinární medicína Pages: 430-436 Volume: 52 Issue: 10 Year: 2007 DOI: 10.17221/2048-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2048-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200710-0002.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2048-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Fleige Author-Workplace-Name: Physiology Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Freising, Germany Author-Name: W. PREIßINGER Author-Workplace-Name: Institute of Animal Nutrition and Feed Management, Bavarian Regional Office for Agriculture, Poing-Grub, Germany Author-Name: H.H.D. Meyer Author-Workplace-Name: Physiology Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Freising, Germany Author-Name: M.W. Pfaffl Author-Workplace-Name: Physiology Weihenstephan, Center of Life and Food Sciences, Technical University of Munich, Freising, Germany Title: Lactulose: effect on apoptotic- and immunological-markers in the gastro-intestinal tract of pre-ruminant calves Abstract: The study was conducted to elucidate the effects of orally administered lactulose in combination with Enterococcus faecium on immune response of the intestinal tract in pre-ruminant calves. The mRNA expression of pro- and anti-inflammatory cytokines and proliferation and apoptosis markers were investigated in jejunum, ileum, colon and caecum. Simmental calves were fed diets containing 1% (L1) or 3% (L3) lactulose and the probiotic strain of the genus E. faecium, and compared with a non treated control group. Primarily the high dose feeding with lactulose showed an effect on several mRNA gene expression parameters. In the jejunum a down-regulation of the anti-apoptotic marker Bcl-xl was determined and IL-10 mRNA gene expression was 2.6-fold up-regulated (P < 0.05). In the colon a 1.9-fold (P < 0.05) up-regulation of IL-10 and only in caecum an about 2-fold increase of TGF-β1 (P < 0.05) was found for both lactulose feedings. Caspase 3 was up-regulated in caecum only in the 3% lactulose treated group (P < 0.05). The enhanced apoptotic rate of caspase 3 seems to be associated with a decrease in crypth depth due to lactulose supplementation. The results indicated that mainly the high 3% lactulose dose in probiotic-fed calves has an affect on the intestinal immune function and on diverse apoptotic markers. Keywords: intestine, morphology, health Journal: Veterinární medicína Pages: 437-444 Volume: 52 Issue: 10 Year: 2007 DOI: 10.17221/2046-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2046-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200710-0003.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2046-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V.B. Kandimalla Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: N. Kandimalla Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Hruska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Franek Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay Abstract: During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. Hence, cost effective and simple screening methods are required for residual analysis of environmental and food samples on-site. Here, a simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase tracer conjugate, blocking agents and incubation times. Test results assessed by visual measurement can be available within 20 minutes. In buffer, water, skimmed milk and pig manure extract, sulfamethazine fortified at 50 and 100 µg/l has exhibited clear visual differentiation in colour development (lower intensity) in comparison to the control spot intensity (high intensity) of the dipstick. Keywords: antibody, sulfonamides, residues Journal: Veterinární medicína Pages: 445-450 Volume: 52 Issue: 10 Year: 2007 DOI: 10.17221/2045-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2045-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200710-0004.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2045-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Treml Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: J. Pikula Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: H. Bandouchova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: J. Horakova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: European brown hare as a potential source of zoonotic agents Abstract: There has recently been a growing interest in checking the state of health of European brown hares in hunting grounds because they are a susceptible bio-indicator of environmental changes and because of the dramatic decline in populations across Europe. A total of 1 051 (384, 302, and 365, respectively) blood sera were collected from hares during autumn hunting events and examined for tularaemia, brucellosis and leptospirosis in 2004, 2005 and 2006. Antibodies were found in 6.5, 1.6 and 7.5% of cases, respectively. Antibodies against Francisella tularensis, Brucella suis and leptospires were found in 7.9, 1.7 and 7.7% of females and 4.5, 1.4 and 7.9% of males, respectively. Higher seroprevalence of tularaemia was found in females (P = 0.05). Brucellosis was more prevalent in adult animals compared to subadult ones (P = 0.05). Only the L. grippotyphosa serotype was found and the titres mostly ranged from 100 to 400 (89.9%). Higher titres from 800 to 3 200 were found in the remaining 10.1% of the examined European brown hare sera. Antibodies against F. tularensis as well as B. suis were most frequent in low titres of 10 to 80. Higher titres were only exceptional. Confirming that hares are susceptible to various zoonotic agents, it is necessary to be careful when handling the animals killed. On the other hand, blood sera from hares may be used to survey the occurrence of natural nidi of zoonoses in hunting grounds. Keywords: Lepus europaeus, zoonosis, leptospirosis, tularaemia, brucellosis, antibodies Journal: Veterinární medicína Pages: 451-456 Volume: 52 Issue: 10 Year: 2007 DOI: 10.17221/2050-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2050-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200710-0005.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2050-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Gavrilovic Author-Workplace-Name: Department of Aquaculture, University of Dubrovnik, Dubrovnik, Croatia Author-Name: E. Srebocan Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J. Pompe-Gotal Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Z. Petrinec Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: A. Prevendar-Crnic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Z. Matasin Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Spatiotemporal variation of some metal concentrations in oysters from the Mali Ston Bay, south-eastern Adriatic, Croatia - potential safety hazard aspect Abstract: The concentrations of cadmium, lead and zinc were determined in the soft tissue of oysters (Ostrea edulis L.) from three locations in the Mali Ston Bay on the south-eastern coast of the Adriatic Sea. The sampling was performed during two periods of breeding cycle, in summer (age ~1 year) and winter (age ~20 months, consumption size). The concentrations of cadmium, lead and zinc measured in the oyster soft tissue showed considerable spatiotemporal variations that could be attributed to seasonal differences in the freshwater inflow which varied between the study locations. Median concentrations measured at different locations and in different time periods ranged from 0.303 to 0.969, 0.13 to 0.32 and 208.9 to 650.0 µg/g wet weight for cadmium, lead and zinc, respectively. Although metal concentrations do not exceed the maximal legally approved limits according to the legislation of the Republic of Croatia and EU, more knowledge of their spatiotemporal distribution - of cadmium in particular - would contribute to the sustainable future development of oyster farming in Croatian waters. Keywords: cadmium, lead, zinc, Ostrea edulis L Journal: Veterinární medicína Pages: 457-463 Volume: 52 Issue: 10 Year: 2007 DOI: 10.17221/2047-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2047-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200710-0006.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2047-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.E. Garcia Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Author-Name: P. Lanzarot Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Author-Name: V.L. Rodas Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Author-Name: E. Costas Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Author-Name: J.L. Blanco Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Title: Fungal flora in the trachea of birds from a wildlife rehabilitation centre in Spain Abstract: In the present work we study the prevalence of fungal flora in the tracheal epithelium of wild birds in a rehabilitation centre. Two hundred and sixteen birds representing 26 species from seven orders were sampled. Yeasts and moulds were isolated from 92 of the birds sampled (42.5%); in 24.5% only yeasts, in 12.5% only moulds and in 5.5% both moulds and yeasts together. The cattle egret was where the greatest number of animals with positive isolation was detected. The prevalence of yeasts shows significant differences between raptors and Ciconiiformes. Within the yeast flora, basically there were strains belonging to the genus Candida, mainly C. albicans. In the moulds, almost a half of them belonged to the genus Aspergillus. Keywords: birds, yeasts, moulds, fungi, wildlife, Candida, Aspergillus Journal: Veterinární medicína Pages: 464-470 Volume: 52 Issue: 10 Year: 2007 DOI: 10.17221/2049-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2049-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200710-0007.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:10:id:2049-VETMED