Template-Type: ReDIF-Article 1.0 Author-Name: E. Sudova Author-Workplace-Name: University of South Bohemia, Ceske Budejovice, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic Author-Name: J. Machova Author-Workplace-Name: University of South Bohemia, Ceske Budejovice, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic Author-Name: Z. Svobodova Author-Workplace-Name: University of South Bohemia, Ceske Budejovice, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: T. Vesely Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Negative effects of malachite green and possibilities of its replacement in the treatment of fish eggs and fish: a review Abstract: Malachite green has been used as an effective compound to control external fungal and protozoan infections of fish since 1933 but it has never been registered as a veterinary drug for use in food fish because of its potential carcinogenicity, mutagenicity and teratogenicity in mammals. The present paper reviews negative side-effects of malachite green including its accumulation and persistence in fish that have been treated and describes other alternative substances for the treatment of fish and fish eggs. Keywords: malachite green, fish baths, toxicity, accumulation, residues in fish, aquatic environment, protozoan ectoparasites, fungal infections Journal: Veterinární medicína Pages: 527-539 Volume: 52 Issue: 12 Year: 2007 DOI: 10.17221/2027-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2027-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200712-0001.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:12:id:2027-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Lopatarova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: S. Cech Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Krontorad Author-Workplace-Name: BOVET Company, Sloupnice, Czech Republic Author-Name: L. Holy Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: J. Hlavicova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Dolezel Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Lower quality bovine embryos may be successfully used for sex determination Abstract: The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used high quality (HQ) as well as lower quality (LQ) embryos obtained from superovulated donors. The healthy embryonic cells isolated from HQ embryos and blastomeres protruding into the perivitelline space of LQ embryos, were analysed by polymerase chain reactions (PCR) using primers specific for the Y chromosome determinant. After microsurgical intervention and completion of sex determination, the female embryos were then transferred (ET) to synchronized recipients. A total of 310 embryos of HQ were used and gender was safely determined in 275 cases (88.7%). PCR analysis of extruded cells isolated from 170 LQ embryos was carried out with certainty only in 111 embryos (65.3%, P < 0.01). After ET of 122 HQ sex defined embryos, pregnancy was established in 69 recipients (56.6%). A similar conception rate 51.9% (27/52) was found after the ET of sex defined embryos designated as LQ. The accuracy of analysis was confirmed after calving and revealed that designated female sex coincided with 95.5% and 96.2% of calves when HQ and LQ embryos were transferred, respectively. Our results clearly show that a microsurgical technique in combination with PCR method represents a rapid and reliable approach for sex determination in HQ as well as in LQ preimplantation bovine embryos and can be used in field conditions for the regulation of the sex of progeny in selected herds. Keywords: cattle, embryo, superovulation, sex determination, polymerase chain reaction, embryo transfer Journal: Veterinární medicína Pages: 540-546 Volume: 52 Issue: 12 Year: 2007 DOI: 10.17221/1884-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1884-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200712-0002.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:12:id:1884-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Zelenka Author-Workplace-Name: Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: J. Hedbavny Author-Workplace-Name: Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic Title: Effect of age upon retention of mercury in chickens with a different growth rate Abstract: Retention of total mercury was examined in groups of 95 slowly-growing laying type chickens and 52 fast-growing broiler hybrids in 47 subsequent balance periods from 1 to 100 days of age. Chickens were fed ad libitum on a diet containing 5.41 µg of Hg per kilogram. Until Day 4 the values of coefficients of apparent retention of Hg were negative or very low. They were influenced by excretion of endogenous nutrients originating primarily from the yolk sac. When evaluating the period of 4 to 100 days of age, the dependence of Hg retention coefficients upon age was highly significant (P < 0.01). The course of this dependence was expressed by parabolas for laying type chickens with the maximum value at Day 61 of age and for broilers with the minimum value at Day 53. In fast growing chickens, Hg content in body gain decreased until Day 14 and then increased while in slow growing chickens it was linearly increasing for the whole experimental period. The average coefficients of Hg retention from the feed mixture were 48.18 ± 0.719 (mean ± SEM) and 47.90 ± 1.057% and Hg retained per g of live body gain were 7.494 ± 0.4682 and 6.775 ± 0.6233 ng in laying and meat type cockerels, respectively. The difference between hybrids was insignificant (P > 0.05). The growth rate of total amount of Hg in the body was lower (P < 0.01) than that of body weight of chickens, allometric coefficients were 0.706 and 0.747 for slow and fast growing chickens, respectively. Keywords: poultry, deposition of mercury, allometry of growth Journal: Veterinární medicína Pages: 547-551 Volume: 52 Issue: 12 Year: 2007 DOI: 10.17221/1885-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1885-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200712-0003.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:12:id:1885-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Marounek Author-Workplace-Name: Institute of Animal Science, Prague-Uhrineves, Czech Republic Author-Workplace-Name: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic Author-Name: V. Skrivanova Author-Workplace-Name: Institute of Animal Science, Prague-Uhrineves, Czech Republic Author-Name: A. Dokoupilova Author-Workplace-Name: Institute of Animal Science, Prague-Uhrineves, Czech Republic Author-Name: M. Czauderna Author-Workplace-Name: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna, Poland Author-Name: A. Berladyn Author-Workplace-Name: Institute of Biotechnology, Lvov National Academy of Veterinary Medicine, Lvov, Ukraine Title: Meat quality and tissue fatty acid profiles in rabbits fed diets supplemented with conjugated linoleic acid Abstract: In this study the deposition of dietary CLA isomers in loin and hindleg meat, liver and fat, and the influence on performance and fatty acid (FA) profile were investigated in growing rabbits. CLA was supplied as synthetically produced oil at 5 and 10 g/kg diet for the whole fattening period (six weeks) or three weeks before the slaughter. CLA had no or limited effect on feed intake, growth, carcass traits and composition of meat. Treatment with CLA increased the proportion of saturated FA at the expense of monounsaturated FA in meat and liver. Supplementation of the diet with CLA increased (P < 0.05) CLA in lipids of meat from < 1 mg/g FA up to 36 mg/g FA. Adipose and hepatic tissue incorporated the highest (44 mg/g FA) and the lowest (14 mg/g FA) amount of CLA, respectively. The concentration of CLA in tissue lipids increased (P < 0.05) with increasing CLA content in the diet. Duration of CLA feeding had no effect on CLA deposition. Thus, dietary inclusion of CLA at higher concentration (10 g/kg) and feeding CLA-supplemented diet for a shorter period seems to be more suitable for production of CLA-containing rabbit meat. The ratio of the two most abundant isomers of CLA, cis-9, trans-11 and trans-10, cis-12 in tissues differed from that in the CLA-enriched diet. In all tissues the relative proportion of the former isomer was lower than in the diet. The experiment demonstrated that feeding synthetic CLA to rabbits is a means of enriching rabbit meat with CLA, which could provide a healthier product for human consumption. Keywords: rabbits, conjugated linoleic acid, fatty acids, meat quality, performance Journal: Veterinární medicína Pages: 552-561 Volume: 52 Issue: 12 Year: 2007 DOI: 10.17221/1886-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1886-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200712-0004.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:12:id:1886-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D. Pokorova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Piackova Author-Workplace-Name: University of South Bohemia, Ceske Budejovice, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic Author-Name: A. Cizek Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: S. Reschova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Hulova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Vicenova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: T. Vesely Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Tests for the presence of koi herpesvirus (KHV) in common carp (Cyprinus carpio carpio) and koi carp (Cyprinus carpio koi) in the Czech Republic Abstract: An outbreak of koi herpesvirus (KHV) infection associated with high mortality of common and koi carp was recorded in the USA and Israel in 1998. At present, this disease is viewed as one of the most significant factors that can adversely affect common and koi carp breeds. The disease has spread worldwide including European countries neighbouring with the Czech Republic (CR), i.e. Germany, Poland, and Austria. To monitor the situation in the CR, samples were collected from a total of 138 common and koi carps in seven and eight locations in CR respectively, and were examined between 2005 and 2006. Locations owned by the major producers of common and koi carp were selected with respect of potential occurrence of the KHV virus. No records of increased mortality and morbidity were noticed there. Preferentially carps with non-specific symptoms of disease were sampled, often with isolated skin erosions. To obtain detailed picture about health condition of examined fish the bacteriological and haematological examinations were accomplished. The next part of the examined samples were carp and koi carp for export from professional breeders, imported koi carp and fish from breeds with increased mortality (45 and 21 fish in 2005 and 2006, respectively) The only virological testing was done in this case. The culture and PCR method, according to Gilad et al. (2002) showed negative results for virus KHV in all years. Retrospective investigation by PCR method according to Bercovier et al. (2005) showed positivity in five locations in 2005. In 2006, KHV virus was not detected in any of the selected locations. The aim of our study was to find out the presence/absence of KHV in selected locations and potential correlation of virological, bacteriological and haematological findings. The results of first testing for presence of koi herpesvirus indicates the necessity of regular KHV monitoring in the Czech Republic in the next period. Keywords: koi, common carp, herpesvirus, koi herpesvirus (KHV) Journal: Veterinární medicína Pages: 562-568 Volume: 52 Issue: 12 Year: 2007 DOI: 10.17221/1883-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1883-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200712-0005.txt Handle: RePEc:caa:jnlvet:v:52:y:2007:i:12:id:1883-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: editors Title: VOLUME 52 - CONTENTS, AUTHOR INDEX, AUTHOR INSTITUTION INDEX, LIST OF REVIEWERS Journal: Veterinární medicína Pages: 569-577 Volume: 52 Issue: 12 Year: 2007 DOI: 10.17221/1887-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1887-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:52:y:2007:i:12:id:1887-VETMED