Template-Type: ReDIF-Article 1.0 Author-Name: M.E. Garcia Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Author-Name: J. Caballero Author-Workplace-Name: Laboratorios Hipra, Amer, Girona, Spain Author-Name: S. Alvarez-Perez Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Author-Name: J.L. Blanco Author-Workplace-Name: Veterinary Faculty, Complutense University, Madrid, Spain Title: Seroprevalence of Aspergillus fumigatus antibodies in bovine herds with a history of reproductive disorders Abstract: Bovine reproductive disorders are of growing interest from both economic and health standpoints. The role that fungi play in these processes is becoming increasingly important. Study of the seroprevalence of anti-Aspergillus antibodies in cow sera taken from herds with previous reproductive disorders is therefore of great interest. We used an indirect ELISA technique, that had shown suitable results in previous studies in other animal species. Thirteen percent of the 387 animals tested in the current study were positive, accounting for 41% of herds. When this technique was applied to the sera of two fetuses, that were aborted due to aspergillosis, a significant difference in anti-Aspergillus antibody levels was found compared with those in control sera. The ELISA technique was compared with PLATELIA and PCR for 24 ELISA-positive cattle sera; these assays resulted in only one and three positive results, respectively. In conclusion, the ELISA analysis showed a significant seroprevalence of Aspergillus antibodies in herds with previous reproductive disorders. There is therefore a need for more attention to be paid to Aspergillus in the differential diagnoses of these conditions and to improve preventive methods to effectively control this organism. Keywords: Aspergillus, abortion, fungi, ELISA, PCR, antibodies Journal: Veterinární medicína Pages: 117-123 Volume: 53 Issue: 3 Year: 2008 DOI: 10.17221/1939-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1939-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200803-0001.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:3:id:1939-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B. Pisarikova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Zraly Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: F. Bunka Author-Workplace-Name: University of Tomas Bata, Zlin, Czech Republic Author-Name: M. Trckova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Nutritional value of white lupine cultivar Butan in diets for fattening pigs Abstract: The aim of our study was to assess the nutritional value of whole and dehulled ground seeds of Lupinus albus L., cv. Butan by the determination of balance digestibility of nutrients in growing pigs at different levels of replacement of soy protein with lupine. The experiment was performed on 25 pigs of hybrid combination Pietrain × (Duroc × Large White × Landrace) with initial body weights of 30.7 ± 2.21 kg. The feed compound of the control group contained extracted soybean meal (ESM). In four experimental diets, 50% and 100% replacement of soy protein with the inclusion of whole (WL 50, WL 100) or dehulled (DL 50, DL 100) lupine was carried out. Nutrient and amino acid digestibility was determined using the indicator of insoluble ash in 4M HCl in pigs with a mean live body weight of 48.9 ± 3.51 kg . Higher digestibility of crude protein (P < 0.01), ether extract (P < 0.01), crude fibre (P < 0.01), NDF, ADF (P < 0.05), and cellulose (P < 0.05) was found for the diet WL 50 compared with the control group ESM. In the diet DL 50, higher digestibility of crude protein, ether extract, crude fibre, NDF, and cellulose (PP < 0.01) was recorded compared with the control. Higher digestibility of lysine and threonine (P < 0.01) was found in the diet WL 50 in comparison with ESM; a lower digestibility of methionine (P < 0.01) was found in the diet DL 50. Lower digestibility of crude protein, NDF, ADF (P < 0.01), and cellulose (P < 0.01) was found at 100% supplementation of soy protein with whole seed lupine (WL 100) compared to the control. A 100% replacement of soy protein with dehulled lupine (DL 100) resulted in significantly higher digestibility of dry matter (P < 0.01), crude protein (P < 0.05), ether extract (P < 0.01), crude fibre (P < 0.01), N-FE (P < 0.01), organic matter (P < 0.01), NDF (P< 0.01), ADF, and cellulose (P < 0.01) compared with the control diet. In this case, digestibility of threonine (P < 0.01) and lysine was higher, that of methionine was lower. The highest concentration and daily output of diaminopimelic acid (DAPA) were observed in the diet WL 100. Daily gains in live body weight were nonsignificantly higher in all experimental animals compared with the controls, the most beneficial conversion being a 100% replacement of soy protein with dehulled lupine (DL 100). Keywords: lupine, dehulled, nutrients, amino acids, digestibility, diaminopimelic acid, growth Journal: Veterinární medicína Pages: 124-134 Volume: 53 Issue: 3 Year: 2008 DOI: 10.17221/1943-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1943-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200803-0002.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:3:id:1943-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Podlasz Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo, Poland Author-Name: K. Wasowicz Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo, Poland Title: Neurochemical characteristics of paracervical ganglion in the pig Abstract: A study on the presence of the selected biologically active substances in nerve structures of the paracervical ganglion in the pig was performed with the use of immunofluorescence and RT-PCR. Immunohistochemical methods revealed that 23% of paracervical ganglion (PCG) neurons contain both tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DβH) and that the remaining 77% contain choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT). 73% of TH/DβH neurons contained neuropeptide Y (NPY) and 8% contained somatostatin (Som). All ChAT/VAChT positive neurons contained vasoactive intestinal polypeptide (VIP), 87% of them contained Som, 76% contained NPY and 32% contained neuronal nitric oxide synthase (nNOS). Galanin (Gal) was found only in small cells, which were thought to be SIF cells. No pituitary adenylate cyclase-activating polypeptide (PACAP)- or substance P (SP)-positive neurons were found in PCG. Some areas of PCG contained dense plexuses of ChAT- and VAChT-positive nerve fibres. In the ganglion small number of TH-, nNOS-, NPY-, VIP-, Gal-, PACAP-, Som- and SP-positive nerve fibres was also visible. RT-PCR detected the presence of mRNA for TH, ChAT, nNOS, NPY, VIP, Gal and Som, which were visualised as clearly discernible bands on a gel. In cases of PACAP and SP only weak bands were observed. Keywords: pig, paracervical ganglion, immunohistochemistry, RT-PCR Journal: Veterinární medicína Pages: 135-146 Volume: 53 Issue: 3 Year: 2008 DOI: 10.17221/1940-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1940-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200803-0003.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:3:id:1940-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Hesta Author-Workplace-Name: Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Author-Name: S. Arnouts Author-Workplace-Name: INVE Technologies, Dendermonde, Belgium Author-Name: G.P.J. Janssens Author-Workplace-Name: Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium Title: Dietary supplementation of coated butyrate in healthy dogs: effect on apparent digestibility, faecal flora and faecal volatile fatty acids Abstract: The aim of the present study was to determine the effect of dietary addition of coated butyrate on gut health related faecal traits in dogs. A food with or without coated butyrate was tested in a crossover study with 17 dogs. Faecal samples were collected and frozen until analysis for proximate components. Fresh faecal samples were collected for bacterial culturing and short chain fatty acids (SCFA) analysis. The addition of coated butyrate to a dog diet had neither effect on faecal consistency score, dry matter content and faecal production nor on digestibility coefficients. Absolute and relative faecal contents of SCFA were not different for the two foods. The bacterial nitrogen (N) content of the faeces was significantly lower after butyrate addition compared to the control diet. In conclusion, the addition of butyrate to a dog food had no effect on faecal characteristics but the faecal bacterial protein content was significantly reduced by butyrate addition. Keywords: butyric acid, faecal flora, digestibility, dog Journal: Veterinární medicína Pages: 147-152 Volume: 53 Issue: 3 Year: 2008 DOI: 10.17221/1941-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1941-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200803-0004.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:3:id:1941-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Dembovska Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: P. Svoboda Author-Workplace-Name: Emergency Hospital, Brno, Czech Republic Author-Name: P. Scheer Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: I. Kantorova Author-Workplace-Name: Emergency Hospital, Brno, Czech Republic Author-Name: J. Tomenendalova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: K. Rehakova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Author-Name: J. Doubek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science, Brno, Czech Republic Title: Model of septic shock induced by live E. coli (O18) in a laboratory rat Abstract: This study was concerned with the development of induced septic shock in a laboratory rat using a series of measurements including body temperature, heart and respiratory rates, haematocrit value, red and white blood cell counts, differential leukocyte count, haemoglobin value, glycaemia, analysis of arterial blood gases, and serum levels of interleukin 6 (IL-6) during the first five hours. A total of 12 specific pathogen free (SPF) laboratory rats were used for the study. Septic shock was induced under general anaesthesia by introducing live E. coli (O18) into the jugular vein in the dose of 1 × 109 per 100 g of body weight (group SESH). Clinical measurements and blood collection from a. carotis were performed just prior to, and then 1.5 and 5 h after the administration of E. coli. The control group (C) contained 9 SPF laboratory rats which received physiological saline only, at the same volume into the jugular vein, and blood collection followed according to the same scheme as above described for group SESH. The results of the experiment showed that changes in clinical, haematological and biochemical parameters could be detected as early as 1.5 hours after induction. These changes correspond with the activation of an inflammatory reaction and the development of metabolic acidosis. They are accompanied by a considerable rise in IL-6 already 1.5 h after the application of live E. coli and after 5 h the levels exceeded 2 000 pg/ml in all experimental animals. Our results clearly document the importance of IL-6 for the early detection of developing septic shock and of some less specific but routinely determined parameters such as white blood cell count and base excess. Keywords: sepsis, interleukin 6, systemic inflammatory response syndrome Journal: Veterinární medicína Pages: 153-164 Volume: 53 Issue: 3 Year: 2008 DOI: 10.17221/1942-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1942-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200803-0005.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:3:id:1942-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: W. Perez Author-Workplace-Name: Veterinary Faculty, University of the Republic of Uruguay, Montevideo, Uruguay Author-Name: M. Lima Author-Workplace-Name: Veterinary Faculty, University of the Republic of Uruguay, Montevideo, Uruguay Author-Name: G. Pedrana Author-Workplace-Name: Veterinary Faculty, University of the Republic of Uruguay, Montevideo, Uruguay Author-Name: F. Cirillo Author-Workplace-Name: Zoological Garden "Villa Dolores", Montevideo, Uruguay Title: Heart anatomy of Giraffa camelopardalis rothschildi: a case report Abstract: In the present study the most outstanding anatomical findings of the heart of a giraffe are described. Two papillary muscles were found in the right ventricle, namely magnus and subarterial. There were no papillary parvi muscles. The supraventricular crest gave insertion to various tendinous chords. These chords fixed the angular cusp of the right atrioventricular valve. The pectinate muscles were better developed in the left auricle than in the right one. Within the left ventricle two big papillary muscles were found as well as a notorious septomarginal trabecula. The left coronary artery irrigated the majority of the heart's territory. It gave origin to the interventricular paraconal branch and to the circumflex branch. The latter gave off the branch of the left ventricular border and the interventricular subsinosal branch. Keywords: animal anatomy, heart anatomy, wild animals, Giraffidae, coronary vessels Journal: Veterinární medicína Pages: 165-168 Volume: 53 Issue: 3 Year: 2008 DOI: 10.17221/1937-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1937-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200803-0006.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:3:id:1937-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.K. Borku Author-Workplace-Name: Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: M. Guzel Author-Workplace-Name: Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey Author-Name: M.C. Karakurum Author-Workplace-Name: Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey Author-Name: K. Ural Author-Workplace-Name: Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey Author-Name: S. Aktas Author-Workplace-Name: Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey Title: Nimesulide-induced acute biliary tract injury and renal failure in a kitten: a case report Abstract: A 3-month-old male kitten was presented to our clinic with malaise, vomiting and jaundice. In the anamnesis, we learned that the cat had a history of anorexia, sneezing, and nasal discharge and that the owner had administered 100 mg/day (t.i.d.) nimesulide orally for three days. In the laboratory study, high levels of serum alkaline phosphatase, γ-glutamyl transtransferase, total bilirubin, direct bilirubin, indirect bilirubin, urea, and creatinine were detected. All the clinical signs and laboratory abnormalities returned to normal levels after cessation of the nimesulide and supportive treatment. In this case, clinical and laboratory findings were thought to be compatible with nimesulide-induced acute biliary injury and renal failure. This case report indicates that the household pets are at risk of toxic drugs administered by their owners and great caution should be taken in administering NSAIDs in cats. Keywords: nonsteroidal anti-inflammatory drug, nimesulide, biliary injury, renal failure, kitten Journal: Veterinární medicína Pages: 169-172 Volume: 53 Issue: 3 Year: 2008 DOI: 10.17221/1938-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1938-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200803-0007.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:3:id:1938-VETMED