Template-Type: ReDIF-Article 1.0 Author-Name: B. Skotarczak Author-Workplace-Name: Department of Genetics, University of Szczecin, Poland Title: Babesiosis as a disease of people and dogs. Molecular diagnostics: a review Abstract: Babesia is the causative agent of babesiosis, a tick-borne zoonosis which has been increasingly described throughout the world. Babesia microti and Babesia divergens are the etiological agents of human babesiosis. Babesia canis is the principal etiological agent of canine babesiosis. Currently, the diagnostics of babesiosis is based mainly on serological methods and the immunofluorescent antibody test (IFA) is most commonly used. However, even in the acute phase of the disease, seroconversion does not always occur. Clinical symptoms, because of their unspecificity, cannot be used to make a correct diagnosis. In this situation other diagnostic methods are needed. The use of PCR (polymerase chain reaction) is the most promising of these. An advantage of this method is that it allows identification of the parasite in the early stage of disease which enables early diagnosis, implementation of therapy and avoidance of complications. However, the standardization of this technique remains to be carried out. Selection of a genetic marker for PCR is very important for the sensitivity of this technique and it is discussed in this paper. Keywords: babesiosis in dogs and humans, diagnostics, PCR, selection of markers to PCR Journal: Veterinární medicína Pages: 229-235 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1946-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1946-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0001.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1946-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Dvorakova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Prodelalova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Reichelova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Comparative inactivation of Aujeszky's disease virus, Porcine teschovirus and Vesicular stomatitis I by chemical disinfectants Abstract: We tested the germicide activity of 1% Chloramin BM, 1% Incidin Plus, 1% Lysoformin 3000, 0.2% Mikasept KP, and 2% Sekusept Forte against viruses in suspension (suspension test) and dried onto a surface (carrier test). The agents of the porcine encephalomyelitis (Porcine teschovirus, strains CAPM V-86, CAPM V-37), Aujeszky's disease (strains CAPM V-166, CAPM V-327) and vesicular stomatitis (strains CAPM V-499, CAPM V-331) were used as model viruses. After 30 min contact time in both the suspension and carrier tests, the Porcine teschovirus was 4 lg inactivated only by Mikasept, which was thus the only disinfectant to meet the standard. The other disinfectants decreased the viral titre insufficiently. Under the same conditions, Aujeszky's disease virus was inactivated by at least 4 lg by all the tested disinfectants except for Chloramin BM which decreased the titre of CAPM V-166 only by 3.75 lg in the carrier test. For the inactivation of Vesicular stomatitis virus Chloramin BM and Mikasept KP were tested. Both the disinfectants reliably decreased the viral titre in both the suspension and carrier tests. Our results show that the inactivation of a surface-bound virus is more difficult than its inactivation in suspension. We confirm the high resistance of non-enveloped viruses (Porcine teschovirus) to chemical inactivation. Keywords: virucidal activity, suspension test, carrier test Journal: Veterinární medicína Pages: 236-242 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1949-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1949-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0002.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1949-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.C.M. Couto Author-Workplace-Name: Institute of Veterinary Science, Department of Parasitology, Rural Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Author-Name: S. Quinelato Author-Workplace-Name: Institute of Veterinary Science, Department of Parasitology, Rural Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Author-Name: C.N. Santos Author-Workplace-Name: Institute of Veterinary Science, Department of Parasitology, Rural Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Author-Name: L.S. Souza Author-Workplace-Name: Institute of Veterinary Science, Department of Parasitology, Rural Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Author-Name: I.B.M. Sampaio Author-Workplace-Name: Federal University of Minas Gerais, School of Veterinary Medicine, Department of Animal Science, Belo Horizonte, Minas Gerais, Brazil Author-Name: M.L.A. Rodrigues Author-Workplace-Name: Institute of Veterinary Science, Department of Parasitology, Rural Federal University of Rio de Janeiro, Rio de Janeiro, Brazil Title: Environmental influence in cyathostominae ecology Abstract: Studies of the survival, recovery and migration of cyathostomin infective larvae in a Bermuda grass (Cynodon dactylon) pasture were carried out in the Baixada Fluminense county, Rio de Janeiro state. Fresh feces (± 1 kg) from naturally infected horses were deposited monthly on Bermuda grass. Samples of feces and surrounding grass were collected every seven days, from March 2005 to March 2007, and larva were counted. In the feces, cyathostomin L3 survived for up to 15 weeks, with higher recovery rates during the rainy period (46 228/kg dried herbage - dh), and on the grass for up to 12 weeks. The recovery of L3 was greater during the dry period in the grass base (1 868/kg dh) compared to the apex (809/kg dh). The migration of L3from feces to grass varied during the period. Climatic factors, such as temperature and rain, influenced the development and migratory behavior of cyathostomin L3. With regard to the grass base, significant differences were observed at the different collection times. The results demonstrate that under local conditions animals are at permanent risk since the infective larvae are always present on pasture. Keywords: cyathostomin, Bermuda grass, infective larvae, survival, development Journal: Veterinární medicína Pages: 243-249 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1950-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1950-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0003.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1950-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Kozubska-Sobocinska Author-Workplace-Name: National Research Institute of Animal Production, Balice n. Krakow, Poland Author-Name: B. Rejduch Author-Workplace-Name: National Research Institute of Animal Production, Balice n. Krakow, Poland Author-Workplace-Name: Department of Biotechnology, University of Rzeszow, Kolbuszowa n. Rzeszow, Poland Title: Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism Abstract: The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring. Keywords: sheep, leukocyte chimerism, FISH, spermatozoa, heterosomes Journal: Veterinární medicína Pages: 250-254 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1855-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1855-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0004.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1855-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Pisek Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: J. Travnicek Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: J. Salat Author-Workplace-Name: Institute of Parasitology of the Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic Author-Name: V. Kroupova Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: M. Soch Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Title: Changes in white blood cells in sheep blood during selenium supplementation Abstract: The aim of the experiment was to evaluate the impact of selenium supplementation on white blood cell parameters in the blood of ewes. The total white blood cell (WBC) and differentiation of leukocytes in blood smear were detected by a microscopic analysis, and the CD4+ and CD8+ subsets were detected by flow cytometry. A decrease in the count of WBC was recorded during pregnancy; it was statistically significant only in the group supplemented with organic selenium. In the postpartal period there was a statistically significant increase in the percentages of CD4+ and CD8+ subsets but differences between the groups were not statistically significant. The results of the experiment documented that the supplementation of different forms of selenium did not markedly influence the dynamics of blood parameters in non-pregnant, pregnant and lactating ewes if the intake of vitamins and other essential microelements was adequate. Keywords: ewes, immunity, T lymphocytes, CD4+, CD8+ Journal: Veterinární medicína Pages: 255-259 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1947-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1947-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0005.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1947-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Rodinova Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: V. Kroupova Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: J. Travnicek Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: M. Stankova Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: L. Pisek Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Title: Dynamics of IgG in the blood serum of sheep with different selenium intake Abstract: An experiment was conducted to study the effect of supplementation of ewes with selenium in an inorganic and organic form on the level of IgG during infertility, pregnancy and lactation and in their offspring until two months of age. Control Group C (n = 5) was without Se supplementation. Experimental groups: E1 (n = 5) was supplemented with 180 μg Na2SeO3 per ewe/day, E2 (n = 5) received 180 μ Se per ewe/day organically bound in the biomass of an alga of the genus Chlorella. The average number of newborn lambs per lambing ewe was 1.0, 1.0 and 1.8 in Group C, E1 and E2, respectively. The supplementation did not influence either weight or growth of lambs. The content of IgG increased significantly in ewes in the last third of pregnancy regardless of Se supplementation while their postpartal decrease (P < 0.01) followed only in Group C. Lambs in Group C had a significantly lower level of IgG on Day 30 (P < 0.05) and 60 (P < 0.01) after birth especially in comparison with Group E2. The results document a lower level of immunity in the postpartal period of lambing ewes without Se supplementation and enhancement of postnatal proteosynthesis in the offspring of lambing ewes supplemented with the organic form of Se. Keywords: selenium, Chlorella, Na2SeO3, lambing ewes, lambs Journal: Veterinární medicína Pages: 260-265 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1944-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1944-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0006.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1944-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Halouzka Author-Workplace-Name: Institute of Vertebrate Biology of the Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: Z. Juricova Author-Workplace-Name: Institute of Vertebrate Biology of the Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Name: J. Jankova Author-Workplace-Name: Institute of Vertebrate Biology of the Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Workplace-Name: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic Author-Name: Z. Hubalek Author-Workplace-Name: Institute of Vertebrate Biology of the Academy of Sciences of the Czech Republic, Brno, Czech Republic Author-Workplace-Name: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic Title: Serologic survey of wild boars for mosquito-borne viruses in South Moravia (Czech Republic) Abstract: A serosurvey for mosquito-borne viruses was carried out in 93 wild boars (Sus scrofa), using a plaque-reduction neutralization microtest with Vero cells. The boars were sampled on 24 hunting grounds of the Breclav district (South Moravia) from 2000 to 2002. Specific antibodies to Flavivirus West Nile (WNV) were detected in six (6.5%) animals, and only in Lanzhot and Kostice, i.e., in the area of the "Soutok" game reserve where WNV was previously isolated from mosquitoes in South Moravia. However, the antibody titres were comparatively low (1:20-1:40). A substantially higher seroprevalence was revealed against Orthobunyavirus Tahyna (TAHV): 18 (19.4%) wild boars were positive, and the titres ranged from 1:20 up to 1:640. Only one animal (1.1%) seroreacted with Orthobunyavirus Batai (Calovo), at a low titre of 1:20. The sera were additionally examined by a haemagglutination-inhibition test against Alphavirus Sindbis: two boars (2.2%) revealed antibodies, the titres were 1:20 and 1:80. The serosurvey indicates that the activity of mosquito-borne viruses in South Moravia has decreased compared with the past decades, but that surveillance for these viruses is still necessary. Keywords: antibodies, West Nile virus, Tahyna virus, Batai virus, Sindbis virus, Czechland, swine Journal: Veterinární medicína Pages: 266-271 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1945-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1945-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0007.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1945-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Aydin Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Title: The morphology of circulus arteriosus cerebri in the red squirrel (Sciurus vulgaris) Abstract: In this study, the circulus arteriosus cerebri of the squirrel was investigated. Ten squirrel were used. Coloured latex was given from left ventriculi of the all squirrels. Circulus arteriosus cerebri was examined after the dissection was made. The basilar artery was formed by merge of the right and left vertebral artery. The caudal communicans artery which was caudal part of circulus arteriosus cerebri was formed by the basilar artery on sulcus pontocrurale. From caudal to cranial, the branches originated from the basilar artery and circulus arteriosus cerebri to cerebrum and cerebellum were as follows: the caudal cerebelli artery,rami ad pontem, the rostral cerebelli artery, the caudal choroidea artery, the caudal cerebral artery, the internal ophtalmic artery, the rostral choroidea artery, the media cerebral artery, rami striati and the rostral cerebral artery. In squirrels a variability was observed in the branches that the rostral cerebral artery gives, and their endings. It was determined that the internal carotid artery didn't exist in 4 animals when the right and left vertebral artery were ligatured. It was found that the internal carotid artery didn't contribute to the arterial blood to circulus arteriosus cerebri and the arterial blood to circulus arteriosus cerebri of squirrels is provided via only the basilar artery. Keywords: circulus arteriosus cerebri, brain, red squirrel (Sciurus vulgaris) Journal: Veterinární medicína Pages: 272-276 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1948-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1948-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0008.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1948-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.Y. Heo Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: H.B. Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: K.C Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: N.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Title: Reconstruction of temporomandibular joint ankylosis with temporalis myofascial flap in a cat: a case report Abstract: An approximately 7-year-old 1.7kg, female Siamese cat with a history of a malocclusion and an inability to open its mouth was referred the Animal Medical Center, Chonbuk National University. The cat had shown signs since it was adopted four years earlier but the cause was not determined. The mandible was deviated 2 mm to left, the mouth could be opened only 2 mm and attempts to open the jaws caused considerable pain. Radiographs indicated new bone formation and a loss of joint space in the right temporomandibular joint(TMJ). 3-dimensional CT showed bony fusion and bone thickness in the right TMJ. The physical, 3-dimensional CT and radiographic examinations revealed right TMJ ankylosis. Under general anesthesia, surgical treatment was performed to remove the ankylosis block with subsequent interpositional arthroplasty using a temporalis myofascial flap. The patient showed distinct improvement in both the articular functionality and clinical signs. Keywords: condylectomy, interpositional arthroplasty, ankylotic block Journal: Veterinární medicína Pages: 277-281 Volume: 53 Issue: 5 Year: 2008 DOI: 10.17221/1951-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1951-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200805-0009.txt Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1951-VETMED