Template-Type: ReDIF-Article 1.0 Author-Name: M.K. Kowalik Author-Workplace-Name: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland Author-Name: D. Slonina Author-Workplace-Name: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland Author-Name: J. Kotwica Author-Workplace-Name: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland Title: Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells Abstract: Progesterone (P2 (PGES), (b) protein expression of COX-2, PGFS and PGES, and (c) P4 receptor membrane component 1 (PGRMC1) gene expression in bovine endometrial cells. The epithelial endometrial cells (2.5 × 105/ml) from Days 14-16 of the oestrous cycle were incubated for 72-96 h to attach the cells to the bottom of a well. Next, the cells were preincubated for 30 min with P4 and P5 (10-5M each) and incubated for 4 h and 6 h alone or with OT (10-7M). Thereafter, the medium was collected for PGE2 and PGFM determination, while cells were harvested for gene and protein expression analysis. The used steroids: (a) inhibited OT-stimulated PGF2α, but not PGE2 secretion from endometrial cells, (b) did not affect the expression of mRNA for COX-2, PGFS, PGES and PGRMC1 in endometrial cells after 4 and 6 h, (c) they decreased OT-stimulated COX-2 mRNA expression only after 6 h incubation, and (d) did not influence COX-2, PGFS and PGES protein expression after 6 h. These results indicate that P4 and P5 inhibit OT-stimulated secretion/production of luteolytic PGF2α by a transcription-independent mechanism and partly by down-regulation of COX-2 mRNA. Keywords: non-genomic effect, progesterone, progesterone membrane receptor component 1, endometrium, bovine Journal: Veterinární medicína Pages: 205-214 Volume: 54 Issue: 5 Year: 2009 DOI: 10.17221/58/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/58/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200905-0001.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:5:id:58-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Honsova Author-Workplace-Name: Institute for Clinical and Experimental Medicine, Prague, Czech Republic Author-Name: A. Lodererova Author-Workplace-Name: Institute for Clinical and Experimental Medicine, Prague, Czech Republic Author-Name: P. Balaz Author-Workplace-Name: Institute for Clinical and Experimental Medicine, Prague, Czech Republic Author-Name: M. Oliverius Author-Workplace-Name: Institute for Clinical and Experimental Medicine, Prague, Czech Republic Title: Plasma citrulline levels and acute cellular rejection early after small bowel transplantation in pigs Abstract: Small bowel transplantations (SBT) are increasingly performed to treat patients with irreversible intestinal failure or short-bowel syndrome. Histologic evaluation of small bowel allograft biopsies is important for the diagnosis of acute cellular rejection (ACR). A reliable serological marker of ACR after SBT is still unknown. Recently, citrulline was identified as a potential biomarker of reduced enterocyte mass. The aim of our study was to analyze rejection and plasma citrulline levels early after SBT in pigs. 24 pigs were used and divided into four groups. Group A, autologous SBT (n = 3) as a control group; Group B, allogeneic SBT with tacrolimus monotherapy (n = 7); Group C, allogeneic SBT immunosuppressed with tacrolimus and sirolimus (n = 8); and Group D, without immunosuppresion (n = 6). The observation period was 30 days. Mucosal biopsies were obtained on Days 0, 3, 5, 7, 10, 14, 20, 28 and simultaneously plasma citrulline levels were measured. ACR was classified according to standardized grading schema on a scale of indeterminate, mild, moderate, and severe. There were no significant differences in citrulline plasma levels between cases with mild ACR and indeterminate for ACR. A significant decline in plasma citrulline levels occurred in cases of moderate and severe rejection. Plasma citrulline levels constituted a marker of more advanced injury of small bowel epithelium. Keywords: citrulline, small bowel, transplantation Journal: Veterinární medicína Pages: 215-222 Volume: 54 Issue: 5 Year: 2009 DOI: 10.17221/47/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/47/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200905-0002.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:5:id:47-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Sinkorova Author-Workplace-Name: Faculty of Military Health Sciences, Hradec Kralove, University of Defense, Brno, Czech Republic Author-Name: J. Sinkora Author-Workplace-Name: Becton Dickinson Czechia, Prague, Czech Republic Author-Name: L. Zarybnicka Author-Workplace-Name: Faculty of Military Health Sciences, Hradec Kralove, University of Defense, Brno, Czech Republic Author-Name: Z. Vilasova Author-Workplace-Name: Faculty of Military Health Sciences, Hradec Kralove, University of Defense, Brno, Czech Republic Author-Name: J. Pejchal Author-Workplace-Name: Faculty of Military Health Sciences, Hradec Kralove, University of Defense, Brno, Czech Republic Title: Radiosensitivity of peripheral blood B cells in pigs Abstract: : Swine are here introduced to biodosimetry in an attempt to develop a large animal model allowing for comparison of in vitro experiments with the in vivo processes occurring after exposure to gamma radiation. This work investigates the radiosensitivity of the B cell compartment in peripheral blood. Four-week-old piglets were irradiated using the whole body protocol or full blood samples were irradiated in vitro in the dose range of 0-10 Gy. Relative radioresistance of B cell subpopulations and subsets was determined by measuring their relative numbers in leukocyte preparations at selected time intervals after irradiation using two color immunophenotyping and flow cytometry. Porcine B cells represent the most radiosensitive lymphocyte population in peripheral blood. Among B cell subpopulations and subsets investigated, the CD21+SWC7+ and CD21+CD1+ cells are highly radiosensitive and possess biodosimetric potential, at least in the range of low doses. Differences between cultures irradiated in vitro and lymphocyte dynamics in peripheral blood of irradiated animals clearly document the limits of in vitro data extrapolation in biodosimetry. We have shown that pigs can successfully be used in radiobiology and experimental biodosimetry due mainly to their availability, size and a relatively broad spectrum of available immunoreagents for lymphocyte classification. Keywords: dosimetry-biological, B lymphocytes, radiosensitivity, pig Journal: Veterinární medicína Pages: 223-235 Volume: 54 Issue: 5 Year: 2009 DOI: 10.17221/59/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/59/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200905-0003.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:5:id:59-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Kralova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Leva Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Toman Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Polymorphonuclear function in naturally occurring renal failure in dogs Abstract: Chronic renal failure causes immunosuppression in people and is thought to be one of the causes of non-infectious secondary immunosuppression in dogs. The purpose of this study was to evaluate changes in counts and activity of polymorphonuclears in dogs with chronic renal failure in various stages. Haematological, biochemical examinations and examination of non-specific immune response cells (total and differential leukocyte counts, phagocytosis of methacrylate particles, chemiluminescence test, and level of lysozyme) were performed in blood samples obtained from these dogs. Neutrophilia, lymphopoenia and a decreased number of eosinophils in comparison with healthy control were the main findings in groups with clinical signs. We found the statistically highly significant elevation of lysozyme level; it was in a strong positive correlation with the level of urea, creatinine and phosphorus. We did not find any statistically significant changes in phagocytosis process and other serological factors. In conclusion, despite the reports from human medicine, chronic renal failure in dogs does not alter phagocytosis. From this aspect, the elevation of lysozyme level is the main effect of uraemia. Keywords: leukocytes, uraemia, phagocytosis Journal: Veterinární medicína Pages: 236-243 Volume: 54 Issue: 5 Year: 2009 DOI: 10.17221/11/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/11/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200905-0004.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:5:id:11-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B. Bezdekova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: J. Hanak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Pyloric stenoses in horses: a seven case reports Abstract: The seven cases presented here were diagnosed at the Equine Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic between the years 2002 and 2008. The age range of affected horses was from 10 months to 10 years and included four Thoroughbreds and three warm blooded horses. The horses showed different clinical signs - recurrent or acute colic, poor appetite, frequent recumbency and poor performance. Physical examination revealed diverse results in each horse. Results of gastroscopic examination showed severe nonglandular mucosa ulceration and pathological changes along the pylorus in all horses. Two horses showed distal esophagitis. The duodenum was visible and endoscopically unchanged in two horses. Treatment was applied to four horses with different results. Follow up: Two horses are alive and they have partially or fully returned to their previous performance level. Five horses were euthanised because of clinical worsening and/or unsuccessful treatment. Four horses were examined post mortem. The final diagnosis of pyloric stenosis was made by gastroscopy in six horses. In the seventh horse it was confirmed by necropsy. Gastric or gastroduodenal ulceration was revealed as the cause of pyloric stenosis in five horses. In two horses the origins of pyloric changes were uncertain. Keywords: horse, pyloric stenosis, gastric ulceration, colic Journal: Veterinární medicína Pages: 244-248 Volume: 54 Issue: 5 Year: 2009 DOI: 10.17221/1/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200905-0005.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:5:id:1-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: B. Bezdekova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Faldyna Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: O. Zapletal Author-Workplace-Name: University Hospital, Brno, Czech Republic Author-Name: P. Jahn Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Acute B-lymphoid leukemia in a mare: a case report Abstract: An 8-year-old Friesen mare was admitted to the Equine Clinic, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic with ventral edema and weight loss which had proceeded over the course of a few weeks. Clinical examination revealed poor body condition, depression, weakness, pale mucous membranes, enlarged mandibular lymph nodes and ventral edema. Thoracic percussion revealed fluid level reaching the shoulder on both sides. CBC revealed a severe disproportion of red and white cells. The horse was anemic and there was leucocytosis with the majority being blast cells (leucocyte concentration was 450.4 × 109/l) which included mitotic figures. The serum chemistry profile revealed hypoalbuminemia and increased alkaline phosphatase bone isoenzyme (BALP was 784.3 μkat/l). Based on the number of precursors in the peripheral blood acute leukemia was diagnosed. The peripheral blood was examined by flow cytometry and cytochemistry. The final diagnosis was determined as acute B cell lymphoid leukemia. The mare was treated with corticosteroids. On the second day of treatment the white blood cell count and alkaline phosphatase level decreased. The improvement continued until the fifth day, when the mare's status deteriorated and the horse was euthanized. Gross examination was carried out but no neoplastic mass was discovered. Final differentiation between primary and secondary lymphoid leukemia was not possible in this case. Keywords: horse, leukemia, flow cytometry, cytochemistry, lymphoma Journal: Veterinární medicína Pages: 249-255 Volume: 54 Issue: 5 Year: 2009 DOI: 10.17221/2924-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2924-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200905-0006.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:5:id:2924-VETMED