Template-Type: ReDIF-Article 1.0 Author-Name: L. Hasonova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Workplace-Name: Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic Author-Name: I. Trcka Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Babak Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: Z. Rozsypalova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: R. Pribylova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Distribution of Mycobacterium avium subsp.paratuberculosis in tissues of naturally infected cattle as affected by age Abstract: ABSTRACT: The objectives of this study were to investigate Mycobacterium avium subsp. paratuberculosis (MAP) distribution in various tissues, in faeces and in milk samples from 131 animals originating from one imported Jersey cattle herd from Denmark. MAP was detected by culture in 37.4% animals. Massive MAP growth was most often observed in the small intestines (48 animals). The lowest levels of MAP were found in spleen and mammary gland samples. MAP was detected in the faeces of 8.4% animals; however, milk samples were MAP negative by culture. The highest prevalence of MAP infection (42.9%) was in age Group B (1.6 to 3 years) and the lowest (6.1%) in group D (more than 8 years). Seven positive calves were detected in the youngest age group; one of them was less than one month of age, which could imply an intrauterine infection. MAP shedding in faeces by a five-month-old calf was also confirmed. The IS900 RFLP (restriction fragment length polymorphism) type B-C1 was identified in all animals. Keywords: Johne's disease, food safety, age groups Journal: Veterinární medicína Pages: 257-269 Volume: 54 Issue: 6 Year: 2009 DOI: 10.17221/54/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/54/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200906-0001.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:6:id:54-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Pate Author-Workplace-Name: Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: M. Moravkova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: B. Krt Author-Workplace-Name: Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Ocepek Author-Workplace-Name: Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Title: Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP Abstract: ABSTRACT: Apart from birds, Mycobacterium avium subsp. avium (MAA) is often isolated from granulomatous lesions in pigs and occasionally from cattle and other animals. The objectives of this study were the detection of IS901 restriction fragment length polymorphism (RFLP) types of MAA isolates from different species of domestic animals between the years 1998 and 2004 and the comparison of the detected RFLP types with previously described RFLP types collected in the database of the OIE Reference Laboratory for Avian Tuberculosis (Brno, Czech Republic). Furthermore, the RFLP types of the isolates obtained from MAA outbreaks on one of the largest pig farms in Slovenia were also investigated. A total of 62 isolates (56 from pigs, five from poultry and one from cattle) were identified with IS901 PCR and IS901 RFLP typed using restriction endonucleases PvuII and PstI. Seven PvuII RFLP and 11 PstI RFLP types resulted in 12 combined PvuII PstI types; none of these matched the combined RFLP types described in previous studies. Our contributions to the database were two new PvuII and eight new PstI RFLP types. Identical RFLP types were found among isolates of animals originating from individual farms. Finding of identical RFLP types within a farm is not surprising because the animals were epidemiologically related and infected with one strain. A unique RFLP type F-A17 was detected in isolates from different pig herds and also in isolates from poultry. Detection of identical RFLP types on different farms may reflect one MAA source. The other combined PvuII PstI RFLP types were identified only once which indicates considerable variety of MAA RFLP types in Slovenia. Keywords: genotyping, mycobacteriosis, avian tuberculosis, molecular epidemiology, zoonosis, database for IS901 RFLP types Journal: Veterinární medicína Pages: 270-279 Volume: 54 Issue: 6 Year: 2009 DOI: 10.17221/3084-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/3084-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200906-0002.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:6:id:3084-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: C. Hu Author-Workplace-Name: Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Author-Name: Y. Ding Author-Workplace-Name: Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Author-Name: J. Chen Author-Workplace-Name: Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Author-Name: D. Liu Author-Workplace-Name: Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Author-Name: M. Ding Author-Workplace-Name: Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Author-Name: Y. Zhang Author-Workplace-Name: Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China Title: Treatment of corneal epithelial wounds in dogs using basic fibroblast growth factor Abstract: An experimental study examined the effect of basic fibroblast growth factor (bFGF) on the healing of corneal epithelial wounds in dogs. A corneal wound was made on one eye from each of 40 dogs with a corneal trephine (6 mm diameter). Four concentrations of bFGF (0, 0.1, 0.5, and 1.0 μg/ml) were applied to the affected eyes three times daily. Fluorescein staining was used to assess the closure of the corneal epithelial wounds. The morphological characteristics were determined on histological examination. The wound healing rate was significantly greater in the bFGF-treated group compared with controls 1, 3, 5, and 7 days (P < 0.01) after the topical administration of bFGF. Both 0.5 and 1.0 μg/ml bFGF increased the wound healing rate significantly (at Days 3 and 5, P < 0.05) compared to 0.1 μg/ml bFGF. Moreover, two control cases still showed poor healing 10 days after the corneal wound. None of the eyes developed corneal clouding or neovascularization during the experiment. The histological examination showed more epithelial layers, a more regular rearrangement, and fewer inflammatory cells in the epithelium of the bFGF-treated group; the epithelium was reconstructed more quickly in the bFGF-treated group compared with the control group. These results suggest that bFGF promotes canine corneal epithelial wound healing effectively, making bFGF suitable for curing canine corneal epithelial wounds. Keywords: corneal epithelium, corneal epithelial wound healing, basic fibroblast growth factor, bFGF, dogs Journal: Veterinární medicína Pages: 280-286 Volume: 54 Issue: 6 Year: 2009 DOI: 10.17221/93/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/93/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200906-0003.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:6:id:93-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Serin Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: U. Parin Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Title: Recurrent vaginal discharge causing by retained foetal bones in a bitch: a case report Abstract: An 18-month-year old mixed breed bitch was referred to the Animal Hospital of Adnan Menderes University suffering from recurrent serous vaginal discharge for two months. Her temperature, pulsation, appetite and also hematologic parameters were normal. Transabdominal ultrasound scan revealed fullness in bifurcation uteri and bright echoes suggestive of bone caudally. During hysterotomy, retained foetal bones were observed in corpus uteri but not removed as they had become embedded in the uterine wall. Therapy of this case was completed with ovariohysterectomy operation. No aerobic bacteria were isolated from both samples taken from the vaginal discharge and retained foetal bones in the uterine cavity. Although rare, veterinary clinicians should be aware of this problem also in young bitches with no general symptoms and are well advised to use genital ultrasonography in examinations. Keywords: foetal death, bitch, ultrasonography Journal: Veterinární medicína Pages: 287-290 Volume: 54 Issue: 6 Year: 2009 DOI: 10.17221/90/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/90/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200906-0004.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:6:id:90-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Smolec Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zgreb, Croatia Author-Name: J. Kos Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zgreb, Croatia Author-Name: D. Vnuk Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zgreb, Croatia Author-Name: T. Babic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zgreb, Croatia Author-Name: N.B. Bottegaro Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zgreb, Croatia Title: Abdominal ventral hernia in a pigeon (Columba livia): a case report Abstract: Reports of abdominal hernias in birds are often not clearly described. Abdominal hernias have been characterized as a separation in the aponeurosis of the abdominal musculature on the ventral midline. In this article one case of abdominal hernia is described. A painless, reducibile swelling was located in the ventral abdominal region close to the cloaca. Surgical repair was performed because the bird was stable clinically and an hernioraphy was indicated. A ventral midline celiotomy was performed. For repair of the abdominal hernia the abdominal muscles and skin were sutured in a standard two-layer closure using a simple continuous suture pattern. This procedure is safe and uncomplicated and this approach can be recommended as a curative method for abdominal hernia repair. Keywords: pigeon, hernia, repair, adhesion Journal: Veterinární medicína Pages: 291-294 Volume: 54 Issue: 6 Year: 2009 DOI: 10.17221/89/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/89/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200906-0005.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:6:id:89-2009-VETMED