Template-Type: ReDIF-Article 1.0 Author-Name: B. Robesova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Kovarcik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: S. Vilcek Author-Workplace-Name: University of Veterinary Medicine, Kosice, Slovakia Title: Genotyping of bovine viral diarrhoea virus isolates from the Czech Republic Abstract: This study was focused on the genetic typing of bovine viral diarrhoea virus (BVDV) isolates obtained from 41 serum samples of persistently infected cattle in the Czech Republic in the period of 2004 to 2007. For the differentiation of BVDV isolates, the 5'-UTR and Npro regions were selected. A 288-bp fragment from 5'-UTR and 428-bp fragment from Npro of the selected isolates were amplified by RT-PCR and subsequently sequenced and analysed by computer-assisted phylogenetic analysis. The isolates belong to BVDV-1 genotype and the following subtypes were identified: b (n = 16), d (n = 16), e (n = 2) and f (n = 7). In this collection of viral samples, no isolate belonged to BVDV-2 genotype. Keywords: BVDV, phylogenetic analysis, genotyping, cattle Journal: Veterinární medicína Pages: 393-398 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/3053-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/3053-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0001.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:3053-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A.M. Abdelaal Author-Workplace-Name: Faculty of Veterinary Medicine, Zagazig University, Egypt Author-Name: M. Floeck Author-Workplace-Name: Clinic for Ruminants, University of Veterinary Medicine, Vienna, Austria Author-Name: S. El Maghawry Author-Workplace-Name: Faculty of Veterinary Medicine, Zagazig University, Egypt Author-Name: W. Baumgartner Author-Workplace-Name: Clinic for Ruminants, University of Veterinary Medicine, Vienna, Austria Title: Clinical and ultrasonographic differences between cattle and buffaloes with various sequelae of traumatic reticuloperitonitis Abstract: This study was performed to document the clinical and ultrasonographic differences between cattle and buffaloes with various sequelae of traumatic reticuloperitonitis (TRP) and the importance of ultrasonography in detection of such sequelae. Twenty nine cows and 33 buffaloes with TRP were investigated. By using ultrasonography and some confirmatory techniques as paracentesis, laparotomy and necropsy, the various sequelae of TRP in both species were clarified. Acute local peritonitis, chronic local peritonitis, acute diffuse peritonitis, reticular abscesses, thoracic abscesses and pericarditis have been detected as sequelae of TRP in both cattle and buffaloes. The clinical findings of all sequelae are described. The results of the present study indicate that the classical symptom of pain and systemic reactions were common in most sequelae of TRP in cattle and less common in buffaloes. No specific findings have been recorded for reticular abscesses in both species. Brisket oedema and distended jugular veins have been shown in both cattle and buffaloes with pericarditis and also in four buffaloes with thoracic abscesses. Hence, it is important to use ultrasonography for early detection of TRP especially in buffaloes and to discriminate between different sequelae which have the same clinical findings. Keywords: buffaloes, cattle, traumatic reticuloperitonitis, sonography, clinical findings Journal: Veterinární medicína Pages: 399-406 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/128/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/128/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0002.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:128-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: U. Polat Author-Workplace-Name: Faculty of Veterinary Medicine, University of Uludag, Turkey Author-Name: H. Gencoglu Author-Workplace-Name: Faculty of Veterinary Medicine, University of Uludag, Turkey Author-Name: I.I. Turkmen Author-Workplace-Name: Faculty of Veterinary Medicine, University of Uludag, Turkey Title: The effects of partial replacement of corn silage on biochemical blood parameters in lactating primiparous dairy cows Abstract: The aim of this experiment was to investigate the effects of partial replacement of corn silage with long alfalfa hay and/or coarse chopped wheat straw on biochemical parameters in late lactating Holstein primiparous dairy cows. Twelve late lactating Holstein primiparous cows including four cows equipped with a rumen cannula, averaging 210 ± 20 days in milk and weighing 575 ± 50 kg were randomly assigned in a 4 × 4 Latin square design. During each of four 21-days periods, cows were fed four total mixed diets that were varied in the forage sources: (1) 50% corn silage (CS), (2) 35% corn silage + 15% wheat straw (CSW), (3) 35% corn silage + 15% alfalfa hay (CSA), (4) 25% corn silage + 10% wheat straw + 15% alfalfa hay (CSWA). At the end of the feeding period, blood samples were taken from jugular vein. Serum glucose, total protein, albumin/globulin, creatinine, creatinin kinase, urea and uric acid levels were determined by using Auto Technicon DAX 72 autoanalyzer. While serum glucose, albumin, globulin, urea, uric acid and creatinine kinase activity were not found significantly different among groups, serum total protein (between CS and CSA, CS and CSWA) and creatinine (between CSA and CSWA) levels had statistical significance at the levels of P < 0.05. It is concluded that rumen propionate and ammonia levels may be good indicator for glucose and BUN, respectively. Keywords: forage sources, corn silage, blood, cows Journal: Veterinární medicína Pages: 407-411 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/72/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/72/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0003.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:72-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Mazur Author-Workplace-Name: Institut National de la Recherche Agronomique (INRA), Clermont-Ferrand/Theix, St Genes Champanelle, France Author-Name: M. Ozgo Author-Workplace-Name: Institut National de la Recherche Agronomique (INRA), Clermont-Ferrand/Theix, St Genes Champanelle, France Author-Workplace-Name: Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland Author-Name: Y. Rayssiguier Author-Workplace-Name: Institut National de la Recherche Agronomique (INRA), Clermont-Ferrand/Theix, St Genes Champanelle, France Title: Altered plasma triglyceride-rich lipoproteins and triglyceride secretion in feed-restricted pregnant ewes Abstract: In late pregnancy, energy deficits in ewes can induce a metabolic imbalance, which often results in pregnancy toxaemia. This metabolic disorder is characterised by hypoglycaemia, hyperketonaemia, an increase in plasma concentrations of free fatty acids and by fatty infiltration in the liver. The purpose of the present study was to identify alterations in lipid and triglyceride-rich lipoprotein distribution and to evaluate triglyceride secretion in ewes during the third trimester of pregnancy, particularly in ewes with feed restriction. The study was performed on non-pregnant and on twin- and triplet-bearing pregnant Limousine × Romanov ewes fed a control or restricted diet. We show that in pregnant ewes, feed restriction resulted in strong lipomobilisation, as monitored by high plasma free fatty acid concentration, and in hypoglycaemia and hyperketonaemia. Plasma and very low density lipoprotein (VLDL) triglyceride concentrations were about four-fold higher in adequately-fed pregnant ewes than in non-pregnant ewes. Feed restriction in pregnant ewes resulted in VLDL triglyceride concentrations that were approximately two-thirds lower than in adequately-fed pregnant ewes. VLDL particles from pregnant ewes were found to be enriched in triglycerides, but to a lesser extent in feed-restricted pregnant ewes. Pregnant ewes that were fed an adequate diet exhibited greater triglyceride secretion rates (TGSR) than non-pregnant ewes. Feed restriction in pregnant ewes resulted in triglyceride secretion rates that were approximately 35% lower than those of pregnant ewes fed an adequate diet. Our results support the idea that exacerbated lipomobilisation in late pregnancy leads to profound lipid and lipoprotein metabolism disturbances. Keywords: lipids, lipoproteins, triglycerides, pregnancy, sheep Journal: Veterinární medicína Pages: 412-418 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/77/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/77/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0004.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:77-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Stastkova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: S. Karpiskova Author-Workplace-Name: Czech Collection of Microorganisms, Institute of Experimental Biology, Masaryk University, Brno, Czech Republic Author-Name: R. Karpiskova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Workplace-Name: National Institute of Public Health, Prague, Brno, Czech Republic Title: Occurrence of methicillin-resistant strains of Staphylococcus aureus at a goat breeding farm Abstract: The aim of this study was to report the detection of methicillin-resistant Staphylococcus aureus (MRSA) strains at a veterinary university goat breeding farm and their characteristics. A total of 278 samples collected from animals, milk, environment and farm personnel between June 2006 and March 2008 were examined. The identification of S. aureus isolates was performed by a species specific PCR assay. All detected isolates were tested for resistance to oxacillin and other antimicrobials by phenotypic methods and for the mecA gene by PCR method. Eight MRSA were detected in this study. Five of them originated from goat's milk and three were recovered from one human carrier of the farm personnel. All obtained MRSA isolates were clonally consistent and were characterized as: SCCmec type IV, spa type t064, seb positive and for genes encoding TSST-1, PVL and exfoliative toxins A and B negative. Keywords: MRSA, PCR, mecA, Panton-Valentine leukocidin, toxic-shock syndrome toxin-1, staphylococcal enterotoxins, exfoliative toxins, SCCmec, PFGE, spa type Journal: Veterinární medicína Pages: 419-426 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/88/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/88/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0005.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:88-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Jarosova Author-Workplace-Name: Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: J. Harazim Author-Workplace-Name: Central Institute for Supervising and Testing, Brno, Czech Republic Author-Name: P. Suchy Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Kratka Author-Workplace-Name: Mendel University of Agriculture and Forestry, Brno, Czech Republic Author-Name: V. Stancova Author-Workplace-Name: Mendel University of Agriculture and Forestry, Brno, Czech Republic Title: The distribution and accumulation of phthalates in the organs and tissues of chicks after the administration of feedstuffs with different phthalate concentrations Abstract: For the monitoring of distribution and accumulation of phthalic acid esters (PAE) in animal tissues, samples of muscle, mesenteric fat (fat), skin and liver from broiler chicks ROSS 308 were used. The chicks were divided into four groups (50 chicks each). All the chicks were given commercial diets (complete feed, KKS) for broiler chicks (starter - BR1; grower - BR2 and finisher - BR3). The experimental diets were supplemented with vegetable oil (RV) with low (group N) or high (group V) phthalate contents, or animal fat with a high phthalate content (group Z). Neither the control diets (K), nor the grower (BR1) diets contained vegetable oils or animal fat. The N chicks were given the grower (BR2) and finisher (BR3) diets supplemented with 5% and 3% vegetable oil, respectively. The V chicks were given BR2 and BR3 diets with 5% and 3% vegetable oil, respectively. The Z chicks were given BR2 and BR3 diets with 5% and 3% animal fat, respectively. The chicks were fattened till 42 days of age. Dibutyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) were found in the tissues of chicks in all the experimental groups. The DBP content in the muscle ranged from 0.03 to 0.55 mg/kg, in the adipose tissue from < 0.20 to 2.56 mg/kg, in the skin from < 0.20 to 1.49 mg/kg, and in the liver from 0.03 to 0.13 mg/kg. The content of DEHP in the muscle ranged from 0.03 to 1.15 mg/kg, in the adipose tissue from 0.25 to 9.85 mg/kg, in the skin from < 0.20 to 4.68 mg/kg, and in the liver from 0.16 to 0.24 mg/kg. The highest concentrations of DBP of 1.28 ± 1.00 mg/kg of fresh sample (an average value from eight chicks) was determined in the adipose tissue of V chicks. The highest concentration of DEHP of 3.27 ± 2.87 mg/kg of fresh sample (mean of eight chicks) was also determined in the V group. The accumulation of DEHP was 3.2; 2.6 and 2.9 times higher than that of DBP in the muscle, adipose tissue and skin, respectively. The V and Z chicks showed higher phthalate contents (the sum of DBP and DEHP) in the adipose tissue, skin and liver than the K and N chicks. Keywords: DBP, DEHP, analysis, monitoring, contamination, HACCP, feed material Journal: Veterinární medicína Pages: 427-434 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/2/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0006.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:2-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: K. Rosenbergova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science Brno, Czech Republic Author-Name: P. Lany Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science Brno, Czech Republic Author-Name: Z. Pospisil Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science Brno, Czech Republic Author-Name: O. Kubicek Author-Workplace-Name: National Institute for Nuclear, Chemical and Biological Protection, Kamenna, Czech Republic Author-Name: V. Celer Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science Brno, Czech Republic Author-Name: D. Molinkova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Science Brno, Czech Republic Title: Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR Abstract: This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic. The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe was developed for a rapid detection and quantification of avian influenza virus RNA in clinical samples collected from mute swans. Conserved regions in the matrix protein gene of avian influenza virus served as targets for the primers and TaqMan probe design. A recombinant plasmid containing the matrix protein gene amplicon was constructed for a quantitative assay of copy numbers of the target gene. Quantification of avian influenza virus RNA was accomplished using a standard curve generated from ten-fold serial dilutions of recombinant plasmid DNA in the range of 102 to 108 copies/µl. Avian influenza virus A/Cygnus olor/Brno-cz/2006 was adapted to grow in VERO cells. In the same passage of cell cultivation, the concentration of viral RNA was determined to be 1.01 × 107 copies/ml and TCID50 was 104.2/ml. From these values the ratio of one RNA copy to 0.00157 virion capable of VERO cells infection was calculated. This ratio was used to estimate the virus concentrations in the tissues of dead mute swans. Keywords: avian influenza virus, qRT-PCR, TagMan probe, TCID50, mute swan Journal: Veterinární medicína Pages: 435-443 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/16/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/16/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0007.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:16-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Laus Author-Workplace-Name: Department of Veterinary Science, University of Camerino, Matelica, Italy Author-Name: A.R. Attili Author-Workplace-Name: Department of Veterinary Science, University of Camerino, Matelica, Italy Author-Name: M. Cerquetella Author-Workplace-Name: Department of Veterinary Science, University of Camerino, Matelica, Italy Author-Name: A. Spaterna Author-Workplace-Name: Department of Veterinary Science, University of Camerino, Matelica, Italy Author-Name: B. Tesei Author-Workplace-Name: Department of Veterinary Science, University of Camerino, Matelica, Italy Author-Name: V. Cuteri Author-Workplace-Name: Department of Veterinary Science, University of Camerino, Matelica, Italy Title: Endoscopic findings, microbiological and cytological evaluation of tracheal aspirates in a population of Standardbred horses with poor performances Abstract: Sixty-two Standardbred horses housed at the same racetrack, with history of reduced exercise tolerance, cough lasting for at least two weeks and/or prolonged recovery time were clinically examined. An endoscopic examination of the nasopharynx, larynx and trachea to the level of the carina was performed, amount of mucus in trachea was registered and samples of tracheal wash for cytological and microbiological examinations was collected. A strong statistical association between amount of mucus in trachea and neutrophils percentage in tracheal wash was found. Bacteria isolated included Streptococcus equi subsp. zooepidemicus (14 horses), Streptococcus pneumoniae (four horses), S. mutans (four horses), S. equinus (four horses) and Burkholderia cepacia (10 horses). S. zooepidemicus and S. pneumoniae were associated with elevated amount of mucus and increased neutrophilic percentage. B. cepacia was associated with cytological evidence of haemosiderophages but its role in racehorses needs further investigations. Mycoplasma spp. and Pasteurella spp. have not been isolated, suggesting that, as for the other putative causes of inflammatory airway diseases, infection could have a regional distribution among horse populations. This study shows that various types of airway inflammations exist in the examined population and that S. zooepidemicus and S. pneumoniae could play an important role in etiopathogenesis of airway inflammation in some horses. Particulate matter, pro-inflammatory agents or noxious gases present in the stables or on the track matter, could be the cause of inflammation in non infected horses. Keywords: horse, tracheal wash, mucus, cytology, bacteriology, Streptococci Journal: Veterinární medicína Pages: 444-450 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/3/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/3/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0008.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:3-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.I. Bhaiyat Author-Workplace-Name: School of Veterinary Medicine, St. George's University, Grenada, West Indies Author-Name: H. Hariharan Author-Workplace-Name: School of Veterinary Medicine, St. George's University, Grenada, West Indies Author-Name: A. Chikweto Author-Workplace-Name: School of Veterinary Medicine, St. George's University, Grenada, West Indies Author-Name: E. Brathwaite-Sylvester Author-Workplace-Name: School of Veterinary Medicine, St. George's University, Grenada, West Indies Author-Name: P.J.A. Burnett Author-Workplace-Name: School of Veterinary Medicine, St. George's University, Grenada, West Indies Author-Name: V. Matthew Author-Workplace-Name: School of Veterinary Medicine, St. George's University, Grenada, West Indies Author-Name: S. Oliveira Author-Workplace-Name: College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA Author-Name: C. Johnson Author-Workplace-Name: College of Veterinary Medicine, Auburn University, AL, USA Title: Concurrent lymphosarcoma and Salmonella enteritidis infection in a cat: a case report Abstract: Concurrent lymphosarcoma and salmonellosis in a 12-year-old female neutered domestic shorthair cat in Grenada is described. Clinically, the cat was emaciated, and had a history of vomiting and diarrhea of two months duration. Clinical examination revealed a large palpable mass in the craniodorsal abdomen and fluid in the thoracic cavity. Gross lesions consisted of moderate pyothorax and pyoabdomen with variably-sized, single to multiple, soft, white masses in the mesentery adjacent to the pancreas, on the serosal surface of the stomach, and on the quadrate lobe of the liver. Histopathological findings associated with these masses were compatible with lymphosarcoma. Sheets of neoplastic round cells, some with intracytoplasmic eosinophilic granules, were found in the masses associated with the omentum, stomach, and liver. Salmonella enteritidis was isolated in pure culture from the thoracic fluid, collected during clinical examination, and mesenteric mass, collected during necropsy, and both isolates showed similar antimicrobial susceptibility patterns. Keywords: cat, lymphosarcoma, salmonella Journal: Veterinární medicína Pages: 451-454 Volume: 54 Issue: 9 Year: 2009 DOI: 10.17221/64/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/64/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-200909-0009.txt Handle: RePEc:caa:jnlvet:v:54:y:2009:i:9:id:64-2009-VETMED