Template-Type: ReDIF-Article 1.0 Author-Name: M. Lopatarova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: S. Cech Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Krontorad Author-Workplace-Name: BOVET Company, Sloupnice, Czech Republic Author-Name: L. Holy Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: H. Lalova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Dolezel Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Conception rate after sex determination and cryopreservation of D7 bovine embryos: Abstract: The aim of this study was to evaluate conception rate after sex determination and subsequent freezing of Day 7 (D7) bovine embryos and to compare it with transfer of fresh female embryos. High quality embryos obtained from superovulated donors were biopsied and the embryonic cells (5-10) were analysed by PCR using specific primers for the Y-chromosome determinant. Fresh embryos were blade biopsied (n = 172) and subsequently transferred (ET) ipsilaterally to synchronized recipients. Selected embryos (n= 112) were biopsied by cell aspiration and cryopreserved for later transfer. Sex determination was successfully completed in 91.3% of fresh group embryos (44% female) and in 87.5% of freeze-thawed group embryos (45.9% female). The achieved pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. After transfer of freeze-thawed sexed (female) embryos, pregnancy was established in 48.8% of recipients. A similar conception rate was found in intact embryos (50.7%, control 2). The results clearly demonstrate that the microsurgical technique used and subsequent PCR sex analysis allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in embryo transfer programs. Keywords: cattle, superovulation, sexing, polymerase chain reaction, cryopreservation, embryo transfer Journal: Veterinární medicína Pages: 10-18 Volume: 55 Issue: 1 Year: 2010 DOI: 10.17221/160/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/160/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201001-0001.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:1:id:160-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.Y. Manga-Gonzalez Author-Workplace-Name: Animal Health Department, Institute of Mountain Livestock (CSIC-ULE), Spanish National Research Council (CSIC), Grulleros, Leon, Spain Author-Name: H. Quiroz-Romero Author-Workplace-Name: Faculty of Veterinary Medicine and Zootechnia, Autonomous National University of Mexico (UNAM), University City, Mexico, D.F. Author-Name: C. Gonzalez-Lanza Author-Workplace-Name: Animal Health Department, Institute of Mountain Livestock (CSIC-ULE), Spanish National Research Council (CSIC), Grulleros, Leon, Spain Author-Name: B. Minambres Author-Workplace-Name: Animal Health Department, Institute of Mountain Livestock (CSIC-ULE), Spanish National Research Council (CSIC), Grulleros, Leon, Spain Author-Name: P. Ochoa Author-Workplace-Name: Faculty of Veterinary Medicine and Zootechnia, Autonomous National University of Mexico (UNAM), University City, Mexico, D.F. Title: Strategic control of Dicrocoelium dendriticum (Digenea) egg excretion by naturally infected sheep Abstract: The aim of this study was to determine the most appropriate months for applying albendazole (ABZ; oral suspension dose 20 mg/kg body weight) to sheep naturally infected with Dicrocoelium dendriticum and kept at pasture, in order to reduce parasite egg shedding to a minimum, mainly during the cold months and, as a result, decrease pasture contamination by viable eggs. Five animal groups (G), homogeneous as regards the number of eggs per gram (EPG) in faeces, were established. The treatment months were: G1, November and January; G2, November and February; G3, November and April; G4, January; and G5 (control), April. Ten samplings (S1-S10) were carried out every 35 to 45 days to collect faecal samples from the rectum of each animal in the five groups. The sedimentation technique and McMaster egg counting chambers were used to analyze the faecal samples. Due to the effect of albendazole (ABZ) treatments, the five groups behaved differently with regard to EPG reduction and the percentage of samples positive for D. dendriticum eggs. Using the Kruskal-Wallis test, statistically significant differences (P < 0.05) were observed between the EPG values obtained in G5 and the rest of the groups from November to May, but not from May onwards. The biggest reduction in egg excretion was obtained in G1, mainly in the cold period when elimination is highest and egg survival greatest, so G1 gave the best result, followed by G2, G4, G3 and finally G5 in descending order. Keywords: dicroceliosis, trematoda, strategic treatment, albendazole, ovine, Spain Journal: Veterinární medicína Pages: 19-29 Volume: 55 Issue: 1 Year: 2010 DOI: 10.17221/63/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/63/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201001-0002.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:1:id:63-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Duras Gomercic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: T. Trbojevic Vukicevic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: T. Gomercic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: A. Galov Author-Workplace-Name: Faculty of Science, University of Zagreb, Croatia Author-Name: T. Fruk Author-Workplace-Name: Veterinary Station of Varazdin, Croatia Author-Name: H. Gomercic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Title: The cisterna chyli and thoracic duct in pigs (Sus scrofa domestica) Abstract: Anatomical variations of the thoracic duct course are common in humans and domestic animals. They are important in thoracic surgery and in application of surgical techniques in experimental animals. The pig is a frequently used animal model due to numerous similarities between human and porcine anatomy and physiology. We revealed the position of the cisterna chyli, and the origin, course and termination of the thoracic duct by fine dissection on fifteen Yorkshire pig carcasses. The pigs were 2.5 months old with a body mass range from 10 to 15 kg. In this study we present our macroscopic observations. The cisterna chyli and thoracic duct had a common position, form and course in ten (67%) specimens. Anatomical variations of the precardiac course of the thoracic duct were observed in five animals (33%). Knowledge of these anatomical features should enhance the use of swine as an experimental model. Keywords: anatomy, lymphatic system, swine Journal: Veterinární medicína Pages: 30-34 Volume: 55 Issue: 1 Year: 2010 DOI: 10.17221/21/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/21/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201001-0003.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:1:id:21-2010-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Sfacteria Author-Workplace-Name: Faculty of Veterinary Medicine, University of Messina, Messina, Italy Author-Name: F. Macrì Author-Workplace-Name: Faculty of Veterinary Medicine, University of Messina, Messina, Italy Author-Name: L. Perillo Author-Workplace-Name: Faculty of Veterinary Medicine, University of Messina, Messina, Italy Author-Name: G. Rapisarda Author-Workplace-Name: Faculty of Veterinary Medicine, University of Messina, Messina, Italy Author-Name: G. Lanteri Author-Workplace-Name: Faculty of Veterinary Medicine, University of Messina, Messina, Italy Author-Name: G. Mazzullo Author-Workplace-Name: Faculty of Veterinary Medicine, University of Messina, Messina, Italy Title: Cytologic and histologic features of spinal cord ependymoma in a young dog: a case report Abstract: A case of intramedullary ependymoma in a young dog is reported. A two year old dog was presented with paralysis of the forelimbs. At myelographic examination, an intramedullary pattern, blocking the progression of contrast medium, was observed. At necropsy, a 3 × 2 cm white-greyish mass was found extending from the 3rd to 5th lumbar levels. At cytological and histological examination, the mass was highly cellular and was comprised of ovoid cells with indistinct borders, elongated eosinophilic cytoplasm and round to oval vesicular nuclei. Cytoplasmic processes formed a fibrillar network wherein true rosettes and many pseudorosettes around a fibrovascular stroma were observed. Immunohistochemistry for vimentin and GFAP gave strong positive results in the neoplastic cells, especially around pseudorosettes and confirmed the diagnosis of intramedullary spinal ependymoma. Keywords: ependymoma, spinal cord, tumor, immunohistochemistry, dog Journal: Veterinární medicína Pages: 35-38 Volume: 55 Issue: 1 Year: 2010 DOI: 10.17221/86/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/86/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201001-0004.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:1:id:86-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Pomianowski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: Z. Adamiak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Title: Bone-conducted brainstem auditory evoked response in a dog with total bilateral ear canal ablation: a case report Abstract: The bone-conducted brainstem auditory evoked response test was performed in an eight-year-old dog with total bilateral ear canal ablation. It was found that 12 months after bilateral surgical ablation of the external acoustic meatus the dog still maintained its hearing function due to the mechanism of acoustic bone conduction. Keywords: bone conduction, hearing, dog Journal: Veterinární medicína Pages: 39-41 Volume: 55 Issue: 1 Year: 2010 DOI: 10.17221/20/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/20/2010-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:55:y:2010:i:1:id:20-2010-VETMED