Template-Type: ReDIF-Article 1.0 Author-Name: A. Brtkova Author-Workplace-Name: State Veterinary and Food Institute, Dolny Kubin, Slovak Republic Author-Name: M. Filipova Author-Workplace-Name: State Veterinary and Food Institute, Dolny Kubin, Slovak Republic Author-Name: H. Drahovska Author-Workplace-Name: Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Author-Name: H. Bujdakova Author-Workplace-Name: Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic Title: Characterization of enterococci of animal and environmental origin using phenotypic methods and comparison with PCR based methods Abstract: The purpose of this study was to evaluate the discriminatory power of ddl-PCR (D-alanin-D-alanin ligase PCR) and ITS-PCR (Internal Transcribed Spacer PCR) for accurate identification of enterococcal species in comparison with phenotypic assays. Results confirm previous published data that ddl-PCR simple approach allows rapid identification of two the most frequently isolated spp. E. faecalis and E. faecium. When identification points towards other enterococci then that mentioned above, the ITS-PCR seems to be suitable complementary assay. Correct identification of enterococci to species level is important because of different spp. susceptibility to some clinically important antibiotics. For example, it is necessary to distinquish acquired vancomycin resistance from inherent one that is less epidemiologically important. Additionally, both methods can be valuable in epidemiological studies following enterococcus resistance gene transfer within human population. Keywords: Enterococcus, identification, D-alanin-D-alanin ligase PCR, Internal Transcribed Spacer PCR Journal: Veterinární medicína Pages: 97-105 Volume: 55 Issue: 3 Year: 2010 DOI: 10.17221/159/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/159/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201003-0001.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:3:id:159-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: V. Fichtelova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Kovarcik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Characterization of two BHV-4 strains isolated in the Czech Republic Abstract: This study describes the isolation of bovine herpesvirus 4 (BHV-4) from the respiratory tract of animals suffering from respiratory disease. DNA of new isolates, CH and Ni, was cleaved with BamHI, EcoRI and HindIII in restriction enzyme analysis and the fragments were identified by co-migration with the restriction profile of the reference strain Movar 33/63 cleaved with the appropriate endonuclease. Typical profiles with polyrepetitive DNA (prDNA) fragments were detected. In order to localize the size variation within the obtained digestion fragments, Southern blot hybridization was performed. Differences between the isolates CH, Ni were localized in both the prDNAs and the unique central part of the genome and were restricted to fragment size variation. Keywords: Bovine herpesvirus 4 (BHV-4), Restriction endonuclease analysis, Southern blot Journal: Veterinární medicína Pages: 106-112 Volume: 55 Issue: 3 Year: 2010 DOI: 10.17221/18/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/18/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201003-0002.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:3:id:18-2010-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Ishii Author-Workplace-Name: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan Author-Name: T. Aoki Author-Workplace-Name: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan Author-Name: K. Yamakawa Author-Workplace-Name: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan Author-Name: T. Uyama Author-Workplace-Name: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan Author-Name: S. El-khodery Author-Workplace-Name: Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt Author-Name: M. Matsui Author-Workplace-Name: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan Author-Name: Y. Miyake Author-Workplace-Name: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan Title: Uterine prolapse in cows: Effect of raising the rear end on the clinical outcomes and reproductive performance Abstract: The aim of this study was to evaluate the effect of raising the rear end when replacing the prolapsed uterus in cows on the outcome and reproductive performance. A total of 76 cows with uterine prolapse were investigated, 10 of which had died before treatment. According to the posture, cows were divided into standing (Standing: n = 11) and recumbent (Recumbent: n = 55). Recumbent cows were subdivided randomly into lying (Lying: n = 24), lifting up using a cow-lift (Cow-lift: n = 18), and raising hind legs using a tractor (Raising: n = 13). Recovery rate after treatment of all cows was 60.6%. The recovery rate of Recumbent (52.7%) was significantly (P < 0.01) lower than Standing (100%). Among the recumbent cows, Lying showed the lowest recovery rate (37.5%), which was significantly lower than the recovery rate (64.5%) of the combined Cow-lift and Raising groups. The mean nonpregnant term of Raising (144 days) was significantly (P < 0.05) shorter than of Cow-lift (297 days). In conclusion, the method of replacing the uterus by raising the hind legs during treatment of uterine prolapse in cows could improve the recovery rate and breeding performance. Keywords: cow, raising hind legs, posture, uterine prolapsed, replace Journal: Veterinární medicína Pages: 113-118 Volume: 55 Issue: 3 Year: 2010 DOI: 10.17221/157/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/157/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201003-0003.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:3:id:157-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Kolar Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: J. Bardon Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Workplace-Name: State Veterinary Institute, Olomouc, Czech Republic Author-Name: M. Chroma Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: K. Hricova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: T. Stosova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: P. Sauer Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: D. Koukalova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Title: ESBL and AmpC beta-lactamase-producing Enterobacteriaceae in poultry in the Czech Republic Abstract: A major reason for resistance of Enterobacteriaceae to beta-lactam antibiotics is production of ESBLs and AmpC beta-lactamases. As their more detailed description in poultry is unavailable in the Czech Republic, the presented study aimed at assessing their occurrence and molecular characteristics. A total of 154 composite samples from broilers and 150 cloacal swabs from turkeys were examined. Production of ESBLs was detected in seven Escherichia coli isolates and AmpC enzymes in two E. coli isolates. The most frequent ESBL types were CTX-M-1 and SHV-12 and the most common AmpC enzymes were the CMY-2 types. Keywords: Escherichia coli, ESBL, AmpC, poultry, Czech Republic Journal: Veterinární medicína Pages: 119-124 Volume: 55 Issue: 3 Year: 2010 DOI: 10.17221/165/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/165/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201003-0004.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:3:id:165-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D.-C. Woo Author-Workplace-Name: College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea Author-Name: C.-B. Choi Author-Workplace-Name: Kyunghee University Medical Center, Seoul, Republic of Korea Author-Name: J.-W. Nam Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: K.-N. Ryu Author-Workplace-Name: Kyunghee University Medical Center, Seoul, Republic of Korea Author-Name: G.-H. Jahng Author-Workplace-Name: East-West Neo Medical Center, Kyung-Hee University, Seoul, Korea Author-Name: S.-H. Lee Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: D.-W. Lee Author-Workplace-Name: College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea Author-Name: S.-Y. Kim Author-Workplace-Name: College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea Author-Name: H.-Y. Kim Author-Workplace-Name: College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea Author-Name: K.-J. Ahn Author-Workplace-Name: College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea Author-Name: B.-Y. Choe Author-Workplace-Name: College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea Title: Quantitative analysis of hydrocephalic ventricular alterations in Yorkshire terriers using magnetic resonance imaging Abstract: The purpose of this work was to evaluate hydrocephalic ventricular changes using three quantitative analysis methods. The height, area and volume of the ventricles and brain were measured in 20 Yorkshire terriers (10 normal and 10 hydrocephalic dogs) using low-field MR imaging (at 0.2 Tesla). All measurements were averaged and the relative ventricle size was defined as a percentage (percent size of the ventricle/size of the brain). The difference between normal and hydrocephalic dogs was statistically significant for the average of each ventricle as well as for the percentage value. Five hydrocephalic symptoms were identified: circling, head tilting, seizures, ataxia, and strabismus. With respect to height, area and volume of the brain/ventricle, the difference between normal and hydrocephalic dogs was not significant. The ventricle/brain with height (1D) was related to the area (2D) and volume (3D). The correlations with area and volume were as good as the ventricle/brain height ratio in the case of hydrocephalic dogs. Therefore, one-, two- and three-dimensional quantitative methods may be complementary. We expect that the stage of hydrocephalic symptoms can be classified if statistical significance for ventricular size among symptoms is determined with the analysis of a large number of hydrocephalic cases. Keywords: hydrocephalus, ventricles, magnetic resonance imaging, Yorkshire terrier Journal: Veterinární medicína Pages: 125-132 Volume: 55 Issue: 3 Year: 2010 DOI: 10.17221/127/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/127/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201003-0005.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:3:id:127-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Matos Author-Workplace-Name: INRB, IP-LNIV, Animal Health Unit, Bacteriology Laboratory, Lisboa, Portugal Author-Name: A. Amado Author-Workplace-Name: INRB, IP-LNIV, Animal Health Unit, Bacteriology Laboratory, Lisboa, Portugal Author-Name: A. Botelho Author-Workplace-Name: INRB, IP-LNIV, Animal Health Unit, Bacteriology Laboratory, Lisboa, Portugal Title: Molecular typing of Mycobacterium bovis isolated in the first outbreak of bovine tuberculosis in the Azores Islands: a case report Abstract: Twenty Mycobacterium bovis isolates from a first reported outbreak in the Azores Islands were differentiated into four spoligotypes: SB0119 (45%), SB0121 (40%), SB1264 (10%) and SB1090 (5%) and into two MIRU-VNTR allelic profiles using eight selected loci. The isolates seem to constitute a clonal lineage from a common source of infection. The limited diversity among the analysed Azores strains isolates could be due to the close contact of animals and use of common pastures and all herds can be seen as one epidemiological unit. The population structure of these strains with its low diversity differs from the Portugese mainland indicating a recent infection with accompanying evident founder effect. Keywords: spoligotyping, MIRU-VNTR typing, Mycobacterium bovis Journal: Veterinární medicína Pages: 133-136 Volume: 55 Issue: 3 Year: 2010 DOI: 10.17221/137/2009-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/137/2009-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201003-0006.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:3:id:137-2009-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Lew Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: S. Lew Author-Workplace-Name: Faculty of Biology, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Rozicka Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Title: Upper eyelid and medial canthus reconstructive surgery after histiocytoma resection in a dog: a case report Abstract: A six year old crossbred male dog presented with non-pigmented, pale pink, unhaired, ulcerative tumor of the medial canthus and upper eyelid region. As treatment, surgery was proposed. During the surgery complete excision of the tumor was performed. The extent of skin loss, especially in the upper eyelid required plastic reconstruction by pedicle flap transposition. The soft skin of the cheek was chosen as a graft donor site. The postoperative period proceeded without any complications and ended with successful engraftment. The soft cheek skin did not impede movement of the upper eyelid. Histopathological examination indicated histiocytoma and tumor-free excisional margins were confirmed. There has been no recurrence during the subsequent 12 month period. Keywords: reconstructive surgery, blepharoplasty, transposition flap, histiocytoma, dog Journal: Veterinární medicína Pages: 137-143 Volume: 55 Issue: 3 Year: 2010 DOI: 10.17221/69/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/69/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201003-0007.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:3:id:69-2010-VETMED