Template-Type: ReDIF-Article 1.0 Author-Name: R. Krametter-Froetscher Author-Name: N. Mason Author-Name: J. Roetzel Author-Name: V. Benetka Author-Name: Z. Bago Author-Name: K. Moestl Title: Effects of Border disease virus (genotype 3) naturally transmitted by persistently infected sheep to pregnant heifers and their progeny Abstract: Eight heifers pregnant between days 47 and 73 were kept together with nine healthy persistently Border disease virus (BDV)-infected sheep allowing natural contact comparable to field conditions. All heifers seroconverted between days 23 and 38 after exposure. Besides a mild increase in body temperature in four heifers, no clinical signs of infection were observed, but 5 animals aborted between days 54 and day 202 after exposure. BDV was detected in the aborted fetuses of four heifers and in the placenta of the fifth (the only material available). Foetal mummification was seen in three foetuses, aborted between days 113 and 116 of gestation, with a crown rump length (CRL) of between 11 and 12 cm. The associated placentas showed dystrophic calcification. The foetus aborted on day 267 of gestation had a CRL of 70 cm and a body mass of 16 kg. The brain in this case was normal in terms of gross morphology, but histologically slight lymphocytic meningeal and perivascular infiltration, slight demyelination in the cerebellar white matter and slight focal acute liquefactive necrosis in the thalamus were seen. Three heifers delivered clinically healthy calves. Two healthy calves were pestivirus negative, of these one was serologically positive and one negative (precolostral). The third calf was pestivirus positive and antibody negative at birth, suggesting immunotolerance and persistent infection as has been described for BVDV. But surprisingly when retested at an age of seven months the calf had seroconverted and was pestivirus negative. Post-mortem examination of the heifers and the calves born alive revealed no abnormalities, pestivirus specific RNA was not detected in any of the examined organ samples of the eight heifers and the three calves. In this study, BDV-type 3 infection had a disastrous impact on fertility of pestivirus naïve heifers. The observed abortion rate exceeded 50% and dramatically underlines the need for a pestivirus control program which includes BDV and small ruminants. Keywords: Border disease virus, abortion, sheep, BVDV eradication, cattle Journal: Veterinární medicína Pages: 145-153 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/29/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/29/2010-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:55:y:2010:i:4:id:29-2010-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Antosik Author-Workplace-Name: University of Life Sciences, Poznan, Poland Author-Name: B. Kempisty Author-Workplace-Name: University of Medical Sciences, Poznan, Poland Author-Name: M. Jackowska Author-Workplace-Name: University of Life Sciences, Poznan, Poland Author-Name: D. Bukowska Author-Workplace-Name: University of Life Sciences, Poznan, Poland Author-Name: M. Lianeri Author-Workplace-Name: University of Medical Sciences, Poznan, Poland Author-Name: K.P. Brussow Author-Workplace-Name: Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany Author-Name: M. Wozna Author-Workplace-Name: University of Life Sciences, Poznan, Poland Author-Name: J.M. Jaskowski Author-Workplace-Name: University of Life Sciences, Poznan, Poland Title: The morphology of porcine oocytes is associated with zona pellucida glycoprotein 3 and integrin beta 2 protein levels-- Abstract: The morphology and quality of oocytes is set during oo- and folliculo-genesis, and is completed during final maturation. Early embryonic development is associated with the morphology of the cumulus-oocyte-complex (COC). However, current knowledge of the possible relationships between oocyte morphology and the level of proteins within the oocyte, which may reflect fertilization ability, is insufficient. Using western-blot analysis and confocal microscopic observation, we determined the levels of integrin beta-2 (integrin β2) and glycoprotein 3 (pZP3) protein levels in the porcine zona pellucida of four morphologically different types of oocytes, graded according to their cytoplasmic composition and cumulus structure. The level of integrin β2 protein was increased in grade I and II oocytes as compared to other grades (P < 0.05). Moreover, the level of pZP3 protein was 3-4 fold higher in grade I oocytes (P < 0.01). We suggest that COC morphology may be associated with oocyte fertilization ability with respect to its sperm-oocyte interaction gene expression, which is the result of increased accumulation of specific proteins prior to fertilization in higher quality oocytes. Higher quality oocytes may also reflect better fertilization ability. Keywords: pig, oocyte, morphology, glycoproteins, integrins Journal: Veterinární medicína Pages: 154-162 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/38/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/38/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201004-0002.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:4:id:38-2010-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Crnogaj Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: R. Petlevski Author-Workplace-Name: Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia Author-Name: V. Mrljak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: I. Kis Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M. Torti Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: N. Kucer Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: V. Matijatko Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: I. Sacer Author-Workplace-Name: Veterinary Station of the City of Zagreb, Zagreb, Croatia Author-Name: I. Stokovic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Malondialdehyde levels in serum of dogs infected with Babesia canis Abstract: Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory data the 35 infected dogs were clinically classified into two groups, complicated (seven dogs) and uncomplicated (28 dogs). The noted complications were renal dysfunction (5/7), hepatic dysfunction (3/7), muscular involvement (2/7) and ARDS (1/7). Levels of blood urea nitrogen concentration (BUN), creatinin, total bilirubin, alanin aminotranspherase (ALT), alkaline phosphatase (AP), and gammaglutamil aminotranspherase (GGT) were significantly increased in dogs with complicated versus uncomplicated babesiosis. Furthermore the uncomplicated group of dogs was, depending on the severity of anaemia, classified as suffering from severe, moderate or mild disease. Levels of serum MDA were significantly higher in sick dogs (36.90 μmol/l ± 13.95) than healthy animals (8.13 μmol/l ± 1.78). There was no significant statistical difference in serum MDA levels between dogs with complicated (38.48 μmol/l ± 12.11) and uncomplicated babesiosis (36.50 μmol/l ± 14.55). Comparison of the groups based on the severity of anaemia showed that there was no significant statistical difference in serum MDA levels between them. The study demonstrated the involvement of oxidative damage in dogs naturally infected with B. canis. Keywords: babesiosis, dog, oxidative stress, malondialdehyde (MDA) Journal: Veterinární medicína Pages: 163-171 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/77/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/77/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201004-0003.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:4:id:77-2010-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S.-J. Du Author-Workplace-Name: Graduate Institute of Veterinary Medicine, National Taiwan University, Taiwan, ROC Author-Name: H.-C. Kuo Author-Workplace-Name: Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan, ROC Author-Name: C.-H. Cheng Author-Workplace-Name: Graduate Institute of Veterinary Medicine, National Taiwan University, Taiwan, ROC Author-Name: A.C.Y. Fei Author-Workplace-Name: Graduate Institute of Veterinary Medicine, National Taiwan University, Taiwan, ROC Author-Name: H.-W. Wei Author-Workplace-Name: Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan, ROC Author-Name: S.-K. Chang Author-Workplace-Name: Graduate Institute of Veterinary Medicine, National Taiwan University, Taiwan, ROC Title: Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections Abstract: Sixty-six clinical P. aeruginosa isolates, 17 obtained from canine otitis specimens and 49 received from human patients with bloodstream infections, were collected between February 2007 and January 2008. The minimal inhibitory concentrations (MICs) of the antimicrobial agents of these isolates were determined. Multidrug resistance was common, with 23 (34.8%) isolates found to be ceftazidime resistant. To explore the mechanisms of ceftazidime resistance, PCR analyses were performed to detect drug-resistance genes. The prevalence rate of Ambler class A, B, and D β-lactamase genes were obtained, with blaTEM-1 100%, blaPSE-1 100%, blaOXA-2 96.2%, blaSHV-18 91.3%, blaOXA-17 78.3%, blaVIM-3 26.1%, blaOXA-10 21.7% and blaSHV-1 8.7%. An efflux inhibition assay with the PAβN compound was conducted. The ceftazidime resistance isolates were also tested by RT-qPCR to determine the mRNA expression levels of the oprM and ampC genes. Five (21.7%) of the ceftazidime resistance isolates appeared to overactivate the OprM efflux system. The ampD, ampE, and ampR genes and the ampC-ampR intergenic region were subsequently amplified and sequenced. Five (21.7%) of the ceftazidime resistance isolates from humans and canines had a point mutation in AmpR (Asp135-Asn, n = 3; Als194-Ser, n = 2), which induces AmpC overproduction from 10- to 80-fold. This study first reported ceftazidime resistance in P. aeruginosa from canine otitis specimens, which are closely related to ESBLs (50%), including the overproduction of AmpC (25%) and the OprM efflux system (25%). The ESBLs (100%) played an important role in all ceftazidime resistance isolates from humans, and either AmpC (21.1%) or OprM (21.1%) might be overexpressed within the same isolate. A human patient isolate (H307B) showed simultaneous expression of ESBLs, the OprM efflux system, and AmpC overproduction. Keywords: ampC, ampR, extended spectrum β -lactamases, OprM efflux system Journal: Veterinární medicína Pages: 172-182 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/64/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/64/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201004-0004.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:4:id:64-2010-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Aydin Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Title: The spinal nerves that constitute the plexus lumbosacrales of the red squirrel (Sciurus vulgaris) Abstract: This study was aimed at investigating the lumbosacral plexus of squirrels (Sciurus vulgaris). Ten squirrels were used in this work. The animals were carefully dissected and the spinal nerves that constitute the lumbosacral plexus were examined. It was discovered that the lumbosacral plexus was formed by L6, L7, S1, S2 in nine animals and in the one remaining animal in addition to these branches rami ventralis of L4 and L5 were forming the plexus. The common root forming the last part of the lumbosacral plexus and giving branches dispersing to the hind limb was formed by the rami ventralis of L6, L7, S1, S2. Thus, in squirrels, the spinal nerves forming the lumbosacral plexus and the joining of these spinal nerves to each other differs from other rodents and mammals. Keywords: lumbosacral plexus, nerves, the red squirrel (Sciurus vulgaris) Journal: Veterinární medicína Pages: 183-186 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/3021-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/3021-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201004-0005.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:4:id:3021-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Bodecek Author-Workplace-Name: Veterinary Faculty, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Jahn Author-Workplace-Name: Veterinary Faculty, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: O. Dobesova Author-Workplace-Name: Veterinary Faculty, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: E. Vavrouchova Author-Workplace-Name: Veterinary Faculty, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Equine cyathostomosis: case reports Abstract: Twelve clinical cases of cyathostomosis in horses treated at the Equine Clinic University of Veterinary and Pharmaceutical Sciences in Brno, the Czech Republic, between the years 1999 and 2008 are described in this report. Six cases (50%) were hospitalized in the period from 2007 to 2008. Eleven of them were hospitalized in the period from December to March. Only one case was admitted in June, but the clinical signs had appeared for the first time in January. All horses described in these cases were younger than six years of age. Diarrhoea as a predominant clinical sign was present in four horses and colic in four horses. One horse showed both colic and diarrhoea whilst three horses had weight loss and subcutaneous oedema. Metabolic acidosis was found in three horses, eight patients had leucocytosis. Hypoproteinemia was found in four horses, hypoalbuminemia in seven horses, hypokalemia in three horses and increased alkaline phosphatase (ALP) activity in five horses. Seven horses recovered, one horse died and four horses were euthanized. Keywords: cyathostome infection, small strongyles, diarrhoea, colic, hypoalbuminemia Journal: Veterinární medicína Pages: 187-193 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/88/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/88/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201004-0006.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:4:id:88-2010-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: O. Smolec Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J. Kos Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: D. Vnuk Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M. Stejskal Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: N. Brkljaca Bottegaro Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: R. Zobel Author-Workplace-Name: Veterinary Practice, Struzec, Croatia Title: Multiple congenital malformation in a Simental female calf: a case report Abstract: Congenital anomalies in offspring of natural breedings are often a result of environmental factors, genetic factors, or both. A 21-day-old male Simental female calf was submitted to our clinic with abnormal severe green mucoid nasal discharge from both nostrils. At necropsy, the calf showed multiple defects, including cleft palate, hydrocephalus, diphragmatic hernia and freemartinism. The cause of these anomalies could not be determined. This report is the first to describe these multiple malformations in a single calf. Keywords: Simental, calf, malformations Journal: Veterinární medicína Pages: 194-198 Volume: 55 Issue: 4 Year: 2010 DOI: 10.17221/95/2010-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/95/2010-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201004-0007.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:4:id:95-2010-VETMED