Template-Type: ReDIF-Article 1.0 Author-Name: A. Aksakal Author-Workplace-Name: Faculty of Veterinary Science, Yuzuncu Yil University, Van, Turkey Title: Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE Abstract: This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1, 33.9, 30.7, 27.6, 25.4, and 24 kDa were detected in all Salmonella serovars. Salmonella strains used in this study were closely related and could not be differentiated depending on the whole cell protein profiles using SDS-PAGE. Keywords: whole cell protein, Salmonella, chicken, turkey, sheep, SDS-PAGE Journal: Veterinární medicína Pages: 259-263 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2986-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2986-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0001.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2986-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D. Mista Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: J. Piekarska Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: M. Houszka Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: W. Zawadzki Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Author-Name: M. Gorczykowski Author-Workplace-Name: Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Title: The influence of orally administered short chain fatty acids on intestinal histopathological changes and intensity of Trichinella spiralis infection in mice Abstract: The influence of short chain fatty acids (SCFA) on histopathological changes in the small intestine and the intensity of invasion of T. spiralis in mice were investigated in this study. The animals were infected with doses of 500 and 250 T. spiralis larvae per mouse. A SCFA solution containing acetic, propionic and butyric acid (30 : 15 : 20mM) was administered orally to the mice starting from the 5th day before infection to the 20th day after infection (day). Fragments of the jejunum collected during dissection on the 7th and 10th day were used to prepare specimens to assess the histopathological changes. In the infected animals, the intestinal trichinellae were counted on the 7th and 10th day, while on the 42nd day the muscle larvae number were determined. The strongest host reaction in the intestine was observed on the 7th day at a dose of T. spiralis 500 larvae, and on the 10th day at a dose of 250 larvae. Numerous inflammatory infiltrations, strong shortening of the intestinal villi, extension of the intestinal crypts, and the lowest ratio of the villi length to the intestinal crypts depth were observed. The ratio was 1.3 ± 0.3 on the 7th day at a dose of 500 larvae, and on the 10th day, at dose of 250 larvae the ratio reached 1.5 ± 0.5. Both values differed significantly from the control group: 3.3 ± 0.5 (P < 0.01). Administration of SCFA to the animals infected with T. spiralis caused remission of local histopathological changes resulting from the presence of the parasite in the small intestine after the mentioned periods. This manifested as limited villi shortening and reduced deepening of intestinal crypts. At the higher infectious dose, in animals receiving the acid solution, on the 7th day the intestinal villi were considerably longer (356 µm ± 35) than in the group infected with T. spiralis but not treated with the acids (279 µm ± 57; P < 0.01). At a lower dose of parasites, on the 10th day these values were 339 µm ± 88 and 306 µm ± 47 respectively and the observed differences were not statistically significant. The solution of SCFA also caused a decrease in the numbers of mature parasites in the intestine and the muscle larvae at a dose of 500 larvae/mouse. In animals receiving the SCFA, 24 050 ± 10 415 larvae were observed in muscles, while in the infected mice, which did not receive the acids, 32 875 ± 16 762 larvae were detected (P < 0.05). An increase in the intensity of infection accelerated the rate of host reaction to the presence of T. spiralis in the intestines (self-cure). To summarize, the administered solution of short chain fatty acids alleviated the formation of histopathological changes in the intestine in response to the parasite's presence, and lowered the intensity of T. spiralis invasion after infection with a higher dose of larvae. Keywords: trichinellosis, SCFA, intestinal morphology, mouse Journal: Veterinární medicína Pages: 264-274 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2992-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2992-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0002.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2992-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Medical and Bio-safety Research Institute, Chonbuk National University, Jeonju, Korea Author-Name: H.C. Kim Author-Workplace-Name: College of Veterinary Medicine, Kangwon National University, Chuncheon, Korea Author-Name: C. Sim Author-Workplace-Name: Department of Biology, Baylor University, Waco, Texas, USA Author-Name: J.R. Ji Author-Workplace-Name: Paek Kwang C&S Co. LTD, Sehyun-dong Bundang-gu Sungnam City, Korea Author-Name: N.S. Kim Author-Workplace-Name: Research Institute of Veterinary Science and College of Veterinary Medicine, Chungnam National University, Daejeon, Korea Author-Name: B.K. Park Author-Workplace-Name: Research Institute of Veterinary Science and College of Veterinary Medicine, Chungnam National University, Daejeon, Korea Title: Congenital infection with Setaria digitata and Setaria marshalli in the thoracic cavity of a Korean calf: a case report Abstract: In March 2010, a 3.5-month-old Korean native calf was anatomized and two nematode worms were detected in the thoracic cavity. The worms were identified and classified by light and scanning electron microscopy on the basis of features at the anterior and posterior parts of the worms. The worms were female Setaria digitata and Setaria marshalli and numerous eggs which contained microfilaria were detected in the uterus of both species. The body lengths of the S. digitata and S. marshalli were 78 mm and 117 mm, respectively. Mosquitoes act as the vector for Setaria nematodes but these are inactivated in winter in Korea. Therefore, we concluded that this case represented setariasis with congenital infection occurring during the summer prenatal stage of life. Keywords: Setaria digitata, Setaria marshalli, Korean native calf, congenital infection Journal: Veterinární medicína Pages: 275-280 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2987-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2987-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0003.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2987-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F.J. Mendoza Author-Workplace-Name: Department of Animal Medicine and Surgery, University of Cordoba, Spain Author-Name: A. Perez-Ecija Author-Workplace-Name: Department of Animal Medicine and Surgery, University of Cordoba, Spain Author-Name: J.C. Estepa Author-Workplace-Name: Department of Animal Medicine and Surgery, University of Cordoba, Spain Title: Inguinal-scrotal herniation and torsion of the large colon in an adult Andalusian stallion: a case report Abstract: A 3-year-old Andalusian stallion was referred to the Veterinary Teaching Hospital of the University of Cordoba due to acute onset of colic pain. At admission, the horse presented a pronounced distension of the inguinal and scrotal region which was cold and painful on palpation. On rectal palpation no small intestine gas distention was noticed, and the left large colon was detected protruding into the left internal inguinal ring. The percutaneous scrotal sonogram showed an intestinal loop which had moved into the scrotal bag close to the left testicle. The horse was diagnosed with indirect acquired left inguinal hernia. The necropsy revealed an impacted and twisted pelvic flexure in the vaginal space. To the authors' knowledge, this report is the first that describes a clinical case of inguinal-scrotal herniation and torsion of the pelvic flexure into the inguinal canal in an adult stallion associated with an impaction of the ascending colon. Keywords: inguinal hernia, pelvic flexure, stud, impaction Journal: Veterinární medicína Pages: 281-284 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2993-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2993-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0004.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2993-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Kozdrowski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life and Environmental Science, Wroclaw, Poland Author-Name: R. Henklewski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life and Environmental Science, Wroclaw, Poland Author-Name: M. Dzieciol Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life and Environmental Science, Wroclaw, Poland Author-Name: M. Bednarski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Life and Environmental Science, Wroclaw, Poland Title: A large urolith in an eight year-old mare: a case report Abstract: A micturition problem appeared in an eight year-old mare at the third month of pregnancy. Symptoms intensified during the course of pregnancy and, at the end of the tenth month, the foetus was aborted. Labour was ordinary with normal presentation, position and posture, yet there was a formation about the size of an ostrich egg. The structure was spontaneously pushed out of the genital tract during expulsion of the foetus. Examination of the structure showed that it was a urolith which measured 13 × 10 × 7 cm (circumference measured along the long axis amounted to 31.5 cm, and along the transverse axis to 26 cm) and weighed 803 g. Keywords: horse, abortion, urolith, urinary tract, urine Journal: Veterinární medicína Pages: 285-288 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2990-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2990-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0005.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2990-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Blutke Author-Workplace-Name: Institute of Veterinary Pathology, Ludwig-Maximilians-University Munich, Munich, Germany Author-Name: B. Parzefall Author-Workplace-Name: Small Animal Referral Practice Staufeneck, Piding, Germany Author-Name: A. Steger Author-Workplace-Name: Small Animal Referral Practice Staufeneck, Piding, Germany Author-Name: T. Goedde Author-Workplace-Name: Small Animal Referral Practice Staufeneck, Piding, Germany Author-Name: W. Hermanns Author-Workplace-Name: Institute of Veterinary Pathology, Ludwig-Maximilians-University Munich, Munich, Germany Title: Inflammatory polyp in the middle ear of a dog: a case report Abstract: Nasopharyngeal polyps are non-neoplastic masses, originating from the mucosa of the nasopharynx, the tympanic bulla or the Eustachian tube. Inflammatory polyps extending into the tympanic bulla cavity are a common cause of otitis media in cats. In dogs, however, occurrence of middle ear polyps has rarely been reported. The present report describes the findings of the clinical examination, diagnostic imaging and histopathological appraisal of a ten year old male dog with an inflammatory middle ear polyp arising from the mucosa of the Eustachian tube. Clinically, the dog displayed a peripheral vestibular syndrome. Magnetic resonance imaging revealed a hyperintense soft tissue mass filling the right middle ear cavity. Following ventral bulla osteotomy, a polypoid growth with a stalk arising from the auditory tube was surgically excised from the tympanic bulla. Histologically, the polyp was composed of a fibrous connective tissue stroma with discreet infiltration of inflammatory cells and an overlying surface layer of partially ulcerated respiratory epithelium. Similarities and differences between the histological appearance of the present case and the few previously reported records of canine middle ear polyps are discussed, along with a comparative review of etiological, pathogenetic and therapeutic aspects of middle ear polyps in cats and dogs Keywords: nasopharynx, otitis, polyp, tympanic bulla Journal: Veterinární medicína Pages: 289-293 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2991-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2991-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0006.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2991-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J. Habus Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: Z. Stritof Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: V. Stevanovic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: K. Martinkovic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: Z. Milas Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: V. Staresina Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Author-Name: N. Turk Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Croatia Title: Dual infection of rabies virus and Babesia canis in a dog: a case report Abstract: A young adult labrador was presented for evaluation of hind leg weakness, depression, vomiting and severe icterus of skin and mucosa. Physical examination and laboratory findings suggested cerebral babesiosis. Unresponsiveness to treatment and unknown vaccinal status aroused suspicion of rabies which was confirmed post mortem. Keywords: rabies virus, Babesia canis, dog Journal: Veterinární medicína Pages: 294-296 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2989-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2989-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0007.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2989-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Alvarez-Perez Author-Workplace-Name: Faculty of Veterinary, Universidad Complutense de Madrid, Spain Author-Name: A. Mateos Author-Workplace-Name: VISAVET, Health Surveillance Centre, Universidad Complutense de Madrid, Spain Author-Name: L. Dominguez Author-Workplace-Name: VISAVET, Health Surveillance Centre, Universidad Complutense de Madrid, Spain Author-Name: E. Martinez-Nevado Author-Workplace-Name: Zoo-Aquarium, Madrid, Spain Author-Name: J.L. Blanco Author-Workplace-Name: Faculty of Veterinary, Universidad Complutense de Madrid, Spain Author-Name: M.E. Garcia Author-Workplace-Name: Faculty of Veterinary, Universidad Complutense de Madrid, Spain Title: Isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens): a case report Abstract: This paper reports the isolation of Rhodotorula mucilaginosa from skin lesions in a Southern sea lion (Otaria flavescens). The microorganism was isolated from cutaneous lesions, identified by the commercial API 20 C AUX system, and confirmed by sequencing. Topical treatment with sertaconazol resulted in complete clinical recovery of the animal and repeat testing did not result in the recovery of the yeast from the healed lesion sites. Keywords: dermatomycoses, rhodotorulosis, sertaconazol, pinnipeds, yeast Journal: Veterinární medicína Pages: 297-301 Volume: 55 Issue: 6 Year: 2010 DOI: 10.17221/2988-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2988-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201006-0008.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:6:id:2988-VETMED