Template-Type: ReDIF-Article 1.0 Author-Name: M. Atef Yekta Author-Workplace-Name: Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium Author-Name: F. Verdonck Author-Workplace-Name: Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium Author-Name: W. Van Den Broeck Author-Workplace-Name: Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium Author-Name: B.M. Goddeeris Author-Workplace-Name: Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium Author-Workplace-Name: Faculty of Bioscience Engineering, Catholic University, Leuven, Belgium Author-Name: E. Cox Author-Workplace-Name: Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium Author-Name: D. Vanrompay Author-Workplace-Name: Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium Title: Lactoferrin inhibits E. coli O157:H7 growth and attachment to intestinal epithelial cells Abstract: Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strains are associated with haemorraghic colitis and haemolytic uremic syndrome (HUS) in humans. Cattle are a reservoir of E. coli O157:H7. We studied the ability of bovine and human lactoferrin, two natural antimicrobial proteins present in milk, to inhibit E. coli O157:H7 growth and attachment to a human epithelial colorectal adenocarcinoma cell line (Caco-2). The direct antibacterial effect of bLF on E. coli O157:H7 was stronger than that of hLF. Nevertheless, both lactoferrins had bacteriostatic effects even at high concentrations (10 mg/ml), suggesting blocking of LF activity by a yet undefined bacterial defence mechanism. Additionally, both lactoferrins significantly inhibited E. coli O157:H7 attachment to Caco-2 cells. However, hLF was more effective than bLF, probably due to more efficient binding of bLF to intelectin present on human enterocytes leading to uptake and thus removal of bLF from the extracellular environment. Inhibition of bacterial attachment to Caco-2 cells was at least partly due to the catalytic effect of lactoferrins on the type III secreted proteins EspA and EspB Keywords: transferring, type III secretion system, EspA, EspB Journal: Veterinární medicína Pages: 359-368 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2954-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2954-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0001.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2954-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Slany Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Svobodova Author-Workplace-Name: Regional Institute of Public Health, Brno, Czech Republic Author-Name: A. Ettlova Author-Workplace-Name: BioPlus, s.r.o., Brno, Czech Republic Author-Name: I. Slana Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: V. Mrlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Mycobacterium arupense among the isolates of non-tuberculous mycobacteria from human, animal and environmental samples Abstract: Mycobacterium arupense is a non-tuberculous, potentially pathogenic species rarely isolated from humans. The aim of the study was to ascertain the spectrum of non-tuberculous mycobacteria within 271 sequenced mycobacterial isolates not belonging to M. tuberculosis and M. avium complexes. Isolates were collected between 2004 and 2009 in the Czech Republic and were examined within the framework of ecological studies carried out in animal populations infected with mycobacteria. A total of thirty-three mycobacterial species were identified. This report describes the isolation of M. arupense from the sputum of three human patients and seven different animal and environmental samples collected in the last six years in the Czech Republic: one isolate from leftover refrigerated organic dog food, two isolates from urine and clay collected from an okapi (Okapia johnstoni) and antelope bongo (Tragelaphus eurycerus) enclosure in a zoological garden, one isolate from the soil in an eagle's nest (Haliaeetus albicilla) band two isolates from two common vole (Microtus arvalis) livers from one cattle farm. All isolates were identified by biochemical tests, morphology and 16S rDNA sequencing. Also, retrospective screening for M. arupense occurrence within the collected isolates is presented. Keywords: 16S rDNA sequencing, non-tuberculous mycobacteria, ecology Journal: Veterinární medicína Pages: 369-376 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2956-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2956-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0002.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2956-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A.V. Sirotkin Author-Workplace-Name: Animal Production Research Centre, Nitra, Slovak Republic Author-Name: M. Kacaniova Author-Workplace-Name: University of Agriculture, Nitra, Slovak Republic Title: The effect of high temperature on swine ovarian function in vitro Abstract: The aim of the present study was to understand the hormonal mechanisms behind the effect of high temperatures on reproductive function. It was proposed that high temperatures can directly alter production of ovarian hormones and/or the response of ovarian cells to hormonal stimulators. To examine this hypothesis, in the 1st series of experiments, we compared the release of progesterone (P4), estradiol (E2) and expression of the leptin gene in whole ovarian follicles cultured in conditions of normal (37.5°C) and high (41.5°C) temperatures. In the 2nd series of experiments, we examined the release of P4 and insulin-like growth factor I (IGF-I) by ovarian granulosa cells cultured in conditions of normal and high temperatures with and without IGF-I, leptin and FSH. The release of hormones was measured by RIA, while the expression of the leptin gene was evaluated by PCR. It was observed that high temperature significantly increased P4 and E2 release and reduced the accumulation of leptin DNA in ovarian follicles. In cultured ovarian granulosa cells, high temperatures promoted the release of both P4 and IGF-I. The addition of IGF-I, leptin and FSH to granulosa cells cultured at normal temperature promoted the release of both P4 and IGF-I. High temperature was able to prevent the stimulatory effect of leptin (but not of IGF-I or FSH) on P4 output and the stimulatory action of both leptin and FSH on IGF-I release by granulosa cells. The present observations (1) demonstrate the possible production of leptin in the porcine ovary, (2) demonstrate for the first time the influence of high temperatures on ovarian P4, E2, IGF-I and leptin, and (3) suggest, that the negative effect of heat stress on reproductive processes can be due to high temperature-induced malproduction of ovarian hormones and a reduction in the response of ovarian cells to hormonal stimulators. Keywords: temperature, IGF-I, leptin, FSH, progesterone, estradiol, ovaries, pig Journal: Veterinární medicína Pages: 377-382 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2953-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2953-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0003.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2953-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: J.G. Chediack Author-Workplace-Name: Laboratory of Biology "Prof. E. Caviedes Codelia", Facultad de Ciencias Humanas, Universidad Nacional de San Luis, San Luis, Argentina Author-Workplace-Name: Laboratory of Integrative Biology, Institute for Multidisciplinary Research in Biology (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas, San Luis, Argentina Author-Workplace-Name: Dept. Biochemistry and Biological Sciences, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina Author-Name: F.D. Cid Author-Workplace-Name: Laboratory of Biology "Prof. E. Caviedes Codelia", Facultad de Ciencias Humanas, Universidad Nacional de San Luis, San Luis, Argentina Author-Workplace-Name: Laboratory of Integrative Biology, Institute for Multidisciplinary Research in Biology (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas, San Luis, Argentina Author-Workplace-Name: Dept. Biochemistry and Biological Sciences, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina Author-Name: S.V. Fasulo Author-Workplace-Name: Laboratory of Biology "Prof. E. Caviedes Codelia", Facultad de Ciencias Humanas, Universidad Nacional de San Luis, San Luis, Argentina Author-Workplace-Name: Laboratory of Integrative Biology, Institute for Multidisciplinary Research in Biology (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas, San Luis, Argentina Author-Name: E. Caviedes-Vidal Author-Workplace-Name: Laboratory of Biology "Prof. E. Caviedes Codelia", Facultad de Ciencias Humanas, Universidad Nacional de San Luis, San Luis, Argentina Author-Workplace-Name: Laboratory of Integrative Biology, Institute for Multidisciplinary Research in Biology (IMIBIO-SL), Consejo Nacional de Investigaciones Científicas y Técnicas, San Luis, Argentina Author-Workplace-Name: Dept. Biochemistry and Biological Sciences, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina Title: Chronic cannulation in the small intestine of feral pigeons (Columba livia) to assess bioavailability Abstract: We improved a method of chronic duodenal cannulation to study intestinal transport of solutes in an in vivo model (pigeon, Columba livia). A hypoallergenic cannula was inserted into the proximal part of the small intestine of pigeons and used for solution administration. Recovery from surgery was extremely rapid and animals started eating and drinking within a day. After surgery, the body mass of cannulated pigeons was stable, and no adverse effects in the weight could be detected. The method is simple, economical and useful to determine intestinal bioavailability of solutes, for nutritional and ecological studies, in intact animals without influence of anesthesia. Keywords: chronic cannulation, small intestine, pigeons, bioavailability Journal: Veterinární medicína Pages: 383-388 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2957-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2957-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0004.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2957-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H.H. Ari Author-Workplace-Name: Veterinary Faculty, University of Yuzuncu Yil, Van, Turkey Author-Name: Z. Soyguder Author-Workplace-Name: Veterinary Faculty, University of Yuzuncu Yil, Van, Turkey Author-Name: S. Cinaroglu Author-Workplace-Name: Veterinary Faculty, University of Yuzuncu Yil, Van, Turkey Title: Macroanatomy of the cranial cervical ganglion in Angora goats Abstract: The cranial cervical ganglia (CCG) in the heads of six adult (three male and three female) Angora goats were dissected in detail. The ganglion was located on the ventral aspect of the tympanic bulla, cranio-ventral to the atlas, medio-ventral to the jugular process and lateral to the longus capitis muscle. The branches of the ganglion were the internal and external carotid nerves, the jugular and laryngopharyngeal nerves and the connecting branches to the vagus and glossopharyngeal nerve. The internal carotid nerve arose as three branches (cranial, caudal and medial) from the cranial region of the ganglion. The cranial branch, the profound petrosal nerve, entered the pterygoid canal. The caudal branch terminated at the trigeminal ganglion. The medial branch terminated at the cavernous sinus. The other cranial branch ramifying from the cranial region of the ganglion was the jugular nerve. The internal carotid and laryngopharyngeal nerves arose from the caudal region of the ganglion. In conclusion, compared with published data on other species, we found differences in the number and courses of the branches ramifying from the CCG of Angora goats and in the branches connected to the vagus, glossopharyngeal and hypoglossal nerves. Keywords: anatomy, cranial cervical ganglion (CCG), Angora goat Journal: Veterinární medicína Pages: 389-393 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2952-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2952-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0005.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2952-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Tofant Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: M. Ostovic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: S. Wolf Author-Workplace-Name: Rovisce Pig-Breeding Farm, Ltd., Rovisce, Croatia Author-Name: A. Ekert Kabalin Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: Z. Pavicic Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Author-Name: J. Grizelj Author-Workplace-Name: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Title: Association between over-chlorinated drinking water and adverse reproductive outcomes in gilts and sows: a case report Abstract: The present report describes a series of adverse events recorded in gilts and sows at a pig-breeding farm. The animals were listless, had poor appetite and dyspnea, sneezed and coughed, but were afebrile. Subsequently, an increased number of spontaneous abortions occurred, eventually rising to 50 times their average number. The usual infective causes of abortion were ruled out by diagnostic testing; however, the results of drinking water analysis pointed to over-chlorination, with a free chlorine residual concentration of 2.11 mg/l, caused by failure of the chlorinator dispenser connected to the water supplying system seven days previously. Analysis of data on reproductive parameters indicated that the over-chlorinated drinking water directly induced an increase in the number of abortions after only two days of consumption, whereas 7-day consumption of over-chlorinated drinking water had a direct delayed effect on the increased return to oestrus in gilts and sows, and on the increased percentage of stillborn piglets (all P < 0.001). The consequential indirect delayed effect manifested itself as a reduced number of farrowings in gilts and sows, and a lower total number of piglets born (both P < 0.001). Keywords: reproductive failure, gilt, sow, watering, free chlorine residual Journal: Veterinární medicína Pages: 394-398 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2958-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2958-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0006.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2958-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F.J. Mendoza Author-Workplace-Name: Department of Animal Medicine and Surgery, University of Cordoba, Spain Author-Name: M. Lopez Author-Workplace-Name: Veterinary Teaching Hospital, University of Cordoba, Spain Author-Name: E. Diez Author-Workplace-Name: Veterinary Teaching Hospital, University of Cordoba, Spain Author-Name: A. Perez-Ecija Author-Workplace-Name: Department of Animal Medicine and Surgery, University of Cordoba, Spain Author-Name: J.C. Estepa Author-Workplace-Name: Department of Animal Medicine and Surgery, University of Cordoba, Spain Title: Uroperitoneum secondary to rupture of the urachus associated with Clostridium spp. infection in a foal: a case report Abstract: An 8-hour-old Andalusian colt was referred to the Veterinary Teaching Hospital of the University of Cordoba due to weakness, lateral recumbence, diarrhoea and absent sucking reflex. At admission the foal was obnubilated, with cold limbs, pale mucous membranes, tachycardia and diarrhoea. Laboratory results revealed increased hematocrit and total protein concentration, hyperfibrinogenemia, leukopenia, azoetemia, hypertrygliceridemia and a decreased IgG level. A diagnose of failure of passive transfer of immunoglobulins and hyperlipaemia was made. On the third day of hospitalization the foal presented painful and swollen joints, abdominal distension and fever. The ultrasonographic study at this point showed abundant hypoechogenic free fluid content in the peritoneal cavity. Abdominocentesis demonstrated a high peritoneal creatinine:serum creatinine ratio. Culture of the peritoneal fluid was Clostridium spp. positive. Necropsy revealed a tear in the internal umbilical remnant. The final diagnosis was uroperitoneum secondary to rupture of the urachus associated with a Clostridium spp. infection. New emerging aetiologies responsible for uroperitoneum, either by septic urachitis or omphalophlebitis, are emerging. Nonetheless, cases of uroperitoneum induced by Clostridium spp. infection are rare and constitute a new and important finding for equine neonatal medicine. In addition, the hyperlipaemic status in this animal could be a plausible cause leading to septicemia and subsequent uroperitoneum. Keywords: internal umbilical remnant, peritonitis, uroabdomen, clostridium, colt Journal: Veterinární medicína Pages: 399-404 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2955-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2955-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0007.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2955-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: G. Serin Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: A. Aydogan Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: R. Yaygingul Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Author-Name: R. Tunca Author-Workplace-Name: Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey Title: Uterine leiomyosarcoma in a dog: a case report Abstract: A 14-year-old, mixed breed bitch was presented for investigation of progressive abdominal distension, dyspnea and general dullness. A large abdominal mass including numerous cystic areas was visualized in abdominal radiologic examinations. Moreover, some abdominal organs were displaced from their abdominal anatomical locations. Exploratory laparotomy revealed a uterine mass, which was removed by ovariohysterectomy. Histopathology confirmed uterine leiomyosarcoma. Clinical and radiographic examination did not reveal any evidence of metastasis two months after surgery. Canine uterine leiomyosarcomas are rare tumours, grow slowly and are not regarded as highly metastatic. In this case, removal of this huge tumor by ovariohysterectomy following laparotomy was successful as metastasis did not subsequently occur. Keywords: uterine tumors, leiomyosarcoma, dog Journal: Veterinární medicína Pages: 405-408 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2959-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2959-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0008.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2959-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Skoric Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Fictum Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: V. Jekl Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: K. Hauptman Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: Z. Knotek Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Hermanova Author-Workplace-Name: St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic Title: Vaginal leiomyosarcoma in a degu (Octodon degus): a case report Abstract: A 6-year-old pet female degu (Octodon degus), in good body condition, was referred to a clinic with the presence of a large tumour in the anogenital area. The mass was bluntly dissected from the surrounding skin and muscles. The medial part of the tumour was associated with the vaginal wall which was also excised with 2 mm margins. No visible changes on the inner vaginal surface were seen. On gross examination the surface of the mass was glossy and pink-coloured; the cut surface was grey to red with greyish-white foci in a part of the mass. Histopathological examination showed a tumour composed of pleomorphic spindle to ovoid cells forming interlacing bundles and variably dense fibrous connective tissue separated by streams of neoplastic cells. Several smaller foci of coagulation necrosis were present within the tumour tissue. The neoplastic cells exhibited a high mitotic index, which ranged from six to seven mitoses per 10 high power fields. By immunohistochemical examination the positivity of neoplastic cells was demonstrated with smooth muscle actin (SMA) and vimentin, while no immunoreactivity was acquired for cytokeratins. Based on morphological features of the tumour and immunohistochemical examination a diagnosis of vaginal leiomyosarcoma was made. To our knowledge, this is the first report of a vaginal leiomyosarcoma in a degu. Keywords: mesenchymal tumour, genital tract, rodent, immunohistochemistry, smooth muscle actin Journal: Veterinární medicína Pages: 409-412 Volume: 55 Issue: 8 Year: 2010 DOI: 10.17221/2960-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2960-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201008-0009.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:8:id:2960-VETMED