Template-Type: ReDIF-Article 1.0 Author-Name: editors Author-Workplace-Name: ORIGINAL PAPERS Pavlik I, Horvathova A, Bartosova L, Babak V, Moravkova M: IS900 RFLP types of Mycobacterium avium subsp. paratuberculosis in faeces and environmental samples on four dairy cattle farms ......................................................................................................................................................................... 1 Lopatarova M, Cech S, Krontorad P, Holy L, Lalova H, Dolezel R: Conception rate after sex determination and cryopreservation of D7 bovine embryos: ................................................................................. 10 Manga-Gonzalez MY, Quiroz-Romero H, Gonzalez-Lanza C, Minambres B, Ochoa P: Strategic control of Dicrocoelium dendriticum (Digenea) egg excretion by naturally infected sheep ............. 19 Duras Gomercic M, Trbojevic Vukicevic T, Gomercic T, Galov A, Fruk T, Gomercic H: The cisterna chyli and thoracic duct in pigs (Sus scrofa domestica) ...................................................................... 30 Kaevska M, Hruska K: Analysis of publications on paratuberculosis from 1995 to 2009 with emphasis on the period from 2005 to 2009 .................................................................................................................................. 43 Krizova K, Matlova L, Horvathova A, Moravkova M, Beran V, Boisselet T, Babak V, Slana I, Pavlik I: Mycobacteria in the environment of pig farms in the Czech Republic between 2003 and 2007 .... 55 Ouwehand AC, Tiihonen K, Kettunen H, Peuranen S, Schulze H, Rautonen N: In vitro effects of essential oils on potential pathogens and beneficial members of the normal microbiota ............................ 71 Haligur A, Duzler A: Course and branch of the celiac artery in the red falcon (Buteo rufinus) ................. 79 Brtkova A, Filipova M, Drahovska H, Bujdakova H: Characterization of enterococci of animal and environmental origin using phenotypic methods and comparison with PCR based methods ................. 97 Fichtelova V, Kovarcik K: Characterization of two BHV-4 strains isolated in the Czech Republic ....... 106 Ishii M, Aoki T, Yamakawa K, Uyama T, El-khodery S, Matsui M, Miyake Y: Uterine prolapse in cows: Effect of raising the rear end on the clinical outcomes and reproductive performance ................... 113 Kolar M, Bardon J, Chroma M, Hricova K, Stosova T, Sauer P, Koukalova D: ESBL and AmpC beta-lactamase-producing Enterobacteriaceae in poultry in the Czech Republic ........................ 119 Woo D-C, Choi C-B, Nam J-W, Ryu K-N, Jahng G-H, Lee S-H, Lee D-W, Kim S-Y, Kim H-Y, Ahn K-J, Choe B-Y: Quantitative analysis of hydrocephalic ventricular alterations in Yorkshire terriers using magnetic resonance imaging ....................................................................................................................................... 125 Krametter-Froetscher R, Mason N, Roetzel J, Benetka V, Bago Z, Moestl K, Baumgartner W: Effects of Border disease virus (genotype 3) naturally transmitted by persistently infected sheep to pregnant heifers and their progeny ....................................................................................................................... 145 I #HEADER# Vet. Med. - Czech, 55, 2010 (12) Title: VOLUME 55, CONTENTS AUTHOR INDEX AUTHOR INSTITUTION INDEX LIST OF REVIEWERS Journal: Veterinární medicína Pages: I-XV Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2951-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2951-VETMED.html File-Format: text/html Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2951-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Kaevska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Hruska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Mycobacteria in water, feedstocks and food: analysis of publications Abstract: Papers on mycobacteria in food, feed and water, published between 1945 and 2010 and indexed in the database Web of Science® (Thomson Reuters) were ranked according to authors, institutions, countries and source titles. The total number of papers on mycobacteria and food and mycobacteria and water were 1486 and 1419, respectively. More than 40% of papers have been published in the last five years. In addition to publications in peer reviewed journals the archives of ProMED-mail and the Rapid Alert System for Food and Feed of the European Union were also searched. It is evident that much attention is being paid to mycobacteria in food, feed and water as they likely pose a public health risk. Keywords: food safety, public health, milk, meat, beef, cheese, fish, fruits, vegetables Journal: Veterinární medicína Pages: 571-580 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2946-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2946-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0002.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2946-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A.V. Makarevich Author-Workplace-Name: Animal Production Research Centre Nitra, Luzianky near Nitra, Slovak Republic Author-Name: E. Kubovicova Author-Workplace-Name: Animal Production Research Centre Nitra, Luzianky near Nitra, Slovak Republic Author-Name: A.V. Sirotkin Author-Workplace-Name: Animal Production Research Centre Nitra, Luzianky near Nitra, Slovak Republic Author-Name: J. Pivko Author-Workplace-Name: Animal Production Research Centre Nitra, Luzianky near Nitra, Slovak Republic Title: Demonstration of the effect of epidermal growth factor on ram sperm parameters using two fluorescent assays Abstract: ABSTRACT: The goal of this study was to examine the effect of epidermal growth factor (EGF) on sperm viability using two fluorescent techniques and to analyze the obtained results in relation to sperm motility, determined by subjective estimation. Fresh ram semen diluted in a Biladyl commercial extender was cooling stored (at 4 °C in a fridge) for four days in the presence of EGF at doses of 0, 10, 100, 200 or 400 ng/ml. Thereafter, sperm samples were analyzed for progressive motility (Motility test) and membrane integrity using two fluorescent techniques: SYBR-14/PI (Method 1) or PI/DAPI (Method 2). Application of Method 1 did not detect an effect of EGF at any concentration on sperm membrane integrity. A positive effect of EGF (200 ng/ml) on sperm membrane integrity was found using Method 2 of staining, and this result was confirmed by the sperm motility test, which demonstrated an EGF-stimulating effect (200 or 400 ng/ml) on a percentage of progressively moving spermatozoa. Strong positive correlations between Methods 1 and 2 (r = 0.785), Method 1 and Motility (r = 0.803), Method 2 and Motility (r = 0.699), as well as between both techniques taken together and the Motility test (r = 0.853) were found. Regression analysis confirmed that Method 2 was more exact than Method 1, and the results obtained with Method 2 are comparable with those of the Motility test. Dependence of the viability or motility on EGF concentrations (linear regression function) was significant only for Method 2 or the Motility test. The obtained results suggest a stimulating effect of EGF (at higher concentrations) on ram sperm functions (viability/membrane integrity and motility). Furthermore, they indicate substantial differences between two fluorescent techniques in the determination of sperm membrane integrity. Only the data obtained using PI/DAPI were confirmed by a functional Motility test. These findings suggest that the technique chosen for analysis of sperm viability can influence the conclusion concerning the effects of the treatment on sperm function. Keywords: viability, motility, membrane integrity Journal: Veterinární medicína Pages: 581-589 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2944-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2944-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0003.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2944-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N.Q. Hailat Author-Workplace-Name: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan Author-Name: W. Hananeh Author-Workplace-Name: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan Author-Name: A.S. Metekia Author-Workplace-Name: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan Author-Name: J.R. Stabel Author-Workplace-Name: USDA-ARS, National Animal Disease Centre, Ames, USA Author-Name: A. Al-Majali Author-Workplace-Name: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan Author-Name: S. Lafi Author-Workplace-Name: Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan Title: Pathology of subclinical paratuberculosis (Johne's Disease) in Awassi sheep with reference to its occurrence in Jordan Abstract: In this study, the pathological lesions and occurrence of subclinical Johne's disease in Awassi sheep is investigated using histopathological (HP) and immunohistochemical (IHC) examinations, Acid Fast Staining (AFS) of tissue sections, direct smears from tissues and culture. Histopathological examination of 202 ilea and the corresponding mesenteric lymph nodes (179) was conducted. In addition, IHC examination, using rabbit polyclonal antiserum, of 134 ilea and 123 mesenteric lymph nodes was also conducted. The occurrence of the disease was observed in 50% and 93% of the ilea examined using histopathology and IHC techniques, respectively. Fifty nine percent of lymph nodes were positive by IHC. The histopathological lesions were graded from І-IV, I being the least severe, based on the type of cellular infiltrate (lymphocytes, macrophages and epithelioid cells) and the severity of the lesions. Grades III and IV (SP) were considered positive while I and II were considered suspected. Analysis of the results also revealed that the majority of suspected cases (grades I and II) reacted positive with the IHC. Furthermore, the IHC reactions were classified into three categories depending on the number of stained cells and the intensity of the staining (I-mild, II-moderate and III-strong). Direct smears, and tissue sections obtained from the ilea and stained with AFS revealed that out of 202 tissue samples, 53 (26%) and 22 (11%) were positive, respectively. Results of the culture revealed that 22 (11%) out of 202 were positive. These results showed that the occurrence of paratuberculosis (Johne's disease) in Awassi sheep is very high in Jordan and needs further study in order to develop rational methods of control effective for the Jordanian sheep population. Keywords: sheep, paratuberculosis, histopathology, acid fast stain, ileum, immunohistochemistry Journal: Veterinární medicína Pages: 590-602 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2947-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2947-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0004.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2947-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: J. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: Y. Cho Author-Workplace-Name: Radiologic Technology, Daegu Health College, Daegu, Republic of Korea Author-Name: M. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: N. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: K. Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Title: Three-dimensional computed tomographic volume rendering imaging as a teaching tool in veterinary radiology instruction Abstract: The educational value of three-dimensional computed tomography (3D CT) volume rendering imaging was compared to conventional plain radiographic instruction in a veterinary radiology class. Veterinary radiology is an important subject in veterinary medicine and has been well-recognized as a primary diagnostic method. Many junior and senior students have difficulty interpreting two dimensional radiographs that depict three-dimensional organs. A total of 158 junior veterinary students with knowledge of anatomy, pathology, physiology, and other basic subjects were divided into two groups; Group 1 (n = 45) received conventional radiographic instruction using normal and representative abnormal canine thoracic and abdominal radiographs followed by repetition of the same one week later, while Group 2 (n = 113) received plain radiograph instruction as in Group 1 followed by volume-rendered 3D CT images from the same canine patient one week later. The evaluations were performed at the end of each instruction. In Group 1, the majority did not understand the radiographic signs and no significant improvement was observed. In Group 2, 13% and 20% of the students learned only from radiographs, and understood the thoracic and abdominal radiographic alterations, respectively. After studying the 3D CT images, more than 94% of the students deduced the reasons for the radiographic alterations on the radiographs (P < 0.001). These results strongly suggest that 3D CT imaging is an effective tool for teaching radiographic anatomy to veterinary medical students. Keywords: three-dimensional CT, teaching, veterinary radiology, dog Journal: Veterinární medicína Pages: 603-609 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2950-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2950-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0005.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2950-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Vrublova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: J. Vostalova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: J. Ehrmann Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: I. Palikova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: J. Vrbkova Author-Workplace-Name: Faculty of Science, Palacky University, Olomouc, Czech Republic Author-Name: J. Vacek Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: N. Cibicek Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: R. Vecera Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: J. Ulrichova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: V. Simanek Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Title: The phytogenic feed additive Sangrovit modulates dextran sulfate sodium-induced colitis in rats Abstract: The alkaloids of Macleaya cordata (Papaveracae) are active components of the phytogenic feed additive Sangrovit. The present study was conducted to evaluate the effects of Sangrovit on dextran sulfate sodium (DSS)-induced colitis using rats as an experimental model. Thirty-five male rats were randomly assigned to a control group (Group 1, n = 5), a Sangrovit group (Group 2, n = 20) and a DSS group (Group 3, n = 10). Group 1 received standard diet and tap water for 14 days. Group 2 received 500 ppm Sangrovit in their feed for 14 days and in the second week 5% DSS was added to the tap water. The animals in Group 3 were fed for seven days with standard diet and tap water and for the next seven days with standard diet and 5% DSS added to their tap water. The rats were sacrificed on day 14 and the following parameters were measured: disease activity (body and organ weight, colon length, presence of blood in stool), colon myeloperoxidase activity, expression of colon cyclooxygenase-2 (cox-2), hematological parameters, histological colitis score and selected parameters of oxidative stress. The animals treated with DSS for seven days (Groups 2 and 3) showed increases in liver and cecum weight, leukocyte count and colon shortening, decreases in hemoglobin and hematocrit associated with hematochezia. In comparison with Group 3 where DSS caused mucosal edema, cellular infiltration and epithelial disruption, the Sangrovit group showed less severe damage to the colon mucosa and decreased histological colitis scores. The Sangrovit group also showed diminished expression of DSS-induced COX-2, significantly mitigated myeloperoxidase activity in colon tissue and level of reduced glutathione in erythrocytes. In conclusion, some parameters of DSS-induced colitis were modulated by 500 ppm Sangrovit added to feed. Keywords: Macleaya cordata, Papaveraceae, benzo[c]phenanthridines, protopines, feed additive, dextran sulfate sodium-induced colitis, cyclooxygenase-2, myeloperoxidase Journal: Veterinární medicína Pages: 610-618 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2945-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2945-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0006.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2945-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Yaman Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: A.S. Durmus Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: S. Ceribasi Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Author-Name: M. Yaman Author-Workplace-Name: Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey Title: Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats Abstract: This experiment was conducted in order to compare the effects of Nigella sativa (NS) and silver sulfadiazine (SSD) cream on healing of burn wounds in rats. Fifty four adult, male Wistar-albino rats were divided into three groups of equal numbers. A burn was generated on the backs of all the rats. The burned areas in the first, second and third groups were covered with daily cold cream (control), SSD cream and NS cream (50% NS oil + 50% cold cream), respectively. Four, nine, and 14 days later, the rats were sacrificed and the burned skin tissue samples were collected for histopathological examinations. Histopatological evaluations on the 4th, 9th and 14th days showed burn healing to be better in the NS and SSD groups with respect to the control group. Wound healing was significantly different among the groups at 4th, 9th and 14th days (P < 0.001). In conclusion, application of NS and SSD cream are effective in healing burn related skin wounds in the rat model. Keywords: Nigella sativa, silver sulfadiazine, burn, rat Journal: Veterinární medicína Pages: 619-624 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2948-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2948-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0007.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2948-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Pribylova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Slana Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Lamka Author-Workplace-Name: Charles University in Prague, Faculty of Pharmacy, Hradec Kralove, Czech Republic Author-Name: V. Babak Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: K. Hruska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: I. Pavlik Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report Abstract: The aim of this study was to monitor over two years a farmed mouflon herd for the presence and persistence of Mycobacterium avium subsp. paratuberculosis (MAP) using an IS900 real-time PCR method. This study followed the previous monitoring of the herd using a cultivation method which showed only a minimal infection load among the animals. Although no mouflon showed clinical symptoms, 35.7% and 80% of ewes were IS900-positive in 2008 and 2009, respectively. In seven out of 21 adult ewes, the presence of the IS900 sequence was determined in 2008 as well as in 2009. Between the first and second sampling, twenty-three mouflon lambs born and kept with the ewes were examined. Almost one third of them (30.4%) were proven to have the MAP sequence in their faeces. Also, 75% environmental samples from the mouflon farm showed positivity. Infected animals without clinical symptoms which low sensitivity cultivation does not detect represent a source of infection for other animals. Therefore, real-time PCR has a crucial role in paratuberculosis control programs, especially in control of the disease by the culling of infected animals. Keywords: MAP, Johne's disease, faeces, environment, qPCR Journal: Veterinární medicína Pages: 625-630 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2943-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2943-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0008.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2943-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Kumar Author-Workplace-Name: Indian Veterinary Research Institute, Bareilly, India Author-Name: R.V.S. Pawaiya Author-Workplace-Name: Indian Veterinary Research Institute, Bareilly, India Author-Name: B.P. Madhu Author-Workplace-Name: Indian Veterinary Research Institute, Bareilly, India Title: Multiple mammary tumours in a bitch: analysis of mitotic index, AgNOR count and c-erbB2 expression status: a case report Abstract: This case report of multiple mammary tumours in a Spitz bitch revealed the presence of three tumour masses, T-1, T-2 and T-3, of variable size, shape and texture on different mammary glands. Histopathological examination revealed these tumours to be of different histological type (T-1 - fibrosarcoma, T-2 - malignant mixed mammary tumour and T-3 - papillary adenocarcinoma). The mitotic index and AgNOR count was high in all three tumours indicative of a higher proliferation status of neoplastic cells while T-2 and T-3 showed overexpression of the c-erbB2 oncoprotein. Keywords: multiple mammary tumours, bitch, c-erbB2, AgNOR Journal: Veterinární medicína Pages: 631-635 Volume: 55 Issue: 12 Year: 2010 DOI: 10.17221/2949-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/2949-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201012-0009.txt Handle: RePEc:caa:jnlvet:v:55:y:2010:i:12:id:2949-VETMED