Template-Type: ReDIF-Article 1.0 Author-Name: R. Vecera Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: A. Zacharova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: J. Orolin Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: N. Skottova Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Author-Name: P. Anzenbacher Author-Workplace-Name: Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic Title: The effect of silymarin on expression of selected ABC transporters in the rat Abstract: Silymarin (standardized extract from the seeds of the Silybum marianum) has been used in the supportive therapy of liver diseases and its cytoprotective activity is believed to be based on antioxidant properties. Our previous works showed hypolipidemic effects of silymarin. The ATP-binding cassette (ABC) transporters G5 and G8 play a major role in biliary cholesterol secretion. The ABCA1 transporter plays a significant role in movement of cellular cholesterol to high density lipoproteins. We investigated the possibility that silymarin affects the regulation of lipid metabolism via selected ABC transporters using rats fed a high-cholesterol diet with silymarin. The major finding in this study is that silymarin up-regulated mRNA of ABC transporters (G5, G8 and A1). This result suggests that silymarin positively affects the plasma lipoprotein profile via up-regulation of ABC transporters involved in lipid metabolism. Furthermore, this study shows for the first time that silymarin up-regulates the expression of these ABC transporters at the mRNA level. Keywords: ABC transporter, lipid metabolism, mRNA, rat, silymarin Journal: Veterinární medicína Pages: 59-62 Volume: 56 Issue: 2 Year: 2011 DOI: 10.17221/1579-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1579-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201102-0001.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:2:id:1579-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Pavlata Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: M. Chomat Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: A. Pechova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Misurova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Dvorak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Impact of long-term supplementation of zinc and selenium on their content in blood and hair in goats Abstract: This paper evaluates the impact of long-term supplementation of different forms of zinc (Zn) and selenium (Se) on the content of these substances in the blood and hair of goats. Two analogous supplementation experiments were performed. 37 goats divided into four groups were used in the first trial with the Zn supplementation. Group A (n = 10) was a control group (with no Zn administered). A further three groups (B, C, D) were supplemented with Zn in various forms. Group B (n = 9) with zinc oxide, Group C (n = 9) with zinc lactate and Group D (n = 9) with zinc chelate. The second trial with Se supplementation was carried out on 20 goats divided into four groups. Group E (n = 5) was a control group. The other three groups were administered Se. Group F (n = 5) was supplied with a selenium lactate-protein complex, Group G (n = 5) with sodium selenite and Group H (n = 5) with selenium yeast. Three months later blood and hair samples were taken from all animals and Zn and Se concentrations were determined in whole blood, plasma, and hair. Glutathione peroxidase (GSH-Px) activity was determined in the Se supplementation trial group. At the end of the trial the Zn concentrations in plasma and whole blood were without major differences between the groups. The plasma concentration of Zn did not increase from the initial value at the start of the trial. In hair the average concentration of Zn was 95.2-100.0 mg/kgin all groups. No conclusive relation was confirmed between the values of Zn in hair and its concentration in blood. The Se concentration in whole blood (µg/l) at the end of trial in supplemented groups (F - 188.8 ± 24.6; G - 197.2 ± 10.9; H - 190.1 ± 26.3) was significantly higher (P < 0.01) than in the control group (E - 103.1 ± 23.5). Similarly, the activity of GSH-Px (µkat/l) was significantly higher in all supplemented groups (F - 872.3 ± 94.8; G - 659.5 ± 176.4; H - 839.8 ± 150.8) than in the control group (E - 379.1 ± 63.5). Se content in hair (µg/kg) was higher also in all trial groups (F - 242.3 ± 41.5; G - 200.5 ± 46.9; H - 270.0 ± 106.8) than in the control group (E - 174.7 ± 38.0). However, it was significantly (P < 0.05) higher only in Group F. A conclusive correlation was identified between the Se concentration in whole blood and its content in hair (r = 0.54; P < 0.05; n = 20). Based on the results it can be concluded that none of the supplemented forms of Zn increased its concentration in blood, plasma and hair. On the other hand, the administration of Se led to an increase in the Se concentration in blood, increased the activity of GSH-Px in whole blood and the Se content in hair. Based on the proven correlation and regression relation between the Se concentration in blood and its content in hair, hair can be considered as a suitable material for the diagnosis of long-term Se status in goats. Goats with sufficient Se status are those that have more than 160 µg/kg of Se in hair dry weight. Keywords: trace element, glutathione peroxidase, organic selenium, inorganic selenium, organic zinc, inorganic zinc, ruminants, metabolism Journal: Veterinární medicína Pages: 63-74 Volume: 56 Issue: 2 Year: 2011 DOI: 10.17221/1581-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1581-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201102-0002.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:2:id:1581-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Pavlata Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Misurova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: A. Pechova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Dvorak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: The effect of inorganic and organically bound forms of selenium on glutathione peroxidase activity in the blood of goats Abstract: The goal of the experiment was to compare the effect of supplementation of inorganic and the new organically bound (lactate-protein selenium complex) form of selenium (Se) in feed for goats. The 31 goats were split into three groups: control (C) without Se supplementation, AN group administered sodium selenite, ORG group administered lactate-protein selenium complex (Selene Chelate, Agrobac, Czech Republic) produced by cultivation of Lactobacillus acidophilus on a substrate containing natrium selenite. The total Se intake in goats was 0.15 mg in group C, and 0.43 mg in the groups AN and ORG. The effect of Se supplementation was assessed based on the determination of Se concentration and the activity of glutathione peroxidase (GSH-Px) in whole blood. Samples were taken before the beginning of Se supplementation, 14 and 30 days after the start of supplementation, and then two and three months after the beginning of supplementation. Average Se concentrations in the blood of goats in individual groups (C, AN, ORG) before the start of supplementation were 109.6 ± 34.3, 117.5 ± 34.7, and 105.4 ± 43.6 μg/l respectively, and the activity of GSH-Px in whole blood was 745.3 ± 289.2, 810.7 ± 280.4, and 791.0 ± 398.1 μkat/l respectively. While in group C goats neither the Se concentration nor the GSH-Px activity changed substantially during the experiment, in the goats in the experimental groups there was a statistically significant increase (P < 0.01) in both Se concentrations and the GSH-Px activities. At the end of the experiment Se concentrations in the blood of AN and ORG groups amounted to 168.5 ± 12.2 and 168.8 ± 26.8 μg/l. The GSH-Px activities in goats supplemented with Se also increased significantly over the course of the experiment (at the end of the experiment it was 1178.0 ± 127.3 in the AN group and 1030.1 ± 152.3 μkat/l in the ORG group), and the difference between the groups was significant (P = 0.038). Regarding the dynamics of GSH-Px activity changes during the monitored period, a markedly quicker increase in GSH-Px activity was recorded in the AN group - one month after the beginning of Se supplementation, compared to three months after the beginning of Se supplementation in the ORG group. The results thus show that the effects of supplementation with selenite and the lactate-protein selenium complex are similar with regard to Se status, but that the increase in GSH-Px activity occurred much faster with selenite, which therefore appears to be a more biologically available form of selenium for creation of biologically active selenoproteins. Keywords: selenium metabolism, ruminants, selenoproteins, organic selenium, blood selenium Journal: Veterinární medicína Pages: 75-81 Volume: 56 Issue: 2 Year: 2011 DOI: 10.17221/1576-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1576-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201102-0003.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:2:id:1576-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: N.K. Sood Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India Author-Name: L.D. Singla Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India Author-Name: R.S. Singh Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India Author-Name: S.K. Uppal Author-Workplace-Name: College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India Title: Association of Trypanosoma theileri with peritonitis in a pregnant cross-bred cow: a case report Abstract: This clinical case report deals with a very rare case of the detection of Trypanosoma theileri in the peritoneal fluid of a seven-year-old eight month pregnant cross-bred cow in fourth parity showing frank peritonitis. Peritoneal tab cytology revealed chronic active peritonitis and the presence of polymorphic T. theileri organisms. The parasite was also occasionally detected in blood smears. The protozoan, considered by and large non-pathogenic, has previously been detected in aberrant body sites, other than the peritoneal cavity. A perusal of the available literature indicates that this is the first report of this large stercorarrian trypanosome associated with peritonitis, which was characterized by infiltration of mononuclear cells and neutrophils, as well as a significant number of intact and degranulating eosinophils, not usually seen in protozoan infections. Keywords: cross-bred cow, cytology, peritonitis, Trypanosoma theileri Journal: Veterinární medicína Pages: 82-84 Volume: 56 Issue: 2 Year: 2011 DOI: 10.17221/1580-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1580-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201102-0004.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:2:id:1580-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: C.F. Agudelo Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: P. Schanilec Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: K. Kybicova Author-Workplace-Name: National Institute of Public Health, National Reference Laboratory of Lyme Borreliosis, Prague, Czech Republic Author-Name: P. Kohout Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Cardiac manifestations of borreliosis in a dog: a case report Abstract: In Europe Lyme disease is caused by the spirochete Borrelia burgdorferi sensu lato. It presents with a variety of clinical manifestations including heart disease and problems of the nervous system, skin and joints. Lyme carditis occurs in 4-10% of infected humans and is characterized by a plethora of cardiovascular syndromes such as arrhythmia, myocarditis, and pericarditis among others. This article is a case report of a serologically positive (B. burgdorferi s.l.) dog, in which the antibody dynamics correlated with clinical, electrocardiographic, and echocardiographic findings of heart disease resembling human Lyme-induced dilated cardiomyopathy (DCM). Keywords: borreliosis, atrial fibrillation, serology, cardiomegaly, dogs Journal: Veterinární medicína Pages: 85-92 Volume: 56 Issue: 2 Year: 2011 DOI: 10.17221/1577-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1577-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201102-0005.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:2:id:1577-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Zafra Author-Workplace-Name: Faculty of Veterinary Medicine, University of Cordoba, Spain Author-Name: M.J. Bautista Author-Workplace-Name: Faculty of Veterinary Medicine, University of Cordoba, Spain Author-Name: J. Perez Author-Workplace-Name: Faculty of Veterinary Medicine, University of Cordoba, Spain Author-Name: P.J. Ginel Author-Workplace-Name: Faculty of Veterinary Medicine, University of Cordoba, Spain Author-Name: R. Lucena Author-Workplace-Name: Faculty of Veterinary Medicine, University of Cordoba, Spain Title: Hepatopancreatic ganglioneuroma in a young dog: a case report Abstract: The clinical, pathological and immunohistochemical features of a hepatobiliary and pancreatic ganglioneuroma in a seven-month-old dog are described. The dog presented progressive weight loss, anorexia and abdominal pain. At laparoscopic examination numerous whitish nodules ranging from 0.5 to 1 cm in diameter were found in the peripancreatic tissues, the bile duct system and perihepatic tissues. Due to poor prognosis the dog was euthanized and necropsy was conducted. Tumour nodules were not noted in the other examined abdominal organs. Microscopically, the nodules were composed of large, well differentiated neurons embedded in an abundant stroma of nerve fibres and connective fibrous tissue. Through immunohistochemistry analysis, neurons were found to express neurofilaments and did not express S-100 protein. The histopathological and immunohistochemical features were consistent with a diagnosis of ganglioneuroma. This case is atypical as the majority of reported canine ganglioneuromas involve the gastrointestinal tract. The early age of the dog is also considered to be atypical for this tumour. Keywords: ganglioneuroma, dog, immunohistochemistry, liver, pancreas Journal: Veterinární medicína Pages: 93-96 Volume: 56 Issue: 2 Year: 2011 DOI: 10.17221/1578-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1578-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201102-0006.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:2:id:1578-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: F. Hayati Author-Workplace-Name: School of Veterinary Medicine, Tehran University, Tehran, Iran Author-Name: A. Lakzian Author-Workplace-Name: School of Veterinary Medicine, Tehran University, Tehran, Iran Author-Name: E. Shariati Author-Workplace-Name: School of Veterinary Medicine, Tehran University, Tehran, Iran Author-Name: J. Bakhtiari Author-Workplace-Name: School of Veterinary Medicine, Tehran University, Tehran, Iran Author-Name: N. Sayyah Author-Workplace-Name: School of Veterinary Medicine, Tehran University, Tehran, Iran Author-Name: M. Masoudifard Author-Workplace-Name: School of Veterinary Medicine, Tehran University, Tehran, Iran Author-Name: T. Vakili Author-Workplace-Name: Private Practitioner, Mashhad, Iran Title: Surgical removal of a ventricular foreign body from a common myna (Acridotheres tristis): a case report Abstract: A common myna (Acridotheres tristis) was presented to Veterinary Teaching Hospital, School of Veterinary Medicine, Tehran University after swallowing a needle. Radiography and physical examination revealed a metal foreign body within the body with its distal end in the ventriculus. Surgical removal by ventriculotomy under general anesthesia was attempted. Six months of follow up study revealed no complications. Keywords: common myna, Acridotheres tristis, foreign body, ventriculotomy Journal: Veterinární medicína Pages: 97-100 Volume: 56 Issue: 2 Year: 2011 DOI: 10.17221/1582-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1582-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201102-0007.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:2:id:1582-VETMED