Template-Type: ReDIF-Article 1.0 Author-Name: B. Kempisty Author-Workplace-Name: Poznan University of Medical Sciences, Poznan, Poland Author-Name: D. Bukowska Author-Workplace-Name: Poznan University of Life Sciences, Poznan, Poland Author-Name: H. Piotrowska Author-Workplace-Name: Poznan University of Medical Sciences, Poznan, Poland Author-Name: P. Zawierucha Author-Workplace-Name: Poznan University of Medical Sciences, Poznan, Poland Author-Name: P. Sniadek Author-Workplace-Name: Wroclaw University of Technology, Wroclaw, Poland Author-Name: R. Walczak Author-Workplace-Name: Wroclaw University of Technology, Wroclaw, Poland Author-Name: J. Dziuban Author-Workplace-Name: Wroclaw University of Technology, Wroclaw, Poland Author-Name: P. Antosik Author-Workplace-Name: Poznan University of Life Sciences, Poznan, Poland Author-Name: J. Jaskowski Author-Workplace-Name: Poznan University of Life Sciences, Poznan, Poland Author-Name: K.P. Brussow Author-Workplace-Name: Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany Author-Name: M. Nowicki Author-Workplace-Name: Poznan University of Medical Sciences, Poznan, Poland Author-Name: M. Zabel Author-Workplace-Name: Poznan University of Medical Sciences, Poznan, Poland Title: Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review Abstract: Maturation of oocytes is the most important stage, which determines the subsequent successful fertilization, zygote formation, attainment of blastocyst stage, normal embryo growth and development, as well as appropriate implantation. Since in some species of mammals the efficiency of IVM maturation is still very low, many studies have focused on new combinations of media supplements. In some species of mammals, including pigs, mice, bovines, goats and dogs, EGF, BSA, and progesterone are successfully used as an enhancer of the IVC system. Application of the Lab-on-Chip system in reproductive biology opens new possibilities for the development of techniques available for the assessment of the developmental competency or potency manifested by mammalian oocytes and embryos. In most cases, the Lab-on-Chip system was used in mice. However, an increasing number of examples are available in which the system has been applied to evaluate porcine and bovine gametes and embryos. In this article, the role of EGF, BSA and progesterone is described in relation to maturation efficiency of mammalian oocytes. Moreover, the possibilities of using microfluidics (Lab-on-Chip) for the detection of oocyte developmental competency are also shown. Keywords: microfluidics, Lab-on-chip, oocyte maturation, media supplementation Journal: Veterinární medicína Pages: 367-378 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1552-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1552-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0001.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1552-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.S. Rahman Author-Workplace-Name: Bangladesh Agricultural University, Mymensingh, Bangladesh Author-Name: M.O. Faruk Author-Workplace-Name: Bangladesh Agricultural University, Mymensingh, Bangladesh Author-Name: M. Her Author-Workplace-Name: Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang, Gyeonggi-do, South Korea Author-Name: J.Y. Kim Author-Workplace-Name: Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang, Gyeonggi-do, South Korea Author-Name: S.I. Kang Author-Workplace-Name: Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang, Gyeonggi-do, South Korea Author-Name: S.C. Jung Author-Workplace-Name: Animal, Plant and Fisheries Quarantine and Inspection Agency, Ministry of Food, Agriculture, Forestry and Fisheries, Anyang, Gyeonggi-do, South Korea Title: Prevalence of brucellosis in ruminants in Bangladesh Abstract: The prevalence of brucellosis was determined in the ruminants (buffaloes, cattle, sheep and goats) of five different districts viz. Bagerhat, Bogra, Gaibangha, Mymensingh and Sirajgonj of Bangladesh. A total of 550 sera samples of 105 buffaloes, 188 cattle, 127 goats and 130 sheep were screened by RBT and were further confirmed with I-ELISA. A structured questionnaire was used to collect epidemiological information on the animals. The overall serological prevalence derived from the samples was 2.87% in buffaloes, 2.66% in cattle, 3.15% in goats, and 2.31% in sheep. The prevalence was relatively higher in females than that in males in cattle, goats and sheep but, an insignificantly higher prevalence was observed in males than that in females in the case of buffalo. A significant association was found between abortion or age and occurrence of brucellosis (P < 0.01). The results of the study provide (a) a comparison of the prevalence of brucellosis in different livestock species in Bangladesh, (b) constitute baseline data for further study of Brucella infections, and (c) are a starting point for the control of brucellosis. Keywords: brucellosis, ruminants, Bangladesh, seroprevalence Journal: Veterinární medicína Pages: 379-385 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1555-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1555-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0002.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1555-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: E. Atil Author-Workplace-Name: Pendik Veterinary Control and Research Institute, Istanbul, Turkey Author-Name: H.B. Ertas Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Author-Name: G. Ozbey Author-Workplace-Name: Vocational School of Health Services, Firat University, Elazig, Turkey Title: Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples Abstract: A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from February 2007 to January 2008). These isolates were identified by conventional and PCR techniques as belonging to L. monocytogenes (17.4%), to Listeria innocua (39.1%), to Listeria seeligeri 17.4%), to Listeria grayi (15.2%) and to Listeria welshimeri (11%). No Listeria ivanovii were isolated from any of the samples. Listeria spp. were not isolated from cheese and bulk tank swabs. With regard to seasonal variations most Listeria spp. were isolated in the spring and winter seasons. The eight L. monocytogenes isolates were characterized by PCR-RFLP with AluI and Tsp509I. RFLP typing of the isolates revealed two different profiles with both restriction enzymes. Four and six different profiles were produced in the examination of L. monocytogenes isolates with RAPD analysis with HLWL74 and HLWL85 primers, respectively. This is the first report on the genotyping of L. monocytogenes isolates from various sources in Turkey. This study has highlighted the need for improved control and epidemiologic strategies to prevent the transmission of Listeria spp. to animals and humans. Keywords: Listeria, seasonal variation, PCR-RFLP, RAPD Journal: Veterinární medicína Pages: 386-394 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1551-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1551-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0003.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1551-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Rahimi Author-Workplace-Name: Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran Title: Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran Abstract: Avian metapneumovirus causes an acute highly contagious upper respiratory tract infection primarily of turkeys and chickens. The disease can cause significant economic losses in turkey and chicken flocks, particularly when exacerbated by secondary pathogens. The purpose of this study was to determine the prevalence of avian metapneumovirus antibodies in broiler and broiler breeder flocks in Kermanshah province, west of Iran. All the flocks had not been vaccinated against avian metapneumovirus. The province were divided into four geographic areas; southwest, southeast, northwest, and northeast. Flocks in each area, and 14-15 birds in each flock, were randomly sampled. The blood samples were taken regardless of the presence of any signs of respiratory or any other clinical disease in the flocks. A total of 435 blood samples were collected from 30 commercial chicken flocks (24 broiler flocks, aged between six and eight weeks, and six broiler breeder flocks, aged between 56 and 72 weeks). The presence of antibodies against avian metapneumovirus in each serum sample was tested twice by enzyme-linked immunosorbent assay using a commercial kit which was able to determine antibodies against A, B and C subtypes of avian metapneumovirus. Out of 347 serum samples obtained from broiler chickens, 167 (48.1%) were positive to avian metapneumovirus antibodies, which represented 20 (83.3%) of 24 examined broiler flocks. Out of 88 samples obtained from broiler breeder chickens, 82 (93.2%) were positive to avian metapneumovirus antibodies, which belonged to six (100%) of examined broiler breeder flocks. Detection of anti-avian metapneumovirus antibodies among broiler breeder (100%) was higher than broiler (83.3%) flocks. A higher rate of seropositivity (83.3% of samples and 100% of broiler flocks) was observed in northwest. The results of this study may indicate the possible involvement of avian metapneumovirus in the respiratory disease we are seeing in chickens in Iran. Its prevalence has to be investigated in other parts of Iran. Future work may and should include the use of molecular methods and isolation of the virus. Isolation of avian metapneumovirus will allow the possibility of making autogenous vaccines. Keywords: avian metapneumovirus, seroprevalence, avian pneumovirus, turkey rhinotracheitis, chicken Journal: Veterinární medicína Pages: 395-399 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1554-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1554-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0004.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1554-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H.H. Ari Author-Workplace-Name: Veterinary Faculty, University of Yuzuncu Yil, Van, Turkey Author-Name: Z. Soyguder Author-Workplace-Name: Veterinary Faculty, University of Yuzuncu Yil, Van, Turkey Author-Name: S. Cinaroglu Author-Workplace-Name: Veterinary Faculty, University of Yuzuncu Yil, Van, Turkey Author-Name: S. Sefergil Author-Workplace-Name: Faculty of Veterinary, University of Manas, Bishkek, Kyrgyzstan Title: A macroanatomic study on the facial vein and its branches in the Van cat Abstract: In this study, five adult Van cats of both sexes, which were obtained from the Van Cat Research Centre, were used as materials. After the washing of the veins of the cats with saline via an external jugular vein, latex was injected via the same vessel into the vein system. Then, materials were fixed and after solidification of the latex, the veins were dissected, identified and presented in images. The majority of the venous drainage of the face is done by the facial vein, which is the direct continuation of the linguofacial vein in the intermandibular region, and its branches. In the Van cat, the facial vein gives off the inferior labial vein, the deep facial vein, the angular vein of the mouth and the masseteric branch on the lateral surface of the face. The facial vein then gives off the medial inferior palpebral vein, the superior labial vein and the lateral nasal vein at the level of the levatory nasolabial muscle and the angular vein of the eye and the dorsal nasal vein at the level of medial angle of the eye. The presence of a masseteric branch which is given by the facial vein and the ramifying of the deep facial vein into the descending palatine vein, the anastomotic branch of the superficial temporal vein and the external ophthalmic vein which is given by the deep facial vein in the Van cat demonstrates that there are differences in the presence, ramification and distribution either of the facial vein or of its branches as compared to domestic cats. Keywords: Van cat, facial vein, anatomy Journal: Veterinární medicína Pages: 400-404 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1557-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1557-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0005.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1557-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Aydin Author-Workplace-Name: Faculty of Veterinary Medicine, Firat University, Turkey Title: The spinal nerves that constitute the brachial plexus in the red squirrel (Sciurus vulgaris) Abstract: The brachial plexus in adult red squirrels (Sciurus vulgaris) was found to be formed by the rami ventralis of C5, C6, C7, C8. A thin branch of C5 and C6 constituted the cranial trunk, and the caudal trunk was formed completely by the rami ventralis of C7 and C8. Thus, in squirrels, the spinal nerves which form the brachial plexus and the joining of these spinal nerves to each other differ from other rodents and mammals. Keywords: spinal nerves, rami ventralis, brachial plexus, red squirrels Journal: Veterinární medicína Pages: 405-408 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1556-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1556-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0006.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1556-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: L. Dufkova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: P. Kulich Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: J. Prodelalova Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report Abstract: Porcine sapoviruses, members of the family Caliciviridae, have been considered as an aetiological agent of gastroenteritis in pigs. In this study, we analysed 251 faecal samples obtained from 3 to 90 day-old diarrhoeic pigs in the Czech Republic between January 2005 and June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. The presence of sapovirus (SaV) was confirmed by an RT-PCR assay with primers specific for the sapoviral RNA polymerase and capsid genes. Phylogenetic analysis based on a partial sequence of the RNA polymerase gene placed the new Czech isolate into the GVII genogroup of porcine sapoviruses; however, analysis of a portion of the capsid gene sequence classified the isolate as GIII of the genus Sapovirus. These contradictory findings indicate that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic Keywords: calicivirus, RT-PCR, phylogenetic analysis, genogroup, enteritis Journal: Veterinární medicína Pages: 409-415 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1558-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1558-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0007.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1558-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Tamburro Author-Workplace-Name: Depatment of Veterinary Medical Sciences, University of Bologna, Italy Author-Name: M. Pietra Author-Workplace-Name: Depatment of Veterinary Medical Sciences, University of Bologna, Italy Author-Name: G. Militerno Author-Workplace-Name: Depatment of Veterinary Medical Sciences, University of Bologna, Italy Author-Name: A. Diana Author-Workplace-Name: Depatment of Veterinary Medical Sciences, University of Bologna, Italy Author-Name: A. Spadari Author-Workplace-Name: Depatment of Veterinary Medical Sciences, University of Bologna, Italy Author-Name: S. Valentini Author-Workplace-Name: Depatment of Veterinary Medical Sciences, University of Bologna, Italy Title: Left cranial lung torsion in a Bernese Mountain dog: a case report Abstract: Lung lobe torsion (LLT) is an uncommon pathology in small animal practice. In large breed dogs LLT effects are localized mainly to the middle lobe of the right lung. This report describes a case of left cranial lung torsion in a Bernese Mountain dog: the patient was referred with a two-day history of asthenia, anorexia, dyspnea and haemoptysis. No trauma was reported. Physical examination showed the presence of bilateral pleural effusion confirmed by radiography and ultrasonography. Broncoscopy revealed that the left cranial lobe appeared to be compressed laterally with complete occlusion of the lumen. Thoracoscopy was performed to exclude other pathologies of the pleural space. The pathological lobe was removed. Histological examination revealed aspects consistent with lung lobe torsion. Clinical follow up carried out after three months showed a normal clinical course. Keywords: lung, lobe, torsion, Bernese, dog Journal: Veterinární medicína Pages: 416-422 Volume: 56 Issue: 8 Year: 2011 DOI: 10.17221/1553-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/1553-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201108-0008.txt Handle: RePEc:caa:jnlvet:v:56:y:2011:i:8:id:1553-VETMED