Template-Type: ReDIF-Article 1.0 Author-Name: K. Hruska Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Author-Name: M. Franek Author-Workplace-Name: Veterinary Research Institute, Brno, Czech Republic Title: Sulfonamides in the environment: a review and a case report Abstract: Sulfonamides are widely used in treatment of animals and humans but pose a risk as environmental pollutants. An analysis of 1588 publications focused on sulfonamides is presented here. The review deals with environmental pollution with sulfonamides, described in papers indexed in the database Web of Science from 1938 to 2011. More in depth details are presented regarding publication activity during the last ten years in which 1255 papers have been published by authors from 1100 institutions. Papers, published during the last three years and mainly in 2011, are listed in comprehensive tables, sorted according to five criteria: reviews, contaminated niches, risk of contamination, sulfonamide transformation and methods of analysis. Key words and shortened abstracts direct the reader to the topics of interest. Hyperlinks to full papers, published in open access journals, are another aid in knowledge dissemination. This design of the review article allows easy navigation through vast amounts of information. Finally, a case report illustrates experiences from the author's laboratory with sulfamethazine determination in pig slurry by ELISA. The reported results highlight the need for updating the legal directives for environmental protection. Keywords: sulfonamides, sulfamethazine, sulfamethoxazole, environmental protection, bacterial resistance, pig slurry, novel review design Journal: Veterinární medicína Pages: 1-35 Volume: 57 Issue: 1 Year: 2012 DOI: 10.17221/4969-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/4969-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201201-0001.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:1:id:4969-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: R. Dolezel Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: K. Ruzickova Author-Workplace-Name: Private Veterinary Practices, Velke Nemcice, Czech Republic Author-Name: G. Maceckova Author-Workplace-Name: Baby Horse Center, Beroun, Czech Republic Title: Growth of the dominant follicle and endometrial folding after administration of hCG in mares during oestrus Abstract: The purpose of the trial was evaluation of follicular growth and endometrial folding in mares after human chorionic gonadotropin (hCG) treatment in comparison with untreated mares during oestrus. In addition, the influence of follicle size at the time of hCG treatment on these parameters was evaluated. HCG (3000 IU) was administered intravenously in 17 mares bearing dominant follicles 35-40 mm in diameter (Group A) and in 13 mares with larger follicles (Group B). Ten mares with follicles ≥ 35 mm were untreated (Group C). Ultrasonographical examination of the mares continued in 6 h intervals until ovulation. Growth of the dominant follicle was faster in Group A than in Groups B and C (1.3 vs. 0.3 and 0.7 mm/6h, P < 0.05) but diameters of the preovulatory follicles were similar - 44, 48 and 44 mm in Groups A, B and C, respectively. Similarly, reduction of endometrial folding (on a three point scale) during observation was higher in Group A than in B and C (2.1 vs. 1.2 and 1.8, A : B P < 0.05) but endometrial folding values in the term before ovulation were not different (0.6, 0.9 and 0.6 in Groups A, B and C). A positive correlation between the speed of follicular growth and reduction of endometrial folding was found (rs - 0.479, P = 0.003). Irregularity in follicle shape (the difference between the longest axis and its perpendicular axis) at the beginning of observation (3.3, 4.0 and 3.2 mm) was lower than before ovulation (7.4, 10.4 and 9.2 mm) in all groups (P < 0.01). The interval from the beginning of observation until ovulation was significantly shorter in Groups A and B versus C (37 and 31 vs. 103 h, P < 0.01). The results show that growth of dominant follicles after hCG is influenced by the size of the follicles at the time of treatment and correlates with reduction in endometrial folding as well as irregularity of follicle shape. Nevertheless, hCG treatment does not influence the size and shape of preovulatory follicles or endometrial folding immediately before ovulation. Keywords: mare, induction of ovulation, ultrasonographical examination, follicle growth, follicle shape, ovulation Journal: Veterinární medicína Pages: 36-41 Volume: 57 Issue: 1 Year: 2012 DOI: 10.17221/4970-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/4970-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201201-0002.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:1:id:4970-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: P. Scheer Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Workplace-Name: International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic Author-Name: V. Sverakova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: J. Doubek Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: K. Janeckova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: I. Uhrikova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic Author-Name: P. Svoboda Author-Workplace-Name: Trauma Hospital, Brno, Czech Republic Title: Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats Abstract: This paper describes the partial results of an echocardiographic study in sixty outbreed Wistar rats. Animals of parity sex ratio were chosen for the experiment. The animals were grown up during the observation period (the minimum weight was 220 g; the maximum weight was 909 g) and were then sequentially anaesthetised (2-2.5% of isoflurane, 3 l/min O2). The second, fourth and fifth examinations were performed under anaesthesia maintained by intramuscular injections with diazepam (2 mg/kg), xylazine (5 mg/kg) and ketamine (35 mg/kg). Transthoracal examination was done using the SonoSite Titan echo system (SonoSite Ltd.) with a microconvex transducer C11 (8-5 MHz). M-mode (according to the leading-edge method of American Society of Echocardiography) echocardiography data were acquired at the papillary muscle: systolic and diastolic interventricular septum (IVSs, d) and left vetricular posterior wall (LVPWs, d) thickness, systolic and diastolic left ventricular dimension (LVDs, d), aorta (Ao) and left atrium (LA) dimensions. According to standard formulas, the following parameters were obtained: ejection fraction (EF), cardiac output (CO), stroke volume (SV), left ventricle end systolic volume (LVESV), left ventricle end diastolic volume (LVEDV), interventricular septum fractional thickening (IVSFT), left ventricular dimension fraction shortening (LVDFS), and left ventricle posterior wall fraction thickening (LVPWFS). In our study we performed 300 examinations both in male and female Wistar rats of various body weights and calculated regression equations to predict expected normal echocardiographic parameters for rats with arbitrary weights. The rats were examined by an echo scan. The first and third examinations were performed during mono-anaesthesia induced by inhalation of isoflurane. Correlations, with one exception (LVDs), were very close, which means that the results of the calculations based on regression equations are very reliable. Keywords: rat, echocardiography, M-mode, Wistar, isoflurane, diazepam, xylazine, ketamine Journal: Veterinární medicína Pages: 42-52 Volume: 57 Issue: 1 Year: 2012 DOI: 10.17221/4971-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/4971-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201201-0003.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:1:id:4971-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M.R. Farahpour Author-Workplace-Name: Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran Author-Name: M. Habibi Author-Workplace-Name: Member of Young Researchers Club, Faculty of Veterinary Medicine, Islamic Azad University, Urmia Branch, Urmia, Iran Title: Evaluation of the wound healing activity of an ethanolic extract of Ceylon cinnamon in mice Abstract: The present study was conducted to verify the effect of Ceylon cinnamon on experimentally induced excision wounds in rats. Thirty-two rats were divided into four groups of eight rats each. Group A received a placebo containing 1.5% of cinnamon and Group B a placebo containing 3%. Group C, as the control group, didn't receive any treatment and finally Group D received a blank placebo as the reference standard group. Wound healing was monitored on Days 3, 6, 9 and 14 and histological evaluation was carried out on the samples. The results show that cinnamon extract served to accelerate the wound healing process and specifically increased epithelialization in treatment groups compared to the other groups. Thus, this study demonstrates that Ceylon cinnamon may be effective in stimulating the enclosure of wounds. Keywords: wound healing, Ceylon cinnamon, ethanolic extract, mice Journal: Veterinární medicína Pages: 53-57 Volume: 57 Issue: 1 Year: 2012 DOI: 10.17221/4972-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/4972-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201201-0004.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:1:id:4972-VETMED