Template-Type: ReDIF-Article 1.0 Author-Name: L. Pavlata Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: L. Misurova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: A. Pechova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: T. Husakova Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: R. Dvorak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Direct and indirect assessment of selenium status in sheep - a comparison Abstract: The aim of this study was to determine the relationship between selenium concentration and activity of glutathione peroxidase (GSH-Px) in the whole blood of sheep and reference ranges for the activity of GSH-Px for evaluation of the selenium status of sheep in the Czech Republic. Selenium concentration and activity of glutathione peroxidase were determined in whole blood samples collected from 92 sheep in six herds. The GSH-Px activity in the samples was measured using the photometric method. Selenium in the sample was measured using the hydride technique atomic absorption spectrometry. Data on both parameters were processed using correlation and regression analysis in order to obtain reference values of GSH-Px for the indirect evaluation of the selenium status of sheep. The two variables showed a close and significant correlation (r = 0.95; P < 0.01). The regression line, defined by the equation y = 7.5857x - 121.87 (linear) or y = -0.0167x2 + 11.993x - 355.57 (polynomial), allowed us to determine the GSH-Px activity of 637, resp. 677 μkat/l as equivalent to selenium concentration in whole blood of sheep 100 μg/l. Mean selenium concentration and mean GSH-Px activity found in whole blood samples were 123.42 ± 57.84 μg/l and 814.34 ± 463.64 μkat/l, respectively. In this study, a close dependence of GSH-Px activity on the selenium concentration in the blood of sheep was found. Activity values of GSH-Px were determined for use in the diagnosis of sufficient selenium status in sheep in the Czech Republic (GSH-Px activity greater than 600 μkat/l of whole blood). Keywords: glutathione peroxidase, whole blood, trace elements, diagnostics, ruminants, white muscle disease Journal: Veterinární medicína Pages: 219-223 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5951-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5951-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0001.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5951-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: M. Adamska Author-Workplace-Name: Department of Genetics, University of Szczecin, Poland Author-Name: A. Leonska-Duniec Author-Workplace-Name: Department of Genetics, University of Szczecin, Poland Author-Name: M. Sawczuk Author-Workplace-Name: Department of Genetics, University of Szczecin, Poland Author-Name: A. Maciejewska Author-Workplace-Name: Department of Genetics, University of Szczecin, Poland Author-Name: B. Skotarczak Author-Workplace-Name: Department of Genetics, University of Szczecin, Poland Title: Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR Abstract: Cryptosporidium parvum is a common intestinal protozoan parasite infecting humans and a wide range of animals, whose diagnostics present considerable difficulties. These arise from the exceptionally robust nature of the oocyst's walls, which necessitates more stringent treatments for disruption and recovery of DNA for analysis using molecular methods. In the case of water, which is the major source of Cryptosporidium oocysts, investigations concern the detection of the presence of the oocysts. Their concentration in water is very low, and moreover, many substances that may have significance as inhibitors of DNA amplification, are present in environmental water and stool. We have carried out trials in order to assess the effectiveness of recovery of C. parvum oocysts, from spiked environmental and distilled water samples, filtrated and concentrated with the use of special laboratory equipment. Inactivation of inhibitors was carried out with use of bovine serum albumin (BSA) in PCR mixes at ten different concentrations. DNA extraction was carried out from stool samples spiked with C. parvum oocysts, concentrated using two methods, and unconcentrated. Nested PCR and a TaqMan nested real time PCR assay, targeting the 18S rRNA gene, was used to detect C. parvum DNA in spiked water and additionally in spiked stool samples. The obtained results showed that losses of C. parvum oocysts occur during the filtration and concentration of spiked water samples. The addition of small amounts of BSA (5-20 ng/µl) to PCR and TaqMan PCR mixes increases the sensitivity of both methods, but a high concentration of BSA (100 ng/µl and above) has an inhibiting effect on the polymerase reaction. The extraction of DNA from C. parvum oocysts from spiked stool samples preceded by concentration with PBS, ether and Percoll resulted in a higher copy number of the 18S rRNA gene. Keywords: Cryptosporidium parvum oocysts, spiked water and stool, water filtration, stool concentration, nested PCR, TaqMan nested real time PCR, bovine serum albumin Journal: Veterinární medicína Pages: 224-232 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5952-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5952-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0002.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5952-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: H. Wang Author-Workplace-Name: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Author-Workplace-Name: Kunming Biomed International and Yunnan Key Laboratory of Primate Biomedical Research, Kunming, China Author-Name: Y.Y. Niu Author-Workplace-Name: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Author-Workplace-Name: Kunming Biomed International and Yunnan Key Laboratory of Primate Biomedical Research, Kunming, China Author-Name: W. Si Author-Workplace-Name: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Author-Workplace-Name: Kunming Biomed International and Yunnan Key Laboratory of Primate Biomedical Research, Kunming, China Author-Name: Y.J. Li Author-Workplace-Name: Kunming Biomed International and Yunnan Key Laboratory of Primate Biomedical Research, Kunming, China Author-Name: Y. Yan Author-Workplace-Name: Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China Author-Workplace-Name: Kunming Biomed International and Yunnan Key Laboratory of Primate Biomedical Research, Kunming, China Title: Reference data of clinical chemistry, haematology and blood coagulation parameters in juvenile cynomolgus monkeys (Macaca fascicularis) Abstract: Juvenile cynomolgus monkeys are valuable models for studying human diseases. Reference data of clinical chemistry, haematology and blood coagulation parameters of juvenile cynomolgus monkeys are very important for clinical diagnosis and conducting research. In this study, 72 blood samples (obtained from 35 males and 37 females) and 20 blood samples (obtained from 10 males and 10 females) were used to determine normal data of clinical serum chemistry, haematological profiles and normal blood coagulation parameters in juvenile cynomolgus monkeys. Seventeen markers of clinical serum chemistry, twenty-nine markers of haematology and two parameters of blood coagulation were analysed. These data may provide valuable information for veterinarians and investigators using juvenile cynomolgus monkeys in research on disease treatment and in experimental studies. Keywords: serum chemical parameters, complete blood count, prothrombin time, activated partial thromboplastin time, juvenile Macaca fascicularis Journal: Veterinární medicína Pages: 233-238 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5953-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5953-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0003.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5953-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: S. Erdogan Author-Workplace-Name: Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey Title: The branching of the aortic arch in the Eurasian bittern (Botaurus stellaris, Linnaeus 1758) Abstract: This study was aimed at determining the vascular architecture of the aortic arch in the Eurasian bittern. For this purpose, the heart arteries of two bitterns were evaluated. The latex injection method was used to observe the branching of the aortic arch. Two brachiocephalic trunks were arising separately from the aortic arch and these arteries were giving to the common carotid and subclavian arteries. One of the thin branches arising from the subclavian artery was the sternoclavicular artery, which was in turn dividing into a sternal and a clavicular artery supplying the thoracic inlet and pectoral muscles. After the branching of the sternoclavicular artery, the axillary arteries were originating from the subclavian arteries. The thickest branch of the subclavian artery was the thoracic artery, which was dividing into internal and external thoracic arteries. Moreover, the brachiocephalic trunks were giving rise to the common carotid arteries and these were running cranially, giving rise to tracheosyringeal branches supplying the trachea and syrinx, thyroid artery, esophageal branches, artery of the comes nervi vagi and vertebral trunk. It is hoped that this study will enhance morphological data on exotic birds since the reports on species-specific vascular morphology in wild birds are insufficient and lacking in detail. Keywords: aortic arch, arterial branching, bittern Journal: Veterinární medicína Pages: 239-244 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5954-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5954-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0004.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5954-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: D. Vrskova Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Author-Name: H. Modra Author-Workplace-Name: University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic Title: Evaluation of the developmental toxicity of 2-phenoxyethanol and clove oil anaesthetics using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX) Abstract: The developmental toxicity of two anaesthetics, 2-phenoxyethanol and clove oil, used in aquaculture was evaluated using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX) and the results were compared to outcomes in fish. Xenopus laevis embryos were exposed to 50, 100, 300, 500, 700 and 1000 mg/l of 2-phenoxyethanol or 1, 5, 10, 20, 30 and 40 mg/l of clove oil. Values of 96 h LC50, 96 h EC50 (malformation) and teratogenic index (ratio of 96 h LC50 and 96 h EC50) were determined and the types and severities of the induced malformations and minimal concentration inhibiting the growth of embryos were estimated. Teratogenic index values for 2-phenoxy-ethanol and clove oil were estimated at 1.69 and 0.61 respectively. The most frequently observed malformations produced by 2-phenoxyethanol were axial flexure and oedema and for clove oil, axial flexure, gut malformation, microphthalmia and oedema. 2-phenoxyethanol was found to induce growth inhibition of frog embryos at concentrations above 300 mg/l and clove oil at concentrations above 20 mg/l. In summary, both 2-phenoxyethanol and clove oil affected the growth of Xenopus embryos, while only 2-phenoxyethanol represented a teratogenic risk. Keywords: eugenol, amphibian, anaesthesia, teratogenic index, malformation, African clawed frog Journal: Veterinární medicína Pages: 245-250 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5955-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5955-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0005.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5955-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I. Otrocka-Domagala Author-Workplace-Name: Veterinary Medicine Faculty, Warmia and Mazury University in Olsztyn, Poland Author-Name: Z. Procajlo Author-Workplace-Name: Veterinary Medicine Faculty, Warmia and Mazury University in Olsztyn, Poland Author-Name: K. Pazdzior Author-Workplace-Name: Veterinary Medicine Faculty, Warmia and Mazury University in Olsztyn, Poland Author-Name: M. Gesek Author-Workplace-Name: Veterinary Medicine Faculty, Warmia and Mazury University in Olsztyn, Poland Author-Name: T. Rotkiewicz Author-Workplace-Name: Veterinary Medicine Faculty, Warmia and Mazury University in Olsztyn, Poland Author-Name: W. Szweda Author-Workplace-Name: Veterinary Medicine Faculty, Warmia and Mazury University in Olsztyn, Poland Title: Immunohistochemical profile of multicentric cutaneous epitheliotropic T-cell lymphoma with generalised lymphadenopathy in a Holstein-Friesian cow: a case report Abstract: Cutaneous epitheliotropic T-cell lymphoma with progression to superficial and internal lymph nodes and the spleen was diagnosed in a two-year-old Holstein-Friesian cow. The skin lesions included multiple hypotrichous to alopecic nodules, often with ulceration, which first appeared three months after calving and progressed quickly to cover the entire body. The cow was euthanized one month later. The ELISA test excluded bovine leukaemia virus infection. Histologically in the epidermis, the formation of Pautrier's microabscesses and in the dermis, dense neoplastic infiltration with mild folliculotropism was observed. Similar neoplastic cells were present in the histological sections of lymph nodes and spleen. Immunohistochemical analysis was performed using CD3, CD79αcy, HLA-DR, WC1-N3 and Ki67 antibodies. Immunophenotyping results (CD79αcy-, CD3+, WC1-N3-) confirmed the αβ T-cell origin of neoplastic cells. The mean Ki67 index among neoplastic cells was 15.3%. On the basis of the immunohistochemical and histopathological results, the first case in Poland of cutaneous epitheliotropic T-cell lymphoma in a cow was confirmed. Additionally, MHC class II expression on approximately 10.4% of lymphoma cells was associated with a poor clinical prognosis. However, the up-regulation of MHC class II expression on accompanying cells suggested tumour immune surveillance, an antigen-specific immune response or immunosuppression. However, further investigations are needed to confirm these suspicions. Keywords: cattle, cutaneous epitheliotropic lymphoma, T-cell lymphoma, immunohistochemistry Journal: Veterinární medicína Pages: 251-257 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5956-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5956-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0006.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5956-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: I.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: C.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: K.C. Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: N.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: M.S. Kim Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Author-Name: M.R. Alam Author-Workplace-Name: Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh Author-Name: H.B. Lee Author-Workplace-Name: College of Veterinary Medicine, Chonbuk National University, Jeonju, Republic of Korea Title: Treatment of an infected total knee replacement with two-stage arthrodesis in a dog: a case report Abstract: A three-year-old intact female Great Pyrenees underwent total knee replacement for the treatment of osteoarthritis. The dog contracted septic arthritis 10 weeks following the procedure. Treatments including implant subtraction, debridement of tissue, and insertion of bone cement with antibiotics into the joint space were completed in the joint cavity. A temporary external skeletal fixator was used to stabilise the joint for four weeks. A second attempt at total knee replacement failed due to severe muscle contracture and bone loss. Therefore, it was decided to perform arthrodesis as a salvage procedure. At the 17 month follow-up examination, the patient was able to use the limb while standing and at all gait speeds. Based on these findings, two-stage arthrodesis can be a viable salvage procedure for infected total knee replacements. Keywords: total knee replacement, arthrodesis, infection, dog Journal: Veterinární medicína Pages: 258-262 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5957-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5957-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0007.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5957-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: A. Blutke Author-Workplace-Name: Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universitaet München, Munich, Germany Author-Name: J. Knebel Author-Workplace-Name: Clinic of Small Animal Surgery and Reproduction at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universitaet München, Munich, Germany Author-Name: A. Brühschwein Author-Workplace-Name: Clinic of Small Animal Surgery and Reproduction at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universitaet München, Munich, Germany Author-Name: W. Breuer Author-Workplace-Name: Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Oberschleißheim, Germany Author-Name: W. Hermanns Author-Workplace-Name: Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-Universitaet München, Munich, Germany Title: Hemangiopericytoma in a cat: a case report Abstract: In dogs and men, hemangiopericytoma is a well-recognised neoplasm, thought to originate from vascular pericytes. In cats, however, hemangiopericytoma is an extremely rare finding. The present report describes the pathological features of a 7-cm-diameter, dense, white, multilobulated tumour-like growth on the thigh of a ten-year-old Persian cat. Histologically, the mass consisted of polygonal neoplastic cells, concentrically arranged around thin-walled endothelium-lined blood vessels occasionally forming typical staghorn-configurations. In reticulin-stained sections, a dense meshwork of argyrophilic collagen fibres was evident, surrounding the central vessels and separating individual tumour cells. Within the tumour tissue, there were areas of extensive necrosis and degeneration. In the periphery, the tumour displayed a moderate infiltrative growth into the adjacent musculature. Immunohistochemical analysis revealed strong diffuse cytoplasmatic immunoreactivity for vimentin, a focal immunoreactivity for S-100, and a weak interstitial staining for laminin, whereas neoplastic cells stained negative for cytokeratin, desmin, actin, calponin, von Willebrand factor, glial fibrillary acidic protein, neuron specific enolase, CD79a, MAC387, lysozyme and MHCII. Ultrastructurally, the neoplastic cells contained few intracytoplasmatic filaments, and were surrounded by interlacing bundles of intercellular long-spacing collagen fibres. Occasionally, desmosome-like intermediate junctions were observed between neighbouring tumour cells. On the basis of histomorphology, ultrastructure and immunohistochemical reactivity, the neoplasm was diagnosed as a hemangiopericytoma, representing the second reported case of this rare tumour entity in a cat. Keywords: electron microscopy, immunohistochemistry, pericyte, perivascular, tumor Journal: Veterinární medicína Pages: 263-269 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5958-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5958-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0008.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5958-VETMED Template-Type: ReDIF-Article 1.0 Author-Name: Z. Adamiak Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: A. Pomianowski Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: I. Otrocka-Domagala Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Author-Name: M. Jaskolska Author-Workplace-Name: Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland Title: Magnetic resonance imaging of a sacral bone with telangiectatic osteosarcoma in a dog: a case report Abstract: A canine patient with osteosarcoma of the sacrum was subjected to low-field magnetic resonance imaging with the following sequences: FSE, SE, 3D HYCE, X BONE, Fast STIR, GE STIR and Gradient Echo. Due to the size and location of the tumour, surgery was not performed, and the patient was euthanized. A histopathological analysis of the tumour revealed telangiectatic osteosarcoma. Keywords: MRI, osteosarcoma, dog, spine Journal: Veterinární medicína Pages: 270-273 Volume: 57 Issue: 5 Year: 2012 DOI: 10.17221/5959-VETMED File-URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5959-VETMED.html File-Format: text/html X-File-Ref: http://agriculturejournals.cz/RePEc/caa/references/vet-201205-0009.txt Handle: RePEc:caa:jnlvet:v:57:y:2012:i:5:id:5959-VETMED